Contribution of adiponectin polymorphisms to the risk of coronary artery disease in a North-African Tunisian population.

abstract：BACKGROUND:Metabolic syndrome (MetS) prevalence in rheumatoid arthritis (RA) patients is known to vary considerably across the world. This study aimed to determine the prevalence of MetS in RA patients from western Mexico and to analyze the interrelation of the MetS components with the clinical variables of RA. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： García-Chagollán M,Hernández-Martínez SE,Rojas-Romero AE,Muñoz-Valle JF,Sigala-Arellano R,Cerpa-Cruz S,Morales-Núñez JJ,Lomelí-Nieto JA,Macedo Ojeda G,Hernández-Bello J

更新日期：2020-11-24 00:00:00

abstract：:C-peptide is secreted in equimolar concentrations with insulin, and is often measured to assess pancreatic beta-cell function. C-peptide analysis is most often performed by radioimmunoassay (RIA) which has several disadvantages. We evaluated an automated, chemiluminescent immunoassay for C-peptide in terms of precisio...

journal_title：Journal of clinical laboratory analysis

authors： Hardy RW,Cohn M,Konrad RJ

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:Chronic hepatitis B (CHB) is one of the major infectious diseases in which CD4+ T helper (Th) cells play a crucial role. Th9 cells are a distinct subset of CD4+ Th cells with important physiological functions. OBJECTIVE:The study aimed to assess the potential involvement of Th9 cells in the inadequate immun...

journal_title：Journal of clinical laboratory analysis

authors： Cui M,Lv Y,Lu J,Zhang W,Duan Y,Huang Y,Yang L,Li M,Liu W,Liu D,Yan H

更新日期：2018-02-01 00:00:00

abstract：:Cholesterol in the diet can readily autoxidize and be absorbed and transported in plasma lipoproteins. Cholesterol oxides can also be endogenously produced in tissues via free-radical-induced reactions. Some cholesterol oxides, notably cholestane-3 beta, 5 alpha, 6 beta-triol and 25-hydroxycholesterol, have been shown...

journal_title：Journal of clinical laboratory analysis

authors： Peng SK,Hu B,Morin RJ

更新日期：1991-01-01 00:00:00

abstract：:Two methods for the extraction of RNA of vesicular stomatitis virus (VSV) Indiana1 and New Jersey and their simultaneous amplification by one-step polymerase chain reaction using reverse transcriptase were evaluated. A guanidine-thiocyanate-based RNA extraction (Qiagen RNeasy Mini Kit, Qiagen, Valencia, CA ) followed ...

journal_title：Journal of clinical laboratory analysis

authors： Arshed MJ,Magnuson RJ,Triantis J,Abubakar M,Van Campen H,Salman M

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:To study the role of toll-like receptor 4 (TLR4) and MicroRNA-155 (miR-155) in the peripheral blood mononuclear cell (PBMC)-mediated inflammation in coronary slow flow (CSF) and coronary arteriosclerosis. METHODS:Patients were divided into acute coronary syndrome (ACS), stable angina pectoris (SAP), CSF, an...

journal_title：Journal of clinical laboratory analysis

authors： Huang J,Yang Q,He L,Huang J

更新日期：2018-02-01 00:00:00

abstract：BACKGROUND:After hematopoietic stem cell transplantation (HSCT), early detection of engraftment would be of critical value for clinicians. The aim of this study was to identify faster parameters for engraftment. METHODS:We evaluated blood cell parameters including complete blood count (CBC), differential counts, and v...

journal_title：Journal of clinical laboratory analysis

authors： Yahng SA,Lee JW,Kim Y,Kim M,Oh EJ,Park YJ,Lee JW,Cho B,Han K

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:Fork head/winged helix transcription factor (Foxp3) plays a pivotal role in regulatory T (Treg) cells. The present study aimed to assess the association of Crohn's disease (CD) with Foxp3 polymorphisms and its colonic expression in Chinese patients. METHODS:The Foxp3 polymorphisms, rs3761547, rs2232365, rs2...

journal_title：Journal of clinical laboratory analysis

authors： Xia S,Zhang D,Zheng S,Wu C,Lin Q,Ying S,Shao X,Jiang Y

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:Aberrant levels of circulating microRNAs (miRNAs) are potential biomarkers in papillary thyroid carcinoma (PTC) diagnosis and therapy. The aim of this study was to evaluate serum exosomal miR-29a expression as a non-invasive biomarker for PTC diagnosis and prognosis. METHODS:Quantitative reverse transcripti...

journal_title：Journal of clinical laboratory analysis

authors： Wen Q,Wang Y,Li X,Jin X,Wang G

更新日期：2020-12-23 00:00:00

abstract：OBJECTIVE:To develop a reliable and specific method for rapid prenatal diagnosis of Trisomy 21 (Down syndrome). METHODS:We established a dual color competitive fluorescent Polymerase Chain Reaction (PCR) to measure the gene dosage of Down syndrome critical region (DSCR), a single copy sequence in chromosome 21. Anothe...

journal_title：Journal of clinical laboratory analysis

authors： Wang Y,Zhang X,Ling B,He C,Xia Q,Chen F,Miyamori I,Yang Z,Fan C

更新日期：2013-05-01 00:00:00

abstract：:Clinicians recognize and compensate for limitations in estimating the glomerular filtration rate (GFR) using serum creatinine (sCr) measurements by the use of timed collections and mathematical manipulations of sCr. These limitations stem from that fact that sCr is affected by nonrenal influences, including muscle mas...

journal_title：Journal of clinical laboratory analysis

authors： Massey D

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:A convenient biosensor for real-time measurement of biomarkers for in-field psychophysiological stress research and military operations is desirable. We evaluated a hand-held device for measuring salivary amylase as a stress marker in medical technicians undergoing combat casualty care training using two dif...

journal_title：Journal of clinical laboratory analysis

authors： Peng HT,Savage E,Vartanian O,Smith S,Rhind SG,Tenn C,Bjamason S

更新日期：2016-05-01 00:00:00

abstract：:Numerous detection methods for Helicobacter Pylori (H. pylori) have been developed with varying degrees of purported diagnostic utility. We have developed a rapid nonradioactive in situ hybridization (ISH) method for H. pylori detection in paraffin-embedded tissue and assessed its relative diagnostic performance by re...

journal_title：Journal of clinical laboratory analysis

authors： Barrett DM,Faigel DO,Metz DC,Montone K,Furth EE

更新日期：1997-01-01 00:00:00

abstract：BACKGROUND:Reference intervals are critical for interpreting test results of a clinical laboratory. The aim of this study was to establish local reference intervals of complete blood count for healthy preschoolers in China. METHODS:Three thousand eight hundred and twenty-one blood specimens from children aged 4 months...

journal_title：Journal of clinical laboratory analysis

authors： Wang GC,Li N,Niu C,Ma WB,Wang ZL,Guo H,Han LH

更新日期：2017-09-01 00:00:00

abstract：:Congenital heart disease (CHD) is a common cardiac defect found in infants and children. Despite advances in diagnosis and treatment, our understanding of the causative mechanism and etiology of CHD is limited. To determine the genetic etiology of CHD, we selected 11 consecutive short tandem-repeat polymorphic (STRP) ...

journal_title：Journal of clinical laboratory analysis

authors： Shi YR,Hsieh KS,Wu JY,Lee CC,Tsai CH,Yu MT,Chang JS,Tsai FJ

更新日期：2003-01-01 00:00:00

abstract：:In order to analyze the epitope structure of carcinoembryonic antigen (CEA) and the idiotype network system, seven anti-idiotypic monoclonal antibodies (anti-Id MoAbs) were generated from a BALB/c mouse immunized with anti-CEA MoAb P1-356, which recognized a synthetic peptide P1 of CEA, domain I. These MoAbs were divi...

journal_title：Journal of clinical laboratory analysis

authors： Tsujisaki M,Masuya J,Okada Y,Jinnohara T,Sasaki S,Yachi A,Imai K

更新日期：2002-01-01 00:00:00

abstract：:The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modifi...

journal_title：Journal of clinical laboratory analysis

authors： Gutiérrez J,Rodríguez MJ,De Ory F,Piédrola G,Maroto MC

更新日期：1999-01-01 00:00:00

abstract：BACKGROUND:Coronary artery disease (CAD) was the second leading cause of death for the past 3 years in Taiwan. The insulin-like growth factor (IGF) system is considered a new risk factor of CAD because investigations show that the levels and bioactivity of IGF-I and IGFBP-3 (where IGFBP is insulin-like growth factor-bi...

journal_title：Journal of clinical laboratory analysis

authors： Lin HL,Ueng KC,Wang HL,Chen TP,Yang SF,Chu SC,Hsieh YS

更新日期：2013-03-01 00:00:00

abstract：:Determination of free digoxin levels in patients treated with digoxin immune FAb has long been a problematic area in clinical laboratory testing. The older radioimmunoassays resulted in inaccurate and variable results due to the competition of the administered drug with the radioactively labelled forms. The 1995 Physi...

journal_title：Journal of clinical laboratory analysis

authors： Honda SA,Rios CN,Murakami L,Morita T,Scottolini AG,Bhagavan NV

更新日期：1995-01-01 00:00:00

abstract：OBJECTIVES:In this paper, a novel, sensitive and rapid method to simultaneously determine the free and total prostate-specific antigen (fPSA and tPSA) in serum by combining a time-resolved fluoroimmunoassay (TRFIA) and immunomagnetic separation was described. METHODS:The new approach uses magnetic particles as an immo...

journal_title：Journal of clinical laboratory analysis

authors： Zhou B,Zhang J,Lv Z,Fan J,Zhang Y,Huang B

更新日期：2017-11-01 00:00:00

abstract：:Radioimmunoassay (RIA) that involves purification of the analyte by organic solvent extraction is widely used. Although the extraction RIA is reliable when properly validated, it is time consuming and radioactive, we measure urinary total testosterone with a highly sensitive rapid and accurate time-resolved fluorescen...

journal_title：Journal of clinical laboratory analysis

authors： Bao S,Peng Y,Sheng S,Lin Q

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Definitive detection of hemoglobin (Hb) variants requires DNA sequencing. High-resolution melting (HRM) analysis of polymerase chain reaction (PCR) amplicons was applied to detect and discriminate among uncommon α-Hb variants found in Thailand. METHODS:Uncommon suspected α-Hb variants observed in Hb typing ...

journal_title：Journal of clinical laboratory analysis

authors： Yimniam W,Jindadamrongwech S

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:Kawasaki disease (KD) is an acute, self-limited vasculitis in infants and young children. Interleukin-10 (IL-10) is a potent cytokine that exerts pleiotropic effects on immunoregulation and inflammation. Elevated IL-10 serum levels have been reported in the KD patients. METHODS:In this study, we investigate...

journal_title：Journal of clinical laboratory analysis

authors： Lin YJ,Lan YC,Lai CH,Lin TH,Huang SM,Liao CC,Lin CW,Hung CH,Tien N,Liu X,Chien WK,Chen JH,Tsai FJ

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:Dried blood spots (DBS) are an important form of bio-sampling and valuable approach for storing blood samples for genetic studies. This has necessitated in developing an effective protocol to isolate genomic DNA (gDNA) from DBS samples.In this study, we have elucidated a dependable and non-hazardous "single ...

journal_title：Journal of clinical laboratory analysis

authors： Shaik M,Shivanna DK,Kamate M,Ab V,Tp KV

更新日期：2016-11-01 00:00:00

abstract：:Circulating bilirubin is thought to function as a physiological antioxidant. One of the decomposition products of this process is the biopyrrins, which include two regioisomers: biotripyrrin-a (1,14,15,17-tetrahydro-2,7,13-trimethyl-1,14-deoxy-3-vinyl-16H-tripyrrin-8,12-dipropionic acid) and biotripyrrin-b (1,14,15,17...

journal_title：Journal of clinical laboratory analysis

authors： Ihara H,Matsumoto T,Morita Y,Hirano A,Okada M,Hashizume N,Shioji I,Yoshimura H

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:Delta neutrophil index (DNI) is a new inflammatory marker and the present study aimed to evaluate the predictive value of the DNI for the presence of a perforation in elderly with acute appendicitis. METHODS:This retrospective observational study was conducted on 108 consecutive elderly patients (≥65 years ...

journal_title：Journal of clinical laboratory analysis

authors： Shin DH,Cho YS,Kim YS,Ahn HC,Oh YT,Park SO,Won MH,Cho JH,Kim YM,Seo JY,Lee YH

更新日期：2018-01-01 00:00:00

abstract：BACKGROUND:Acute aortic dissection (AOD) is associated with high mortality and early diagnosis and treatment are essential. Ischemia-modified albumin (IMA) is a marker of myocardial ischemia whereas cardiac enzymes are released when myocardial necrosis occurs. We investigated, for the first time, whether IMA increases ...

journal_title：Journal of clinical laboratory analysis

authors： Sbarouni E,Georgiadou P,Marathias A,Panagiotakos D,Geroulanos S,Voudris V

更新日期：2010-01-01 00:00:00

abstract：:We studied a new proteinase K assay method for human serum mitochondrial aspartate aminotransferase. We found that proteinase K showed no inactivation of human mitochondrial aspartate aminotransferase isoenzyme and complete inactivation of cytosolic aspartate aminotransferase. Previous studies have shown that selectiv...

journal_title：Journal of clinical laboratory analysis

authors： Watazu Y,Uji Y,Okabe H,Shirahase Y,Kaneda N,Karmen A

更新日期：1993-01-01 00:00:00

abstract：:Hemoglobin F (HbF) is an effective inhibitor of HbS polymerization. Hydroxyurea (HU) is used to increase HbF synthesis and improve the clinical course of sickle cell disease (SCD) patients. We studied a series of laboratory parameters concerning HbF production and reticulocyte response, and compared data between two g...

journal_title：Journal of clinical laboratory analysis

authors： Borba R,Lima CS,Grotto HZ

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND:Little is known about the involvement of herpes simplex virus (HSV) or Mycobacterium tuberculosis (MTB) as potentially curable causes of central nervous system (CNS) infections in sub-Saharan Africa. OBJECTIVE:In this study, we developed a PCR assay dedicated to simultaneous testing of HSV1/HSV2 and MTB in ...

journal_title：Journal of clinical laboratory analysis

authors： Zida S,Kolia-Diafouka P,Kania D,Sotto A,Foulongne V,Bolloré K,Ouangraoua S,Méda N,Carrère-Kremer S,Van de Perre P,Tuaillon E

更新日期：2019-03-01 00:00:00