Metabolic pathways related to oxidative stress in patients with hemoglobin h disease and iron overload.

abstract：BACKGROUND:Kawasaki disease (KD) is an acute, self-limited vasculitis in infants and young children. Interleukin-10 (IL-10) is a potent cytokine that exerts pleiotropic effects on immunoregulation and inflammation. Elevated IL-10 serum levels have been reported in the KD patients. METHODS:In this study, we investigate...

journal_title：Journal of clinical laboratory analysis

authors： Lin YJ,Lan YC,Lai CH,Lin TH,Huang SM,Liao CC,Lin CW,Hung CH,Tien N,Liu X,Chien WK,Chen JH,Tsai FJ

更新日期：2014-11-01 00:00:00

abstract：OBJECTIVE:A common problem in clinical laboratories is maintaining the stability of analytes during pre-analytical processes. The aim of this study was to systematically summarize the results of a set of studies about the biochemical analytes stability. METHODS:A literature search was performed on the Advanced search ...

journal_title：Journal of clinical laboratory analysis

authors： Hedayati M,Razavi SA,Boroomand S,Kheradmand Kia S

更新日期：2020-09-01 00:00:00

abstract：BACKGROUND:Early recognition and treatment for severe drug eruption are important in improving clinical outcomes. A few studies have reported laboratory parameters to evaluate the severity of drug eruptions. This study aimed to determine the association between serum ferritin and the severity of drug eruptions. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Pan Y,Tang S,Zheng S,Li S,Zhu D,Fang H,Qiao J

更新日期：2020-02-01 00:00:00

abstract：:A radioactive polymerase chain reaction (PCR) method has been developed for the relative quantification of the human alpha-2 chain of type I collagen [hu alpha-2(I)] in cells. cDNAs generated by reverse transcription from the total pool of cytoplasmic RNA serve as a template for polymerase chain reaction amplification...

journal_title：Journal of clinical laboratory analysis

authors： Yu YL,Golden JA,Migchielsen AA,Goetzl EJ,Turck CW

更新日期：1991-01-01 00:00:00

abstract：:Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), which are most abundant in the liver, are the main enzymes involved in ethanol metabolism in humans. Gender-related differences in total liver ADH and ALDH activity among different animal species have been observed in many studies. We measured total ADH an...

journal_title：Journal of clinical laboratory analysis

authors： Chrostek L,Jelski W,Szmitkowski M,Puchalski Z

更新日期：2003-01-01 00:00:00

abstract：AIM:To investigate the presence of antipituitary antibodies (APA) in the serum of patients undergoing gastroenteropathies (GEP) other than celiac disease (CD). METHODS:APA were analyzed in GEP patients (n = 103), CD patients (n = 94), idiopathic growth hormone (GH) deficiency patients (n = 21), and 98 age- and sex-mat...

journal_title：Journal of clinical laboratory analysis

authors： Aguado R,Fernández S,Estévez OA,Santamaría M,Ortega C

更新日期：2014-01-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum-absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum-absorbed te...

journal_title：Journal of clinical laboratory analysis

authors： Thiele GM,Rogers J,Collins M,Yasuda N,Smith D,McDonald TL

更新日期：1990-01-01 00:00:00

abstract：:Vitamin D plays a vital role not only in bone health but also in pathophysiology of many other body functions. In recent years, there has been significant increase in testing of 25-hydroxyvitamin D (25-OH vitamin D), a marker of vitamin D deficiency. The most commonly used methods for the measurement of 25-OH vitamin ...

journal_title：Journal of clinical laboratory analysis

authors： Garg U,Munar A,Frazee C 3rd,Scott D

更新日期：2012-09-01 00:00:00

abstract：BACKGROUND:Cellular and brain metabolism of dopamine can be correlated with a number of neurodegenerative disorders, our study was to explore a simple and efficient method to detect dopamine in real samples. METHODS:A new quantum dots (CdTe QDs) could be prepared using the hydrothermal method, the electrochemical bios...

journal_title：Journal of clinical laboratory analysis

authors： Yu HW,Zhang Z,Jiang JH,Pan HZ,Chang D

更新日期：2018-03-01 00:00:00

abstract：:Our objectives in this study were to compare results obtained by an enzyme immunoassay (EIA) for F-actin antibody (FAA) immunoglobulin G (IgG) to those determined by an indirect fluorescent antibody (IFA) assay for smooth muscle antibody (SMA) IgG, and to determine the prevalence of FAA in patient sera having serologi...

journal_title：Journal of clinical laboratory analysis

authors： Jaskowski TD,Konnick EQ,Ashwood ER,Litwin CM,Hill HR

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Cell-free DNA (cfDNA) in the plasma of patients with both malignant and benign breast lesions was analyzed to determine whether the findings may have diagnostic and prognostic implications and to analyze the association between the levels of cfDNA and prognostic parameters. METHODS:Plasma samples were obtai...

journal_title：Journal of clinical laboratory analysis

authors： Hashad D,Sorour A,Ghazal A,Talaat I

更新日期：2012-11-01 00:00:00

abstract：BACKGROUND:Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray ...

journal_title：Journal of clinical laboratory analysis

authors： Ciepiela O,Kotuła I,Kierat S,Sieczkowska S,Podsiadłowska A,Jenczelewska A,Księżarczyk K,Demkow U

更新日期：2016-11-01 00:00:00

abstract：:Elevated serum chromogranin A (CgA) levels have been detected in patients with prostate cancer who have developed resistance to hormonal therapy. We would like to reexamine these cases by using serial specimens to determine whether such elevated levels are also detectable in prostate cancer patients not undergoing hor...

journal_title：Journal of clinical laboratory analysis

authors： Wu JT,Wu TL,Chang CP,Tsao KC,Sun CF

更新日期：1999-01-01 00:00:00

abstract：BACKGROUND:Donor-specific anti-human leukocyte antigen (HLA) antibodies (DSAs) in recipients is a risk factor for donor stem cell graft failure in haploidentical hematopoietic stem cell transplantation (haplo-HSCT), and the treatment to reduce the levels of DSAs is not unanimous. This study was to analysis the role of ...

journal_title：Journal of clinical laboratory analysis

authors： Zhang R,He Y,Yang D,Jiang E,Ma Q,Pang A,Zhai W,Wei J,Feng S,Han M

更新日期：2020-07-01 00:00:00

abstract：:The experimental and clinical effectiveness of nasal salmon calcitonin (SCT) for treatment of osteoporosis in humans has been well established, but none is known yet about the pharmacokinetic property in relation to therapeutic efficacy, especially when used in a therapeutic dose range. This preclinical study was desi...

journal_title：Journal of clinical laboratory analysis

authors： Nakamuta H,Kohno T,Ichikawa M,Hoshino T,Watabe K,Koida M

更新日期：1997-01-01 00:00:00

abstract：BACKGROUND:Contradictory results have been reported previously in the analyses of cross-reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co-sensitization and the IgE cross-reactivity among them...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Zheng P,Zheng SG,Zhai Y,Zhao X,Chen Y,Cai C,Wu Z,Huang Z,Zou X,Liao C,Sun B

更新日期：2019-11-01 00:00:00

abstract：OBJECTIVE:To explore relationships between immunological status, clinical features, radiographic damage, disease activity, and functional disability in Tunisian patients with rheumatoid arthritis (RA). MATERIALS AND METHODS:The study was carried out in 112 patients with RA. Demographic characteristics, disease duratio...

journal_title：Journal of clinical laboratory analysis

authors： Hamad MB,Marzouk S,Kaddour N,Masmoudi H,Fakhfakh F,Rebai A,Bahloul Z,Maalej A

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:Nucleolar and spindle-associated protein 1 (NUSAP1) has been identified to be strongly implicated in the carcinogenesis of cervical carcinoma, breast cancer, and liver cancer, and shows a high expression level in bladder cancer, indicating that NUSAP1 might be a potent target for cancer treatment. Using bioi...

journal_title：Journal of clinical laboratory analysis

authors： Chen Y,Zhang W,Kadier A,Zhang H,Yao X

更新日期：2020-05-01 00:00:00

abstract：:The two serum proteins of the complement cascade in the highest concentrations, C3 and C4, respond to various conditions in much the same manner as do other positive acute-phase proteins. A major difference is that they are relatively sluggish in response to cytokine drive, requiring several days rather than hours to ...

journal_title：Journal of clinical laboratory analysis

authors： Ritchie RF,Palomaki GE,Neveux LM,Navolotskaia O,Ledue TB,Craig WY

更新日期：2004-01-01 00:00:00

abstract：:A microparticle-enhanced nephelometric immunoassay was developed for plasminogen quantitation in human plasma. It is based on the nephelometric measurement of the light scattered by microparticle clusters formed during a sandwich reaction between plasminogen, microparticle--anti-plasminogen conjugate, and the free ant...

journal_title：Journal of clinical laboratory analysis

authors： Marchal E,Montagne P,Cuillière ML,Béné MC,Faure G

更新日期：1996-01-01 00:00:00

abstract：OBJECTIVE:The study aimed to explore the correlations of long non-coding RNA MALAT1 (lncRNA MALAT1) and its targets microRNA (miR)-125b, miR-133, miR-146a, and miR-203 with acute exacerbation risk, inflammation, and disease severity of chronic obstructive pulmonary disease (COPD). METHODS:Plasma samples were obtained ...

journal_title：Journal of clinical laboratory analysis

authors： Liu S,Liu M,Dong L

更新日期：2020-09-01 00:00:00

abstract：BACKGROUND:Selection and verification of blood collection tubes is an important preanalytical issue in clinical laboratories. Today, gel tubes are commonly used with many advantages, although they are known to cause interference in immunoassay methods. In this study, we aimed to compare SSTs of two different suppliers ...

journal_title：Journal of clinical laboratory analysis

authors： Ercan M,Fırat Oğuz E,Akbulut ED,Yilmaz M,Turhan T

更新日期：2018-07-01 00:00:00

abstract：:The use of serum alkaline phosphatase (ALP) isoenzymes as markers of breast cancer metastases and treatment efficacy has received little attention. Twenty-six breast cancer women (56+/-13 years, all post-menopausal) were prospectively evaluated during their first and third course of chemotherapy (4-week interval). Ser...

journal_title：Journal of clinical laboratory analysis

authors： Le Bricon T,Gay-Bellile C,Cottu P,Benlakehal M,Guillon H,Houzé P

更新日期：2010-01-01 00:00:00

abstract：:Quantitation of hemoglobin alpha 2 delta 2 (HbA2) is a basic and confirmatory test in diagnosing the carrier state of beta-thalassemia. The present study was designed to investigate the effect of cigarette smoking on the diagnostic reliability of HbA2. A total of 2,867 (654 smokers and 2,213 never smokers) male subjec...

journal_title：Journal of clinical laboratory analysis

authors： Tarazi IS,Sirdah MM,El Jeadi H,Al Haddad RM

更新日期：2008-01-01 00:00:00

abstract：:Congenital hypoplastic anemias are a rare and heterogeneous group of disorders. This paper reviews new molecular diagnostic tests in two distinct forms of congenital anemias, anemia due to transplacental infection with B19 parvovirus and Fanconi anemia. In both instances, molecular assays making use of amplification o...

journal_title：Journal of clinical laboratory analysis

authors： Auerbach AD,Verlander PC,Brown KE,Liu JM

更新日期：1997-01-01 00:00:00

abstract：OBJECTIVES:The objectives of this article are to investigate the serum lipid hydroperoxide (LOOH) levels and paraoxonase-1 (PON1) and arylesterase (ARE) activity in patients with lung, breast, and colorectal cancer. DESIGN AND METHODS:Serum PON1 and ARE activities and LOOH levels were measured in 110 patients with can...

journal_title：Journal of clinical laboratory analysis

authors： Balci H,Genc H,Papila C,Can G,Papila B,Yanardag H,Uzun H

更新日期：2012-05-01 00:00:00

abstract：BACKGROUND:The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high-quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM ...

journal_title：Journal of clinical laboratory analysis

authors： Sampedro A,Rodríguez-Granger J,Gómez C,Lara A,Gutierrez J,Otero A

更新日期：2013-11-01 00:00:00

abstract：:A sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for (anti-human T-cell leukemia virus type I) IgG (anti-HTLV-I IgG) in serum using recombinant gag p24(14-214) of HTLV-I is described. The recombinant gag p24(14-214) is soluble in the absence of detergents and allows the use of enzymes other ...

journal_title：Journal of clinical laboratory analysis

authors： Kohno T,Hirota K,Sakoda I,Yamasaki M,Yokoo Y,Ishikawa E

更新日期：1992-01-01 00:00:00

abstract：:Multidrug resistance (MDR) is a multifactorial phenomenon and the role of these proteins in generating the MDR phenotype is controversial. With this in mind, this review compiled the current data on the role of ABCB1, ABCC1, and LRP proteins in the prognosis of hematologic neoplasms and their influence on the choice o...

journal_title：Journal of clinical laboratory analysis

authors： de Moraes AC,Maranho CK,Rauber GS,Santos-Silva MC

更新日期：2013-01-01 00:00:00

abstract：:Candida dubliniensis is a recently described pathogenic species which shares many phenotypic features with Candida albicans and therefore, may be misidentified in microbiological laboratories. Because molecular methods can be onerous and unfeasible in routine mycological laboratories with restricted budgets such as th...

journal_title：Journal of clinical laboratory analysis

authors： de Loreto ES,Pozzatti P,Alves Scheid L,Santurio D,Morais Santurio J,Alves SH

更新日期：2008-01-01 00:00:00