Abstract:
:We studied a new proteinase K assay method for human serum mitochondrial aspartate aminotransferase. We found that proteinase K showed no inactivation of human mitochondrial aspartate aminotransferase isoenzyme and complete inactivation of cytosolic aspartate aminotransferase. Previous studies have shown that selective proteolytic measurement for mitochondrial aspartate aminotransferase in serum using the protease 401 cleaved peptide bond at Leu 20 from the amino-terminal bond shows complete inactivation of cytosolic aspartate aminotransferase and slight inactivation of mitochondrial aspartate aminotransferase isoenzyme, depending on protease concentration. In this investigation, we found that the proteinase K method does not depend on protease concentration. The proteinase K enzyme inactivation of cytosolic aspartate aminotransferase is caused by the cleavage of the peptide bond at Ileu 21 from the aminoterminal bond. In studies with various animal cytosolic aspartate aminotransferase isoenzymes, proteinase K almost completely inactivated cytosolic aspartate aminotransferase. Precision and correlation using proteinase K for measurement of serum mitochondrial aspartate aminotransferase in human showed a good coefficient of variation (within-run < 4.45%) and a coefficient of correlation of r = 0.985 (N = 125).
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Watazu Y,Uji Y,Okabe H,Shirahase Y,Kaneda N,Karmen Adoi
10.1002/jcla.1860070202subject
Has Abstractpub_date
1993-01-01 00:00:00pages
81-5issue
2eissn
0887-8013issn
1098-2825journal_volume
7pub_type
杂志文章abstract::A novel and sensitive noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for alpha-human atrial natriuretic peptide (alpha-hANP) in plasma, which uses only one monoclonal IgG for the ring structure of alpha-hANP, is described. Plasma was filtered through polysaccharide membrane to separate peptides...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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abstract::We have evaluated a new latex nephelometric test for the quantitation of myoglobin in human serum. The assay consists of incubating the diluted serum sample (20-fold) for 12 min at room temperature with latex particles covalently coated with anti-myoglobin antibodies and then quantifying the change of light-scatter pr...
journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.22136
更新日期:2017-11-01 00:00:00
abstract:BACKGROUND:Oleander interferes with serum digoxin measurements using various immunoassays. The potential interference of oleander and its active ingredient, oleandrin, with a relatively new homogenous sequential chemiluminescent digoxin assay based on luminescent oxygen channeling technology (LOCI digoxin assay, Siemen...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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更新日期:2018-07-01 00:00:00
abstract:BACKGROUND:This study was performed to investigate the epidemiology of enterovirus (EV) infections in children at a single hospital during recent 5 years. This study was preformed from December 2008 to November 2013. METHODS:We subjected 1,067 cerebrospinal fluid (CSF) specimens from 1,053 patients and 678 stool speci...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
pub_type: 评论,杂志文章,评审
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更新日期:2004-01-01 00:00:00
abstract::A sensitive time-resolved fluorimetric immune-complex-transfer immunoassay for antithyroglobulin IgG in serum is described. Antithyroglobulin IgG in test serum was reacted with dinitrophenyl europium ion-labeled thyroglobulin. The complex formed of antithyroglobulin IgG and dinitrophenyl europium ion-labeled thyroglob...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.1860040315
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n-propanol as internal standard. GC was performed in isothermal mode with a GC run-time of 5.0 min. ...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.21698
更新日期:2014-09-01 00:00:00
abstract::The human insulin domains, signal peptide, B-chain, C-peptide, and A-chain, were highly expressed in Escherichia coli as recombinant proteins N-terminally fused to glutathione-S-transferase and a histidine-hexapeptide. The recombinant proteins were purified from insoluble cell fraction by affinity chromatography using...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
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更新日期:1994-01-01 00:00:00
abstract:BACKGROUND:Metabolic syndrome (MetS) prevalence in rheumatoid arthritis (RA) patients is known to vary considerably across the world. This study aimed to determine the prevalence of MetS in RA patients from western Mexico and to analyze the interrelation of the MetS components with the clinical variables of RA. METHOD...
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abstract:BACKGROUND:Ischemic stroke is one of the most prominent and serious neurological complications of infective endocarditis (IE). Our study was designed to evaluate the predictive value of higher level of plasma D-dimer on admission for the development of ischemic stroke in patients with IE. METHODS:In this prospective s...
journal_title:Journal of clinical laboratory analysis
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journal_title:Journal of clinical laboratory analysis
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abstract:BACKGROUND:Kawasaki disease (KD) is an acute, self-limited vasculitis in infants and young children. Interleukin-10 (IL-10) is a potent cytokine that exerts pleiotropic effects on immunoregulation and inflammation. Elevated IL-10 serum levels have been reported in the KD patients. METHODS:In this study, we investigate...
journal_title:Journal of clinical laboratory analysis
pub_type: 杂志文章
doi:10.1002/jcla.21710
更新日期:2014-11-01 00:00:00
abstract:OBJECTIVE:Sex-determining region Y-box 30 (SOX30) suppresses progression of several cancers, whereas its role in breast cancer is unclear. Therefore, we aimed to determine the correlation of SOX30 with tumor characteristics and prognosis in breast cancer patients. METHODS:The tumor samples of 510 breast cancer patient...
journal_title:Journal of clinical laboratory analysis
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