Comparison of SemiQuantitative Cotinine Values Obtained by the DRI Immunoassay and Values Obtained by a Liquid Chromatography-Tandem Mass Spectrometry-Based Method: The DRI Immunoassay is Suitable for Screening Purposes Only Because Semiquantitative Value

Abstract:

BACKGROUND:DRI cotinine assay is suitable only for screening for cotinine in urine specimens. We studied the reliability of DRI cotinine semiquantitative values by comparing them with the cotinine concentration obtained with a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. METHODS:Semiquantitative cotinine concentrations in 39 urine specimens obtained by the DRI immunoassay were compared with cotinine concentrations obtained by LC-MS/MS. RESULTS:The DRI cotinine assay consistently overestimated cotinine values obtained by the LC/MS/MS method (y = 1.1529 x + 252.24, n = 39, R2 = 0.8899) indicating that semiquantitative values obtained using the DRI assay may be unreliable. However, no false-negative results were observed using the DRI assay. CONCLUSION:DRI cotinine assay is suitable only for screening cotinine in urine specimens.

journal_name

J Clin Lab Anal

authors

Dixon RB,Dasgupta A

doi

10.1002/jcla.21988

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

1106-1109

issue

6

eissn

0887-8013

issn

1098-2825

journal_volume

30

pub_type

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