Evaluation of direct agglutination test (DAT) and ELISA for serodiagnosis of visceral leishmaniasis in India.

abstract：:There is an increasing demand for genetic testing of patients with hypercoagulability. We have developed a multiplex-polymerase chain reaction (PCR) technique that incorporates the state-of-the art of genetic testing for thrombosis mutations (Factor V Leiden [FVL] and G20210A F2 and C46T F12). The sequences are detect...

journal_title：Journal of clinical laboratory analysis

authors： López M,Giraldo P,Alvarez P,Cornudella R,Pocoví M,Martínez A,Fontcuberta J,Soria JM

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n-propanol as internal standard. GC was performed in isothermal mode with a GC run-time of 5.0 min. ...

journal_title：Journal of clinical laboratory analysis

authors： Xiao HT,He L,Tong RS,Yu JY,Chen L,Zou J,Li JQ,Bian Y,Zhang Y

更新日期：2014-09-01 00:00:00

abstract：:Vitamin D plays a vital role not only in bone health but also in pathophysiology of many other body functions. In recent years, there has been significant increase in testing of 25-hydroxyvitamin D (25-OH vitamin D), a marker of vitamin D deficiency. The most commonly used methods for the measurement of 25-OH vitamin ...

journal_title：Journal of clinical laboratory analysis

authors： Garg U,Munar A,Frazee C 3rd,Scott D

更新日期：2012-09-01 00:00:00

abstract：BACKGROUND:Today, the genetic and genomic research entered in a new era of high-throughput genotyping technology. However, mutagenic polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is still a choice of genotyping method in molecular epidemiological research. It has been extensively used fo...

journal_title：Journal of clinical laboratory analysis

authors： Sharma N,Awasthi S,Phadke SR

更新日期：2016-03-01 00:00:00

abstract：:Several retrospective studies have shown that a high level of microsatellite instability (MSI-H) is an important prognostic factor of a more favorable outcome in stage II and III colorectal cancer (CRC) patients. In this study, three commonly used polymerase chain reaction (PCR)-based MSI analysis techniques were comp...

journal_title：Journal of clinical laboratory analysis

authors： Deschoolmeester V,Baay M,Wuyts W,Van Marck E,Pelckmans P,Lardon F,Vermorken JB

更新日期：2006-01-01 00:00:00

abstract：:Ninety semen samples from male patients were studied. They were classified into 4 different groups; normospermic fertile, oligospermia, asthenospermia, and oligoasthenospermia. In addition to routine semen analysis, the hypo-osmotic swelling test was done for all these samples, measuring total protein and specific gra...

journal_title：Journal of clinical laboratory analysis

authors： al-Mogazy HM,al-Khadra S,Kenawy G,al-Zeftawy A,Mostafa T,Attia A,Attia AM

更新日期：1993-01-01 00:00:00

abstract：:To investigate the influence of race, cigarette smoking, and immunologic parameters on serum immunoglobulins, we analyzed serum IgG, IgA, and IgM levels in 455 healthy adults. The study population ranged in age from 20 to 69 years, including 282 whites and 173 blacks, 181 never-smokers, 93 ex-smokers, and 181 current ...

journal_title：Journal of clinical laboratory analysis

authors： Tollerud DJ,Brown LM,Blattner WA,Weiss ST,Maloney EM,Kurman CC,Nelson DL,Hoover RN

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND:This study was aimed to investigate whether measurement of free testosterone and cortisol in saliva is a reliable alternative to their assessment in serum for monitoring physical fitness in professional athletes. METHODS:We studied 25 members of the soccer team Parma F.C., playing in Italian major football ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Dipalo M,Buonocore R,Gnocchi C,Aloe R,Delsignore R

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:Newborn screening tests have been designed to identify infants with severe disorders that are relatively prevalent and treatable or controllable. Comparing to other countries, the incidence of these diseases are very high in Turkey where the rate of consanguineous marriage is high. METHODS:In this article, ...

journal_title：Journal of clinical laboratory analysis

authors： Tezel B,Dilli D,Bolat H,Sahman H,Ozbaş S,Acıcan D,Ertek M,Köse MR,Dilmen U

更新日期：2014-01-01 00:00:00

abstract：BACKGROUND:Blood gas analysis and blood lactate measurement have important roles in patient management. Point-of-care (POC) testing simplifies and provides rapid blood gas and lactate measurements. This study aimed to compare pH, pCO2 , pO2 , and lactate measurements between a POC device and a benchtop blood gas analyz...

journal_title：Journal of clinical laboratory analysis

authors： Indrasari ND,Wonohutomo JP,Sukartini N

更新日期：2019-06-01 00:00:00

abstract：BACKGROUND:Previous studies have demonstrated that dysfunctional regulatory T cells (Tregs) may be associated with Graves' disease (GD). In this study, we evaluated four serum Treg-associated miRNAs (miR-210, miR-182, miR-155, and miR-146a) expressions and assessed the potential of serum miRNAs as biomarkers of GD. ME...

journal_title：Journal of clinical laboratory analysis

authors： Zheng L,Zhuang C,Wang X,Ming L

更新日期：2018-02-01 00:00:00

abstract：BACKGROUND:MicroRNAs play a vital role in coronary artery disease. Abnormal expression of microRNAs has been found to be associated with the occurrence of CAD. METHODS:We identified significantly differentially expressed microRNAs in plasma between 40 patients with CAD and 10 controls with NCA using RNA sequencing. Th...

journal_title：Journal of clinical laboratory analysis

authors： Zhong Z,Zhong W,Zhang Q,Zhang Q,Yu Z,Wu H

更新日期：2020-01-01 00:00:00

abstract：:Numerous detection methods for Helicobacter Pylori (H. pylori) have been developed with varying degrees of purported diagnostic utility. We have developed a rapid nonradioactive in situ hybridization (ISH) method for H. pylori detection in paraffin-embedded tissue and assessed its relative diagnostic performance by re...

journal_title：Journal of clinical laboratory analysis

authors： Barrett DM,Faigel DO,Metz DC,Montone K,Furth EE

更新日期：1997-01-01 00:00:00

abstract：:This study compared the use of conventional urine microscopy, the KOVA system, Neubauer hemacytometry, and the Yellow IRIS for the determination of white blood cell (WBC) and red blood cell (RBC) counts in urine samples. Both KOVA WBC and RBC counts correlated better with the IRIS counts than with conventional microsc...

journal_title：Journal of clinical laboratory analysis

authors： McGinley M,Wong LL,McBride JH,Rodgerson DO

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:The aim of our study was to assess two tumor markers, carbohydrate antigen 724 (CA724) and neuron-specific enolase (NSE), in 20 healthy individuals. Then, we determined their average intra- and inter-individual biological variations (CVI and CVG ). METHODS:Blood samples were taken at weekly intervals for fi...

journal_title：Journal of clinical laboratory analysis

authors： Wang S,Zhao M,Mu R,Zhang X,Yun K,Shang H

更新日期：2018-05-01 00:00:00

abstract：BACKGROUND:Prototheca species are a group of organisms ubiquitously existing in nature but have become a pathogenic threat to public health, which has aroused wide attention. Species identification and antifungal susceptibility have essential and valuable meanings to clinical diagnosis and treatment. METHODS:A case of...

journal_title：Journal of clinical laboratory analysis

authors： Zhao F,Chen M,Fu Y

更新日期：2020-11-01 00:00:00

abstract：:The aim of this study was to evaluate anti-HEV antibody profiles in urine specimens in comparison to corresponding serum samples to assess the utility of urine as a clinical specimen. Paired serum and urine specimens from 71 hepatitis E patients, 33 non-E hepatitis patients, 63 patients with nonhepatic diseases, and 2...

journal_title：Journal of clinical laboratory analysis

authors： Joshi MS,Walimbe AM,Arankalle VA,Chadha MS,Chitambar SD

更新日期：2002-01-01 00:00:00

abstract：:It is believed that boundary compositions of matrix proteins might play a role in stone formation; however, few proteomic studies concerning matrix proteins in urinary stones have been conducted. In this study, we extracted low molecular weight proteins from calcium oxalate stones and measured their characteristic pat...

journal_title：Journal of clinical laboratory analysis

authors： Chen WC,Lai CC,Tsai Y,Lin WY,Tsai FJ

更新日期：2008-01-01 00:00:00

abstract：:The immune complex transfer enzyme immunoassay for antibody IgG to HIV-1 gp41 antigen was developed using two synthetic peptides. An aliquot (10 microl) of serum samples from HIV-1 seropositive subjects was incubated simultaneously with 2,4-dinitrophenyl-bovine serum albumin-synthetic HIV-1 gp41 peptide conjugates and...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Ishikawa S,Hashinaka K,Nishikata I,Oka S,Ishikawa E

更新日期：1998-01-01 00:00:00

abstract：BACKGROUND:Endometriosis (EMS) is a prevalent gynecological condition characterized by the growth of endometrial tissue outside the uterine cavity. This study aimed to clarify the targeted therapeutic effect of sunitinib in an endometriosis in vitro experiment. METHODS:Primary culture of ectopic endometrial cells and ...

journal_title：Journal of clinical laboratory analysis

authors： Li J,Abudula M,Fan X,Wang F,Chen Y,Liu L

更新日期：2020-11-01 00:00:00

abstract：BACKGROUND:Reference intervals are critical for interpreting test results of a clinical laboratory. The aim of this study was to establish local reference intervals of complete blood count for healthy preschoolers in China. METHODS:Three thousand eight hundred and twenty-one blood specimens from children aged 4 months...

journal_title：Journal of clinical laboratory analysis

authors： Wang GC,Li N,Niu C,Ma WB,Wang ZL,Guo H,Han LH

更新日期：2017-09-01 00:00:00

abstract：:Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), ...

journal_title：Journal of clinical laboratory analysis

authors： Itano EN,Ono MA,Sumigawa M,Longo IM,Moura NC,Mendes NF

更新日期：1991-01-01 00:00:00

abstract：BACKGROUND:Early recognition and treatment for severe drug eruption are important in improving clinical outcomes. A few studies have reported laboratory parameters to evaluate the severity of drug eruptions. This study aimed to determine the association between serum ferritin and the severity of drug eruptions. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Pan Y,Tang S,Zheng S,Li S,Zhu D,Fang H,Qiao J

更新日期：2020-02-01 00:00:00

abstract：:A modification of the existing spectrophotometric kinetic method for the determination of pancuronium bromide (PCBr), based on pooled human serum cholinesterase (ChE, EC 3.1.1.8 acylcholine acylhydrolase) inhibition, was developed. Butyrylthiocholine iodide (concentration 1.667 mmol/L) was used as substrate and determ...

journal_title：Journal of clinical laboratory analysis

authors： Stankov-Jovanovic VP,Nikolic-Mandic SD,Mandic LjM,Mitic VD

更新日期：2007-01-01 00:00:00

abstract：BACKGROUNDS:β-Thalassemia is one of the most prevalent worldwide autosomal recessive disorders. It presents a great molecular heterogeneity resulting from more than 200 causative mutations in the β-globin gene. In Tunisia, β-thalassemia represents the most prevalent monogenic hemoglobin disorder with 2.21% of carriers....

journal_title：Journal of clinical laboratory analysis

authors： Sahli CA,Ben Salem I,Jouini L,Laouini N,Dabboubi R,Hadj Fredj S,Siala H,Othmeni R,Dakhlaoui B,Fattoum S,Bibi A,Messaoud T

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the plasma long non-coding RNA (lncRNA) expression profile in knee osteoarthritis (KOA) patients and the value of candidate lncRNAs for predicting KOA risk. METHODS:Plasma was obtained for RNA sequencing (RNA-seq) in eight KOA patients and eight healthy controls (Ctrls). Ten ...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Li M,Zhang B,Zhang N,Feng Q

更新日期：2020-09-01 00:00:00

abstract：:United States Food and Drug Administration (FDA) in 2010 approved the use of immunosuppressant drug everolimus, which requires therapeutic drug monitoring in whole blood. Taking advantage of structural similarity between sirolimus and everolimus we attempted to rapidly estimate everolimus concentration from apparent s...

journal_title：Journal of clinical laboratory analysis

authors： Dasgupta A,Moreno V,Balark S,Smith A,Sonilal M,Tejpal N,Van Buren CT

更新日期：2011-01-01 00:00:00

abstract：:Novel molecular approaches have recently become available allowing improved major histocompatibility complex (MHC) matching of potential allogeneic bone marrow donors and recipients. Current cellular and serological assays are hindered by aberrant cell populations and limited reagents which only detect an individuals'...

journal_title：Journal of clinical laboratory analysis

authors： Rubocki RJ,Wisecarver JL,Hook DD,Cox SM,Beisel KW

更新日期：1992-01-01 00:00:00

abstract：:The levels of several soluble cytokine receptors in body fluids of healthy individuals change with age. Clinical application of the measurement of the serum soluble interleukin-1 receptor type I (sIL-1RI) level depends critically on the samples used as the controls. At present, there is no information regarding the le...

journal_title：Journal of clinical laboratory analysis

authors： Okamoto Y,Tanaka M,Miyahara N,Tanabe M,Gotoh Y,Nagai T,Fujita N,Fukui T,Masuzawa T

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the status of internal quality control (IQC) for cardiac biomarkers from 2011 to 2016 so that we can have overall knowledge of the precision level of measurements in China and set appropriate precision specifications. METHODS:Internal quality control data of cardiac biomarker...

journal_title：Journal of clinical laboratory analysis

authors： Li T,Wang W,Zhao H,He F,Zhong K,Yuan S,Wang Z

更新日期：2018-05-01 00:00:00