Rapid and sensitive headspace gas chromatography-mass spectrometry method for the analysis of ethanol in the whole blood.


BACKGROUND:For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n-propanol as internal standard. GC was performed in isothermal mode with a GC run-time of 5.0 min. METHODS:The quantification was performed using selected ions monitoring mode adopting a quantitative ion and qualifier ion for ethanol and the internal standard. RESULTS:The method was linear (r(2) = 0.999, in the concentration range of 39.5-1,262.9 μg/ml), specific, sensitive (limit of quantification and limit of detection of 39.5 and 0.4 μg/ml, respectively), and robust. A slightly modified method was also developed for the quantification of 50 commonly abused drunken in blood. The method used an isothermal GC program with a run-time of 5.0 min. The quantification was performed using selected ions monitoring mode and integrating the area under the peak using n-propanol as an internal standard. The method was linear 40-1,263 μg/ml and sensitive. CONCLUSIONS:The method was proved superior in speed and selectivity to previously reported methods and was successfully applied to the pharmacokinetic study of ethanol.


J Clin Lab Anal


Xiao HT,He L,Tong RS,Yu JY,Chen L,Zou J,Li JQ,Bian Y,Zhang Y




Has Abstract


2014-09-01 00:00:00












  • Modified salting-out method for DNA isolation from newborn cord blood nucleated cells.

    abstract::The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a consi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Noguera NI,Tallano CE,Bragós IM,Milani AC

    更新日期:2000-01-01 00:00:00

  • Reference Intervals of and Relationships among Essential Trace Elements in Whole Blood of Children Aged 0-14 years.

    abstract:BACKGROUND:Essential trace elements are vital to human health. In this study, our aim was to establish reference intervals of and to evaluate relationships among Ca, Fe, Cu, Mg, and Zn for children. METHODS:We collected blood samples of 3210 children aged 0-14 years from Lu'an, China, and concentrations of the above e...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Zhai R,Zhang M,Liu J,Guang H,Li B,Chen D,Zhang S

    更新日期:2017-03-01 00:00:00

  • Serum miR-146a, miR-155, and miR-210 as potential markers of Graves' disease.

    abstract:BACKGROUND:Previous studies have demonstrated that dysfunctional regulatory T cells (Tregs) may be associated with Graves' disease (GD). In this study, we evaluated four serum Treg-associated miRNAs (miR-210, miR-182, miR-155, and miR-146a) expressions and assessed the potential of serum miRNAs as biomarkers of GD. ME...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Zheng L,Zhuang C,Wang X,Ming L

    更新日期:2018-02-01 00:00:00

  • A homozygous missense mutation of WFS1 gene causes Wolfram's syndrome without hearing loss in an Iranian family (a report of clinical heterogeneity).

    abstract:BACKGROUND:Wolfram's syndrome (WFS) is a hereditary (autosomal recessive) neurodegenerative disorder. The clinical features are related to diabetes insipidus, diabetes mellitus, optic atrophy, and deafness (DIDMOAD) with other variable clinical manifestations. Pathogenic variants in the WFS1 gene, encoding wolframin, a...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Torkamandi S,Rezaei S,Mirfakhraie R,Bayat S,Piltan S,Gholami M

    更新日期:2020-08-01 00:00:00

  • The clinical significance of DJ-1 and HE4 in patients with endometrial cancer.

    abstract:BACKGROUND:The non-invasive diagnostic approach for early detection of endometrial cancer (EC) remains limited. To date, human epididymis protein 4 (HE4) has been intensively studied but its diagnostic is controversial in EC. DJ-1 is an oncoprotein secreted by cancer cells, recently identified as a potential diagnostic...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Benati M,Montagnana M,Danese E,Paviati E,Giudici S,Ruzzenente O,Franchi M,Lippi G

    更新日期:2018-01-01 00:00:00

  • A Comparison of Mindray BC-6800, Sysmex XN-2000, and Beckman Coulter LH750 Automated Hematology Analyzers: A Pediatric Study.

    abstract:BACKGROUND:Modern automated laboratory hematology analyzers allow the measurement of over 30 different hematological parameters useful in the diagnostic and clinical interpretation of patient symptoms. They use different methods to measure the same parameters. Thus, a comparison of complete blood count made by Mindray ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Ciepiela O,Kotuła I,Kierat S,Sieczkowska S,Podsiadłowska A,Jenczelewska A,Księżarczyk K,Demkow U

    更新日期:2016-11-01 00:00:00

  • Factors interfering with the accuracy of five blood glucose meters used in Chinese hospitals.

    abstract:BACKGROUND:The prevalence of diabetes is increasing in China. Glucose control is very important in diabetic patients. The aim of this study was to compare the accuracy of five glucose meters used in Chinese hospitals with a reference method, in the absence and presence of various factors that may interfere with the met...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Lv H,Zhang GJ,Kang XX,Yuan H,Lv YW,Wang WW,Randall R

    更新日期:2013-09-01 00:00:00

  • Useful information provided by graphic displays of automated cell counter in hematological malignancies.

    abstract:BACKGROUND:Automated cell counters have become more and more sophisticated with passing years. The numerical and graphic data both provide useful clues for suspecting a diagnosis especially when the workload is very high. AIM:We present our experience of useful information provided by graphic displays of an automated ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Gupta M,Chauhan K,Singhvi T,Kumari M,Grover RK

    更新日期:2018-06-01 00:00:00

  • Next-generation sequencing to investigate circular RNA profiles in the peripheral blood of preterm neonates with bronchopulmonary dysplasia.

    abstract:BACKGROUND:Circular RNAs (circRNAs) are emerging noncoding RNAs that are involved in many biological processes and diseases. The expression profile of circRNAs in preterm neonates with bronchopulmonary dysplasia (BPD) remains unresolved. METHODS:In BPD infants, peripheral venous blood was drawn and circRNAs were extra...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Mao X,Guo Y,Qiu J,Zhao L,Xu J,Yin J,Lu K,Zhang M,Cheng R

    更新日期:2020-07-01 00:00:00

  • Multivariable index for assessing the activity and predicting all-cause mortality in antineutrophil cytoplasmic antibody-associated vasculitis.

    abstract:BACKGROUND:So far, there has been no tool to estimate activity at diagnosis and predict all-cause mortality in patients with ANCA-associated vasculitis (AAV). Hence, we determined the initial predictors of them in patients with AAV. METHODS:We retrospectively reviewed the medical records of 182 patients with AAV. Seve...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Kim MK,Choi H,Kim JY,Song JJ,Park YB,Lee SW

    更新日期:2020-01-01 00:00:00

  • Circulating level of lipocalin 2 as a predictor of severity in patients with community-acquired pneumonia.

    abstract:BACKGROUND:The aim of this study was to investigate the differential plasma levels of lipocalin 2 (LCN2) and its complex with MMP-9 (where MMP is matrix metalloproteinase) before and after antibiotic treatment in hospitalized adult patients with community-acquired pneumonia (CAP). METHOD:Plasma LCN2 and LCN2/MMP-9 com...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Yeh YH,Chang JL,Hsiao PC,Tsao SM,Lin CH,Kao SJ,Chou MC,Yang SF,Chien MH

    更新日期:2013-07-01 00:00:00

  • Preliminary study of the relationship between promoter methylation of the ANGPTL2 gene and coronary heart disease.

    abstract:BACKGROUND:Coronary heart disease (CHD) is primarily caused by atherosclerosis of coronary arteries. It is largely an inflammatory disease of the vascular wall. The inflammation is related to DNA methylation. Angiopoietin-like protein 2 (ANGPTL2) has various functions in several chronic inflammatory diseases. Macrophag...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Zhou J,Chen L,Yang X,Huang X,Wang Z,Peng P,Lian J

    更新日期:2019-03-01 00:00:00

  • Anticytokeratins are a potential source of false-positive indirect immunofluorescence assays for C-ANCA.

    abstract::Antibodies to neutrophil cytoplasmic antigens (ANCA) targeted toward granule enzymes have been recognized as a valuable diagnostic tool in the detection of Wegener's granulomatosis and systemic vasculitides. However, the most commonly used method of detection, the indirect immunofluorescence assay, is prone to false-p...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Streicher J,Fabian B,Herkner K,Pointner H,Bayer PM

    更新日期:1998-01-01 00:00:00

  • Analytical Evaluation of Free Testosterone and Cortisol Immunoassays in Saliva as a Reliable Alternative to Serum in Sports Medicine.

    abstract:BACKGROUND:This study was aimed to investigate whether measurement of free testosterone and cortisol in saliva is a reliable alternative to their assessment in serum for monitoring physical fitness in professional athletes. METHODS:We studied 25 members of the soccer team Parma F.C., playing in Italian major football ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Lippi G,Dipalo M,Buonocore R,Gnocchi C,Aloe R,Delsignore R

    更新日期:2016-09-01 00:00:00

  • Accelerated stability model for predicting shelf-life.

    abstract::Second- and higher-order degradation reactions sometimes cannot be approximated with linear or exponential relationships and need to be appropriately modeled. Events above the COULTER HmX Analyzer white blood cell (WBC) counting threshold were recorded for the HmX PAK reagent system stored at five elevated temperature...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Magari RT,Murphy KP,Fernandez T

    更新日期:2002-01-01 00:00:00

  • Biological Variation and Reference Change Value Data for Serum Neuron-Specific Enolase in a Turkish Population.

    abstract:BACKGROUND:Neuron-specific enolase (NSE) is a recognized biomarker for the assessment of cerebral injury in neurological disorders. This study aims to report a definitive assessment of the biological variation (BV) components of this biomarker, including within-subject BV (CVI), between-subject BV (CVG), index of indiv...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Matyar S,Goruroglu Ozturk O,Ziyanoglu Karacor E,Yuzbasioglu Ariyurek S,Sahin G,Kibar F,Yaman A,Inal T

    更新日期:2016-11-01 00:00:00

  • Up-regulation of circular RNA hsa_circ_0037909 promotes essential hypertension.

    abstract:AIMS:Essential hypertension (EH) is a high prevalence disease facing a public health challenge. People were little known about the genetics of diagnosing the cause of EH. Circular RNAs that have a continuous cycle of covalent closure, without affected by RNA exonuclease, and are more stable and hard to degrade may invo...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Bao X,He X,Zheng S,Sun J,Luo Y,Tan R,Zhao J,Zhong F,Zhang L

    更新日期:2019-05-01 00:00:00

  • Association of SOX11 Polymorphisms in distal 3'UTR with Susceptibility for Schizophrenia.

    abstract:BACKGROUND:Diverse and circumstantial evidence suggests that schizophrenia is a neurodevelopmental disorder. Genes contributing to neurodevelopment may be potential candidates for schizophrenia. The human SOX11 gene is a member of the developmentally essential SOX (Sry-related HMG box) transcription factor gene family ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Sun CP,Sun D,Luan ZL,Dai X,Bie X,Ming WH,Sun XW,Huo XX,Lu TL,Zhang D

    更新日期:2020-08-01 00:00:00

  • A comparison study between wide-range and high-sensitivity C-reactive protein assays (Roche Cobas c702) for low C-reactive protein concentration in patients with cardiovascular risk.

    abstract:BACKGROUND:Low concentration C-reactive protein (CRP) has favorable prognostic significance in patients with cardiovascular risks. METHODS:We compared the wr-CRP method with the hs-CRP method both on Roche Cobas c702 analyzer for the determination of low CRP concentration (<20 mg/L) including 200 patients treated in C...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Li R,Xue Y,Wang T,Gong L,Peng P,Xiong P,Dai M,Shao T,Hu Y,Ye X

    更新日期:2019-10-01 00:00:00

  • Pre-miR-27a rs895819 polymorphism and risk of diffuse large B-cell lymphoma.

    abstract:BACKGROUND:Recently, several studies have investigated the relationship between Pre-miR-27a rs895819 polymorphism and risk of various cancers. However, the relationship between rs895819 and diffuse large B-cell lymphoma (DLBCL) has not been well known. METHODS:In this study, we conducted a case-control study to explor...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Tang W,Xu H,Ma D,Ma R,Wu J,Yu X,Feng J,Liu Q

    更新日期:2020-03-01 00:00:00

  • Sunitinib induces primary ectopic endometrial cell apoptosis through up-regulation of STAT1 in vitro.

    abstract:BACKGROUND:Endometriosis (EMS) is a prevalent gynecological condition characterized by the growth of endometrial tissue outside the uterine cavity. This study aimed to clarify the targeted therapeutic effect of sunitinib in an endometriosis in vitro experiment. METHODS:Primary culture of ectopic endometrial cells and ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Li J,Abudula M,Fan X,Wang F,Chen Y,Liu L

    更新日期:2020-11-01 00:00:00

  • Follow-up study on urinary type IV collagen in patients with early stage diabetic nephropathy.

    abstract::Type IV collagen is a major component released from the glomerular and tubular basement membranes. To investigate the alteration of renal type IV collagen turnover in early stage diabetic nephropathy, urinary type IV collagen was measured by a highly sensitive one-step sandwich enzyme immunoassay (EIA). Urinary sample...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Iijima T,Suzuki S,Sekizuka K,Hishiki T,Yagame M,Jinde K,Saotome N,Suzuki D,Sakai H,Tomino Y

    更新日期:1998-01-01 00:00:00

  • Evaluation of Toxocara cati Excretory-Secretory Larval Antigens in Serodiagnosis of Human Toxocariasis.

    abstract:BACKGROUND:Toxocariasis is the clinical term that is applied to infection in the human host with Toxocara species larvae. Serological tests are important tools for the diagnosis of toxocariasis. The aim of this study was to evaluate the excretory-secretory (ES) antigens of T. cati larvae using enzyme-linked immunosorbe...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Zibaei M,Sadjjadi SM,Sarkari B,Uga S

    更新日期:2016-05-01 00:00:00

  • Histocompatibility screening by molecular techniques: use of polymerase chain reaction products and heteroduplex formation.

    abstract::Novel molecular approaches have recently become available allowing improved major histocompatibility complex (MHC) matching of potential allogeneic bone marrow donors and recipients. Current cellular and serological assays are hindered by aberrant cell populations and limited reagents which only detect an individuals'...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Rubocki RJ,Wisecarver JL,Hook DD,Cox SM,Beisel KW

    更新日期:1992-01-01 00:00:00

  • Lipidomic profiling of amniotic fluid and its application in fetal lung maturity prediction.

    abstract:BACKGROUND:The pulmonary surfactant especially lipids in amniotic fluid can reflect the development stage of fetal lung maturity (FLM). However, the conventional lecithin/sphingomyelin (L/S) ratio method by thin layer chromatography (TLC) is insufficient and inconvenient for FLM prediction in clinical practice. METHOD...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Cao Z,Liu J,Xie X,Zhan S,Song W,Wu S,Sun Z,Dong Y,Tang G,Liu Y,Li L,Shen M,Zhai Y,Zou J,Liu X

    更新日期:2020-04-01 00:00:00

  • Ischemia-modified albumin in acute aortic dissection.

    abstract:BACKGROUND:Acute aortic dissection (AOD) is associated with high mortality and early diagnosis and treatment are essential. Ischemia-modified albumin (IMA) is a marker of myocardial ischemia whereas cardiac enzymes are released when myocardial necrosis occurs. We investigated, for the first time, whether IMA increases ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Sbarouni E,Georgiadou P,Marathias A,Panagiotakos D,Geroulanos S,Voudris V

    更新日期:2010-01-01 00:00:00

  • HBV serum markers of 49164 patients and their relationships to HBV genotype in Fujian Province of China.

    abstract:BACKGROUND:There is a high prevalence of Hepatitis B virus (HBV) infection in China. The aim of this article is to reveal the HBV prevalence and its outcome in southern China by analyzing the HBV serum markers and their relationship to HBV genotype. METHODS:HBV serum markers, HBV-DNA and Alanine aminotransferase (ALT)...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Lin S,Liu C,Shang H,Chen H,Yang B,Chen J,Chen Y,Chen D,Ou Q

    更新日期:2013-03-01 00:00:00

  • AKIP1 expression in tumor tissue as a new biomarker for disease monitoring and prognosis in non-small cell lung cancer: Results of a retrospective study.

    abstract:BACKGROUND:A-kinase-interacting protein 1 (AKIP1) has been reported as an oncogenetic factor in multiple cancers; however, no study has reported its role in non-small cell lung cancer (NSCLC) yet. This study aimed to evaluate the expression of AKIP1, and its correlation with tumor characteristics as well as prognosis i...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Liu Y,Tian J,Qin D,Liu J,Xie Y

    更新日期:2020-04-01 00:00:00

  • Reference distributions for complement proteins C3 and C4: a practical, simple and clinically relevant approach in a large cohort.

    abstract::The two serum proteins of the complement cascade in the highest concentrations, C3 and C4, respond to various conditions in much the same manner as do other positive acute-phase proteins. A major difference is that they are relatively sluggish in response to cytokine drive, requiring several days rather than hours to ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Ritchie RF,Palomaki GE,Neveux LM,Navolotskaia O,Ledue TB,Craig WY

    更新日期:2004-01-01 00:00:00

  • Rapid estimation of whole blood everolimus concentrations using architect sirolimus immunoassay and mathematical equations: comparison with everolimus values determined by liquid chromatography/mass spectrometry.

    abstract::United States Food and Drug Administration (FDA) in 2010 approved the use of immunosuppressant drug everolimus, which requires therapeutic drug monitoring in whole blood. Taking advantage of structural similarity between sirolimus and everolimus we attempted to rapidly estimate everolimus concentration from apparent s...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章


    authors: Dasgupta A,Moreno V,Balark S,Smith A,Sonilal M,Tejpal N,Van Buren CT

    更新日期:2011-01-01 00:00:00