Abstract:
BACKGROUND:Gastric carcinoma (GC) is a familiar carcinoma and serious threat to human health. We investigated the efficacy and mechanism of circular RNA hsa_circ_0001649 on the growth, migration, and invasion of GC cells. METHODS:microRNA (miR)-20a and hsa_circ_0001649 expression was investigated by RT-qPCR and was changed by cell transfection. CCK-8, flow cytometry, and BrdU assays were, respectively, used to investigate the efficacies of hsa_circ_0001649 and miR-20a on cell viability, apoptosis, and proliferation. Transwell assay was used to investigate the efficacies of hsa_circ_0001649 and miR-20a on cell migration and invasion. Moreover, the levels of cyclin D1, Bax, cleaved caspase-3, and signal pathway-related proteins were investigated by Western blot. RESULTS:Hsa_circ_0001649 was downregulated in GC cells and tissues. Upregulation of hsa_circ_0001649 restrained viability, proliferation, migration, and invasion, while promoted apoptosis. Furthermore, miR-20a was negatively regulated by hsa_circ_0001649 and miR-20a overexpression reversed the efficacy of hsa_circ_0001649 upregulation. Finally, upregulation of hsa_circ_0001649 restrained ERK and Wnt/β-catenin pathways while miR-20a overexpression reversed these progresses. CONCLUSION:Upregulation of hsa_circ_0001649 restrained GC cell growth and metastasis by downregulating miR-20a and thereby inactivated ERK and Wnt/β-catenin pathways.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
Sun H,Wang Q,Yuan G,Quan J,Dong D,Lun Y,Sun Bdoi
10.1002/jcla.23235subject
Has Abstractpub_date
2020-06-01 00:00:00pages
e23235issue
6eissn
0887-8013issn
1098-2825journal_volume
34pub_type
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