Abstract：
:Two methods for the extraction of RNA of vesicular stomatitis virus (VSV) Indiana1 and New Jersey and their simultaneous amplification by one-step polymerase chain reaction using reverse transcriptase were evaluated. A guanidine-thiocyanate-based RNA extraction (Qiagen RNeasy Mini Kit, Qiagen, Valencia, CA ) followed by column-based purification coupled with one-step RT-PCR proved to be a simple, safe, practicable, and reliable tool for rapid, highly sensitive, and specific differential diagnosis of both types of VSV in cell lysate and spiked tissue samples as compared with the tri-phasic extraction method (Tri-reagent method). When RNA was extracted either from VSV cell culture stock or from VSV spiked bovine lymph nodes by using Qiagen RNeasy Mini Kit, the detection limit in the multiplex RT-PCR was as low as 0.505 to 2.84 TCID(50) for VSV-IND and VSV-NJ, respectively. The multiplex RT-PCR consistently detected VSV-IND and NJ RNA in as little as 0.1-1.0 fg of total RNA from spiked BHK-21 cell suspension when Qiagen RNeasy mini kit was used. The multiplex RT-PCR assay was capable of detecting both types of VSV in a one-step reaction tube. The minimum sensitivity of this assay in various experiments was 0.1683 TCID(50) (IND), 0.0946 TCID(50) (NJ), and 0.057 fg (IND and NJ) per 2 µl PCR sample, which is significantly more sensitive than reported previously (0.28-2.8 TCID50/1 µl). So the present study improved the sensitivity of previously reported multiplex RT-PCR for the detection and differentiation of VSV-IND and VSV-NJ in a single assay.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Arshed MJ,Magnuson RJ,Triantis J,Abubakar M,Van Campen H,Salman Mdoi
10.1002/jcla.20439subject
Has Abstractpub_date
2011-01-01 00:00:00pages
95-9issue
2eissn
0887-8013issn
1098-2825journal_volume
25pub_type
杂志文章abstract：:This study was designed to measure the correlation between left ventricular (LV) remodeling 3 months after successful reperfusion therapy, and the levels of serum N-terminal pro-brain natriuretic peptide (NT-proBNP) and high-sensitivity C-reactive protein (hs-CRP) at 3 days after acute myocardial infarction (AMI), and...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20094
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abstract：OBJECTIVE:This study was to investigate the changes of antifungal susceptibilities caused by the phenotypic switching in patients with vulvovaginal candidiasis (VVC). METHODS:229 women were enrolled in this study. The vaginal smears of these patients were collected and gram stained for fungal microscopic observation. ...
journal_title：Journal of clinical laboratory analysis
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doi：10.1002/jcla.22644
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abstract：BACKGROUND:Functions, morphology, distributions, and index of the circulating cells are the most useful parameters that indicate various inflammatory and toxic conditions. The aim of this study was to investigate the clinical significance of these parameters in patients diagnosed with (synthetic) cannabis use disorder....
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22131
更新日期：2017-11-01 00:00:00
abstract：:The experimental and clinical effectiveness of nasal salmon calcitonin (SCT) for treatment of osteoporosis in humans has been well established, but none is known yet about the pharmacokinetic property in relation to therapeutic efficacy, especially when used in a therapeutic dose range. This preclinical study was desi...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/(sici)1098-2825(1997)11:3<129::aid-jcla2>3
更新日期：1997-01-01 00:00:00
abstract：:Multidrug resistance (MDR) is a multifactorial phenomenon and the role of these proteins in generating the MDR phenotype is controversial. With this in mind, this review compiled the current data on the role of ABCB1, ABCC1, and LRP proteins in the prognosis of hematologic neoplasms and their influence on the choice o...
journal_title：Journal of clinical laboratory analysis
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abstract：BACKGROUND:Adiponectin, an adipocyte-derived protein, is known to play a key role in the processes leading to atherosclerosis and coronary artery disease (CAD) through its anti-atherogenic, anti-inflammatory, antioxidative, and anti-apoptotic properties. In the current study, we have studied the association of two sing...
journal_title：Journal of clinical laboratory analysis
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abstract：BACKGROUND:The non-invasive diagnostic approach for early detection of endometrial cancer (EC) remains limited. To date, human epididymis protein 4 (HE4) has been intensively studied but its diagnostic is controversial in EC. DJ-1 is an oncoprotein secreted by cancer cells, recently identified as a potential diagnostic...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22223
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abstract：:Didemnin A was conjugated at the amino terminus of the N-methylleucine residue, via the linkers N-succinimidyl-3-(2-pyridyldithio)-propionate and trans-1,4-maleimidomethyl-cyclohexane carboxylic acid, to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA). The didemnin-KLH conjugates were used to hyperimmun...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860060307
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abstract：:The detection of hepatitis C virus (HCV) RNA by nucleic acid amplification techniques is the method of choice to differentiate between ongoing and past infection, and can be used to monitor the course of HCV infection. In this study, we evaluated the performance characteristics of a newly developed transcription-media...
journal_title：Journal of clinical laboratory analysis
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abstract：:We developed enzyme immunoassays for human anti-Pichia pastoris components (PPC) IgG and anti-PPC IgE antibody titers. Anti-PPC IgG antibody assay were performed using antigen-coated plate and anti-human IgG peroxidase conjugate. The intra- and interassay coefficients of variation (CV) of anti-PPC IgG antibody were 1....
journal_title：Journal of clinical laboratory analysis
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doi：
更新日期：1997-01-01 00:00:00
abstract：:The distribution of the major VL subgroups (V lambda I, V lambda II, V lambda III, V lambda IV, V lambda VI, and V lambda VIII) among lambda-type immunoglobulins (Igs) in normal serum was determined by a sandwich enzyme-linked immunosorbent assay (ELISA) using a panel of murine anti-human V lambda-subgroup-specific mo...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860080103
更新日期：1994-01-01 00:00:00
abstract：:The incidence of aneuploidy was determined by flow cytometric analysis in paraffin-embedded tumor samples of 125 patients with non-Hodgkin lymphoma (NHL). There were 48 low-grade (LG) and 77 high-grade (HG) tumors. Aneuploidy was found in 34 (27%) NHL, 15 (31%) LG, and 19 (25%) HG tumors. The analysis of patient survi...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860090602
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND:Endometriosis (EMS) is a prevalent gynecological condition characterized by the growth of endometrial tissue outside the uterine cavity. This study aimed to clarify the targeted therapeutic effect of sunitinib in an endometriosis in vitro experiment. METHODS:Primary culture of ectopic endometrial cells and ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23482
更新日期：2020-11-01 00:00:00
abstract：BACKGROUND:This study was performed to investigate the epidemiology of enterovirus (EV) infections in children at a single hospital during recent 5 years. This study was preformed from December 2008 to November 2013. METHODS:We subjected 1,067 cerebrospinal fluid (CSF) specimens from 1,053 patients and 678 stool speci...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21830
更新日期：2016-03-01 00:00:00
abstract：:Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections. The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis identification. fbl gene, encoding a fibrinogen-binding adhesin, was exploited and assessed ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20352
更新日期：2010-01-01 00:00:00
abstract：:The technique that allows for in situ detection of PCR-amplified DNA and cDNA has improved remarkably quickly over the past 5 years. As of this writing, one can expect good results after a relatively quick "learning curve." The technique demands some skill in working with glass slides and cells/tissues. Thus, I recomm...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章,评审
doi：10.1002/(SICI)1098-2825(1996)10:6<335::AID-JCLA5>3
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND:The aim of our study was to assess two tumor markers, carbohydrate antigen 724 (CA724) and neuron-specific enolase (NSE), in 20 healthy individuals. Then, we determined their average intra- and inter-individual biological variations (CVI and CVG ). METHODS:Blood samples were taken at weekly intervals for fi...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22327
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Cellular and brain metabolism of dopamine can be correlated with a number of neurodegenerative disorders, our study was to explore a simple and efficient method to detect dopamine in real samples. METHODS:A new quantum dots (CdTe QDs) could be prepared using the hydrothermal method, the electrochemical bios...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22320
更新日期：2018-03-01 00:00:00
abstract：BACKGROUND:The many techniques used to diagnose the Factor V Leiden (FVL) mutation, the most common hereditary hypercoagulation disorder in Eurasians, and the most frequently requested genetic test reflect the evolving strategies in protein and DNA diagnosis. METHODS:Here, molecular methods to diagnose the FVL mutatio...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20470
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:This study aimed to investigate the relationship between HbA1c and RBC aggregation in type 2 diabetes mellitus (T2DM) patients by analysis of data from routine clinical tests and from in vitro experiments. METHODS:A total of 2,111 inpatients with type 2 diabetes were selected and among them, 364 patients (G...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21784
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journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21866
更新日期：2016-09-01 00:00:00
abstract：:A sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for (anti-human T-cell leukemia virus type I) IgG (anti-HTLV-I IgG) in serum using recombinant gag p24(14-214) of HTLV-I is described. The recombinant gag p24(14-214) is soluble in the absence of detergents and allows the use of enzymes other ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860060509
更新日期：1992-01-01 00:00:00
abstract：:It is well established that the targeted receptor for ciguatoxin (CTX) in mammalian tissues is the sodium channel, affecting the influx of sodium into cells and altering the action potential and function of the cell. Since the syntheses of fragments of CTX has become available, our focus has been on the receptor funct...
journal_title：Journal of clinical laboratory analysis
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doi：10.1002/jcla.20114
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abstract：BACKGROUND:Today, the genetic and genomic research entered in a new era of high-throughput genotyping technology. However, mutagenic polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is still a choice of genotyping method in molecular epidemiological research. It has been extensively used fo...
journal_title：Journal of clinical laboratory analysis
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更新日期：2016-03-01 00:00:00
abstract：:Determination of free digoxin levels in patients treated with digoxin immune FAb has long been a problematic area in clinical laboratory testing. The older radioimmunoassays resulted in inaccurate and variable results due to the competition of the administered drug with the radioactively labelled forms. The 1995 Physi...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860090612
更新日期：1995-01-01 00:00:00
abstract：OBJECTIVES:Existing evidence suggests that impaired vitamin D metabolism contribute to the development of atherosclerosis. Aortic intima-media thickness (IMT) is an earlier marker than carotid IMT of preclinical atherosclerosis. However, there is a lack of researches on direct investigation of relevance between serum 2...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21770
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND:Multiple organ dysfunction syndromes (MODS) is reported as a leading cause of mortality in intensive care units. Recently, continuous blood purification (CBP) has been mostly applied for MODS treatment. Thus, the purpose of this study was to investigate the effects of CBP on plasma phospholipid level in pati...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22411
更新日期：2018-07-01 00:00:00
abstract：:The shortcomings of current methods of basophil enumeration detract from the clinical value of the basophil count. Moreover, sophisticated and costly techniques of automated basophil counting hardly can be validated for lack of a suitable reference method. We investigated whether a flow cytometric technique using doub...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2825(1996)10:4<177::AID-JCLA2>3
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abstract：BACKGROUND:Although a diagnosis of infectious diseases is essential for timely treatment, the performance of diagnostic tests has been hardly evaluated due to variable results that are influenced by multiple factors in different conditions. In the present study, the performance of the Alinity i system, which is a newly...
journal_title：Journal of clinical laboratory analysis
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doi：10.1002/jcla.23671
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abstract：BACKGROUND:It has been identified that incidence of infertility was about 20% among couples worldwide, about 50% caused by male elements. However, conventional semen laboratory detections could not handle clinical needs, which led to more comprehensive parameters for male fertility evaluation. We aimed to investigate t...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23548
更新日期：2020-09-14 00:00:00
