Comparative evaluation of a new chemiluminiscent assay and an ELISA for the detection of IgM against measles.

abstract：BACKGROUND:Although it is widely acknowledged that physical activity confers several health benefits, it remains uncertain whether strenuous and physically demanding exercise might determine biological effects that might turn to be ultimately unfavorable for health. Copeptin and midregion proadrenomedullin (MR-proADM) ...

journal_title：Journal of clinical laboratory analysis

authors： Lippi G,Schena F,Salvagno GL,Sanchis-Gomar F,Guidi GC

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:The study evaluated the impact of interferences on the analytical specificity of three commercial and commonly used creatinine methods (two Jaffe and one enzymatic). METHODS:Manufacturer creatinine methods plus modified methods were tested with the following interferences: spiking serum with bilirubin, albu...

journal_title：Journal of clinical laboratory analysis

authors： Srisawasdi P,Chaichanajarernkul U,Teerakanjana N,Vanavanan S,Kroll MH

更新日期：2010-01-01 00:00:00

abstract：:Rare abnormal haemoglobin (Hb) often poses a problem in the diagnosis of relative electrophoresis mobility. The rare neutral Hbs interact with the Hb S and thus can increase the severity of sickle cell anaemia. In the present study, we investigated the use of reversed-phase high-performance liquid chromatography (RP-H...

journal_title：Journal of clinical laboratory analysis

authors： Yapo PA,Datté JY,Yapo A,Wachman H

更新日期：2004-01-01 00:00:00

abstract：:The objective of this study was to analyze apparently discrepant results that arose during the use of an indirect immunofluorescence (IIF) assay using transfected HEp-2 cells to detect anti-SS-A/Ro autoantibodies in human sera. Fourteen sera that had SS-A/Ro antibodies as detected on this commercial substrate, but did...

journal_title：Journal of clinical laboratory analysis

authors： Fritzler MJ,Hanson C,Miller J,Eystathioy T

更新日期：2002-01-01 00:00:00

abstract：:Epithelial cells are typically connected through different types of cell junctions that are localized from the apical membrane to the basal surface. In this way, epithelium cells form the first barrier against pathogenic microorganisms and prevent their entry into internal organs and the circulatory system. Recent stu...

journal_title：Journal of clinical laboratory analysis

authors： Zheng M,Sun S,Zhou J,Liu M

更新日期：2020-10-17 00:00:00

abstract：:It is well established that the targeted receptor for ciguatoxin (CTX) in mammalian tissues is the sodium channel, affecting the influx of sodium into cells and altering the action potential and function of the cell. Since the syntheses of fragments of CTX has become available, our focus has been on the receptor funct...

journal_title：Journal of clinical laboratory analysis

authors： Hokama Y,Chun KE,Campora CE,Higa N,Suma C,Hamajima A,Isobe M

更新日期：2006-01-01 00:00:00

abstract：:One of the most significant achievements of the biochemist during the past two decades is the use to which immunologically based assays have been put in clinical diagnosis (Hood et al.: Immunology, 1984). The problem faced and surmounted by immunologists in effecting the transition from research tool to routine clinic...

journal_title：Journal of clinical laboratory analysis

authors： Nicholls PJ,Malcolm AD

更新日期：1989-01-01 00:00:00

abstract：:N-terminal pro-brain natriuretic peptide (NT-proBNP) and BNP measurement could have a significant role in differentiating dyspnea between cardiac or pulmonary origin in the emergency room. The development of new and different commercial assays for these B-type natriuretic peptides offers the possibility of improving a...

journal_title：Journal of clinical laboratory analysis

authors： Sanz MP,Borque L,Rus A,Vicente B,Ramírez Y,Lasa L

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:After hematopoietic stem cell transplantation (HSCT), early detection of engraftment would be of critical value for clinicians. The aim of this study was to identify faster parameters for engraftment. METHODS:We evaluated blood cell parameters including complete blood count (CBC), differential counts, and v...

journal_title：Journal of clinical laboratory analysis

authors： Yahng SA,Lee JW,Kim Y,Kim M,Oh EJ,Park YJ,Lee JW,Cho B,Han K

更新日期：2014-11-01 00:00:00

abstract：:Novel molecular approaches have recently become available allowing improved major histocompatibility complex (MHC) matching of potential allogeneic bone marrow donors and recipients. Current cellular and serological assays are hindered by aberrant cell populations and limited reagents which only detect an individuals'...

journal_title：Journal of clinical laboratory analysis

authors： Rubocki RJ,Wisecarver JL,Hook DD,Cox SM,Beisel KW

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the plasma long non-coding RNA (lncRNA) expression profile in knee osteoarthritis (KOA) patients and the value of candidate lncRNAs for predicting KOA risk. METHODS:Plasma was obtained for RNA sequencing (RNA-seq) in eight KOA patients and eight healthy controls (Ctrls). Ten ...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Li M,Zhang B,Zhang N,Feng Q

更新日期：2020-09-01 00:00:00

abstract：:To investigate the influence of race, cigarette smoking, and immunologic parameters on serum immunoglobulins, we analyzed serum IgG, IgA, and IgM levels in 455 healthy adults. The study population ranged in age from 20 to 69 years, including 282 whites and 173 blacks, 181 never-smokers, 93 ex-smokers, and 181 current ...

journal_title：Journal of clinical laboratory analysis

authors： Tollerud DJ,Brown LM,Blattner WA,Weiss ST,Maloney EM,Kurman CC,Nelson DL,Hoover RN

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND:The aim of this study was to detect reliability of Brucella Coombs gel test (BCGT) by comparing with with ELISA (IgG + IgM), Standard agglutination test, and Brucella immunocapture agglutination methods in serological diagnosis of brucellosis. METHODS:Brucella Coombs gel test (BCGT), Brucella ELISA (IgG + I...

journal_title：Journal of clinical laboratory analysis

authors： Kalem F,Ergün AG,Durmaz S,Doğan M,Ertuğrul Ö,Gündem S

更新日期：2016-09-01 00:00:00

abstract：:Positive predictive values for the diagnosis of systemic lupus erythematosus (SLE) were determined for two antinative DNA assays: a Farr-type radioimmunoassay (RIA) and a solid-phase enzyme immunoassay (EIA). The study population consisted of all patients whose EIA anti-DNA results were positive (greater than 250 IU) ...

journal_title：Journal of clinical laboratory analysis

authors： Clough JD,Chang RK

更新日期：1989-01-01 00:00:00

abstract：OBJECTIVE:To develop a reliable and specific method for rapid prenatal diagnosis of Trisomy 21 (Down syndrome). METHODS:We established a dual color competitive fluorescent Polymerase Chain Reaction (PCR) to measure the gene dosage of Down syndrome critical region (DSCR), a single copy sequence in chromosome 21. Anothe...

journal_title：Journal of clinical laboratory analysis

authors： Wang Y,Zhang X,Ling B,He C,Xia Q,Chen F,Miyamori I,Yang Z,Fan C

更新日期：2013-05-01 00:00:00

abstract：:We studied antibody production in serial serum samples from patients with acute Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infections. Sera were analyzed both by enzyme-linked immunosorbent assay (ELISA) using a synthetic peptide (P62) derived from the glycine-alanine repeating region of the Epstein-Barr nucle...

journal_title：Journal of clinical laboratory analysis

authors： Rhodes G,Smith RS,Rubin RE,Vaughan J,Horwitz CA

更新日期：1990-01-01 00:00:00

abstract：:Determination of free digoxin levels in patients treated with digoxin immune FAb has long been a problematic area in clinical laboratory testing. The older radioimmunoassays resulted in inaccurate and variable results due to the competition of the administered drug with the radioactively labelled forms. The 1995 Physi...

journal_title：Journal of clinical laboratory analysis

authors： Honda SA,Rios CN,Murakami L,Morita T,Scottolini AG,Bhagavan NV

更新日期：1995-01-01 00:00:00

abstract：BACKGROUND:MicroRNAs play a vital role in coronary artery disease. Abnormal expression of microRNAs has been found to be associated with the occurrence of CAD. METHODS:We identified significantly differentially expressed microRNAs in plasma between 40 patients with CAD and 10 controls with NCA using RNA sequencing. Th...

journal_title：Journal of clinical laboratory analysis

authors： Zhong Z,Zhong W,Zhang Q,Zhang Q,Yu Z,Wu H

更新日期：2020-01-01 00:00:00

abstract：:A microparticle-enhanced nephelometric immunoassay was developed for lactoferrin quantitation in human milk. It is based on the nephelometric measurement of the light scattered during the competitive immuno-agglutination of a microparticle-lactoferrin conjugate with an antilactoferrin antiserum. This immunoassay is se...

journal_title：Journal of clinical laboratory analysis

authors： Cuillière ML,Montagne P,Molé C,Béné MC,Faure G

更新日期：1997-01-01 00:00:00

abstract：BACKGROUND:Neurofilaments are the major cytoskeletal components of neurons, and cell injury leads to their release into the surrounding area. The aim of this study was to compare the cerebrospinal fluid (CSF) and serum (S) concentrations of neurofilament light chains (NFLs) and phosphorylated neurofilament heavy chains...

journal_title：Journal of clinical laboratory analysis

authors： Kušnierová P,Zeman D,Hradílek P,Čábal M,Zapletalová O

更新日期：2019-09-01 00:00:00

abstract：OBJECTIVE:A common problem in clinical laboratories is maintaining the stability of analytes during pre-analytical processes. The aim of this study was to systematically summarize the results of a set of studies about the biochemical analytes stability. METHODS:A literature search was performed on the Advanced search ...

journal_title：Journal of clinical laboratory analysis

authors： Hedayati M,Razavi SA,Boroomand S,Kheradmand Kia S

更新日期：2020-09-01 00:00:00

abstract：BACKGROUND:This study was performed to compare the clinical efficacies of anterior cruciate ligament (ACL) reconstruction with autologous ligament grafting at different time points. METHODS:Eighty-five patients with ACL were categorized into two groups: Group A (GA, n = 45), who underwent early-stage (≤3 weeks) surger...

journal_title：Journal of clinical laboratory analysis

authors： Hu B,Gao F,Li C,Zhang B,An M,Lu M,Liu Y,Liu Y

更新日期：2020-08-26 00:00:00

abstract：:A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominantly GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols ...

journal_title：Journal of clinical laboratory analysis

authors： Scott RB,Collins JM,Matin S,White F,Swerdlow PS

更新日期：1990-01-01 00:00:00

abstract：OBJECTIVE:The coronavirus disease 2019 (COVID-19) has rapidly developed into a pandemic. Increased levels of ferritin due to cytokine storm and secondary hemophagocytic lymphohistiocytosis were found in severe COVID-19 patients. Therefore, the aim of this study was to determine the role of ferritin in COVID-19. METHOD...

journal_title：Journal of clinical laboratory analysis

authors： Cheng L,Li H,Li L,Liu C,Yan S,Chen H,Li Y

更新日期：2020-10-01 00:00:00

abstract：OBJECTIVE:Cell-free circular RNAs (circRNAs) are stable and abundantly exist in body fluids. In this study, we aimed to investigate plasma cell-free circRNAs as a novel class of biomarkers for the diagnosis of breast cancer (BC). METHODS:Differentially expressed circRNAs from 6 normal and 6 BC plasma samples were dete...

journal_title：Journal of clinical laboratory analysis

authors： Hu Y,Song Q,Zhao J,Ruan J,He F,Yang X,Yu X

更新日期：2020-09-01 00:00:00

abstract：:We studied a new proteinase K assay method for human serum mitochondrial aspartate aminotransferase. We found that proteinase K showed no inactivation of human mitochondrial aspartate aminotransferase isoenzyme and complete inactivation of cytosolic aspartate aminotransferase. Previous studies have shown that selectiv...

journal_title：Journal of clinical laboratory analysis

authors： Watazu Y,Uji Y,Okabe H,Shirahase Y,Kaneda N,Karmen A

更新日期：1993-01-01 00:00:00

abstract：:Mycobacterium tuberculosis bacilli are seldom demonstrated in tuberculous pleural effusion (TPE) by conventional bacteriological methods. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) was developed to detect IgG against four mycobacterial recombinant antigens (ESAT-6, PlcA, HspX and Tb8.4) in 69...

journal_title：Journal of clinical laboratory analysis

authors： Sumi S,Radhakrishnan VV

更新日期：2010-01-01 00:00:00

abstract：BACKGROUND:Sustaining proliferation is the most fundamental step for breast cancer tumor genesis. Accelerated proliferation is usually linked to the uncontrolled cell cycle. However, the internal and external factors linked to the activation of breast cancer cell cycle are still to be investigated. METHODS:quantitativ...

journal_title：Journal of clinical laboratory analysis

authors： Ye RY,Kuang XY,Zeng HJ,Shao N,Lin Y,Wang SM

更新日期：2020-08-01 00:00:00

abstract：BACKGROUND:Acute aortic dissection (AOD) is associated with high mortality and early diagnosis and treatment are essential. Ischemia-modified albumin (IMA) is a marker of myocardial ischemia whereas cardiac enzymes are released when myocardial necrosis occurs. We investigated, for the first time, whether IMA increases ...

journal_title：Journal of clinical laboratory analysis

authors： Sbarouni E,Georgiadou P,Marathias A,Panagiotakos D,Geroulanos S,Voudris V

更新日期：2010-01-01 00:00:00

abstract：OBJECTIVE:This study aimed to investigate the predictive value of microRNA-146b (miR-146b) on acute respiratory distress syndrome (ARDS) risk, and the correlation of miR-146b with disease severity and 28-day mortality in sepsis patients. METHODS:A total of 104 sepsis patients and 100 healthy controls (HCs) were consec...

journal_title：Journal of clinical laboratory analysis

authors： Chen W,Liu L,Yang J,Wang Y

更新日期：2020-08-26 00:00:00