Abstract:
BACKGROUNDS:Shortening of primary cilia in kidney epithelial cells is associated with kidney injury and involved with the induced level of α-tubulin in urine. Therefore, rapid detection and quantification of α-tubulin in the urine samples could be used to the preliminary diagnosis of kidney injury. METHODS:Cellulose-based nanobeads modified with α-tubulin were used for the detection probe of competitive immunochromatographic (IC) assay. The concentration of α-tubulin in the urine samples was determined by IC assay and compared with the amount determined by Western blotting analysis. RESULTS:The relationship between α-tubulin concentration and the colorimetric intensity resulted from IC assay was determined by logistic regression, and the correlation coefficient (R2 ) was 0.9948. When compared to the amount determined by Western blotting analysis, there was a linear relationship between the α-tubulin concentrations measured by the two methods and the R2 value was 0.823. CONCLUSIONS:This method is simple, rapid, and adequately sensitive to detect α-tubulin in patient urine samples, which could be used for the clinical diagnosis of kidney injury.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Choi ES,Al Faruque H,Kim JH,Cho JH,Park KM,Kim Edoi
10.1002/jcla.23015subject
Has Abstractpub_date
2020-01-01 00:00:00pages
e23015issue
1eissn
0887-8013issn
1098-2825journal_volume
34pub_type
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