Rapid and highly sensitive colloidal silver staining on cellulose acetate membrane for analysis of urinary proteins.

abstract：BACKGROUND:In this study, the fresh stool samples from 254 children under 5 years of age with acute gastroenteritis which were delivered between October 2012 and December 2013 were collected. METHODS:In the stool samples, rotavirus antigens were investigated using two different immunochromatographic methods which are ...

journal_title：Journal of clinical laboratory analysis

authors： Artiran S,Atalay A,Gökahmetoglu S,Ozturk MA,Balci N,Cakir N,Kilic H,Durmaz R

更新日期：2017-03-01 00:00:00

abstract：:The technique that allows for in situ detection of PCR-amplified DNA and cDNA has improved remarkably quickly over the past 5 years. As of this writing, one can expect good results after a relatively quick "learning curve." The technique demands some skill in working with glass slides and cells/tissues. Thus, I recomm...

journal_title：Journal of clinical laboratory analysis

authors： Nuovo GJ

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:Toxocariasis is the clinical term that is applied to infection in the human host with Toxocara species larvae. Serological tests are important tools for the diagnosis of toxocariasis. The aim of this study was to evaluate the excretory-secretory (ES) antigens of T. cati larvae using enzyme-linked immunosorbe...

journal_title：Journal of clinical laboratory analysis

authors： Zibaei M,Sadjjadi SM,Sarkari B,Uga S

更新日期：2016-05-01 00:00:00

abstract：BACKGROUND:The HER2 (human epidermal growth factor receptor-2) Ile655Val (rs1136201) genetic polymorphism can alter the receptor structure and its auto-activation, which can modify the signal transduction and, consequently, the cell cycle regulation. For this reason, this polymorphism has been extensively investigated ...

journal_title：Journal of clinical laboratory analysis

authors： de Almeida FC,Banin Hirata BK,Ariza CB,Losi Guembarovski R,de Oliveira KB,Suzuki KM,Guembarovski AL,Oda JMM,Vitiello GAF,Watanabe MAE

更新日期：2018-07-01 00:00:00

abstract：OBJECTIVE:To explore the role of serum periostin in patients with aneurysmal subarachnoid hemorrhage (aSAH). METHOD:We conducted a retrospective study and 124 aSAH patients treated in Shenzhen People's hospital during March 1st 2015 to December 30th 2016 were included. Baseline information, neurological status and cli...

journal_title：Journal of clinical laboratory analysis

authors： Luo W,Wang H,Hu J

更新日期：2018-06-01 00:00:00

abstract：:The aim of the present study was to investigate the serum total protein (TP), total sialic acid (TSA), lipid-associated sialic acid (LSA), LSA/TP, and LSA/TP values in type 2 diabetes mellitus (DM) patients. Two study groups (healthy controls and type 2 DM subjects) were examined. For the type 2 DM group, 120 patients...

journal_title：Journal of clinical laboratory analysis

authors： Ekin S,Meral I,Gunduz H,Mert N

更新日期：2003-01-01 00:00:00

abstract：BACKGROUND:Circular RNAs (circRNAs) are emerging noncoding RNAs that are involved in many biological processes and diseases. The expression profile of circRNAs in preterm neonates with bronchopulmonary dysplasia (BPD) remains unresolved. METHODS:In BPD infants, peripheral venous blood was drawn and circRNAs were extra...

journal_title：Journal of clinical laboratory analysis

authors： Mao X,Guo Y,Qiu J,Zhao L,Xu J,Yin J,Lu K,Zhang M,Cheng R

更新日期：2020-07-01 00:00:00

abstract：BACKGROUND:Alport syndrome (ATS) is a hereditary nephritis with hereditary and clinical heterogeneity; the early clinical symptoms are atypical, which can easily lead to misdiagnosis. The proband, a 6-year-old girl, was found to have microscopic hematuria, proteinuria, and visual impairment at about 5 years old; the re...

journal_title：Journal of clinical laboratory analysis

authors： Zhu Q,Zhou C,Wang J

更新日期：2020-09-06 00:00:00

abstract：BACKGROUND:This study aimed to evaluate the association of circular RNA La-related RNA-binding protein 4 (circ-LARP4) with clinical features and prognosis in osteosarcoma patients, and further explore its effect on chemosensitivity in osteosarcoma cells. METHODS:Seventy-two osteosarcoma patients with Enneking stage II...

journal_title：Journal of clinical laboratory analysis

authors： Hu Y,Gu J,Shen H,Shao T,Li S,Wang W,Yu Z

更新日期：2020-02-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the plasma long non-coding RNA (lncRNA) expression profile in knee osteoarthritis (KOA) patients and the value of candidate lncRNAs for predicting KOA risk. METHODS:Plasma was obtained for RNA sequencing (RNA-seq) in eight KOA patients and eight healthy controls (Ctrls). Ten ...

journal_title：Journal of clinical laboratory analysis

authors： Liu X,Li M,Zhang B,Zhang N,Feng Q

更新日期：2020-09-01 00:00:00

abstract：:Mycobacterium avium is an important intracellular pathogen, particularly in AIDS patients. It also shows the second frequency among nontuberculous mycobacteria infections in Korea. Point mutations of domain V region of the 23S rRNA gene has been known to confer clarithromycin resistance to M. avium. In order to isolat...

journal_title：Journal of clinical laboratory analysis

authors： Lee SH,Park YK,Kim CK,Kim HJ

更新日期：2011-01-01 00:00:00

abstract：OBJECTIVE:To investigate consistency of lymphocyte immunophenotype between labial gland and peripheral blood in patients with primary Sjogren's syndrome (pSS). METHODS:Seventy-one pSS patients and 35 patients with maxillofacial trauma were included in the study. Based on the ratio of CD20 to CD3 in labial gland from 7...

journal_title：Journal of clinical laboratory analysis

authors： Sun W,Zhang N,Zhang Y,Shao Z,Gong L,Wei W

更新日期：2018-11-01 00:00:00

abstract：:The shortcomings of current methods of basophil enumeration detract from the clinical value of the basophil count. Moreover, sophisticated and costly techniques of automated basophil counting hardly can be validated for lack of a suitable reference method. We investigated whether a flow cytometric technique using doub...

journal_title：Journal of clinical laboratory analysis

authors： Hübl W,Andert S,Erath A,Streicher J,Bayer PM

更新日期：1996-01-01 00:00:00

abstract：BACKGROUND:Cell-free DNA (cfDNA) in the plasma of patients with both malignant and benign breast lesions was analyzed to determine whether the findings may have diagnostic and prognostic implications and to analyze the association between the levels of cfDNA and prognostic parameters. METHODS:Plasma samples were obtai...

journal_title：Journal of clinical laboratory analysis

authors： Hashad D,Sorour A,Ghazal A,Talaat I

更新日期：2012-11-01 00:00:00

abstract：BACKGROUND:Renal failure is a severe complication of symptomatic myeloma, related to higher mortality. Recovery from dialysis dependence can lead to enormous survival benefits. We investigated the effect factors for probability of dialysis independence. METHODS:Retrospective data on 45 newly diagnosed MM (NDMM) patien...

journal_title：Journal of clinical laboratory analysis

authors： Song J,Jiang F,Liu H,Ding K,Ren Y,Li L,Wang G,Shao Z,Fu R

更新日期：2020-02-01 00:00:00

abstract：BACKGROUND:The aim of this study was to investigate the differential plasma levels of lipocalin 2 (LCN2) and its complex with MMP-9 (where MMP is matrix metalloproteinase) before and after antibiotic treatment in hospitalized adult patients with community-acquired pneumonia (CAP). METHOD:Plasma LCN2 and LCN2/MMP-9 com...

journal_title：Journal of clinical laboratory analysis

authors： Yeh YH,Chang JL,Hsiao PC,Tsao SM,Lin CH,Kao SJ,Chou MC,Yang SF,Chien MH

更新日期：2013-07-01 00:00:00

abstract：BACKGROUND:Iron overload is a major complication in patients with hemoglobin H (Hb H) disease and causes damage of tissues. METHODS:We investigated 26 Hb H patients and 75 controls to evaluate their oxidative stress and antioxidant statuses. RESULTS:There were significantly increased levels of superoxide anion in leu...

journal_title：Journal of clinical laboratory analysis

authors： Chiou SS,Tsao CJ,Tsai SM,Wu YR,Liao YM,Lin PC,Tsai LY

更新日期：2014-07-01 00:00:00

abstract：BACKGROUND:The aim of this study was to explain the effects of microRNA-132 in renal cell carcinoma by regulating FOXM1 expression. METHODS:Thirty patients with renal cell carcinoma admitted to our hospital were enrolled, and their adjacent normal tissues and cancer tissues were taken. The expression of microRNA-132 w...

journal_title：Journal of clinical laboratory analysis

authors： Yu Y,Lu W,Zhou X,Huang H,Shen S,Guo L

更新日期：2020-01-01 00:00:00

abstract：:The two serum proteins of the complement cascade in the highest concentrations, C3 and C4, respond to various conditions in much the same manner as do other positive acute-phase proteins. A major difference is that they are relatively sluggish in response to cytokine drive, requiring several days rather than hours to ...

journal_title：Journal of clinical laboratory analysis

authors： Ritchie RF,Palomaki GE,Neveux LM,Navolotskaia O,Ledue TB,Craig WY

更新日期：2004-01-01 00:00:00

abstract：BACKGROUND:MYBPC3 mutations have been described in dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM). A mutation, c.3373G>A, has been reported to cause autosomal recessive form of HCM. Here, we report that this mutation can cause autosomal dominant form of DCM. METHODS:Next-generation sequencing using...

journal_title：Journal of clinical laboratory analysis

authors： Mahdieh N,Hosseini Moghaddam M,Motavaf M,Rabbani A,Soveizi M,Maleki M,Rabbani B,Alizadeh-Asl A

更新日期：2018-07-01 00:00:00

abstract：:This study compared the use of conventional urine microscopy, the KOVA system, Neubauer hemacytometry, and the Yellow IRIS for the determination of white blood cell (WBC) and red blood cell (RBC) counts in urine samples. Both KOVA WBC and RBC counts correlated better with the IRIS counts than with conventional microsc...

journal_title：Journal of clinical laboratory analysis

authors： McGinley M,Wong LL,McBride JH,Rodgerson DO

更新日期：1992-01-01 00:00:00

abstract：BACKGROUND:Inflammation plays an important role in the development and progression of CRC. The members of inflammatory biomarkers, preoperative NLR and PLR, have been proved by numerous studies to be promising prognostic biomarkers for CRC. However, the diagnostic value of the two biomarkers in CRC remains unknown, and...

journal_title：Journal of clinical laboratory analysis

authors： Peng HX,Yang L,He BS,Pan YQ,Ying HQ,Sun HL,Lin K,Hu XX,Xu T,Wang SK

更新日期：2017-09-01 00:00:00

abstract：:Preeclampsia continues to be a major cause of maternal and perinatal mortality and morbidity worldwide. The etiopathogenesis of preeclampsia is not fully understood. Neopterin and interleukin-2 (IL-2) production reflects cellular immunity. Our purpose was to determine the levels of neopterin and interleukin-2 receptor...

journal_title：Journal of clinical laboratory analysis

authors： Kaleli I,Kaleli B,Demir M,Yildirim B,Cevahir N,Demir S

更新日期：2005-01-01 00:00:00

abstract：BACKGROUND:This study aimed to investigate the status of internal quality control (IQC) for cardiac biomarkers from 2011 to 2016 so that we can have overall knowledge of the precision level of measurements in China and set appropriate precision specifications. METHODS:Internal quality control data of cardiac biomarker...

journal_title：Journal of clinical laboratory analysis

authors： Li T,Wang W,Zhao H,He F,Zhong K,Yuan S,Wang Z

更新日期：2018-05-01 00:00:00

abstract：BACKGROUND:The association between genetic variations of vascular endothelial growth factor (VEGF) gene and the risk for atherosclerosis has been hypothesized. We aimed to assess the relationship between rs2010963 (+405 C/G) polymorphism and presence, severity, and outcome of coronary artery disease (CAD) in an Iranian...

journal_title：Journal of clinical laboratory analysis

authors： Kalayi Nia S,Ziaee S,Boroumand MA,Sotudeh Anvari M,Pourgholi L,Jalali A

更新日期：2017-07-01 00:00:00

abstract：:A method that could be facilitated for the quantitation and qualitation of gangliosides in human plasma was developed and applied in the present study for characterization of ganglioside patterns in plasmas of patients with neoplastic diseases including gastric cancer, adult T-cell leukemia (ATL), and acute lymphocyti...

journal_title：Journal of clinical laboratory analysis

authors： Ayabe M,Shichijo S,Yokoyama MM

更新日期：1989-01-01 00:00:00

abstract：:C-peptide is secreted in equimolar concentrations with insulin, and is often measured to assess pancreatic beta-cell function. C-peptide analysis is most often performed by radioimmunoassay (RIA) which has several disadvantages. We evaluated an automated, chemiluminescent immunoassay for C-peptide in terms of precisio...

journal_title：Journal of clinical laboratory analysis

authors： Hardy RW,Cohn M,Konrad RJ

更新日期：2000-01-01 00:00:00

abstract：:We described an automated enzymatic assay for conjugated bilirubin (Bc) in serum using the Iatro D-Bil kit, with bilirubin oxidase (EC 1.3.3.5 BOD) from Myrothecium species. The specificity of the enzyme in the Iatro D-Bil kit was examined by analyzing unconjugated bilirubin (Bu), delta bilirubin (Bdelta), and Bc with...

journal_title：Journal of clinical laboratory analysis

authors： Kimura S,Iyama S,Yamaguchi Y,Hayashi S,Yanagihara T

更新日期：1999-01-01 00:00:00

abstract：OBJECTIVE:A common problem in clinical laboratories is maintaining the stability of analytes during pre-analytical processes. The aim of this study was to systematically summarize the results of a set of studies about the biochemical analytes stability. METHODS:A literature search was performed on the Advanced search ...

journal_title：Journal of clinical laboratory analysis

authors： Hedayati M,Razavi SA,Boroomand S,Kheradmand Kia S

更新日期：2020-09-01 00:00:00

abstract：BACKGROUND:Red cell distribution width (RDW) and mean platelet volume (MPV) are considered to be associated with tumors. We investigated the diagnostic value of RDW, MPV, and cancer antigen 125 (CA125), alone or in combination, in the diagnosis of endometrial cancer and endometrial hyperplasia. METHODS:This study incl...

journal_title：Journal of clinical laboratory analysis

authors： Zhang H,Liang K,Ke L,Tang S

更新日期：2020-08-01 00:00:00