Metabolic pathways related to oxidative stress in patients with hemoglobin h disease and iron overload.


BACKGROUND:Iron overload is a major complication in patients with hemoglobin H (Hb H) disease and causes damage of tissues. METHODS:We investigated 26 Hb H patients and 75 controls to evaluate their oxidative stress and antioxidant statuses. RESULTS:There were significantly increased levels of superoxide anion in leucocytes, nitrite (NO2-), and malondialdehyde (MDA) in plasma, and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRx) and oxidized glutathione (GSSG) in erythrocytes, decreased levels of nitrate (NO3-) and vitamin C in plasma, and reduced glutathione (GSH) in erythrocytes, in addition to the abnormal iron status in the patients when compared with those in the controls. Meanwhile, levels of serum ferritin were positively correlated with serum iron, plasma MDA, and erythrocyte SOD in the patients. In addition, the activities of SOD were positively correlated with those of GPx and GRx, and the levels of GSSG and MDA, but negatively correlated with those of GSH. Furthermore, the levels of MDA were negatively correlated those of vitamin C. CONCLUSIONS:These results demonstrate the presence of oxidative stress and decreased levels of antioxidants; moreover, the related metabolic antioxidant pathway is active in Hb H patients with iron overload.


J Clin Lab Anal


Chiou SS,Tsao CJ,Tsai SM,Wu YR,Liao YM,Lin PC,Tsai LY




Has Abstract


2014-07-01 00:00:00












  • Serum lipid hydroperoxide levels and paraoxonase activity in patients with lung, breast, and colorectal cancer.

    abstract:OBJECTIVES:The objectives of this article are to investigate the serum lipid hydroperoxide (LOOH) levels and paraoxonase-1 (PON1) and arylesterase (ARE) activity in patients with lung, breast, and colorectal cancer. DESIGN AND METHODS:Serum PON1 and ARE activities and LOOH levels were measured in 110 patients with can...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Balci H,Genc H,Papila C,Can G,Papila B,Yanardag H,Uzun H

    更新日期:2012-05-01 00:00:00

  • Factors interfering with the accuracy of five blood glucose meters used in Chinese hospitals.

    abstract:BACKGROUND:The prevalence of diabetes is increasing in China. Glucose control is very important in diabetic patients. The aim of this study was to compare the accuracy of five glucose meters used in Chinese hospitals with a reference method, in the absence and presence of various factors that may interfere with the met...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Lv H,Zhang GJ,Kang XX,Yuan H,Lv YW,Wang WW,Randall R

    更新日期:2013-09-01 00:00:00

  • Does polycythemia affect interleukin-6 response pattern in early postnatal period?

    abstract:INTRODUCTION:Neonatal polycythemia may result in increased cytokine production. We aimed to investigate whether polycythemia and partial exchange transfusion (PET) affect interleukin-6 (IL-6) response pattern in early neonatal period. METHODS:Ninety-four newborns, 57 with polycythemia (Group 1) and 37 as control group...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Akdag A,Dilli D,Erdeve O,Oğuz SS,Dilmen U

    更新日期:2010-01-01 00:00:00

  • Simultaneous determination of free and total prostate-specific antigen by a magnetic particle-based time-resolved fluoroimmunoassay.

    abstract:OBJECTIVES:In this paper, a novel, sensitive and rapid method to simultaneously determine the free and total prostate-specific antigen (fPSA and tPSA) in serum by combining a time-resolved fluoroimmunoassay (TRFIA) and immunomagnetic separation was described. METHODS:The new approach uses magnetic particles as an immo...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Zhou B,Zhang J,Lv Z,Fan J,Zhang Y,Huang B

    更新日期:2017-11-01 00:00:00

  • The impact of vascular endothelial growth factor +405 C/G polymorphism on long-term outcome and severity of coronary artery disease.

    abstract:BACKGROUND:The association between genetic variations of vascular endothelial growth factor (VEGF) gene and the risk for atherosclerosis has been hypothesized. We aimed to assess the relationship between rs2010963 (+405 C/G) polymorphism and presence, severity, and outcome of coronary artery disease (CAD) in an Iranian...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Kalayi Nia S,Ziaee S,Boroumand MA,Sotudeh Anvari M,Pourgholi L,Jalali A

    更新日期:2017-07-01 00:00:00

  • A Comparison of Mindray BC-6800, Sysmex XN-2000, and Beckman Coulter LH750 Automated Hematology Analyzers: A Pediatric Study.

    abstract:BACKGROUND:Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Ciepiela O,Kotuła I,Kierat S,Sieczkowska S,Podsiadłowska A,Jenczelewska A,Księżarczyk K,Demkow U

    更新日期:2016-11-01 00:00:00

  • An improved microalbumin method (microALB_2) with extended analytical measurement range evaluated on the ADVIA chemistry systems.

    abstract::Quantitative determination of albumin (ALB) in human urine is important to assess kidney functions in a variety of diseases. Recently, Siemens released an improved Microalbumin assay (microALB_2) to measure urinary ALB on the automated, random access ADVIA 1650/1800, ADVIA 2400, and ADVIA 1200 Chemistry Systems. We ev...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Datta P,Dasgupta A

    更新日期:2009-01-01 00:00:00

  • Identical IgM antibodies recognizing a glycine-alanine epitope are induced during acute infection with Epstein-Barr virus and cytomegalovirus.

    abstract::We studied antibody production in serial serum samples from patients with acute Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infections. Sera were analyzed both by enzyme-linked immunosorbent assay (ELISA) using a synthetic peptide (P62) derived from the glycine-alanine repeating region of the Epstein-Barr nucle...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Rhodes G,Smith RS,Rubin RE,Vaughan J,Horwitz CA

    更新日期:1990-01-01 00:00:00

  • The development and organization of newborn screening programs in Turkey.

    abstract:BACKGROUND:Newborn screening tests have been designed to identify infants with severe disorders that are relatively prevalent and treatable or controllable. Comparing to other countries, the incidence of these diseases are very high in Turkey where the rate of consanguineous marriage is high. METHODS:In this article, ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Tezel B,Dilli D,Bolat H,Sahman H,Ozbaş S,Acıcan D,Ertek M,Köse MR,Dilmen U

    更新日期:2014-01-01 00:00:00

  • Molecular forms of soluble human T lymphocyte receptor for sheep erythrocytes in serum and saliva.

    abstract::Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Itano EN,Ono MA,Sumigawa M,Longo IM,Moura NC,Mendes NF

    更新日期:1991-01-01 00:00:00

  • Problems in determining levels of free digoxin in patients treated with digoxin immune FAb.

    abstract::Determination of free digoxin levels in patients treated with digoxin immune FAb has long been a problematic area in clinical laboratory testing. The older radioimmunoassays resulted in inaccurate and variable results due to the competition of the administered drug with the radioactively labelled forms. The 1995 Physi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Honda SA,Rios CN,Murakami L,Morita T,Scottolini AG,Bhagavan NV

    更新日期:1995-01-01 00:00:00

  • MicroRNA-146b correlates with decreased acute respiratory distress syndrome risk, reduced disease severity, and lower 28-day mortality in sepsis patients.

    abstract:OBJECTIVE:This study aimed to investigate the predictive value of microRNA-146b (miR-146b) on acute respiratory distress syndrome (ARDS) risk, and the correlation of miR-146b with disease severity and 28-day mortality in sepsis patients. METHODS:A total of 104 sepsis patients and 100 healthy controls (HCs) were consec...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Chen W,Liu L,Yang J,Wang Y

    更新日期:2020-08-26 00:00:00

  • Decrease of HCVRNA after three days of daily interferon treatment is predictive of the virological response at one month.

    abstract::Early monitoring of HCVRNA during interferon treatment may allow clinicians to obtain important information that could help them to adopt therapeutic decisions in individual cases. The hepatitis C virus infection is highly dynamic and a daily high dose of IFN may induce a decline of viremia of 95+/-10% of baseline val...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 临床试验,杂志文章


    authors: Magalini A,Puoti M,Putzolu V,Quiros-Roldan E,Forleo MA,Rossi S,Zaltron S,Spinetti A,Zanini B,Zonaro A,Solfrini R,Carosi G

    更新日期:2000-01-01 00:00:00

  • Analysis of serum gastrin-17 and Helicobacter pylori antibody in healthy Chinese population.

    abstract:BACKGROUND:Gastrin-17 (G-17) and Helicobacter pylori (H pylori) antibody are widely used in the screening of gastric diseases, especially in gastric cancer. In this study, we aimed to evaluate the value of G-17 and H pylori antibody in gastric disease screening. METHODS:Healthy males and females (1368 and 1212, respec...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Liu W,Sun Y,Yuan Y

    更新日期:2020-08-30 00:00:00

  • Association between blood microbiome and type 2 diabetes mellitus: A nested case-control study.

    abstract:BACKGROUND:Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce. METHODS:Fifty incident T2DM cases and 100 matched non-T2DM controls were selected fr...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Qiu J,Zhou H,Jing Y,Dong C

    更新日期:2019-05-01 00:00:00

  • Multiplex assay for genetic testing of thrombophilia: a method for routine clinical care.

    abstract::There is an increasing demand for genetic testing of patients with hypercoagulability. We have developed a multiplex-polymerase chain reaction (PCR) technique that incorporates the state-of-the art of genetic testing for thrombosis mutations (Factor V Leiden [FVL] and G20210A F2 and C46T F12). The sequences are detect...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: López M,Giraldo P,Alvarez P,Cornudella R,Pocoví M,Martínez A,Fontcuberta J,Soria JM

    更新日期:2007-01-01 00:00:00

  • Comparison of various methods for the enumeration of blood cells in urine.

    abstract::This study compared the use of conventional urine microscopy, the KOVA system, Neubauer hemacytometry, and the Yellow IRIS for the determination of white blood cell (WBC) and red blood cell (RBC) counts in urine samples. Both KOVA WBC and RBC counts correlated better with the IRIS counts than with conventional microsc...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: McGinley M,Wong LL,McBride JH,Rodgerson DO

    更新日期:1992-01-01 00:00:00

  • A proposal for standardizing urine collections for bone resorption markers measurement.

    abstract::Diurnal variations in the excretion of bone resorption markers were assessed in order to identify the type of urine collection which provides the most information on bone resorption rate and its relation to measuring bone dynamics in a postmenopausal population. Sixty women, ages 43-67 and without disease or treatment...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Zaninotto M,Bernardi D,Ujka F,Bonato P,Plebani M

    更新日期:1998-01-01 00:00:00

  • Modified salting-out method for DNA isolation from newborn cord blood nucleated cells.

    abstract::The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a consi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Noguera NI,Tallano CE,Bragós IM,Milani AC

    更新日期:2000-01-01 00:00:00

  • Up-regulation of circular RNA hsa_circ_0037909 promotes essential hypertension.

    abstract:AIMS:Essential hypertension (EH) is a high prevalence disease facing a public health challenge. People were little known about the genetics of diagnosing the cause of EH. Circular RNAs that have a continuous cycle of covalent closure, without affected by RNA exonuclease, and are more stable and hard to degrade may invo...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Bao X,He X,Zheng S,Sun J,Luo Y,Tan R,Zhao J,Zhong F,Zhang L

    更新日期:2019-05-01 00:00:00

  • Colorimetric Detection by Gold Nanoparticle DNA Probes for Miltenberger Series (GP.Mur, GP.Hop, and GP.Bun) Identification.

    abstract:BACKGROUND:Miltenberger (Mi) series are the collective glycophorin hybrids in the MNS blood group system. Mi series are composed of several subtypes, for examples, GP.Mur, GP.Hop, and GP.Bun. The incompatibility of Mi series blood transfusion poses the risk of hemolysis. Due to the lack of standard antibodies for Mi se...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Vongsakulyanon A,Pipatpanukul C,Kitpoka P,Kunakorn M,Srikhirin T

    更新日期:2016-11-01 00:00:00

  • MicroRNA-769-5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer.

    abstract:BACKGROUND:Nucleolar and spindle-associated protein 1 (NUSAP1) has been identified to be strongly implicated in the carcinogenesis of cervical carcinoma, breast cancer, and liver cancer, and shows a high expression level in bladder cancer, indicating that NUSAP1 might be a potent target for cancer treatment. Using bioi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Chen Y,Zhang W,Kadier A,Zhang H,Yao X

    更新日期:2020-05-01 00:00:00

  • Reference Intervals of and Relationships among Essential Trace Elements in Whole Blood of Children Aged 0-14 years.

    abstract:BACKGROUND:Essential trace elements are vital to human health. In this study, our aim was to establish reference intervals of and to evaluate relationships among Ca, Fe, Cu, Mg, and Zn for children. METHODS:We collected blood samples of 3210 children aged 0-14 years from Lu'an, China, and concentrations of the above e...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Zhai R,Zhang M,Liu J,Guang H,Li B,Chen D,Zhang S

    更新日期:2017-03-01 00:00:00

  • Measurement of serum IgA and C3 may predict the diagnosis of patients with IgA nephropathy prior to renal biopsy.

    abstract::The levels of serum IgA and C3 in patients with IgA nephropathy were determined using international standard serum (IFCC/CRM470) in a multicenter trial in Japan. The ratio of serum IgA to C3 (serum IgA/C3 ratio) without any information from renal biopsy was used for the diagnosis of IgA nephropathy. Three hundred and ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Tomino Y,Suzuki S,Imai H,Saito T,Kawamura T,Yorioka N,Harada T,Yasumoto Y,Kida H,Kobayashi Y,Endoh M,Sato H,Saito K

    更新日期:2000-01-01 00:00:00

  • Reliability of four methods for the diagnosis of acute infection by Epstein-Barr virus.

    abstract::We studied the reliability of new indirect tests in the diagnosis of acute infection by Epstein-Barr virus (EBV). Studied for all samples were method 1, the heterophil antibodies (Abs) (Monolatex, Biokit, Germany); method 2, the IgM Abs to EBV with ELISA tests (antigen pools, Enzygnost, Behring-werke, Germany); method...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Gutiérrez J,Rodríguez M,Maroto C,Piédrola G

    更新日期:1997-01-01 00:00:00

  • Multiple cutaneous infections caused by Prototheca wickerhamii.

    abstract:BACKGROUND:Prototheca species are a group of organisms ubiquitously existing in nature but have become a pathogenic threat to public health, which has aroused wide attention. Species identification and antifungal susceptibility have essential and valuable meanings to clinical diagnosis and treatment. METHODS:A case of...

    journal_title:Journal of clinical laboratory analysis



    authors: Zhao F,Chen M,Fu Y

    更新日期:2020-11-01 00:00:00

  • Genetic analysis of chromosome 22q11.2 markers in congenital heart disease.

    abstract::Congenital heart disease (CHD) is a common cardiac defect found in infants and children. Despite advances in diagnosis and treatment, our understanding of the causative mechanism and etiology of CHD is limited. To determine the genetic etiology of CHD, we selected 11 consecutive short tandem-repeat polymorphic (STRP) ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Shi YR,Hsieh KS,Wu JY,Lee CC,Tsai CH,Yu MT,Chang JS,Tsai FJ

    更新日期:2003-01-01 00:00:00

  • Metabolic syndrome in rheumatoid arthritis patients: Relationship among its clinical components.

    abstract:BACKGROUND:Metabolic syndrome (MetS) prevalence in rheumatoid arthritis (RA) patients is known to vary considerably across the world. This study aimed to determine the prevalence of MetS in RA patients from western Mexico and to analyze the interrelation of the MetS components with the clinical variables of RA. METHOD...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: García-Chagollán M,Hernández-Martínez SE,Rojas-Romero AE,Muñoz-Valle JF,Sigala-Arellano R,Cerpa-Cruz S,Morales-Núñez JJ,Lomelí-Nieto JA,Macedo Ojeda G,Hernández-Bello J

    更新日期:2020-11-24 00:00:00

  • Hormonal modulation of the vaginal bacterial flora in experimental polycystic ovarian disease.

    abstract::Rats exposed to constant light develop polycystic ovarian (PCO) disease with persistent estrus, representing an estrogen-dominant condition. Herein, we report that fluctuations seen in the vaginal microflora in cyclic rats were not observed in PCO rats with persistent estrus. The vaginal-cervical mucosa of PCO rats sh...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Singh KB,Mahajan DK,Tewari RP

    更新日期:1996-01-01 00:00:00

  • Microparticle-enhanced nephelometric immunoassay of human plasminogen.

    abstract::A microparticle-enhanced nephelometric immunoassay was developed for plasminogen quantitation in human plasma. It is based on the nephelometric measurement of the light scattered by microparticle clusters formed during a sandwich reaction between plasminogen, microparticle--anti-plasminogen conjugate, and the free ant...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Marchal E,Montagne P,Cuillière ML,Béné MC,Faure G

    更新日期:1996-01-01 00:00:00