Novel rapid immunochromatographic test based on an enzyme immunoassay for detecting nucleocapsid antigen in SARS-associated coronavirus.

abstract：:Congenital heart disease (CHD) is a common cardiac defect found in infants and children. Despite advances in diagnosis and treatment, our understanding of the causative mechanism and etiology of CHD is limited. To determine the genetic etiology of CHD, we selected 11 consecutive short tandem-repeat polymorphic (STRP) ...

journal_title：Journal of clinical laboratory analysis

authors： Shi YR,Hsieh KS,Wu JY,Lee CC,Tsai CH,Yu MT,Chang JS,Tsai FJ

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND:In previous research, we found diabetes rather than obesity was an independent risk factor of breast cancer. However, why diabetes could lead to increased risk of breast cancer patients remains elusive. Long non-coding RNAE330013P06 has been shown to be upregulated in diabetes, and long non-coding RNAs gener...

journal_title：Journal of clinical laboratory analysis

authors： Chen R,Shi P,Zhang Y,Wu H,Li X,Yang W,Luo F,JinmingXue,Yao L,Yang J,Wang W,Zhang B,Li P,Miao Y,Wang Q,Tian F

更新日期：2020-05-01 00:00:00

abstract：AIM:To evaluate, retrospectively, the frequency of autoantibodies of antiphospholipid syndrome (APLS) in Tunisian patients with primary biliary cirrhosis (PBC). PATIENTS AND METHODS:We analyzed 80 PBC sera and 80 sera from blood donors. ELISA was used to determine the frequency of antibodies against cardiolipin (aCL I...

journal_title：Journal of clinical laboratory analysis

authors： Mankaï A,Manoubi W,Ghozzi M,Melayah S,Sakly W,Ghedira I

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Though recent reports have indicated a higher prevalence of latent tuberculosis infection (LTBI) in laboratory personnel than in other healthcare workers, these studies included only a limited number of laboratory personnel. METHODS:We have thus focused on the laboratory personnel, who had a high level of e...

journal_title：Journal of clinical laboratory analysis

authors： Moon HW,Kim H,Hur M,Yun YM,Lee A

更新日期：2011-11-01 00:00:00

abstract：:A simple, rapid assay for the pancreatic isoenzyme of human serum amylase was developed. The assay utilized an immunoabsorbent prepared by coating latex beads with a monoclonal antibody specific for pancreatic amylase. Treatment of patient serum with immunoabsorbent removed pancreatic amylase, and measurement of resid...

journal_title：Journal of clinical laboratory analysis

authors： Landt M,Hortin GL,Smith CH,Pashos G,Vaidya HC,Rosenblum JL

更新日期：1994-01-01 00:00:00

abstract：BACKGROUND:It has been identified that incidence of infertility was about 20% among couples worldwide, about 50% caused by male elements. However, conventional semen laboratory detections could not handle clinical needs, which led to more comprehensive parameters for male fertility evaluation. We aimed to investigate t...

journal_title：Journal of clinical laboratory analysis

authors： Lu R,Chen X,Yu W,Jiang F,Zhou X,Xu Y,Wang F

更新日期：2020-09-14 00:00:00

abstract：:Elevated serum chromogranin A (CgA) levels have been detected in patients with prostate cancer who have developed resistance to hormonal therapy. We would like to reexamine these cases by using serial specimens to determine whether such elevated levels are also detectable in prostate cancer patients not undergoing hor...

journal_title：Journal of clinical laboratory analysis

authors： Wu JT,Wu TL,Chang CP,Tsao KC,Sun CF

更新日期：1999-01-01 00:00:00

abstract：BACKGROUND:Cognitive impairment, significantly reducing processing speed and executive function, is the critical consequence of cerebral small vessel disease (SVD), in which genetic variations have been studied. In this study, we explore the role of SNP43 (G/A) and SNP63 (C/T) polymorphism in the CAPN10 on cognitive im...

journal_title：Journal of clinical laboratory analysis

authors： Wu K,Cai Y

更新日期：2018-11-01 00:00:00

abstract：OBJECTIVE:This study aimed to investigate the predictive value of microRNA-146b (miR-146b) on acute respiratory distress syndrome (ARDS) risk, and the correlation of miR-146b with disease severity and 28-day mortality in sepsis patients. METHODS:A total of 104 sepsis patients and 100 healthy controls (HCs) were consec...

journal_title：Journal of clinical laboratory analysis

authors： Chen W,Liu L,Yang J,Wang Y

更新日期：2020-08-26 00:00:00

abstract：BACKGROUND:Circular RNAs (circRNAs) are emerging noncoding RNAs that are involved in many biological processes and diseases. The expression profile of circRNAs in preterm neonates with bronchopulmonary dysplasia (BPD) remains unresolved. METHODS:In BPD infants, peripheral venous blood was drawn and circRNAs were extra...

journal_title：Journal of clinical laboratory analysis

authors： Mao X,Guo Y,Qiu J,Zhao L,Xu J,Yin J,Lu K,Zhang M,Cheng R

更新日期：2020-07-01 00:00:00

abstract：:Polyfunctional hydrophilic microspheres of 125-nm diameter can be produced by copolymerization of acrylic monomers. Purified c-reactive protein (CRP) was covalently bound to these new micropheres, and the conjugate obtained was used as reagent in a microparticle-enhanced nephelometric immunoassay for human CRP. This a...

journal_title：Journal of clinical laboratory analysis

authors： Montagne P,Laroche P,Cuillière ML,Varcin P,Pau B,Duheille J

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:Normal serum alanine aminotransferase (ALT) levels differ with age, gender, and body mass index. Adjusting the upper limits of normal (ULN) for ALT needs further research in different populations. Aim of this work was to monitor the effect of successful chronic hepatitis C (CHC) treatment on the ALT levels i...

journal_title：Journal of clinical laboratory analysis

authors： El Kassas M,Alboraie M,Mostafa A,Ezzat R,El Tahan A,Afify S,Sweedy A,Kabbash I,Esmat G

更新日期：2018-03-01 00:00:00

abstract：BACKGROUND:Reference intervals are critical for interpreting test results of a clinical laboratory. The aim of this study was to establish local reference intervals of complete blood count for healthy preschoolers in China. METHODS:Three thousand eight hundred and twenty-one blood specimens from children aged 4 months...

journal_title：Journal of clinical laboratory analysis

authors： Wang GC,Li N,Niu C,Ma WB,Wang ZL,Guo H,Han LH

更新日期：2017-09-01 00:00:00

abstract：BACKGROUND:Coronary heart disease (CHD) is primarily caused by atherosclerosis of coronary arteries. It is largely an inflammatory disease of the vascular wall. The inflammation is related to DNA methylation. Angiopoietin-like protein 2 (ANGPTL2) has various functions in several chronic inflammatory diseases. Macrophag...

journal_title：Journal of clinical laboratory analysis

authors： Zhou J,Chen L,Yang X,Huang X,Wang Z,Peng P,Lian J

更新日期：2019-03-01 00:00:00

abstract：:To investigate the influence of race, cigarette smoking, and immunologic parameters on serum immunoglobulins, we analyzed serum IgG, IgA, and IgM levels in 455 healthy adults. The study population ranged in age from 20 to 69 years, including 282 whites and 173 blacks, 181 never-smokers, 93 ex-smokers, and 181 current ...

journal_title：Journal of clinical laboratory analysis

authors： Tollerud DJ,Brown LM,Blattner WA,Weiss ST,Maloney EM,Kurman CC,Nelson DL,Hoover RN

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND:The aim of this study was to explain the effects of microRNA-132 in renal cell carcinoma by regulating FOXM1 expression. METHODS:Thirty patients with renal cell carcinoma admitted to our hospital were enrolled, and their adjacent normal tissues and cancer tissues were taken. The expression of microRNA-132 w...

journal_title：Journal of clinical laboratory analysis

authors： Yu Y,Lu W,Zhou X,Huang H,Shen S,Guo L

更新日期：2020-01-01 00:00:00

abstract：BACKGROUND:Neurofilaments are the major cytoskeletal components of neurons, and cell injury leads to their release into the surrounding area. The aim of this study was to compare the cerebrospinal fluid (CSF) and serum (S) concentrations of neurofilament light chains (NFLs) and phosphorylated neurofilament heavy chains...

journal_title：Journal of clinical laboratory analysis

authors： Kušnierová P,Zeman D,Hradílek P,Čábal M,Zapletalová O

更新日期：2019-09-01 00:00:00

abstract：OBJECTIVES:In this paper, a novel, sensitive and rapid method to simultaneously determine the free and total prostate-specific antigen (fPSA and tPSA) in serum by combining a time-resolved fluoroimmunoassay (TRFIA) and immunomagnetic separation was described. METHODS:The new approach uses magnetic particles as an immo...

journal_title：Journal of clinical laboratory analysis

authors： Zhou B,Zhang J,Lv Z,Fan J,Zhang Y,Huang B

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:Miltenberger (Mi) series are the collective glycophorin hybrids in the MNS blood group system. Mi series are composed of several subtypes, for examples, GP.Mur, GP.Hop, and GP.Bun. The incompatibility of Mi series blood transfusion poses the risk of hemolysis. Due to the lack of standard antibodies for Mi se...

journal_title：Journal of clinical laboratory analysis

authors： Vongsakulyanon A,Pipatpanukul C,Kitpoka P,Kunakorn M,Srikhirin T

更新日期：2016-11-01 00:00:00

abstract：AIM:The present study aimed to investigate associations of the platelet-to-white blood cell ratio (PWR)-a novel hematological indicator of inflammatory responses-with 30-day outcomes in patients with HBV-associated decompensated cirrhosis (HBV-DeCi). METHODS:We recruited 131 patients with HBV-DeCi for this retrospecti...

journal_title：Journal of clinical laboratory analysis

authors： Zhang J,Qiu Y,He X,Mao W,Han Z

更新日期：2020-09-07 00:00:00

abstract：:It is believed that boundary compositions of matrix proteins might play a role in stone formation; however, few proteomic studies concerning matrix proteins in urinary stones have been conducted. In this study, we extracted low molecular weight proteins from calcium oxalate stones and measured their characteristic pat...

journal_title：Journal of clinical laboratory analysis

authors： Chen WC,Lai CC,Tsai Y,Lin WY,Tsai FJ

更新日期：2008-01-01 00:00:00

abstract：:It has been proposed that sample storage may have some influence on the parameters of oxidative stress status (OSS) in biological fluids. We measured four important OSS parameters in plasma of 23 healthy subjects and repeated the measurements in the same samples kept at -70 degrees C after different time intervals. Hy...

journal_title：Journal of clinical laboratory analysis

authors： Firuzi O,Mladenka P,Riccieri V,Spadaro A,Petrucci R,Marrosu G,Saso L

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Identification of dermatophytes at the species level, relying on macro- and microscopic properties of the colonies is time-consuming, questioned in many circumstances, and requires considerable expertise. In this study, we examined the potency of a new genetic marker, β-tubulin (BT2) gene, for differentiatio...

journal_title：Journal of clinical laboratory analysis

authors： Abastabar M,Mirhendi H,Rezaei-Matehkolaei A,Shidfar MR,Kordbacheh P,Makimura K

更新日期：2014-03-01 00:00:00

abstract：BACKGROUND:Low concentration C-reactive protein (CRP) has favorable prognostic significance in patients with cardiovascular risks. METHODS:We compared the wr-CRP method with the hs-CRP method both on Roche Cobas c702 analyzer for the determination of low CRP concentration (<20 mg/L) including 200 patients treated in C...

journal_title：Journal of clinical laboratory analysis

authors： Li R,Xue Y,Wang T,Gong L,Peng P,Xiong P,Dai M,Shao T,Hu Y,Ye X

更新日期：2019-10-01 00:00:00

abstract：BACKGROUND:So far, there has been no tool to estimate activity at diagnosis and predict all-cause mortality in patients with ANCA-associated vasculitis (AAV). Hence, we determined the initial predictors of them in patients with AAV. METHODS:We retrospectively reviewed the medical records of 182 patients with AAV. Seve...

journal_title：Journal of clinical laboratory analysis

authors： Kim MK,Choi H,Kim JY,Song JJ,Park YB,Lee SW

更新日期：2020-01-01 00:00:00

abstract：:Urinary proteins from six patients with esophageal cancer and two with stomach cancer were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analyses were performed on days-1 to 3, 5, 7, 10, 14, and 21 (or 22) after surgery. The protein patterns were scanned by densitometry and divided ...

journal_title：Journal of clinical laboratory analysis

authors： Yokomizo K,Iijima S,Sakai N,Kurihara Y,Hitratsuka N,Nagai K,Iwai T,Shiba K

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:Iron overload is a major complication in patients with hemoglobin H (Hb H) disease and causes damage of tissues. METHODS:We investigated 26 Hb H patients and 75 controls to evaluate their oxidative stress and antioxidant statuses. RESULTS:There were significantly increased levels of superoxide anion in leu...

journal_title：Journal of clinical laboratory analysis

authors： Chiou SS,Tsao CJ,Tsai SM,Wu YR,Liao YM,Lin PC,Tsai LY

更新日期：2014-07-01 00:00:00

abstract：BACKGROUND:Enzyme-linked immunosorbent assay (ELISA) has traditionally been used to detect myeloperoxidase (MPO) and proteinase 3 (PR3) antibodies, although it is time-consuming and physically demanding. As a novel and highly effective immunoassay, we compared chemiluminescent immunoassay (CIA) with ELISA to verify the...

journal_title：Journal of clinical laboratory analysis

authors： Hou X,Liu J,Wang T,Zhou J,Cui L

更新日期：2020-10-09 00:00:00

abstract：BACKGROUND:The pulmonary surfactant especially lipids in amniotic fluid can reflect the development stage of fetal lung maturity (FLM). However, the conventional lecithin/sphingomyelin (L/S) ratio method by thin layer chromatography (TLC) is insufficient and inconvenient for FLM prediction in clinical practice. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Cao Z,Liu J,Xie X,Zhan S,Song W,Wu S,Sun Z,Dong Y,Tang G,Liu Y,Li L,Shen M,Zhai Y,Zou J,Liu X

更新日期：2020-04-01 00:00:00

abstract：BACKGROUND:Although it is widely acknowledged that physical activity confers several health benefits, it remains uncertain whether strenuous and physically demanding exercise might determine biological effects that might turn to be ultimately unfavorable for health. Copeptin and midregion proadrenomedullin (MR-proADM) ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Schena F,Salvagno GL,Sanchis-Gomar F,Guidi GC

更新日期：2015-01-01 00:00:00