Virulence factors impair epithelial junctions during bacterial infection.

Abstract:

:Epithelial cells are typically connected through different types of cell junctions that are localized from the apical membrane to the basal surface. In this way, epithelium cells form the first barrier against pathogenic microorganisms and prevent their entry into internal organs and the circulatory system. Recent studies demonstrate that bacterial pathogens disrupt epithelial cell junctions through targeting junctional proteins by secreted virulence factors. In this review, we discuss the diverse strategies used by common bacterial pathogens, including Pseudomonas aeruginosa, Helicobacter pylori, and enteropathogenic Escherichia coli, to disrupt epithelial cell junctions during infection. We also discuss the potential of targeting the pathogenic mechanisms in the treatment of pathogen-associated diseases.

journal_name

J Clin Lab Anal

authors

Zheng M,Sun S,Zhou J,Liu M

doi

10.1002/jcla.23627

subject

Has Abstract

pub_date

2020-10-17 00:00:00

pages

e23627

eissn

0887-8013

issn

1098-2825

pub_type

杂志文章,评审
  • Enterovirus meningitis in Greece from 2003-2005: diagnosis, CSF laboratory findings, and clinical manifestations.

    abstract::Enteroviruses (EVs) are the most commonly identified cause of aseptic meningitis. Rapid detection and characterization of EV meningitis is essential in making decisions for patient management and treatment. A total of 52 cases of acute aseptic meningitis that occurred from March 2003 to April 2005 were investigated fo...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20129

    authors: Dumaidi K,Frantzidou F,Papa A,Diza E,Antoniadis A

    更新日期:2006-01-01 00:00:00

  • Exogenous interferences with Jaffe creatinine assays: addition of sodium dodecyl sulfate to reagent eliminates bilirubin and total protein interference with Jaffe methods.

    abstract:BACKGROUND:The study evaluated the impact of interferences on the analytical specificity of three commercial and commonly used creatinine methods (two Jaffe and one enzymatic). METHODS:Manufacturer creatinine methods plus modified methods were tested with the following interferences: spiking serum with bilirubin, albu...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20350

    authors: Srisawasdi P,Chaichanajarernkul U,Teerakanjana N,Vanavanan S,Kroll MH

    更新日期:2010-01-01 00:00:00

  • New method for calcium on the ADVIA analyzer is free from interference of gadolinium-type contrast agents.

    abstract::Recently, Siemens Diagnostics released a new calcium assay (CA_2) based on complex formation of calcium with Arsenazo III dye for use on the three automated, random access ADVIA Chemistry analyzers (1650, 2400, and 1200). We evaluated this method for analytical performance as well as potential interference from gadoli...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20353

    authors: Datta P,Dasgupta A

    更新日期:2009-01-01 00:00:00

  • HBV serum markers of 49164 patients and their relationships to HBV genotype in Fujian Province of China.

    abstract:BACKGROUND:There is a high prevalence of Hepatitis B virus (HBV) infection in China. The aim of this article is to reveal the HBV prevalence and its outcome in southern China by analyzing the HBV serum markers and their relationship to HBV genotype. METHODS:HBV serum markers, HBV-DNA and Alanine aminotransferase (ALT)...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21573

    authors: Lin S,Liu C,Shang H,Chen H,Yang B,Chen J,Chen Y,Chen D,Ou Q

    更新日期:2013-03-01 00:00:00

  • Distribution characteristics of different human papillomavirus genotypes in women in Wuhan, China.

    abstract:BACKGROUND:Human papillomaviruses (HPVs) are strongly associated with the development of cervical carcinoma, and the distribution of HPV genotypes varies regionally. METHODS:To investigate the distribution characteristics of different genotypes of HPV infection in women in Wuhan, China, a total of 13 775 patients were...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22581

    authors: Xiang F,Guan Q,Liu X,Xiao H,Xia Q,Liu X,Sun H,Song X,Zhong Y,Yuan CH,Xiang Y

    更新日期:2018-10-01 00:00:00

  • Detection of Human Epididymis Protein 4 (HE4) in Human Serum Samples Using a Specific Monoclonal Antibody-Based Sandwich Enzyme-Linked Immunosorbent Assay (ELISA).

    abstract:BACKGROUND:The human epididymis protein 4 (HE4) may have high specificity in the detection of malignant diseases, making the development of an immunoassay for HE4 essential. METHODS:In our study, a fusion gene was constructed encoded with the HE4 protein. This protein was then produced in the bacterial cells (Escheric...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21906

    authors: Zhou L,Lv Z,Shao J,Xu Y,Luo X,Zhang Y,Hu Y,Zhang W,Luo S,Fang J,Wang Y,Duan C,Huang R

    更新日期:2016-09-01 00:00:00

  • A quantitative method for the determination of bosutinib in human plasma using high-performance liquid chromatography and ultraviolet detection.

    abstract:BACKGROUND:We propose a simple, sensitive, and fast high-performance liquid chromatography ultraviolet detection (HPLC-UV) method for the quantitative determination of bosutinib in human plasma. METHODS:Plasma samples were processed using an Oasis hydrophilic-lipophilic balance extraction cartridge (1 mL, 30 mg). Bosu...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22201

    authors: Sumimoto T,Nakahara R,Sato Y,Itoh H

    更新日期:2018-01-01 00:00:00

  • Performance characteristics of a transcription-mediated nucleic acid amplification assay for qualitative detection of hepatitis C virus RNA.

    abstract::The detection of hepatitis C virus (HCV) RNA by nucleic acid amplification techniques is the method of choice to differentiate between ongoing and past infection, and can be used to monitor the course of HCV infection. In this study, we evaluated the performance characteristics of a newly developed transcription-media...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1042

    authors: Ross RS,Viazov SO,Hoffmann S,Roggendorf M

    更新日期:2001-01-01 00:00:00

  • Hsa_circ_0001649 restrains gastric carcinoma growth and metastasis by downregulation of miR-20a.

    abstract:BACKGROUND:Gastric carcinoma (GC) is a familiar carcinoma and serious threat to human health. We investigated the efficacy and mechanism of circular RNA hsa_circ_0001649 on the growth, migration, and invasion of GC cells. METHODS:microRNA (miR)-20a and hsa_circ_0001649 expression was investigated by RT-qPCR and was ch...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23235

    authors: Sun H,Wang Q,Yuan G,Quan J,Dong D,Lun Y,Sun B

    更新日期:2020-06-01 00:00:00

  • In situ hybridization for Helicobacter pylori in gastric mucosal biopsy specimens: quantitative evaluation of test performance in comparison with the CLOtest and thiazine stain.

    abstract::Numerous detection methods for Helicobacter Pylori (H. pylori) have been developed with varying degrees of purported diagnostic utility. We have developed a rapid nonradioactive in situ hybridization (ISH) method for H. pylori detection in paraffin-embedded tissue and assessed its relative diagnostic performance by re...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:

    authors: Barrett DM,Faigel DO,Metz DC,Montone K,Furth EE

    更新日期:1997-01-01 00:00:00

  • A rapid enzyme-linked immunosorbent assay with two modes of detection for measuring cytokine concentration.

    abstract::Interleukin (IL)-6 and IL-8 were measured in 101 serum samples collected from eight intensive-care unit patients using a polystyrene-based stick enzyme-linked immunosorbent assay (STICKELISA) system. This system consisted of an immobilized-antibody ELISA stick and a noncontact spectrophotometer. Cytokine concentration...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20287

    authors: Miwa K,Shibayama N,Moriguchi T,Goto J,Yanagisawa M,Yamazaki Y,Jung G,Matsuda K

    更新日期:2009-01-01 00:00:00

  • After successful hepatitis C virus antiviral therapy: It looks that normal alanine aminotransferase level is not the normal.

    abstract:BACKGROUND:Normal serum alanine aminotransferase (ALT) levels differ with age, gender, and body mass index. Adjusting the upper limits of normal (ULN) for ALT needs further research in different populations. Aim of this work was to monitor the effect of successful chronic hepatitis C (CHC) treatment on the ALT levels i...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22296

    authors: El Kassas M,Alboraie M,Mostafa A,Ezzat R,El Tahan A,Afify S,Sweedy A,Kabbash I,Esmat G

    更新日期:2018-03-01 00:00:00

  • Cost-Effective In-House Neutralization Assay for the Confirmation of HBeAg.

    abstract:BACKGROUND & AIM:Hepatitis B virus-e-antigen (HBeAg) is an affordable viral marker to assess viral replication kinetics and response to antiviral therapy. In the absence of confirmatory assays, discrepant or false-positive HBeAg results are resolved by screening for other HBV markers. We standardized an in-house HBeAg ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21995

    authors: Fletcher GJ,Anantharam R,Radhakrishnan K,Karunakaran A,Abraham P

    更新日期:2016-11-01 00:00:00

  • Reference distributions for complement proteins C3 and C4: a practical, simple and clinically relevant approach in a large cohort.

    abstract::The two serum proteins of the complement cascade in the highest concentrations, C3 and C4, respond to various conditions in much the same manner as do other positive acute-phase proteins. A major difference is that they are relatively sluggish in response to cytokine drive, requiring several days rather than hours to ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.10100

    authors: Ritchie RF,Palomaki GE,Neveux LM,Navolotskaia O,Ledue TB,Craig WY

    更新日期:2004-01-01 00:00:00

  • Genetic analysis of chromosome 22q11.2 markers in congenital heart disease.

    abstract::Congenital heart disease (CHD) is a common cardiac defect found in infants and children. Despite advances in diagnosis and treatment, our understanding of the causative mechanism and etiology of CHD is limited. To determine the genetic etiology of CHD, we selected 11 consecutive short tandem-repeat polymorphic (STRP) ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.10062

    authors: Shi YR,Hsieh KS,Wu JY,Lee CC,Tsai CH,Yu MT,Chang JS,Tsai FJ

    更新日期:2003-01-01 00:00:00

  • A landscape of circulating long non-coding RNA (lncRNA) expression profile and the predictive value of candidate lncRNAs for disease risk of knee osteoarthritis.

    abstract:BACKGROUND:This study aimed to investigate the plasma long non-coding RNA (lncRNA) expression profile in knee osteoarthritis (KOA) patients and the value of candidate lncRNAs for predicting KOA risk. METHODS:Plasma was obtained for RNA sequencing (RNA-seq) in eight KOA patients and eight healthy controls (Ctrls). Ten ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23423

    authors: Liu X,Li M,Zhang B,Zhang N,Feng Q

    更新日期:2020-09-01 00:00:00

  • Circulating level of lipocalin 2 as a predictor of severity in patients with community-acquired pneumonia.

    abstract:BACKGROUND:The aim of this study was to investigate the differential plasma levels of lipocalin 2 (LCN2) and its complex with MMP-9 (where MMP is matrix metalloproteinase) before and after antibiotic treatment in hospitalized adult patients with community-acquired pneumonia (CAP). METHOD:Plasma LCN2 and LCN2/MMP-9 com...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21588

    authors: Yeh YH,Chang JL,Hsiao PC,Tsao SM,Lin CH,Kao SJ,Chou MC,Yang SF,Chien MH

    更新日期:2013-07-01 00:00:00

  • Novel and sensitive noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for alpha-human atrial natriuretic peptide in plasma.

    abstract::A novel and sensitive noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for alpha-human atrial natriuretic peptide (alpha-hANP) in plasma, which uses only one monoclonal IgG for the ring structure of alpha-hANP, is described. Plasma was filtered through polysaccharide membrane to separate peptides...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860050506

    authors: Hashida S,Yamamoto N,Ishikawa E

    更新日期:1991-01-01 00:00:00

  • Clinical value of jointly detection serum lactate dehydrogenase/pleural fluid adenosine deaminase and pleural fluid carcinoembryonic antigen in the identification of malignant pleural effusion.

    abstract:BACKGROUND:Limited data are available for the diagnostic value, and for the diagnostic sensitivity and specificity of joint detection of serum lactate dehydrogenase (sLDH)/pleural fluid adenosine deaminase (pADA) and pleural fluid carcinoembryonic antigen (pCEA) in malignant pleural effusion (MPE). METHODS:We collecte...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22106

    authors: Zhang F,Hu L,Wang J,Chen J,Chen J,Wang Y

    更新日期:2017-09-01 00:00:00

  • Predictive value of antinative DNA for systemic lupus erythematosus: comparison of radioimmunoassay and enzyme immunoassay.

    abstract::Positive predictive values for the diagnosis of systemic lupus erythematosus (SLE) were determined for two antinative DNA assays: a Farr-type radioimmunoassay (RIA) and a solid-phase enzyme immunoassay (EIA). The study population consisted of all patients whose EIA anti-DNA results were positive (greater than 250 IU) ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860030503

    authors: Clough JD,Chang RK

    更新日期:1989-01-01 00:00:00

  • Ferritin in the coronavirus disease 2019 (COVID-19): A systematic review and meta-analysis.

    abstract:OBJECTIVE:The coronavirus disease 2019 (COVID-19) has rapidly developed into a pandemic. Increased levels of ferritin due to cytokine storm and secondary hemophagocytic lymphohistiocytosis were found in severe COVID-19 patients. Therefore, the aim of this study was to determine the role of ferritin in COVID-19. METHOD...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章,meta分析

    doi:10.1002/jcla.23618

    authors: Cheng L,Li H,Li L,Liu C,Yan S,Chen H,Li Y

    更新日期:2020-10-01 00:00:00

  • Contribution of adiponectin polymorphisms to the risk of coronary artery disease in a North-African Tunisian population.

    abstract:BACKGROUND:Adiponectin, an adipocyte-derived protein, is known to play a key role in the processes leading to atherosclerosis and coronary artery disease (CAD) through its anti-atherogenic, anti-inflammatory, antioxidative, and anti-apoptotic properties. In the current study, we have studied the association of two sing...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22446

    authors: Ghazouani L,Elmufti A,Baaziz I,Chaabane I,Ben Mansour H

    更新日期:2018-09-01 00:00:00

  • Comparison of prostate acid phosphatase with acid phosphatase isoenzymes from the lung and spleen.

    abstract::Acid phosphatase was purified to electrophoretic homogeneity from human normal lung and spleen and was characterized biochemically and immunologically in comparison with prostate acid phosphatase (PAP). The apparent MW of lung acid phosphatase (LAP) and spleen acid phosphatase (SAP) was 110,000 and 100,000, respective...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860040606

    authors: Lin MF,Li SS,Chu TM,Lee CL

    更新日期:1990-01-01 00:00:00

  • Different patterns of serum chromogranin A in patients with prostate cancer with and without undergoing hormonal therapy.

    abstract::Elevated serum chromogranin A (CgA) levels have been detected in patients with prostate cancer who have developed resistance to hormonal therapy. We would like to reexamine these cases by using serial specimens to determine whether such elevated levels are also detectable in prostate cancer patients not undergoing hor...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:

    authors: Wu JT,Wu TL,Chang CP,Tsao KC,Sun CF

    更新日期:1999-01-01 00:00:00

  • Preparation and characterization of anti-anti-idiotypic monoclonal antibody ("Ab1-like Ab3") in relation to carcinoembryonic antigen.

    abstract::In order to analyze the epitope structure of carcinoembryonic antigen (CEA) and the idiotype network system, seven anti-idiotypic monoclonal antibodies (anti-Id MoAbs) were generated from a BALB/c mouse immunized with anti-CEA MoAb P1-356, which recognized a synthetic peptide P1 of CEA, domain I. These MoAbs were divi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.10058

    authors: Tsujisaki M,Masuya J,Okada Y,Jinnohara T,Sasaki S,Yachi A,Imai K

    更新日期:2002-01-01 00:00:00

  • Multiple cutaneous infections caused by Prototheca wickerhamii.

    abstract:BACKGROUND:Prototheca species are a group of organisms ubiquitously existing in nature but have become a pathogenic threat to public health, which has aroused wide attention. Species identification and antifungal susceptibility have essential and valuable meanings to clinical diagnosis and treatment. METHODS:A case of...

    journal_title:Journal of clinical laboratory analysis

    pub_type:

    doi:10.1002/jcla.23492

    authors: Zhao F,Chen M,Fu Y

    更新日期:2020-11-01 00:00:00

  • Enzymatic assay for conjugated bilirubin (Bc) in serum using bilirubin oxidase (BOD).

    abstract::We described an automated enzymatic assay for conjugated bilirubin (Bc) in serum using the Iatro D-Bil kit, with bilirubin oxidase (EC 1.3.3.5 BOD) from Myrothecium species. The specificity of the enzyme in the Iatro D-Bil kit was examined by analyzing unconjugated bilirubin (Bu), delta bilirubin (Bdelta), and Bc with...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2825(1999)13:5<219::aid-jcla5>3

    authors: Kimura S,Iyama S,Yamaguchi Y,Hayashi S,Yanagihara T

    更新日期:1999-01-01 00:00:00

  • Significance of aneuploidy in non-Hodgkin lymphomas.

    abstract::The incidence of aneuploidy was determined by flow cytometric analysis in paraffin-embedded tumor samples of 125 patients with non-Hodgkin lymphoma (NHL). There were 48 low-grade (LG) and 77 high-grade (HG) tumors. Aneuploidy was found in 34 (27%) NHL, 15 (31%) LG, and 19 (25%) HG tumors. The analysis of patient survi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860090602

    authors: Vucković J,Forenpoher G,Marusić M,Uzarević B,Sabioncello A,Stula N,Dubravcić M

    更新日期:1995-01-01 00:00:00

  • Rhabdomyosarcoma cell line can be used for the isolation of soluble acetylcholine receptor and for assaying blocking and modulating autoantibodies.

    abstract::We found that the Rhabdomyosarcoma (RD) cell line expresses human acetylcholine receptor (AChR) based on the following evidences: 1. Soluble AChR can be isolated from RD cells following the isolation procedure for AChR from human muscle; 2. Intact RD cells bind to alpha-bungarotoxin (alpha Butx) in a time-dependent an...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860070104

    authors: Wu JT,Astill M,Lloyd C,Salmon VC

    更新日期:1993-01-01 00:00:00

  • Novel and ultrasensitive noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay) for alpha-human atrial natriuretic peptide.

    abstract::A novel and ultrasensitive noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay) for alpha-human atrial natriuretic peptide (alpha-hANP) in plasma is described. alpha-hANP was biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-alpha-hANP [6-28] IgG-coated polystyre...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860060407

    authors: Hashida S,Ishikawa E

    更新日期:1992-01-01 00:00:00