Rapid and sensitive headspace gas chromatography-mass spectrometry method for the analysis of ethanol in the whole blood.

abstract：:The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a consi...

journal_title：Journal of clinical laboratory analysis

authors： Noguera NI,Tallano CE,Bragós IM,Milani AC

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:Essential trace elements are vital to human health. In this study, our aim was to establish reference intervals of and to evaluate relationships among Ca, Fe, Cu, Mg, and Zn for children. METHODS:We collected blood samples of 3210 children aged 0-14 years from Lu'an, China, and concentrations of the above e...

journal_title：Journal of clinical laboratory analysis

authors： Zhai R,Zhang M,Liu J,Guang H,Li B,Chen D,Zhang S

更新日期：2017-03-01 00:00:00

abstract：BACKGROUND:Previous studies have demonstrated that dysfunctional regulatory T cells (Tregs) may be associated with Graves' disease (GD). In this study, we evaluated four serum Treg-associated miRNAs (miR-210, miR-182, miR-155, and miR-146a) expressions and assessed the potential of serum miRNAs as biomarkers of GD. ME...

journal_title：Journal of clinical laboratory analysis

authors： Zheng L,Zhuang C,Wang X,Ming L

更新日期：2018-02-01 00:00:00

abstract：BACKGROUND:Wolfram's syndrome (WFS) is a hereditary (autosomal recessive) neurodegenerative disorder. The clinical features are related to diabetes insipidus, diabetes mellitus, optic atrophy, and deafness (DIDMOAD) with other variable clinical manifestations. Pathogenic variants in the WFS1 gene, encoding wolframin, a...

journal_title：Journal of clinical laboratory analysis

authors： Torkamandi S,Rezaei S,Mirfakhraie R,Bayat S,Piltan S,Gholami M

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND:The non-invasive diagnostic approach for early detection of endometrial cancer (EC) remains limited. To date, human epididymis protein 4 (HE4) has been intensively studied but its diagnostic is controversial in EC. DJ-1 is an oncoprotein secreted by cancer cells, recently identified as a potential diagnostic...

journal_title：Journal of clinical laboratory analysis

authors： Benati M,Montagnana M,Danese E,Paviati E,Giudici S,Ruzzenente O,Franchi M,Lippi G

更新日期：2018-01-01 00:00:00

abstract：BACKGROUND:Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray ...

journal_title：Journal of clinical laboratory analysis

authors： Ciepiela O,Kotuła I,Kierat S,Sieczkowska S,Podsiadłowska A,Jenczelewska A,Księżarczyk K,Demkow U

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:The prevalence of diabetes is increasing in China. Glucose control is very important in diabetic patients. The aim of this study was to compare the accuracy of five glucose meters used in Chinese hospitals with a reference method, in the absence and presence of various factors that may interfere with the met...

journal_title：Journal of clinical laboratory analysis

authors： Lv H,Zhang GJ,Kang XX,Yuan H,Lv YW,Wang WW,Randall R

更新日期：2013-09-01 00:00:00

abstract：BACKGROUND:Automated cell counters have become more and more sophisticated with passing years. The numerical and graphic data both provide useful clues for suspecting a diagnosis especially when the workload is very high. AIM:We present our experience of useful information provided by graphic displays of an automated ...

journal_title：Journal of clinical laboratory analysis

authors： Gupta M,Chauhan K,Singhvi T,Kumari M,Grover RK

更新日期：2018-06-01 00:00:00

abstract：BACKGROUND:Circular RNAs (circRNAs) are emerging noncoding RNAs that are involved in many biological processes and diseases. The expression profile of circRNAs in preterm neonates with bronchopulmonary dysplasia (BPD) remains unresolved. METHODS:In BPD infants, peripheral venous blood was drawn and circRNAs were extra...

journal_title：Journal of clinical laboratory analysis

authors： Mao X,Guo Y,Qiu J,Zhao L,Xu J,Yin J,Lu K,Zhang M,Cheng R

更新日期：2020-07-01 00:00:00

abstract：BACKGROUND:So far, there has been no tool to estimate activity at diagnosis and predict all-cause mortality in patients with ANCA-associated vasculitis (AAV). Hence, we determined the initial predictors of them in patients with AAV. METHODS:We retrospectively reviewed the medical records of 182 patients with AAV. Seve...

journal_title：Journal of clinical laboratory analysis

authors： Kim MK,Choi H,Kim JY,Song JJ,Park YB,Lee SW

更新日期：2020-01-01 00:00:00

abstract：BACKGROUND:The aim of this study was to investigate the differential plasma levels of lipocalin 2 (LCN2) and its complex with MMP-9 (where MMP is matrix metalloproteinase) before and after antibiotic treatment in hospitalized adult patients with community-acquired pneumonia (CAP). METHOD:Plasma LCN2 and LCN2/MMP-9 com...

journal_title：Journal of clinical laboratory analysis

authors： Yeh YH,Chang JL,Hsiao PC,Tsao SM,Lin CH,Kao SJ,Chou MC,Yang SF,Chien MH

更新日期：2013-07-01 00:00:00

abstract：BACKGROUND:Coronary heart disease (CHD) is primarily caused by atherosclerosis of coronary arteries. It is largely an inflammatory disease of the vascular wall. The inflammation is related to DNA methylation. Angiopoietin-like protein 2 (ANGPTL2) has various functions in several chronic inflammatory diseases. Macrophag...

journal_title：Journal of clinical laboratory analysis

authors： Zhou J,Chen L,Yang X,Huang X,Wang Z,Peng P,Lian J

更新日期：2019-03-01 00:00:00

abstract：:Antibodies to neutrophil cytoplasmic antigens (ANCA) targeted toward granule enzymes have been recognized as a valuable diagnostic tool in the detection of Wegener's granulomatosis and systemic vasculitides. However, the most commonly used method of detection, the indirect immunofluorescence assay, is prone to false-p...

journal_title：Journal of clinical laboratory analysis

authors： Streicher J,Fabian B,Herkner K,Pointner H,Bayer PM

更新日期：1998-01-01 00:00:00

abstract：BACKGROUND:This study was aimed to investigate whether measurement of free testosterone and cortisol in saliva is a reliable alternative to their assessment in serum for monitoring physical fitness in professional athletes. METHODS:We studied 25 members of the soccer team Parma F.C., playing in Italian major football ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Dipalo M,Buonocore R,Gnocchi C,Aloe R,Delsignore R

更新日期：2016-09-01 00:00:00

abstract：:Second- and higher-order degradation reactions sometimes cannot be approximated with linear or exponential relationships and need to be appropriately modeled. Events above the COULTER HmX Analyzer white blood cell (WBC) counting threshold were recorded for the HmX PAK reagent system stored at five elevated temperature...

journal_title：Journal of clinical laboratory analysis

authors： Magari RT,Murphy KP,Fernandez T

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:Neuron-specific enolase (NSE) is a recognized biomarker for the assessment of cerebral injury in neurological disorders. This study aims to report a definitive assessment of the biological variation (BV) components of this biomarker, including within-subject BV (CVI), between-subject BV (CVG), index of indiv...

journal_title：Journal of clinical laboratory analysis

authors： Matyar S,Goruroglu Ozturk O,Ziyanoglu Karacor E,Yuzbasioglu Ariyurek S,Sahin G,Kibar F,Yaman A,Inal T

更新日期：2016-11-01 00:00:00

abstract：AIMS:Essential hypertension (EH) is a high prevalence disease facing a public health challenge. People were little known about the genetics of diagnosing the cause of EH. Circular RNAs that have a continuous cycle of covalent closure, without affected by RNA exonuclease, and are more stable and hard to degrade may invo...

journal_title：Journal of clinical laboratory analysis

authors： Bao X,He X,Zheng S,Sun J,Luo Y,Tan R,Zhao J,Zhong F,Zhang L

更新日期：2019-05-01 00:00:00

abstract：BACKGROUND:Diverse and circumstantial evidence suggests that schizophrenia is a neurodevelopmental disorder. Genes contributing to neurodevelopment may be potential candidates for schizophrenia. The human SOX11 gene is a member of the developmentally essential SOX (Sry-related HMG box) transcription factor gene family ...

journal_title：Journal of clinical laboratory analysis

authors： Sun CP,Sun D,Luan ZL,Dai X,Bie X,Ming WH,Sun XW,Huo XX,Lu TL,Zhang D

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND:Low concentration C-reactive protein (CRP) has favorable prognostic significance in patients with cardiovascular risks. METHODS:We compared the wr-CRP method with the hs-CRP method both on Roche Cobas c702 analyzer for the determination of low CRP concentration (<20 mg/L) including 200 patients treated in C...

journal_title：Journal of clinical laboratory analysis

authors： Li R,Xue Y,Wang T,Gong L,Peng P,Xiong P,Dai M,Shao T,Hu Y,Ye X

更新日期：2019-10-01 00:00:00

abstract：BACKGROUND:Recently, several studies have investigated the relationship between Pre-miR-27a rs895819 polymorphism and risk of various cancers. However, the relationship between rs895819 and diffuse large B-cell lymphoma (DLBCL) has not been well known. METHODS:In this study, we conducted a case-control study to explor...

journal_title：Journal of clinical laboratory analysis

authors： Tang W,Xu H,Ma D,Ma R,Wu J,Yu X,Feng J,Liu Q

更新日期：2020-03-01 00:00:00

abstract：BACKGROUND:Endometriosis (EMS) is a prevalent gynecological condition characterized by the growth of endometrial tissue outside the uterine cavity. This study aimed to clarify the targeted therapeutic effect of sunitinib in an endometriosis in vitro experiment. METHODS:Primary culture of ectopic endometrial cells and ...

journal_title：Journal of clinical laboratory analysis

authors： Li J,Abudula M,Fan X,Wang F,Chen Y,Liu L

更新日期：2020-11-01 00:00:00

abstract：:Type IV collagen is a major component released from the glomerular and tubular basement membranes. To investigate the alteration of renal type IV collagen turnover in early stage diabetic nephropathy, urinary type IV collagen was measured by a highly sensitive one-step sandwich enzyme immunoassay (EIA). Urinary sample...

journal_title：Journal of clinical laboratory analysis

authors： Iijima T,Suzuki S,Sekizuka K,Hishiki T,Yagame M,Jinde K,Saotome N,Suzuki D,Sakai H,Tomino Y

更新日期：1998-01-01 00:00:00

abstract：BACKGROUND:Toxocariasis is the clinical term that is applied to infection in the human host with Toxocara species larvae. Serological tests are important tools for the diagnosis of toxocariasis. The aim of this study was to evaluate the excretory-secretory (ES) antigens of T. cati larvae using enzyme-linked immunosorbe...

journal_title：Journal of clinical laboratory analysis

authors： Zibaei M,Sadjjadi SM,Sarkari B,Uga S

更新日期：2016-05-01 00:00:00

abstract：:Novel molecular approaches have recently become available allowing improved major histocompatibility complex (MHC) matching of potential allogeneic bone marrow donors and recipients. Current cellular and serological assays are hindered by aberrant cell populations and limited reagents which only detect an individuals'...

journal_title：Journal of clinical laboratory analysis

authors： Rubocki RJ,Wisecarver JL,Hook DD,Cox SM,Beisel KW

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:The pulmonary surfactant especially lipids in amniotic fluid can reflect the development stage of fetal lung maturity (FLM). However, the conventional lecithin/sphingomyelin (L/S) ratio method by thin layer chromatography (TLC) is insufficient and inconvenient for FLM prediction in clinical practice. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Cao Z,Liu J,Xie X,Zhan S,Song W,Wu S,Sun Z,Dong Y,Tang G,Liu Y,Li L,Shen M,Zhai Y,Zou J,Liu X

更新日期：2020-04-01 00:00:00

abstract：BACKGROUND:Acute aortic dissection (AOD) is associated with high mortality and early diagnosis and treatment are essential. Ischemia-modified albumin (IMA) is a marker of myocardial ischemia whereas cardiac enzymes are released when myocardial necrosis occurs. We investigated, for the first time, whether IMA increases ...

journal_title：Journal of clinical laboratory analysis

authors： Sbarouni E,Georgiadou P,Marathias A,Panagiotakos D,Geroulanos S,Voudris V

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:There is a high prevalence of Hepatitis B virus (HBV) infection in China. The aim of this article is to reveal the HBV prevalence and its outcome in southern China by analyzing the HBV serum markers and their relationship to HBV genotype. METHODS:HBV serum markers, HBV-DNA and Alanine aminotransferase (ALT)...

journal_title：Journal of clinical laboratory analysis

authors： Lin S,Liu C,Shang H,Chen H,Yang B,Chen J,Chen Y,Chen D,Ou Q

更新日期：2013-03-01 00:00:00

abstract：BACKGROUND:A-kinase-interacting protein 1 (AKIP1) has been reported as an oncogenetic factor in multiple cancers; however, no study has reported its role in non-small cell lung cancer (NSCLC) yet. This study aimed to evaluate the expression of AKIP1, and its correlation with tumor characteristics as well as prognosis i...

journal_title：Journal of clinical laboratory analysis

authors： Liu Y,Tian J,Qin D,Liu J,Xie Y

更新日期：2020-04-01 00:00:00

abstract：:The two serum proteins of the complement cascade in the highest concentrations, C3 and C4, respond to various conditions in much the same manner as do other positive acute-phase proteins. A major difference is that they are relatively sluggish in response to cytokine drive, requiring several days rather than hours to ...

journal_title：Journal of clinical laboratory analysis

authors： Ritchie RF,Palomaki GE,Neveux LM,Navolotskaia O,Ledue TB,Craig WY

更新日期：2004-01-01 00:00:00

abstract：:United States Food and Drug Administration (FDA) in 2010 approved the use of immunosuppressant drug everolimus, which requires therapeutic drug monitoring in whole blood. Taking advantage of structural similarity between sirolimus and everolimus we attempted to rapidly estimate everolimus concentration from apparent s...

journal_title：Journal of clinical laboratory analysis

authors： Dasgupta A,Moreno V,Balark S,Smith A,Sonilal M,Tejpal N,Van Buren CT

更新日期：2011-01-01 00:00:00