Abstract:
:The elevation of chylomicrons and chylomicron remnants in plasma would lead to hyperlipidemia and other complications. Apo B-48, which is translated and produced in the adult intestine from the same gene as Apo B-100, is considered to be an essential component of chylomicrons and chylomicron remnants. Using a peptide representing human Apo B-48 C-terminal sequence as immunogen, we established a monoclonal antibody, B48-151, against human Apo B-48. The specific reactivity for Apo B-48 of this monoclonal antibody was confirmed using Western blot analysis of human plasma in fractions isolated as chylomicron and VLDL. Then, we developed a simple sandwich ELISA method for the detection of human Apo B-48 in serum by combining B48-151 as capturing antibody and HRP-conjugated-polyclonal antibodies for Apo B as signaling antibody. The established sandwich ELISA constitutes a simple method to monitorApo B-48 level in chylomicrons and chylomicron remnants in human serum.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Uchida Y,Kurano Y,Ito Sdoi
10.1002/(sici)1098-2825(1998)12:5<289::aid-jcla7>3subject
Has Abstractpub_date
1998-01-01 00:00:00pages
289-92issue
5eissn
0887-8013issn
1098-2825pii
10.1002/(SICI)1098-2825(1998)12:5<289::AID-JCLA7>3journal_volume
12pub_type
杂志文章abstract::The immune complex transfer enzyme immunoassay for antibody IgG to HIV-1 gp41 antigen was developed using two synthetic peptides. An aliquot (10 microl) of serum samples from HIV-1 seropositive subjects was incubated simultaneously with 2,4-dinitrophenyl-bovine serum albumin-synthetic HIV-1 gp41 peptide conjugates and...
journal_title:Journal of clinical laboratory analysis
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