Genotypic effect of a mutation of the MYBPC3 gene and two phenotypes with different patterns of inheritance.

abstract：BACKGROUND:Blood gas analysis and blood lactate measurement have important roles in patient management. Point-of-care (POC) testing simplifies and provides rapid blood gas and lactate measurements. This study aimed to compare pH, pCO2 , pO2 , and lactate measurements between a POC device and a benchtop blood gas analyz...

journal_title：Journal of clinical laboratory analysis

authors： Indrasari ND,Wonohutomo JP,Sukartini N

更新日期：2019-06-01 00:00:00

abstract：:An optimized reaction condition for amplification of influenza A virus RNA, by thermus thermophilus (Tth) DNA polymerase-based PCR, incorporating uracil N glycosylase (UNG) and dUTP in the reaction has been determined. dUTP could not be substituted for all dTTP sites when UNG was present in the reaction. The relative ...

journal_title：Journal of clinical laboratory analysis

authors： Poddar SK,Sawyer MH,Connor JD

更新日期：1997-01-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum-absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum-absorbed te...

journal_title：Journal of clinical laboratory analysis

authors： Thiele GM,Rogers J,Collins M,Yasuda N,Smith D,McDonald TL

更新日期：1990-01-01 00:00:00

abstract：:The use of serum alkaline phosphatase (ALP) isoenzymes as markers of breast cancer metastases and treatment efficacy has received little attention. Twenty-six breast cancer women (56+/-13 years, all post-menopausal) were prospectively evaluated during their first and third course of chemotherapy (4-week interval). Ser...

journal_title：Journal of clinical laboratory analysis

authors： Le Bricon T,Gay-Bellile C,Cottu P,Benlakehal M,Guillon H,Houzé P

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:Acute aortic dissection (AOD) is associated with high mortality and early diagnosis and treatment are essential. Ischemia-modified albumin (IMA) is a marker of myocardial ischemia whereas cardiac enzymes are released when myocardial necrosis occurs. We investigated, for the first time, whether IMA increases ...

journal_title：Journal of clinical laboratory analysis

authors： Sbarouni E,Georgiadou P,Marathias A,Panagiotakos D,Geroulanos S,Voudris V

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:We evaluated the new body fluid module on Sysmex UF1000-i (UF1000i-BF) for analysis of white blood cell (WBC) and red blood cell (RBC) in cerebrospinal fluid (CSF). METHODS:WBC and RBC counting were compared between UF1000i-BF and Fuchs-Rosenthal counting chamber in 67 CSF samples. This study also included ...

journal_title：Journal of clinical laboratory analysis

authors： Buoro S,Apassiti Esposito S,Alessio M,Crippa A,Ottomano C,Lippi G

更新日期：2016-09-01 00:00:00

abstract：BACKGROUND:Acute respiratory distress syndrome (ARDS) is a critical condition characterized by bilateral pulmonary infiltrates and severe hypoxemia. This study aimed to evaluate the diagnostic and prognostic values of Club cell protein 16 (CC16) in critical care patients with ARDS. METHODS:In this retrospective observ...

journal_title：Journal of clinical laboratory analysis

authors： Lin J,Zhang W,Wang L,Tian F

更新日期：2018-02-01 00:00:00

abstract：BACKGROUND:Enzyme-linked immunosorbent assay (ELISA) has traditionally been used to detect myeloperoxidase (MPO) and proteinase 3 (PR3) antibodies, although it is time-consuming and physically demanding. As a novel and highly effective immunoassay, we compared chemiluminescent immunoassay (CIA) with ELISA to verify the...

journal_title：Journal of clinical laboratory analysis

authors： Hou X,Liu J,Wang T,Zhou J,Cui L

更新日期：2020-10-09 00:00:00

abstract：:The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modifi...

journal_title：Journal of clinical laboratory analysis

authors： Gutiérrez J,Rodríguez MJ,De Ory F,Piédrola G,Maroto MC

更新日期：1999-01-01 00:00:00

abstract：BACKGROUND:The trends in usage of tumor markers, including CEA, SCC, NSE, Cyfra21-1, and ProGRP, in Chinese lung cancer patients in the real-world setting are not fully investigated. METHODS:A retrospective descriptive study was conducted using the database of Qilu Hospital of Shandong University, China between Januar...

journal_title：Journal of clinical laboratory analysis

authors： Wang H,Zhang J,Li X,Zhang C,Zheng S,Chi Y,Sheng X,Zhang Y

更新日期：2020-11-01 00:00:00

abstract：:Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), ...

journal_title：Journal of clinical laboratory analysis

authors： Itano EN,Ono MA,Sumigawa M,Longo IM,Moura NC,Mendes NF

更新日期：1991-01-01 00:00:00

abstract：BACKGROUND:Although a diagnosis of infectious diseases is essential for timely treatment, the performance of diagnostic tests has been hardly evaluated due to variable results that are influenced by multiple factors in different conditions. In the present study, the performance of the Alinity i system, which is a newly...

journal_title：Journal of clinical laboratory analysis

authors： Nam M,Song DY,Song SH,Roh EY,Shin S,Park KU,Song EY

更新日期：2020-12-07 00:00:00

abstract：BACKGROUND:Gastric carcinoma (GC) is a familiar carcinoma and serious threat to human health. We investigated the efficacy and mechanism of circular RNA hsa_circ_0001649 on the growth, migration, and invasion of GC cells. METHODS:microRNA (miR)-20a and hsa_circ_0001649 expression was investigated by RT-qPCR and was ch...

journal_title：Journal of clinical laboratory analysis

authors： Sun H,Wang Q,Yuan G,Quan J,Dong D,Lun Y,Sun B

更新日期：2020-06-01 00:00:00

abstract：BACKGROUND:Contradictory results have been reported previously in the analyses of cross-reactivity among Blomia tropicalis (Blo t), Dermatophagoides pteronyssinus (Der p), and Dermatophagoides farinae (Der f). This study aims to investigate the characteristics of co-sensitization and the IgE cross-reactivity among them...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Zheng P,Zheng SG,Zhai Y,Zhao X,Chen Y,Cai C,Wu Z,Huang Z,Zou X,Liao C,Sun B

更新日期：2019-11-01 00:00:00

abstract：:We have evaluated a new latex nephelometric test for the quantitation of myoglobin in human serum. The assay consists of incubating the diluted serum sample (20-fold) for 12 min at room temperature with latex particles covalently coated with anti-myoglobin antibodies and then quantifying the change of light-scatter pr...

journal_title：Journal of clinical laboratory analysis

authors： Borque L,Rus A,Ruiz R

更新日期：1991-01-01 00:00:00

abstract：:Quantitation of hemoglobin alpha 2 delta 2 (HbA2) is a basic and confirmatory test in diagnosing the carrier state of beta-thalassemia. The present study was designed to investigate the effect of cigarette smoking on the diagnostic reliability of HbA2. A total of 2,867 (654 smokers and 2,213 never smokers) male subjec...

journal_title：Journal of clinical laboratory analysis

authors： Tarazi IS,Sirdah MM,El Jeadi H,Al Haddad RM

更新日期：2008-01-01 00:00:00

abstract：BACKGROUND:Sustaining proliferation is the most fundamental step for breast cancer tumor genesis. Accelerated proliferation is usually linked to the uncontrolled cell cycle. However, the internal and external factors linked to the activation of breast cancer cell cycle are still to be investigated. METHODS:quantitativ...

journal_title：Journal of clinical laboratory analysis

authors： Ye RY,Kuang XY,Zeng HJ,Shao N,Lin Y,Wang SM

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND:Information on serum albumin and trace elements among children suffering from Sickle Cell Anemia (SCA) was poorly documented in Africa. The aim of this study was to describe and to compare different values of trace elements with published reports from other parts of the world. METHODS:We carried out a case-...

journal_title：Journal of clinical laboratory analysis

authors： Sungu JK,Mukuku O,Mutombo AM,Mawaw P,Aloni MN,Luboya ON

更新日期：2018-01-01 00:00:00

abstract：:To investigate the influence of race, cigarette smoking, and immunologic parameters on serum immunoglobulins, we analyzed serum IgG, IgA, and IgM levels in 455 healthy adults. The study population ranged in age from 20 to 69 years, including 282 whites and 173 blacks, 181 never-smokers, 93 ex-smokers, and 181 current ...

journal_title：Journal of clinical laboratory analysis

authors： Tollerud DJ,Brown LM,Blattner WA,Weiss ST,Maloney EM,Kurman CC,Nelson DL,Hoover RN

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND:The pulmonary surfactant especially lipids in amniotic fluid can reflect the development stage of fetal lung maturity (FLM). However, the conventional lecithin/sphingomyelin (L/S) ratio method by thin layer chromatography (TLC) is insufficient and inconvenient for FLM prediction in clinical practice. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Cao Z,Liu J,Xie X,Zhan S,Song W,Wu S,Sun Z,Dong Y,Tang G,Liu Y,Li L,Shen M,Zhai Y,Zou J,Liu X

更新日期：2020-04-01 00:00:00

abstract：:The levels of serum IgA and C3 in patients with IgA nephropathy were determined using international standard serum (IFCC/CRM470) in a multicenter trial in Japan. The ratio of serum IgA to C3 (serum IgA/C3 ratio) without any information from renal biopsy was used for the diagnosis of IgA nephropathy. Three hundred and ...

journal_title：Journal of clinical laboratory analysis

authors： Tomino Y,Suzuki S,Imai H,Saito T,Kawamura T,Yorioka N,Harada T,Yasumoto Y,Kida H,Kobayashi Y,Endoh M,Sato H,Saito K

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:Recently, several studies have investigated the relationship between Pre-miR-27a rs895819 polymorphism and risk of various cancers. However, the relationship between rs895819 and diffuse large B-cell lymphoma (DLBCL) has not been well known. METHODS:In this study, we conducted a case-control study to explor...

journal_title：Journal of clinical laboratory analysis

authors： Tang W,Xu H,Ma D,Ma R,Wu J,Yu X,Feng J,Liu Q

更新日期：2020-03-01 00:00:00

abstract：BACKGROUND:For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n-propanol as internal standard. GC was performed in isothermal mode with a GC run-time of 5.0 min. ...

journal_title：Journal of clinical laboratory analysis

authors： Xiao HT,He L,Tong RS,Yu JY,Chen L,Zou J,Li JQ,Bian Y,Zhang Y

更新日期：2014-09-01 00:00:00

abstract：BACKGROUND:The aim of this study was to explain the effects of microRNA-132 in renal cell carcinoma by regulating FOXM1 expression. METHODS:Thirty patients with renal cell carcinoma admitted to our hospital were enrolled, and their adjacent normal tissues and cancer tissues were taken. The expression of microRNA-132 w...

journal_title：Journal of clinical laboratory analysis

authors： Yu Y,Lu W,Zhou X,Huang H,Shen S,Guo L

更新日期：2020-01-01 00:00:00

abstract：:Adenosine deaminase (ADA) exists as two isoenzymes, ADA(1) and ADA(2). It appears that the ADA(2) isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA(2). The aim of this prospective study was to analyse ADA isoenzymes in the CSF of patie...

journal_title：Journal of clinical laboratory analysis

authors： Schutte CM,Ungerer JP,du Plessis H,van der Meyden CH

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:Prototheca species are a group of organisms ubiquitously existing in nature but have become a pathogenic threat to public health, which has aroused wide attention. Species identification and antifungal susceptibility have essential and valuable meanings to clinical diagnosis and treatment. METHODS:A case of...

journal_title：Journal of clinical laboratory analysis

authors： Zhao F,Chen M,Fu Y

更新日期：2020-11-01 00:00:00

abstract：BACKGROUND:To discover how NLRP3 and TNFRSF1A polymorphisms affect the efficacy of traditional medicine and etanercept for ankylosing spondylitis (AS) patients. METHODS:Single nucleotide polymorphism (SNP) and haplotype analyses were conducted based on determined NLRP3 and TNFRSF1A among AS patients. We subsequently a...

journal_title：Journal of clinical laboratory analysis

authors： Zhao S,Chen H,Wu G,Zhao C

更新日期：2017-11-01 00:00:00

abstract：BACKGROUND:Identification of dermatophytes at the species level, relying on macro- and microscopic properties of the colonies is time-consuming, questioned in many circumstances, and requires considerable expertise. In this study, we examined the potency of a new genetic marker, β-tubulin (BT2) gene, for differentiatio...

journal_title：Journal of clinical laboratory analysis

authors： Abastabar M,Mirhendi H,Rezaei-Matehkolaei A,Shidfar MR,Kordbacheh P,Makimura K

更新日期：2014-03-01 00:00:00

abstract：BACKGROUND:Standards play an important role in detection of the BCR-ABL1 fusion gene (FG) transcript. However, the standards widely used in laboratories are mainly based on plasmids or cDNA, which cannot accurately reflect the process of RNA extraction and cDNA synthesis. Therefore, we aimed to develop armored RNA-base...

journal_title：Journal of clinical laboratory analysis

authors： Fu Y,Zhang R,Wu Q,Zhang J,Bao L,Li J

更新日期：2018-11-01 00:00:00

abstract：BACKGROUND:Though recent reports have indicated a higher prevalence of latent tuberculosis infection (LTBI) in laboratory personnel than in other healthcare workers, these studies included only a limited number of laboratory personnel. METHODS:We have thus focused on the laboratory personnel, who had a high level of e...

journal_title：Journal of clinical laboratory analysis

authors： Moon HW,Kim H,Hur M,Yun YM,Lee A

更新日期：2011-11-01 00:00:00