Combined testing for herpes simplex virus and Mycobacterium tuberculosis DNA in cerebrospinal fluid of patients with aseptic meningitis in Burkina Faso, West Africa.


BACKGROUND:Little is known about the involvement of herpes simplex virus (HSV) or Mycobacterium tuberculosis (MTB) as potentially curable causes of central nervous system (CNS) infections in sub-Saharan Africa. OBJECTIVE:In this study, we developed a PCR assay dedicated to simultaneous testing of HSV1/HSV2 and MTB in Burkina Faso, a country where HSV is neglected as a cause of CNS infection and where TB prevalence is high. METHODS:A consensus HSV1/HSV2 set of primers and probe were designed and combined to primers and probe targeting the IS6110 repetitive insertion sequence of MTB. Analytical performances of the assay were evaluated on reference materials. Cerebrospinal fluid (CSF) collected from subjects with aseptic meningitis was tested for HSV1/HSV2 and MTB DNA. RESULTS:The UL29 gene was chosen as a highly conserved region targeted by the HSV1/HSV2 nucleic acid test. The lower limits of detection were estimated to be 2.45 copies/µL for HSV1, 1.72 copies/µL for HSV2, and 2.54 IS6110 copies per µL for MTB. The PCR was used in 202 CSF collected from subjects suspected of aseptic meningitis. Five samples (2.46%) tested positive, including two children positive for HSV1 (0.99%) and three adults tested positive for MTB (1.47%). CONCLUSION:Using an in-house real-time PCR assay, we showed that both HSV and MTB are etiologic pathogens contributing to aseptic meningitis in Burkina Faso. This molecular test may have clinical utility for early diagnosis for those treatable CNS infections.


J Clin Lab Anal


Zida S,Kolia-Diafouka P,Kania D,Sotto A,Foulongne V,Bolloré K,Ouangraoua S,Méda N,Carrère-Kremer S,Van de Perre P,Tuaillon E




Has Abstract


2019-03-01 00:00:00












  • New method for calcium on the ADVIA analyzer is free from interference of gadolinium-type contrast agents.

    abstract::Recently, Siemens Diagnostics released a new calcium assay (CA_2) based on complex formation of calcium with Arsenazo III dye for use on the three automated, random access ADVIA Chemistry analyzers (1650, 2400, and 1200). We evaluated this method for analytical performance as well as potential interference from gadoli...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Datta P,Dasgupta A

    更新日期:2009-01-01 00:00:00

  • Effect factors related to a high probability of hemodialysis independence in newly diagnosed multiple myeloma patients requiring hemodialysis.

    abstract:BACKGROUND:Renal failure is a severe complication of symptomatic myeloma, related to higher mortality. Recovery from dialysis dependence can lead to enormous survival benefits. We investigated the effect factors for probability of dialysis independence. METHODS:Retrospective data on 45 newly diagnosed MM (NDMM) patien...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Song J,Jiang F,Liu H,Ding K,Ren Y,Li L,Wang G,Shao Z,Fu R

    更新日期:2020-02-01 00:00:00

  • High frequency of variant RHD genotypes among donors and patients of mixed origin with serologic weak-D phenotype.

    abstract:BACKGROUND:The current transfusion policy recommended for individuals with serologic weak-D phenotype is based on data derived from European-descent populations. Data referring to the distribution of RH alleles underlying weak-D phenotype among people of mixed origin are yet incomplete, and the applicability of Europea...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Dezan MR,Oliveira VB,Gomes ÇN,Luz F,Gallucci AJ,Bonifácio SL,Alencar CS,Sabino EC,Pereira AC,Krieger JE,Rocha V,Mendrone-Junior A,Dinardo CL

    更新日期:2018-11-01 00:00:00

  • Differential distribution of soluble and complexed forms of prostate-specific antigen in cyst fluids of women with gross cystic breast disease.

    abstract::Gross cystic breast disease (GCBD) is the most common benign disease of the human female breast, and patients with GCBD have an increased risk of breast cancer. The aim of this study was to evaluate the distribution inside apocrine cells and in breast cyst fluids aspirated from gross cysts of prostate-specific antigen...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Malatesta M,Mannello F,Sebastiani M,Gazzanelli G

    更新日期:2001-01-01 00:00:00

  • Simple strategy for sensitive detection of dopamine using CdTe QDs modified glassy carbon electrode.

    abstract:BACKGROUND:Cellular and brain metabolism of dopamine can be correlated with a number of neurodegenerative disorders, our study was to explore a simple and efficient method to detect dopamine in real samples. METHODS:A new quantum dots (CdTe QDs) could be prepared using the hydrothermal method, the electrochemical bios...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Yu HW,Zhang Z,Jiang JH,Pan HZ,Chang D

    更新日期:2018-03-01 00:00:00

  • Quantitative automated latex nephelometric immunoassay for determination of myoglobin in human serum.

    abstract::We have evaluated a new latex nephelometric test for the quantitation of myoglobin in human serum. The assay consists of incubating the diluted serum sample (20-fold) for 12 min at room temperature with latex particles covalently coated with anti-myoglobin antibodies and then quantifying the change of light-scatter pr...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Borque L,Rus A,Ruiz R

    更新日期:1991-01-01 00:00:00

  • Hepatitis E antibody profiles in serum and urine.

    abstract::The aim of this study was to evaluate anti-HEV antibody profiles in urine specimens in comparison to corresponding serum samples to assess the utility of urine as a clinical specimen. Paired serum and urine specimens from 71 hepatitis E patients, 33 non-E hepatitis patients, 63 patients with nonhepatic diseases, and 2...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Joshi MS,Walimbe AM,Arankalle VA,Chadha MS,Chitambar SD

    更新日期:2002-01-01 00:00:00

  • Evaluation of Toxocara cati Excretory-Secretory Larval Antigens in Serodiagnosis of Human Toxocariasis.

    abstract:BACKGROUND:Toxocariasis is the clinical term that is applied to infection in the human host with Toxocara species larvae. Serological tests are important tools for the diagnosis of toxocariasis. The aim of this study was to evaluate the excretory-secretory (ES) antigens of T. cati larvae using enzyme-linked immunosorbe...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Zibaei M,Sadjjadi SM,Sarkari B,Uga S

    更新日期:2016-05-01 00:00:00

  • AKIP1 expression in tumor tissue as a new biomarker for disease monitoring and prognosis in non-small cell lung cancer: Results of a retrospective study.

    abstract:BACKGROUND:A-kinase-interacting protein 1 (AKIP1) has been reported as an oncogenetic factor in multiple cancers; however, no study has reported its role in non-small cell lung cancer (NSCLC) yet. This study aimed to evaluate the expression of AKIP1, and its correlation with tumor characteristics as well as prognosis i...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Liu Y,Tian J,Qin D,Liu J,Xie Y

    更新日期:2020-04-01 00:00:00

  • Decreased serum exosomal miR-29a expression and its clinical significance in papillary thyroid carcinoma.

    abstract:BACKGROUND:Aberrant levels of circulating microRNAs (miRNAs) are potential biomarkers in papillary thyroid carcinoma (PTC) diagnosis and therapy. The aim of this study was to evaluate serum exosomal miR-29a expression as a non-invasive biomarker for PTC diagnosis and prognosis. METHODS:Quantitative reverse transcripti...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Wen Q,Wang Y,Li X,Jin X,Wang G

    更新日期:2020-12-23 00:00:00

  • Histocompatibility screening by molecular techniques: use of polymerase chain reaction products and heteroduplex formation.

    abstract::Novel molecular approaches have recently become available allowing improved major histocompatibility complex (MHC) matching of potential allogeneic bone marrow donors and recipients. Current cellular and serological assays are hindered by aberrant cell populations and limited reagents which only detect an individuals'...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Rubocki RJ,Wisecarver JL,Hook DD,Cox SM,Beisel KW

    更新日期:1992-01-01 00:00:00

  • New molecular diagnostic tests for two congenital forms of anemia.

    abstract::Congenital hypoplastic anemias are a rare and heterogeneous group of disorders. This paper reviews new molecular diagnostic tests in two distinct forms of congenital anemias, anemia due to transplacental infection with B19 parvovirus and Fanconi anemia. In both instances, molecular assays making use of amplification o...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章,评审


    authors: Auerbach AD,Verlander PC,Brown KE,Liu JM

    更新日期:1997-01-01 00:00:00

  • Metabolic syndrome in rheumatoid arthritis patients: Relationship among its clinical components.

    abstract:BACKGROUND:Metabolic syndrome (MetS) prevalence in rheumatoid arthritis (RA) patients is known to vary considerably across the world. This study aimed to determine the prevalence of MetS in RA patients from western Mexico and to analyze the interrelation of the MetS components with the clinical variables of RA. METHOD...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: García-Chagollán M,Hernández-Martínez SE,Rojas-Romero AE,Muñoz-Valle JF,Sigala-Arellano R,Cerpa-Cruz S,Morales-Núñez JJ,Lomelí-Nieto JA,Macedo Ojeda G,Hernández-Bello J

    更新日期:2020-11-24 00:00:00

  • Multiple cutaneous infections caused by Prototheca wickerhamii.

    abstract:BACKGROUND:Prototheca species are a group of organisms ubiquitously existing in nature but have become a pathogenic threat to public health, which has aroused wide attention. Species identification and antifungal susceptibility have essential and valuable meanings to clinical diagnosis and treatment. METHODS:A case of...

    journal_title:Journal of clinical laboratory analysis



    authors: Zhao F,Chen M,Fu Y

    更新日期:2020-11-01 00:00:00

  • Problems in determining levels of free digoxin in patients treated with digoxin immune FAb.

    abstract::Determination of free digoxin levels in patients treated with digoxin immune FAb has long been a problematic area in clinical laboratory testing. The older radioimmunoassays resulted in inaccurate and variable results due to the competition of the administered drug with the radioactively labelled forms. The 1995 Physi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Honda SA,Rios CN,Murakami L,Morita T,Scottolini AG,Bhagavan NV

    更新日期:1995-01-01 00:00:00

  • Insulin-like growth factor-II gene polymorphism is associated with primary open angle glaucoma.

    abstract::Hypoxia and ischemia play important roles in the onset and progression of glaucoma. Insulin-like growth factors (IGF) are important neurotrophic agents that respond to hypoxia-ischemia. In this study, we enrolled 60 primary open angle glaucoma (POAG) patients and 104 healthy volunteers from the China Medical College H...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Tsai FJ,Lin HJ,Chen WC,Chen HY,Fan SS

    更新日期:2003-01-01 00:00:00

  • Separation of adult chains of abnormal haemoglobin: Identification by reversed-phase high-performance liquid chromatography.

    abstract::Rare abnormal haemoglobin (Hb) often poses a problem in the diagnosis of relative electrophoresis mobility. The rare neutral Hbs interact with the Hb S and thus can increase the severity of sickle cell anaemia. In the present study, we investigated the use of reversed-phase high-performance liquid chromatography (RP-H...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Yapo PA,Datté JY,Yapo A,Wachman H

    更新日期:2004-01-01 00:00:00

  • Long non-coding RNAE330013P06 promotes progression of breast cancer with type 2 diabetes.

    abstract:BACKGROUND:In previous research, we found diabetes rather than obesity was an independent risk factor of breast cancer. However, why diabetes could lead to increased risk of breast cancer patients remains elusive. Long non-coding RNAE330013P06 has been shown to be upregulated in diabetes, and long non-coding RNAs gener...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Chen R,Shi P,Zhang Y,Wu H,Li X,Yang W,Luo F,JinmingXue,Yao L,Yang J,Wang W,Zhang B,Li P,Miao Y,Wang Q,Tian F

    更新日期:2020-05-01 00:00:00

  • The impact of vascular endothelial growth factor +405 C/G polymorphism on long-term outcome and severity of coronary artery disease.

    abstract:BACKGROUND:The association between genetic variations of vascular endothelial growth factor (VEGF) gene and the risk for atherosclerosis has been hypothesized. We aimed to assess the relationship between rs2010963 (+405 C/G) polymorphism and presence, severity, and outcome of coronary artery disease (CAD) in an Iranian...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Kalayi Nia S,Ziaee S,Boroumand MA,Sotudeh Anvari M,Pourgholi L,Jalali A

    更新日期:2017-07-01 00:00:00

  • Combination treatment of rituximab and donor platelets infusion to reduce donor-specific anti-HLA antibodies for stem cells engraftment in haploidentical transplantation.

    abstract:BACKGROUND:Donor-specific anti-human leukocyte antigen (HLA) antibodies (DSAs) in recipients is a risk factor for donor stem cell graft failure in haploidentical hematopoietic stem cell transplantation (haplo-HSCT), and the treatment to reduce the levels of DSAs is not unanimous. This study was to analysis the role of ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Zhang R,He Y,Yang D,Jiang E,Ma Q,Pang A,Zhai W,Wei J,Feng S,Han M

    更新日期:2020-07-01 00:00:00

  • Modified salting-out method for DNA isolation from newborn cord blood nucleated cells.

    abstract::The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a consi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Noguera NI,Tallano CE,Bragós IM,Milani AC

    更新日期:2000-01-01 00:00:00

  • The clinical significance of DJ-1 and HE4 in patients with endometrial cancer.

    abstract:BACKGROUND:The non-invasive diagnostic approach for early detection of endometrial cancer (EC) remains limited. To date, human epididymis protein 4 (HE4) has been intensively studied but its diagnostic is controversial in EC. DJ-1 is an oncoprotein secreted by cancer cells, recently identified as a potential diagnostic...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Benati M,Montagnana M,Danese E,Paviati E,Giudici S,Ruzzenente O,Franchi M,Lippi G

    更新日期:2018-01-01 00:00:00

  • A Comparison of Mindray BC-6800, Sysmex XN-2000, and Beckman Coulter LH750 Automated Hematology Analyzers: A Pediatric Study.

    abstract:BACKGROUND:Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Ciepiela O,Kotuła I,Kierat S,Sieczkowska S,Podsiadłowska A,Jenczelewska A,Księżarczyk K,Demkow U

    更新日期:2016-11-01 00:00:00

  • Bidirectional (negative/positive) interference of oleandrin and oleander extract on a relatively new Loci digoxin assay using Vista 1500 analyzer.

    abstract:BACKGROUND:Oleander interferes with serum digoxin measurements using various immunoassays. The potential interference of oleander and its active ingredient, oleandrin, with a relatively new homogenous sequential chemiluminescent digoxin assay based on luminescent oxygen channeling technology (LOCI digoxin assay, Siemen...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Dasgupta A,Welsh KJ,Hwang SA,Johnson M,Actor JK

    更新日期:2014-01-01 00:00:00

  • Clinical and laboratory evaluation of idiopathic male infertility in a secondary referral center in India.

    abstract::The genetic basis of infertility has received increasing recognition in recent years, particularly with the advent of assisted reproductive technology. It is now becoming obvious that genetic etiology for infertility is an important cause of disrupted spermatogenesis. Y-chromosome microdeletions and abnormal karyotype...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Abid S,Maitra A,Meherji P,Patel Z,Kadam S,Shah J,Shah R,Kulkarni V,Baburao V,Gokral J

    更新日期:2008-01-01 00:00:00

  • Circulating level of lipocalin 2 as a predictor of severity in patients with community-acquired pneumonia.

    abstract:BACKGROUND:The aim of this study was to investigate the differential plasma levels of lipocalin 2 (LCN2) and its complex with MMP-9 (where MMP is matrix metalloproteinase) before and after antibiotic treatment in hospitalized adult patients with community-acquired pneumonia (CAP). METHOD:Plasma LCN2 and LCN2/MMP-9 com...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Yeh YH,Chang JL,Hsiao PC,Tsao SM,Lin CH,Kao SJ,Chou MC,Yang SF,Chien MH

    更新日期:2013-07-01 00:00:00

  • Differentiation of Candida dubliniensis from Candida albicans on rosemary extract agar and oregano extract agar.

    abstract::Candida dubliniensis is a recently described pathogenic species which shares many phenotypic features with Candida albicans and therefore, may be misidentified in microbiological laboratories. Because molecular methods can be onerous and unfeasible in routine mycological laboratories with restricted budgets such as th...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: de Loreto ES,Pozzatti P,Alves Scheid L,Santurio D,Morais Santurio J,Alves SH

    更新日期:2008-01-01 00:00:00

  • Study of serum argininosuccinate lyase determination for diagnosis of liver diseases.

    abstract::The objectives of this research were to establish an automatic analysis method for the determination of serum argininosuccinate lyase (ASL) and to investigate the value of serum ASL test in the diagnosis of various liver disorders. According to the chemical reaction catalyzed by ASL, an enzyme-coupled reaction system ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Feng JF,Chen TM,Wen YA,Wang J,Tu ZG

    更新日期:2008-01-01 00:00:00

  • Reliability of four methods for the diagnosis of acute infection by Epstein-Barr virus.

    abstract::We studied the reliability of new indirect tests in the diagnosis of acute infection by Epstein-Barr virus (EBV). Studied for all samples were method 1, the heterophil antibodies (Abs) (Monolatex, Biokit, Germany); method 2, the IgM Abs to EBV with ELISA tests (antigen pools, Enzygnost, Behring-werke, Germany); method...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Gutiérrez J,Rodríguez M,Maroto C,Piédrola G

    更新日期:1997-01-01 00:00:00

  • Evaluation of direct agglutination test (DAT) and ELISA for serodiagnosis of visceral leishmaniasis in India.

    abstract::The direct agglutination test (DAT) and the enzyme-linked immunosorbent assay (ELISA) were used for serodiagnosis of parasitologically confirmed Indian visceral leishmaniasis (VL) cases. All the sera of VL cases were positive by both the methods. DAT titres of VL cases were greater than or equal to 1:3,200, and ELISA ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Pal A,Mukerji K,Basu D,Naskar K,Mallick KK,Ghosh DK

    更新日期:1991-01-01 00:00:00