Optimized PCR amplification of influenza A virus RNA using Tth DNA polymerase, incorporating uracil N glycosylase (UNG) in a single tube reaction.

abstract：BACKGROUNDS:β-Thalassemia is one of the most prevalent worldwide autosomal recessive disorders. It presents a great molecular heterogeneity resulting from more than 200 causative mutations in the β-globin gene. In Tunisia, β-thalassemia represents the most prevalent monogenic hemoglobin disorder with 2.21% of carriers....

journal_title：Journal of clinical laboratory analysis

authors： Sahli CA,Ben Salem I,Jouini L,Laouini N,Dabboubi R,Hadj Fredj S,Siala H,Othmeni R,Dakhlaoui B,Fattoum S,Bibi A,Messaoud T

更新日期：2016-09-01 00:00:00

abstract：:Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections. The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis identification. fbl gene, encoding a fibrinogen-binding adhesin, was exploited and assessed ...

journal_title：Journal of clinical laboratory analysis

authors： Chatzigeorgiou KS,Siafakas N,Petinaki E,Zerva L

更新日期：2010-01-01 00:00:00

abstract：:It is well established that the targeted receptor for ciguatoxin (CTX) in mammalian tissues is the sodium channel, affecting the influx of sodium into cells and altering the action potential and function of the cell. Since the syntheses of fragments of CTX has become available, our focus has been on the receptor funct...

journal_title：Journal of clinical laboratory analysis

authors： Hokama Y,Chun KE,Campora CE,Higa N,Suma C,Hamajima A,Isobe M

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:This study was performed to compare the clinical efficacies of anterior cruciate ligament (ACL) reconstruction with autologous ligament grafting at different time points. METHODS:Eighty-five patients with ACL were categorized into two groups: Group A (GA, n = 45), who underwent early-stage (≤3 weeks) surger...

journal_title：Journal of clinical laboratory analysis

authors： Hu B,Gao F,Li C,Zhang B,An M,Lu M,Liu Y,Liu Y

更新日期：2020-08-26 00:00:00

abstract：:A microparticle-enhanced nephelometric immunoassay was developed for lactoferrin quantitation in human milk. It is based on the nephelometric measurement of the light scattered during the competitive immuno-agglutination of a microparticle-lactoferrin conjugate with an antilactoferrin antiserum. This immunoassay is se...

journal_title：Journal of clinical laboratory analysis

authors： Cuillière ML,Montagne P,Molé C,Béné MC,Faure G

更新日期：1997-01-01 00:00:00

abstract：:Adenosine deaminase (ADA) exists as two isoenzymes, ADA(1) and ADA(2). It appears that the ADA(2) isoenzyme originates mainly from monocytes and macrophages. In tuberculous pleural effusions most of the ADA activity consists of ADA(2). The aim of this prospective study was to analyse ADA isoenzymes in the CSF of patie...

journal_title：Journal of clinical laboratory analysis

authors： Schutte CM,Ungerer JP,du Plessis H,van der Meyden CH

更新日期：2001-01-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the plasma long non-coding RNA (lncRNA) expression profile in knee osteoarthritis (KOA) patients and the value of candidate lncRNAs for predicting KOA risk. METHODS:Plasma was obtained for RNA sequencing (RNA-seq) in eight KOA patients and eight healthy controls (Ctrls). Ten ...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Li M,Zhang B,Zhang N,Feng Q

更新日期：2020-09-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum-absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum-absorbed te...

journal_title：Journal of clinical laboratory analysis

authors： Thiele GM,Rogers J,Collins M,Yasuda N,Smith D,McDonald TL

更新日期：1990-01-01 00:00:00

abstract：AIM:To evaluate, retrospectively, the frequency of autoantibodies of antiphospholipid syndrome (APLS) in Tunisian patients with primary biliary cirrhosis (PBC). PATIENTS AND METHODS:We analyzed 80 PBC sera and 80 sera from blood donors. ELISA was used to determine the frequency of antibodies against cardiolipin (aCL I...

journal_title：Journal of clinical laboratory analysis

authors： Mankaï A,Manoubi W,Ghozzi M,Melayah S,Sakly W,Ghedira I

更新日期：2015-01-01 00:00:00

abstract：:The genetic basis of infertility has received increasing recognition in recent years, particularly with the advent of assisted reproductive technology. It is now becoming obvious that genetic etiology for infertility is an important cause of disrupted spermatogenesis. Y-chromosome microdeletions and abnormal karyotype...

journal_title：Journal of clinical laboratory analysis

authors： Abid S,Maitra A,Meherji P,Patel Z,Kadam S,Shah J,Shah R,Kulkarni V,Baburao V,Gokral J

更新日期：2008-01-01 00:00:00

abstract：:We here describe an ion-exchange high-performance liquid chromatography technique with electrochemical detection for rapid quantification of glutathione, homocysteine, cysteinylglycine, and methionine. The analytical validation of the technique showed within-assay and between-assay coefficients of variation between 3....

journal_title：Journal of clinical laboratory analysis

authors： Houze P,Gamra S,Madelaine I,Bousquet B,Gourmel B

更新日期：2001-01-01 00:00:00

abstract：:The effects of TPA (12-0-tetradecanoylphorbol-13-acetate) and RA (retinoic acid) were investigated on the cell lines HL60 (acute promyelocytic leukemia) and K562 (erythroleukemia) and on cells from patients with several kinds of leukemia. There were 14 cases of acute lymphocytic leukemia (ALL), 2 cases of chronic lymp...

journal_title：Journal of clinical laboratory analysis

authors： Marques-Silva VM,De Souza MH,Teixeira MC,Arcuri RA,Rumjanek VM

更新日期：1990-01-01 00:00:00

abstract：BACKGROUND:Recently, several studies have investigated the relationship between Pre-miR-27a rs895819 polymorphism and risk of various cancers. However, the relationship between rs895819 and diffuse large B-cell lymphoma (DLBCL) has not been well known. METHODS:In this study, we conducted a case-control study to explor...

journal_title：Journal of clinical laboratory analysis

authors： Tang W,Xu H,Ma D,Ma R,Wu J,Yu X,Feng J,Liu Q

更新日期：2020-03-01 00:00:00

abstract：:Urinary proteins from six patients with esophageal cancer and two with stomach cancer were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analyses were performed on days-1 to 3, 5, 7, 10, 14, and 21 (or 22) after surgery. The protein patterns were scanned by densitometry and divided ...

journal_title：Journal of clinical laboratory analysis

authors： Yokomizo K,Iijima S,Sakai N,Kurihara Y,Hitratsuka N,Nagai K,Iwai T,Shiba K

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:Gastrin-17 (G-17) and Helicobacter pylori (H pylori) antibody are widely used in the screening of gastric diseases, especially in gastric cancer. In this study, we aimed to evaluate the value of G-17 and H pylori antibody in gastric disease screening. METHODS:Healthy males and females (1368 and 1212, respec...

journal_title：Journal of clinical laboratory analysis

authors： Liu W,Sun Y,Yuan Y

更新日期：2020-08-30 00:00:00

abstract：:Early monitoring of HCVRNA during interferon treatment may allow clinicians to obtain important information that could help them to adopt therapeutic decisions in individual cases. The hepatitis C virus infection is highly dynamic and a daily high dose of IFN may induce a decline of viremia of 95+/-10% of baseline val...

journal_title：Journal of clinical laboratory analysis

authors： Magalini A,Puoti M,Putzolu V,Quiros-Roldan E,Forleo MA,Rossi S,Zaltron S,Spinetti A,Zanini B,Zonaro A,Solfrini R,Carosi G

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:There is a high prevalence of Hepatitis B virus (HBV) infection in China. The aim of this article is to reveal the HBV prevalence and its outcome in southern China by analyzing the HBV serum markers and their relationship to HBV genotype. METHODS:HBV serum markers, HBV-DNA and Alanine aminotransferase (ALT)...

journal_title：Journal of clinical laboratory analysis

authors： Lin S,Liu C,Shang H,Chen H,Yang B,Chen J,Chen Y,Chen D,Ou Q

更新日期：2013-03-01 00:00:00

abstract：:The experimental and clinical effectiveness of nasal salmon calcitonin (SCT) for treatment of osteoporosis in humans has been well established, but none is known yet about the pharmacokinetic property in relation to therapeutic efficacy, especially when used in a therapeutic dose range. This preclinical study was desi...

journal_title：Journal of clinical laboratory analysis

authors： Nakamuta H,Kohno T,Ichikawa M,Hoshino T,Watabe K,Koida M

更新日期：1997-01-01 00:00:00

abstract：:Vitamin D plays a vital role not only in bone health but also in pathophysiology of many other body functions. In recent years, there has been significant increase in testing of 25-hydroxyvitamin D (25-OH vitamin D), a marker of vitamin D deficiency. The most commonly used methods for the measurement of 25-OH vitamin ...

journal_title：Journal of clinical laboratory analysis

authors： Garg U,Munar A,Frazee C 3rd,Scott D

更新日期：2012-09-01 00:00:00

abstract：BACKGROUND:Standards play an important role in detection of the BCR-ABL1 fusion gene (FG) transcript. However, the standards widely used in laboratories are mainly based on plasmids or cDNA, which cannot accurately reflect the process of RNA extraction and cDNA synthesis. Therefore, we aimed to develop armored RNA-base...

journal_title：Journal of clinical laboratory analysis

authors： Fu Y,Zhang R,Wu Q,Zhang J,Bao L,Li J

更新日期：2018-11-01 00:00:00

abstract：:The proteoglycan decorin inhibits TGF-beta; therefore, it could antagonize progression of fibrotic diseases associated with activation of TGF-beta(1). The effect of decorin transfection in human mesangial cells (HMCs) on the expression of genes related to kidney fibrosis was investigated. HMCs, isolated from glomeruli...

journal_title：Journal of clinical laboratory analysis

authors： Costacurta A,Priante G,D'Angelo A,Chieco-Bianchi L,Cantaro S

更新日期：2002-01-01 00:00:00

abstract：BACKGROUND:After hematopoietic stem cell transplantation (HSCT), early detection of engraftment would be of critical value for clinicians. The aim of this study was to identify faster parameters for engraftment. METHODS:We evaluated blood cell parameters including complete blood count (CBC), differential counts, and v...

journal_title：Journal of clinical laboratory analysis

authors： Yahng SA,Lee JW,Kim Y,Kim M,Oh EJ,Park YJ,Lee JW,Cho B,Han K

更新日期：2014-11-01 00:00:00

abstract：BACKGROUND:The aim of this study was to investigate the differential plasma levels of lipocalin 2 (LCN2) and its complex with MMP-9 (where MMP is matrix metalloproteinase) before and after antibiotic treatment in hospitalized adult patients with community-acquired pneumonia (CAP). METHOD:Plasma LCN2 and LCN2/MMP-9 com...

journal_title：Journal of clinical laboratory analysis

authors： Yeh YH,Chang JL,Hsiao PC,Tsao SM,Lin CH,Kao SJ,Chou MC,Yang SF,Chien MH

更新日期：2013-07-01 00:00:00

abstract：BACKGROUND:The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high-quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM ...

journal_title：Journal of clinical laboratory analysis

authors： Sampedro A,Rodríguez-Granger J,Gómez C,Lara A,Gutierrez J,Otero A

更新日期：2013-11-01 00:00:00

abstract：:The present study was designed to evaluate whether the use of acid citrate dextrose (ACD) Formula A may enhance the survival of platelets during storage, thus allowing the continuance of platelet studies over the period of 2-3 hours usually recommended. For this purpose the effects of time on in vitro platelet respons...

journal_title：Journal of clinical laboratory analysis

authors： Pignatelli P,Pulcinelli FM,Ciatti F,Pesciotti M,Ferroni P,Gazzaniga PP

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:Osteoarthritis (OA) is a popular arthrosis featured as pain, limited joint activity, and deformity. Cyclooxygenase-2 (COX-2) has been reported to be up-regulated in arthritic tissues and is integral to the progression of osteoarthritis (OA). Previous studies showed the COX-2 promoter G-765C polymorphism coul...

journal_title：Journal of clinical laboratory analysis

authors： Huang W,Deng C,Tian F,Gao W,Ding Z,Rao X,Yin Z

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:MYBPC3 mutations have been described in dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM). A mutation, c.3373G>A, has been reported to cause autosomal recessive form of HCM. Here, we report that this mutation can cause autosomal dominant form of DCM. METHODS:Next-generation sequencing using...

journal_title：Journal of clinical laboratory analysis

authors： Mahdieh N,Hosseini Moghaddam M,Motavaf M,Rabbani A,Soveizi M,Maleki M,Rabbani B,Alizadeh-Asl A

更新日期：2018-07-01 00:00:00

abstract：:The levels of serum IgA and C3 in patients with IgA nephropathy were determined using international standard serum (IFCC/CRM470) in a multicenter trial in Japan. The ratio of serum IgA to C3 (serum IgA/C3 ratio) without any information from renal biopsy was used for the diagnosis of IgA nephropathy. Three hundred and ...

journal_title：Journal of clinical laboratory analysis

authors： Tomino Y,Suzuki S,Imai H,Saito T,Kawamura T,Yorioka N,Harada T,Yasumoto Y,Kida H,Kobayashi Y,Endoh M,Sato H,Saito K

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:Early recognition and treatment for severe drug eruption are important in improving clinical outcomes. A few studies have reported laboratory parameters to evaluate the severity of drug eruptions. This study aimed to determine the association between serum ferritin and the severity of drug eruptions. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Pan Y,Tang S,Zheng S,Li S,Zhu D,Fang H,Qiao J

更新日期：2020-02-01 00:00:00

abstract：BACKGROUND:Little is known about the involvement of herpes simplex virus (HSV) or Mycobacterium tuberculosis (MTB) as potentially curable causes of central nervous system (CNS) infections in sub-Saharan Africa. OBJECTIVE:In this study, we developed a PCR assay dedicated to simultaneous testing of HSV1/HSV2 and MTB in ...

journal_title：Journal of clinical laboratory analysis

authors： Zida S,Kolia-Diafouka P,Kania D,Sotto A,Foulongne V,Bolloré K,Ouangraoua S,Méda N,Carrère-Kremer S,Van de Perre P,Tuaillon E

更新日期：2019-03-01 00:00:00