Abstract:
:The present study was designed to evaluate whether the use of acid citrate dextrose (ACD) Formula A may enhance the survival of platelets during storage, thus allowing the continuance of platelet studies over the period of 2-3 hours usually recommended. For this purpose the effects of time on in vitro platelet response to several agonists have been investigated in platelet-rich plasma (PRP) obtained from blood samples anticoagulated with either Na citrate or ACD Formula A. The analysis of the data obtained in in vitro platelet aggregation studies using various parameters and at different time points demonstrated that storage of PRP obtained from citrated samples caused a marked reduction of platelet responses. This reduction was already evident after 6 hours, and a strong decrease was observed after 8 hours with all the agonists used. On the other hand, storage of ACD anticoagulated blood did not cause any significant decrease of platelet responsiveness up to 6 hours. A reduction of platelet aggregation became evident only after 8 hours, but not to the same extent as the one observed in citrated samples. Therefore, it may be concluded that the use of ACD Formula A as anticoagulant is capable of maintaining a normal platelet responsiveness up to 6-8 hours, thus permitting the investigation of platelet function for periods of time over those commonly recommended.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Pignatelli P,Pulcinelli FM,Ciatti F,Pesciotti M,Ferroni P,Gazzaniga PPdoi
10.1002/(SICI)1098-2825(1996)10:3<134::AID-JCLA4>3subject
Has Abstractpub_date
1996-01-01 00:00:00pages
134-9issue
3eissn
0887-8013issn
1098-2825pii
10.1002/(SICI)1098-2825(1996)10:3<134::AID-JCLA4>3journal_volume
10pub_type
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