Decrease of HCVRNA after three days of daily interferon treatment is predictive of the virological response at one month.

abstract：BACKGROUND:Although it is widely acknowledged that physical activity confers several health benefits, it remains uncertain whether strenuous and physically demanding exercise might determine biological effects that might turn to be ultimately unfavorable for health. Copeptin and midregion proadrenomedullin (MR-proADM) ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Schena F,Salvagno GL,Sanchis-Gomar F,Guidi GC

更新日期：2015-01-01 00:00:00

abstract：OBJECTIVE:To explore the clinical value of serum IgM and IgG to SARS-CoV-2 in COVID-19. METHODS:105 COVID-19 patients were enrolled as the disease group. 197 non-COVID-19 patients served as the control group. Magnetic chemiluminescent immunoassay (MCLIA) was used to detect the IgM and IgG. RESULTS:The peak of positiv...

journal_title：Journal of clinical laboratory analysis

authors： Wu LX,Wang H,Gou D,Fu G,Wang J,Guo BQ

更新日期：2020-11-13 00:00:00

abstract：BACKGROUND:Toxocariasis is the clinical term that is applied to infection in the human host with Toxocara species larvae. Serological tests are important tools for the diagnosis of toxocariasis. The aim of this study was to evaluate the excretory-secretory (ES) antigens of T. cati larvae using enzyme-linked immunosorbe...

journal_title：Journal of clinical laboratory analysis

authors： Zibaei M,Sadjjadi SM,Sarkari B,Uga S

更新日期：2016-05-01 00:00:00

abstract：:We described an automated enzymatic assay for conjugated bilirubin (Bc) in serum using the Iatro D-Bil kit, with bilirubin oxidase (EC 1.3.3.5 BOD) from Myrothecium species. The specificity of the enzyme in the Iatro D-Bil kit was examined by analyzing unconjugated bilirubin (Bu), delta bilirubin (Bdelta), and Bc with...

journal_title：Journal of clinical laboratory analysis

authors： Kimura S,Iyama S,Yamaguchi Y,Hayashi S,Yanagihara T

更新日期：1999-01-01 00:00:00

abstract：:The Venereal Disease Research Laboratory (VDRL) test has long been considered the best serological test for the diagnosis of neurosyphilis. The goal of this study was to find out if the Rapid Plasma Reagin (RPR) could be an alternative to the VDRL. Cerebrospinal fluid (CSF) and sera samples from patients in the follow...

journal_title：Journal of clinical laboratory analysis

authors： Castro R,Prieto ES,da Luz Martins Pereira F

更新日期：2008-01-01 00:00:00

abstract：:Amyloid peptide is thought to play a critical role in neuronal death in Alzheimer's disease (AD), most likely through oxidative stress. Free radical-related injury leads to DNA breaks, which subsequently activates the repair enzyme poly(ADP-ribose) polymerase-1 (PARP-1). In this study, the relationship between genetic...

journal_title：Journal of clinical laboratory analysis

authors： Liu HP,Lin WY,Wu BT,Liu SH,Wang WF,Tsai CH,Lee CC,Tsai FJ

更新日期：2010-01-01 00:00:00

abstract：:A novel and sensitive noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for alpha-human atrial natriuretic peptide (alpha-hANP) in plasma, which uses only one monoclonal IgG for the ring structure of alpha-hANP, is described. Plasma was filtered through polysaccharide membrane to separate peptides...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Yamamoto N,Ishikawa E

更新日期：1991-01-01 00:00:00

abstract：BACKGROUND:Gastrin-17 (G-17) and Helicobacter pylori (H pylori) antibody are widely used in the screening of gastric diseases, especially in gastric cancer. In this study, we aimed to evaluate the value of G-17 and H pylori antibody in gastric disease screening. METHODS:Healthy males and females (1368 and 1212, respec...

journal_title：Journal of clinical laboratory analysis

authors： Liu W,Sun Y,Yuan Y

更新日期：2020-08-30 00:00:00

abstract：BACKGROUND:It has been identified that incidence of infertility was about 20% among couples worldwide, about 50% caused by male elements. However, conventional semen laboratory detections could not handle clinical needs, which led to more comprehensive parameters for male fertility evaluation. We aimed to investigate t...

journal_title：Journal of clinical laboratory analysis

authors： Lu R,Chen X,Yu W,Jiang F,Zhou X,Xu Y,Wang F

更新日期：2020-09-14 00:00:00

abstract：BACKGROUND:The Architect HIV Ag/Ab Combo has excellent performance for HIV screening; however, the false-positive rate (FPR) was high in low HIV prevalence setting. OBJECTIVES:The purpose of this study was to analyze the influence of sample-to-cutoff (s/co) ratios by Architect HIV Ag/Ab Combo with the results of confi...

journal_title：Journal of clinical laboratory analysis

authors： Wang L,Wang JY,Tian XD,Ruan JX,Yu Y,Yan F

更新日期：2019-06-01 00:00:00

abstract：BACKGROUND:Technical innovation of autoimmune blistering dermatoses (ABDs) diagnosis aimed at multiplex approach. Two multiparametric ELISA tests are commercially available for ABDs serology. The aim was to compare diagnostic accuracy of multiparametric and monospecific ELISAs and to examine the diagnostic value/agreem...

journal_title：Journal of clinical laboratory analysis

authors： Gornowicz-Porowska J,Seraszek-Jaros A,Bowszyc-Dmochowska M,Bartkiewicz P,Kaczmarek E,Dmochowski M

更新日期：2018-05-01 00:00:00

abstract：BACKGROUND:The HER2 (human epidermal growth factor receptor-2) Ile655Val (rs1136201) genetic polymorphism can alter the receptor structure and its auto-activation, which can modify the signal transduction and, consequently, the cell cycle regulation. For this reason, this polymorphism has been extensively investigated ...

journal_title：Journal of clinical laboratory analysis

authors： de Almeida FC,Banin Hirata BK,Ariza CB,Losi Guembarovski R,de Oliveira KB,Suzuki KM,Guembarovski AL,Oda JMM,Vitiello GAF,Watanabe MAE

更新日期：2018-07-01 00:00:00

abstract：BACKGROUND:The pulmonary surfactant especially lipids in amniotic fluid can reflect the development stage of fetal lung maturity (FLM). However, the conventional lecithin/sphingomyelin (L/S) ratio method by thin layer chromatography (TLC) is insufficient and inconvenient for FLM prediction in clinical practice. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Cao Z,Liu J,Xie X,Zhan S,Song W,Wu S,Sun Z,Dong Y,Tang G,Liu Y,Li L,Shen M,Zhai Y,Zou J,Liu X

更新日期：2020-04-01 00:00:00

abstract：OBJECTIVE:To develop a reliable and specific method for rapid prenatal diagnosis of Trisomy 21 (Down syndrome). METHODS:We established a dual color competitive fluorescent Polymerase Chain Reaction (PCR) to measure the gene dosage of Down syndrome critical region (DSCR), a single copy sequence in chromosome 21. Anothe...

journal_title：Journal of clinical laboratory analysis

authors： Wang Y,Zhang X,Ling B,He C,Xia Q,Chen F,Miyamori I,Yang Z,Fan C

更新日期：2013-05-01 00:00:00

abstract：:The detection of IgA and low-avidity IgG and antibodies in serum is a potentially useful marker of recent infection by a microorganism. We studied the reliability of IgG avidity and presence of IgA for the diagnosis of recent acute infection by rubella virus. Low-avidity IgG (Avy-EIA test) was determined with a modifi...

journal_title：Journal of clinical laboratory analysis

authors： Gutiérrez J,Rodríguez MJ,De Ory F,Piédrola G,Maroto MC

更新日期：1999-01-01 00:00:00

abstract：:Acid phosphatase was purified to electrophoretic homogeneity from human normal lung and spleen and was characterized biochemically and immunologically in comparison with prostate acid phosphatase (PAP). The apparent MW of lung acid phosphatase (LAP) and spleen acid phosphatase (SAP) was 110,000 and 100,000, respective...

journal_title：Journal of clinical laboratory analysis

authors： Lin MF,Li SS,Chu TM,Lee CL

更新日期：1990-01-01 00:00:00

abstract：:Ninety semen samples from male patients were studied. They were classified into 4 different groups; normospermic fertile, oligospermia, asthenospermia, and oligoasthenospermia. In addition to routine semen analysis, the hypo-osmotic swelling test was done for all these samples, measuring total protein and specific gra...

journal_title：Journal of clinical laboratory analysis

authors： al-Mogazy HM,al-Khadra S,Kenawy G,al-Zeftawy A,Mostafa T,Attia A,Attia AM

更新日期：1993-01-01 00:00:00

abstract：:An automated measurement of total and free hydroxyproline in serum or urine is presented that uses flow injection analysis. After exclusion of nonspecific substances, hydroxyproline was oxidized by chloramine-T and L-cysteine with Ehrlich's reagent. The linearity obtained was from 3.8 mumole/L to 1.22 mmole/L with goo...

journal_title：Journal of clinical laboratory analysis

authors： Uji Y,Karmen A,Okabe H,Hata K,Miura M,Ozaki K,Minamizaki M,Shibata T,Inayama S

更新日期：1994-01-01 00:00:00

abstract：:Congenital disorders of glycosylation are a rapidly growing group of inherited (neuro)metabolic disorders characterized by defects in glycosylation of proteins and lipids. This study discusses an analytical problem in the differentiation between hypoglycosylation and transferrin (Tf) protein variants. Analysis of seru...

journal_title：Journal of clinical laboratory analysis

authors： Marklová E,Albahri Z

更新日期：2009-01-01 00:00:00

abstract：BACKGROUND:To determine the metabolic characteristics of patients with colon cancer (CC) and rectal cancer (RC) using gas chromatography-mass spectrometry (GC-MS)-based metabolomics. METHODS:In this study, serum samples were collected from 22 CC patients and 23 RC patients preoperatively and postoperatively and 45 hea...

journal_title：Journal of clinical laboratory analysis

authors： Wu J,Wu M,Wu Q

更新日期：2020-08-01 00:00:00

abstract：:Previously, an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for p24 antigen of HIV-1 was developed. The immune complex comprising 2,4-dinitrophenyl-biotinyl-bovine serum albumin-rabbit anti-p24 Fab' conjugate, p24 antigen, and rabbit anti-p24 Fab' -beta-D-galactosidase conjugate was t...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Hashinaka K,Nishikata I,Saito A,Takamizawa A,Shinagawa H,Ishikawa E

更新日期：1996-01-01 00:00:00

abstract：:Didemnin A was conjugated at the amino terminus of the N-methylleucine residue, via the linkers N-succinimidyl-3-(2-pyridyldithio)-propionate and trans-1,4-maleimidomethyl-cyclohexane carboxylic acid, to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA). The didemnin-KLH conjugates were used to hyperimmun...

journal_title：Journal of clinical laboratory analysis

authors： Raybould TJ,Grothaus PG,Simpson SB,Bignami GS,Lazo CB,Newman RA

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the plasma long non-coding RNA (lncRNA) expression profile in knee osteoarthritis (KOA) patients and the value of candidate lncRNAs for predicting KOA risk. METHODS:Plasma was obtained for RNA sequencing (RNA-seq) in eight KOA patients and eight healthy controls (Ctrls). Ten ...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Li M,Zhang B,Zhang N,Feng Q

更新日期：2020-09-01 00:00:00

abstract：BACKGROUND:This study was performed to investigate the epidemiology of enterovirus (EV) infections in children at a single hospital during recent 5 years. This study was preformed from December 2008 to November 2013. METHODS:We subjected 1,067 cerebrospinal fluid (CSF) specimens from 1,053 patients and 678 stool speci...

journal_title：Journal of clinical laboratory analysis

authors： Jeon JS,Kim JK,Yu J,Kim JW

更新日期：2016-03-01 00:00:00

abstract：BACKGROUND:Functions, morphology, distributions, and index of the circulating cells are the most useful parameters that indicate various inflammatory and toxic conditions. The aim of this study was to investigate the clinical significance of these parameters in patients diagnosed with (synthetic) cannabis use disorder....

journal_title：Journal of clinical laboratory analysis

authors： Guzel D,Yazici AB,Yazici E,Erol A

更新日期：2017-11-01 00:00:00

abstract：:The present work describes modification of a widely used salting-out procedure to rapidly extract DNA suitable for PCR, using the ARMS method to amplify a target sequence in the beta-globin gene. The salting-out DNA extraction procedure did not completely remove or decrease the presence of inhibitors to PCR in a consi...

journal_title：Journal of clinical laboratory analysis

authors： Noguera NI,Tallano CE,Bragós IM,Milani AC

更新日期：2000-01-01 00:00:00

abstract：BACKGROUND:There is a high prevalence of Hepatitis B virus (HBV) infection in China. The aim of this article is to reveal the HBV prevalence and its outcome in southern China by analyzing the HBV serum markers and their relationship to HBV genotype. METHODS:HBV serum markers, HBV-DNA and Alanine aminotransferase (ALT)...

journal_title：Journal of clinical laboratory analysis

authors： Lin S,Liu C,Shang H,Chen H,Yang B,Chen J,Chen Y,Chen D,Ou Q

更新日期：2013-03-01 00:00:00

abstract：:Clinicians recognize and compensate for limitations in estimating the glomerular filtration rate (GFR) using serum creatinine (sCr) measurements by the use of timed collections and mathematical manipulations of sCr. These limitations stem from that fact that sCr is affected by nonrenal influences, including muscle mas...

journal_title：Journal of clinical laboratory analysis

authors： Massey D

更新日期：2004-01-01 00:00:00

abstract：:A sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for (anti-human T-cell leukemia virus type I) IgG (anti-HTLV-I IgG) in serum using recombinant gag p24(14-214) of HTLV-I is described. The recombinant gag p24(14-214) is soluble in the absence of detergents and allows the use of enzymes other ...

journal_title：Journal of clinical laboratory analysis

authors： Kohno T,Hirota K,Sakoda I,Yamasaki M,Yokoo Y,Ishikawa E

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:Iron overload is a major complication in patients with hemoglobin H (Hb H) disease and causes damage of tissues. METHODS:We investigated 26 Hb H patients and 75 controls to evaluate their oxidative stress and antioxidant statuses. RESULTS:There were significantly increased levels of superoxide anion in leu...

journal_title：Journal of clinical laboratory analysis

authors： Chiou SS,Tsao CJ,Tsai SM,Wu YR,Liao YM,Lin PC,Tsai LY

更新日期：2014-07-01 00:00:00