Serum Adenosine Deaminase (ADA) Activity: A Novel Screening Test to Differentiate HIV Monoinfection From HIV-HBV and HIV-HCV Coinfections.

Abstract:

BACKGROUND:CD4(+) cell count, the common HIV infection screening test, is costly and unable to differentiate HIV monoinfection from its concurrent infection with hepatitis B or C virus. We aimed to ascertain diagnostic value of serum adenosine deaminase (ADA) activity as a useful tool to differentiate HIV mono- and co-infection. METHODS:Blood samples were collected from 30 HIV-HBV and 30 HIV-HCV coinfected patients, 33 HIV positive subjects, and 72 controls. CD4(+) cell count, serum total ADA (tADA), and ADA1, and ADA2 isoenzyme activities were determined and their sensitivity and specificity were computed. RESULTS:tADA and ADA2 activities were significantly higher and CD4(+) counts were markedly lower in all patients compared with controls. Strong inverse agreements between CD4(+) cell counts and both tADA and ADA2 activities were observed. Serum tADA and ADA1 activities showed the highest specificity and the highest sensitivity, respectively, for differentiating HIV monoinfection from HIV-HBV and HIV-HCV coinfections. CONCLUSIONS:We showed strong agreement and correlation between CD4(+) cell count and ADA enzyme activity. Based on high ADA sensitivity and specificity, it is concluded that determination of ADA activity might be a novel diagnostic tool to distinguish of HIV monoinfection from its coinfection with HBV or HCV.

journal_name

J Clin Lab Anal

authors

Abdi M,Rahbari R,Khatooni Z,Naseri N,Najafi A,Khodadadi I

doi

10.1002/jcla.21836

subject

Has Abstract

pub_date

2016-05-01 00:00:00

pages

200-3

issue

3

eissn

0887-8013

issn

1098-2825

journal_volume

30

pub_type

杂志文章
  • Serological chemiluminescence immunoassay for the diagnosis of SARS-CoV-2 infection.

    abstract:OBJECTIVE:Dynamic monitoring of the concentration variation of IgM and IgG in patients with SARS-CoV-2 infections and exploring their diagnostic value for coronavirus disease-19 (COVID-19). METHODS:A total of 15 patients with SARS-CoV-2 infection were enrolled as the COVID-19 group, and 50 patients were enrolled as th...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23466

    authors: Lijia S,Lihong S,Huabin W,Xiaoping X,Xiaodong L,Yixuan Z,Pin H,Yina X,Xiaoyun S,Junqi W

    更新日期:2020-10-01 00:00:00

  • Establishment of monoclonal antibody against human Apo B-48 and measurement of Apo B-48 in serum by ELISA method.

    abstract::The elevation of chylomicrons and chylomicron remnants in plasma would lead to hyperlipidemia and other complications. Apo B-48, which is translated and produced in the adult intestine from the same gene as Apo B-100, is considered to be an essential component of chylomicrons and chylomicron remnants. Using a peptide ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2825(1998)12:5<289::aid-jcla7>3

    authors: Uchida Y,Kurano Y,Ito S

    更新日期:1998-01-01 00:00:00

  • Decreased serum exosomal miR-29a expression and its clinical significance in papillary thyroid carcinoma.

    abstract:BACKGROUND:Aberrant levels of circulating microRNAs (miRNAs) are potential biomarkers in papillary thyroid carcinoma (PTC) diagnosis and therapy. The aim of this study was to evaluate serum exosomal miR-29a expression as a non-invasive biomarker for PTC diagnosis and prognosis. METHODS:Quantitative reverse transcripti...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23560

    authors: Wen Q,Wang Y,Li X,Jin X,Wang G

    更新日期:2020-12-23 00:00:00

  • The association of NLRP3 and TNFRSF1A polymorphisms with risk of ankylosing spondylitis and treatment efficacy of etanercept.

    abstract:BACKGROUND:To discover how NLRP3 and TNFRSF1A polymorphisms affect the efficacy of traditional medicine and etanercept for ankylosing spondylitis (AS) patients. METHODS:Single nucleotide polymorphism (SNP) and haplotype analyses were conducted based on determined NLRP3 and TNFRSF1A among AS patients. We subsequently a...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22138

    authors: Zhao S,Chen H,Wu G,Zhao C

    更新日期:2017-11-01 00:00:00

  • Factors interfering with the accuracy of five blood glucose meters used in Chinese hospitals.

    abstract:BACKGROUND:The prevalence of diabetes is increasing in China. Glucose control is very important in diabetic patients. The aim of this study was to compare the accuracy of five glucose meters used in Chinese hospitals with a reference method, in the absence and presence of various factors that may interfere with the met...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21611

    authors: Lv H,Zhang GJ,Kang XX,Yuan H,Lv YW,Wang WW,Randall R

    更新日期:2013-09-01 00:00:00

  • Reticulocyte count with maturation fractions in pancytopenic evaluation by a fully automated counter.

    abstract::Using a fully automated reticulocyte counter, the roles of the reticulocyte count with maturation in pancytopenia were evaluated. Different groups of pancytopenia including aplastic anemia, infiltrative marrow disorder, hypersplenism, and megaloblastic anemia were recruited. All patients had bone marrow examinations f...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860070612

    authors: Lin CK,Hsu HC,Chau WK,Jiang ML,Chiu CF

    更新日期:1993-01-01 00:00:00

  • Evaluation of the poly(ADP-ribose) polymerase-1 gene variants in Alzheimer's disease.

    abstract::Amyloid peptide is thought to play a critical role in neuronal death in Alzheimer's disease (AD), most likely through oxidative stress. Free radical-related injury leads to DNA breaks, which subsequently activates the repair enzyme poly(ADP-ribose) polymerase-1 (PARP-1). In this study, the relationship between genetic...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20379

    authors: Liu HP,Lin WY,Wu BT,Liu SH,Wang WF,Tsai CH,Lee CC,Tsai FJ

    更新日期:2010-01-01 00:00:00

  • Serum Clara cell protein and atopic phenotype in children up to 2 years of age.

    abstract:BACKGROUND:Low value of serum Clara cell protein (CC16) is associated with bronchial hyperreactivity in children. OBJECTIVE:To evaluate the serum CC16 in relation to atopy and previously manifested LRTD. METHODS:In the population of 163 healthy 5- to 24-month-old children, atopy was determined by Phadiatop-infant (se...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22151

    authors: Ilic N,Mihailovic N

    更新日期:2017-11-01 00:00:00

  • Association between blood microbiome and type 2 diabetes mellitus: A nested case-control study.

    abstract:BACKGROUND:Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce. METHODS:Fifty incident T2DM cases and 100 matched non-T2DM controls were selected fr...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22842

    authors: Qiu J,Zhou H,Jing Y,Dong C

    更新日期:2019-05-01 00:00:00

  • Twelve years of the Brazilian External Quality Assessment Program in Immunohematology: benefits of the program.

    abstract::The Brazilian External Quality Assessment Program in Immunohematology (BEQAPI) was introduced with the objective of evaluating the quality of diagnosis in immunohematology. From 1992 to 2003, proficiency tests for ABO grouping, Rh (D, C, c, E, e), K phenotyping, direct antiglobulin testing (DAT), antibody screening (A...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20080

    authors: Melo L,Pellegrino J Jr,Bianco C,Castilho L

    更新日期:2005-01-01 00:00:00

  • Lectin affinity electrophoresis of serum alkaline phosphatase in metastasized breast cancer.

    abstract::The use of serum alkaline phosphatase (ALP) isoenzymes as markers of breast cancer metastases and treatment efficacy has received little attention. Twenty-six breast cancer women (56+/-13 years, all post-menopausal) were prospectively evaluated during their first and third course of chemotherapy (4-week interval). Ser...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20357

    authors: Le Bricon T,Gay-Bellile C,Cottu P,Benlakehal M,Guillon H,Houzé P

    更新日期:2010-01-01 00:00:00

  • Clinical and laboratory evaluation of idiopathic male infertility in a secondary referral center in India.

    abstract::The genetic basis of infertility has received increasing recognition in recent years, particularly with the advent of assisted reproductive technology. It is now becoming obvious that genetic etiology for infertility is an important cause of disrupted spermatogenesis. Y-chromosome microdeletions and abnormal karyotype...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20216

    authors: Abid S,Maitra A,Meherji P,Patel Z,Kadam S,Shah J,Shah R,Kulkarni V,Baburao V,Gokral J

    更新日期:2008-01-01 00:00:00

  • Up-regulation of circular RNA hsa_circ_0037909 promotes essential hypertension.

    abstract:AIMS:Essential hypertension (EH) is a high prevalence disease facing a public health challenge. People were little known about the genetics of diagnosing the cause of EH. Circular RNAs that have a continuous cycle of covalent closure, without affected by RNA exonuclease, and are more stable and hard to degrade may invo...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22853

    authors: Bao X,He X,Zheng S,Sun J,Luo Y,Tan R,Zhao J,Zhong F,Zhang L

    更新日期:2019-05-01 00:00:00

  • Genotypic effect of a mutation of the MYBPC3 gene and two phenotypes with different patterns of inheritance.

    abstract:BACKGROUND:MYBPC3 mutations have been described in dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM). A mutation, c.3373G>A, has been reported to cause autosomal recessive form of HCM. Here, we report that this mutation can cause autosomal dominant form of DCM. METHODS:Next-generation sequencing using...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22419

    authors: Mahdieh N,Hosseini Moghaddam M,Motavaf M,Rabbani A,Soveizi M,Maleki M,Rabbani B,Alizadeh-Asl A

    更新日期:2018-07-01 00:00:00

  • Useful information provided by graphic displays of automated cell counter in hematological malignancies.

    abstract:BACKGROUND:Automated cell counters have become more and more sophisticated with passing years. The numerical and graphic data both provide useful clues for suspecting a diagnosis especially when the workload is very high. AIM:We present our experience of useful information provided by graphic displays of an automated ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22392

    authors: Gupta M,Chauhan K,Singhvi T,Kumari M,Grover RK

    更新日期:2018-06-01 00:00:00

  • Circulating microRNA expression profiling and bioinformatics analysis of patients with coronary artery disease by RNA sequencing.

    abstract:BACKGROUND:MicroRNAs play a vital role in coronary artery disease. Abnormal expression of microRNAs has been found to be associated with the occurrence of CAD. METHODS:We identified significantly differentially expressed microRNAs in plasma between 40 patients with CAD and 10 controls with NCA using RNA sequencing. Th...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23020

    authors: Zhong Z,Zhong W,Zhang Q,Zhang Q,Yu Z,Wu H

    更新日期:2020-01-01 00:00:00

  • The clinical value of lncRNA MALAT1 and its targets miR-125b, miR-133, miR-146a, and miR-203 for predicting disease progression in chronic obstructive pulmonary disease patients.

    abstract:OBJECTIVE:The study aimed to explore the correlations of long non-coding RNA MALAT1 (lncRNA MALAT1) and its targets microRNA (miR)-125b, miR-133, miR-146a, and miR-203 with acute exacerbation risk, inflammation, and disease severity of chronic obstructive pulmonary disease (COPD). METHODS:Plasma samples were obtained ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23410

    authors: Liu S,Liu M,Dong L

    更新日期:2020-09-01 00:00:00

  • Relative quantification of collagen mRNA in fibroblasts by a radioactive polymerase chain reaction technique.

    abstract::A radioactive polymerase chain reaction (PCR) method has been developed for the relative quantification of the human alpha-2 chain of type I collagen [hu alpha-2(I)] in cells. cDNAs generated by reverse transcription from the total pool of cytoplasmic RNA serve as a template for polymerase chain reaction amplification...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860050407

    authors: Yu YL,Golden JA,Migchielsen AA,Goetzl EJ,Turck CW

    更新日期:1991-01-01 00:00:00

  • Colorimetric Detection by Gold Nanoparticle DNA Probes for Miltenberger Series (GP.Mur, GP.Hop, and GP.Bun) Identification.

    abstract:BACKGROUND:Miltenberger (Mi) series are the collective glycophorin hybrids in the MNS blood group system. Mi series are composed of several subtypes, for examples, GP.Mur, GP.Hop, and GP.Bun. The incompatibility of Mi series blood transfusion poses the risk of hemolysis. Due to the lack of standard antibodies for Mi se...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21951

    authors: Vongsakulyanon A,Pipatpanukul C,Kitpoka P,Kunakorn M,Srikhirin T

    更新日期:2016-11-01 00:00:00

  • A paternal t(6;22)(q25.3;p12) leading to a deleted and satellited der(6) in a short-lived infant.

    abstract:BACKGROUND:Non-acrocentric satellited chromosomes mostly result from familial balanced insertions or translocations with p12 or p13 of any acrocentric. Although all non-acrocentrics have been involved, only 12 instances of chromosome 6 involvement are known. CASE PRESENTATION:A female infant exhibited clinical feature...

    journal_title:Journal of clinical laboratory analysis

    pub_type:

    doi:10.1002/jcla.23355

    authors: Domínguez MG,Rivera H,Dávalos-Pulido RM,Dávalos-Rodríguez IP

    更新日期:2020-08-01 00:00:00

  • Metabolic pathways related to oxidative stress in patients with hemoglobin h disease and iron overload.

    abstract:BACKGROUND:Iron overload is a major complication in patients with hemoglobin H (Hb H) disease and causes damage of tissues. METHODS:We investigated 26 Hb H patients and 75 controls to evaluate their oxidative stress and antioxidant statuses. RESULTS:There were significantly increased levels of superoxide anion in leu...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21676

    authors: Chiou SS,Tsao CJ,Tsai SM,Wu YR,Liao YM,Lin PC,Tsai LY

    更新日期:2014-07-01 00:00:00

  • Serum pepsinogen C: a useful marker of Helicobacter pylori eradication?

    abstract::Medical treatment for Helicobacter pylori (Hp) infection is now recommended in several types of gastroduodenal disease, and its success is usually monitored by hystology. The end-points of our work were to identify the most suitable serum index of Hp eradication among pepsinogen A (PGA), pepsinogen C (PGC), PGA/PGC ra...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2825(1996)10:1<1::AID-JCLA1>3.0

    authors: Plebani M,Basso D,Scrigner M,Toma A,Di Mario F,Dal Bò N,Samloff IM

    更新日期:1996-01-01 00:00:00

  • Decreased frequency of circulating Th9 cells in patients with chronic hepatitis B infection.

    abstract:BACKGROUND:Chronic hepatitis B (CHB) is one of the major infectious diseases in which CD4+ T helper (Th) cells play a crucial role. Th9 cells are a distinct subset of CD4+ Th cells with important physiological functions. OBJECTIVE:The study aimed to assess the potential involvement of Th9 cells in the inadequate immun...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22246

    authors: Cui M,Lv Y,Lu J,Zhang W,Duan Y,Huang Y,Yang L,Li M,Liu W,Liu D,Yan H

    更新日期:2018-02-01 00:00:00

  • Multivariable index for assessing the activity and predicting all-cause mortality in antineutrophil cytoplasmic antibody-associated vasculitis.

    abstract:BACKGROUND:So far, there has been no tool to estimate activity at diagnosis and predict all-cause mortality in patients with ANCA-associated vasculitis (AAV). Hence, we determined the initial predictors of them in patients with AAV. METHODS:We retrospectively reviewed the medical records of 182 patients with AAV. Seve...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23022

    authors: Kim MK,Choi H,Kim JY,Song JJ,Park YB,Lee SW

    更新日期:2020-01-01 00:00:00

  • Isolation and characterizations of clarithromycin-resistant Mycobacterium avium clinical isolates.

    abstract::Mycobacterium avium is an important intracellular pathogen, particularly in AIDS patients. It also shows the second frequency among nontuberculous mycobacteria infections in Korea. Point mutations of domain V region of the 23S rRNA gene has been known to confer clarithromycin resistance to M. avium. In order to isolat...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20403

    authors: Lee SH,Park YK,Kim CK,Kim HJ

    更新日期:2011-01-01 00:00:00

  • Evaluation of the analytical performance of endocrine analytes using sigma metrics.

    abstract:BACKGROUND:(a) To evaluate the clinical performance of endocrine analytes using the sigma metrics (σ) model. (b) To redesign quality control strategies for performance improvement. METHODS:The sigma values of the analytes were initially evaluated based on the allowable total error (TEa), bias, and coefficient of varia...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23581

    authors: Liu Y,Cao Y,Liu X,Wu L,Cai W

    更新日期:2020-09-20 00:00:00

  • Simultaneous determination of free and total prostate-specific antigen by a magnetic particle-based time-resolved fluoroimmunoassay.

    abstract:OBJECTIVES:In this paper, a novel, sensitive and rapid method to simultaneously determine the free and total prostate-specific antigen (fPSA and tPSA) in serum by combining a time-resolved fluoroimmunoassay (TRFIA) and immunomagnetic separation was described. METHODS:The new approach uses magnetic particles as an immo...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22137

    authors: Zhou B,Zhang J,Lv Z,Fan J,Zhang Y,Huang B

    更新日期:2017-11-01 00:00:00

  • Evaluation of conductivity-based osmolality measurement in urine using the Sysmex UF5000.

    abstract:BACKGROUND:Automated flow cytometry-based urine analyzer is increasingly being used to identify and enumerate cells and particles in urine specimens. It measures electrical conductivity which could be transformed to osmolality. Using this machine, all urine specimens could be screened for osmolality without requiring a...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23586

    authors: Yoo DW,Lee SM,Moon SY,Kim IS,Chang CL

    更新日期:2020-09-24 00:00:00

  • Anticyclic citrullinated peptide antibody and rheumatoid factor in south Tunisian patients with rheumatoid arthritis: association with disease activity and severity.

    abstract:OBJECTIVE:To explore relationships between immunological status, clinical features, radiographic damage, disease activity, and functional disability in Tunisian patients with rheumatoid arthritis (RA). MATERIALS AND METHODS:The study was carried out in 112 patients with RA. Demographic characteristics, disease duratio...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21638

    authors: Hamad MB,Marzouk S,Kaddour N,Masmoudi H,Fakhfakh F,Rebai A,Bahloul Z,Maalej A

    更新日期:2014-01-01 00:00:00

  • Automated chemiluminescent assay for C-peptide.

    abstract::C-peptide is secreted in equimolar concentrations with insulin, and is often measured to assess pancreatic beta-cell function. C-peptide analysis is most often performed by radioimmunoassay (RIA) which has several disadvantages. We evaluated an automated, chemiluminescent immunoassay for C-peptide in terms of precisio...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:

    authors: Hardy RW,Cohn M,Konrad RJ

    更新日期:2000-01-01 00:00:00