Abstract：
BACKGROUND:The HER2 (human epidermal growth factor receptor-2) Ile655Val (rs1136201) genetic polymorphism can alter the receptor structure and its auto-activation, which can modify the signal transduction and, consequently, the cell cycle regulation. For this reason, this polymorphism has been extensively investigated as a candidate marker for breast cancer (BC). In this context, the aim of this study was to evaluate the possible influence of HER2 Ile655Val in BC susceptibility and prognostic factors in a Brazilian population. METHODS:Polymorphism genotype was assessed through RFLP-PCR in 107 BC patients with clinicopathological data available and in 150 women with no evidence of neoplasia and with no familial history of BC as control group. Association between this polymorphism and BC susceptibility and clinical parameters was evaluated through odds ratio (OR) and chi-squared or Fisher's exact test, respectively. RESULTS:A significant negative association between valine allele and BC susceptibility in dominant model was found (OR 0.5; 95% CI 0.27-0.93, P = .036). No significant association was found in relation to BC clinicopathological features (tumor size, lymph nodes commitment, histological grade, HER2 overexpression, hormonal receptors, p53, and Ki-67). CONCLUSION:Although this polymorphism did not demonstrate potential as a prognostic marker, it may be a suitable susceptibility marker for BC.
journal_name
J Clin Lab Analjournal_title
Journal of clinical laboratory analysisauthors
de Almeida FC,Banin Hirata BK,Ariza CB,Losi Guembarovski R,de Oliveira KB,Suzuki KM,Guembarovski AL,Oda JMM,Vitiello GAF,Watanabe MAEdoi
10.1002/jcla.22406subject
Has Abstractpub_date
2018-07-01 00:00:00pages
e22406issue
6eissn
0887-8013issn
1098-2825journal_volume
32pub_type
杂志文章abstract：BACKGROUND:Multiple organ dysfunction syndromes (MODS) is reported as a leading cause of mortality in intensive care units. Recently, continuous blood purification (CBP) has been mostly applied for MODS treatment. Thus, the purpose of this study was to investigate the effects of CBP on plasma phospholipid level in pati...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22411
更新日期：2018-07-01 00:00:00
abstract：:A novel and ultrasensitive noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay) for alpha-human atrial natriuretic peptide (alpha-hANP) in plasma is described. alpha-hANP was biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-alpha-hANP [6-28] IgG-coated polystyre...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860060407
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abstract：:Using a fully automated reticulocyte counter, the roles of the reticulocyte count with maturation in pancytopenia were evaluated. Different groups of pancytopenia including aplastic anemia, infiltrative marrow disorder, hypersplenism, and megaloblastic anemia were recruited. All patients had bone marrow examinations f...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860070612
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND:N-terminal pro-Brain natriuretic peptide (NT-proBNP) is an important biomarker to indicate cardiac function, but its significance in cardiac surgery is still unclear. OBJECTIVE:To explore clinical significance of serum NT-proBNP and their dynamic characteristics in patients with cardiac valve replacement (C...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20435
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abstract：:Congenital hypoplastic anemias are a rare and heterogeneous group of disorders. This paper reviews new molecular diagnostic tests in two distinct forms of congenital anemias, anemia due to transplacental infection with B19 parvovirus and Fanconi anemia. In both instances, molecular assays making use of amplification o...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章,评审
doi：
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:In this study, the fresh stool samples from 254 children under 5 years of age with acute gastroenteritis which were delivered between October 2012 and December 2013 were collected. METHODS:In the stool samples, rotavirus antigens were investigated using two different immunochromatographic methods which are ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22030
更新日期：2017-03-01 00:00:00
abstract：:In 23 patients with common migraine (CM), immune responsiveness and frequency of immunocompetent cells were investigated. In particular, phagocytosis and killing of Candida albicans by polymorphs (PMNs) and monocytes were analyzed. Also, the percentages of CD3+, CD4+, CD8+, natural killer, and CD15+ cells were evaluat...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860040105
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND:Sustaining proliferation is the most fundamental step for breast cancer tumor genesis. Accelerated proliferation is usually linked to the uncontrolled cell cycle. However, the internal and external factors linked to the activation of breast cancer cell cycle are still to be investigated. METHODS:quantitativ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23315
更新日期：2020-08-01 00:00:00
abstract：OBJECTIVE:To explore the clinical value of serum IgM and IgG to SARS-CoV-2 in COVID-19. METHODS:105 COVID-19 patients were enrolled as the disease group. 197 non-COVID-19 patients served as the control group. Magnetic chemiluminescent immunoassay (MCLIA) was used to detect the IgM and IgG. RESULTS:The peak of positiv...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23649
更新日期：2020-11-13 00:00:00
abstract：BACKGROUNDS:Shortening of primary cilia in kidney epithelial cells is associated with kidney injury and involved with the induced level of α-tubulin in urine. Therefore, rapid detection and quantification of α-tubulin in the urine samples could be used to the preliminary diagnosis of kidney injury. METHODS:Cellulose-b...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23015
更新日期：2020-01-01 00:00:00
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journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23414
更新日期：2020-09-01 00:00:00
abstract：:Elevated serum chromogranin A (CgA) levels have been detected in patients with prostate cancer who have developed resistance to hormonal therapy. We would like to reexamine these cases by using serial specimens to determine whether such elevated levels are also detectable in prostate cancer patients not undergoing hor...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：BACKGROUND:Coronary heart disease (CHD) is primarily caused by atherosclerosis of coronary arteries. It is largely an inflammatory disease of the vascular wall. The inflammation is related to DNA methylation. Angiopoietin-like protein 2 (ANGPTL2) has various functions in several chronic inflammatory diseases. Macrophag...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22702
更新日期：2019-03-01 00:00:00
abstract：:Our objectives in this study were to compare results obtained by an enzyme immunoassay (EIA) for F-actin antibody (FAA) immunoglobulin G (IgG) to those determined by an indirect fluorescent antibody (IFA) assay for smooth muscle antibody (SMA) IgG, and to determine the prevalence of FAA in patient sera having serologi...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20167
更新日期：2007-01-01 00:00:00
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journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22834
更新日期：2019-05-01 00:00:00
abstract：:A microparticle-enhanced nephelometric immunoassay was developed for plasminogen quantitation in human plasma. It is based on the nephelometric measurement of the light scattered by microparticle clusters formed during a sandwich reaction between plasminogen, microparticle--anti-plasminogen conjugate, and the free ant...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2825(1996)10:2<85::AID-JCLA5>3.
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND:Recently, several studies have investigated the relationship between Pre-miR-27a rs895819 polymorphism and risk of various cancers. However, the relationship between rs895819 and diffuse large B-cell lymphoma (DLBCL) has not been well known. METHODS:In this study, we conducted a case-control study to explor...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23088
更新日期：2020-03-01 00:00:00
abstract：OBJECTIVE:This present study aimed to investigate the correlation of circular RNA SMARCA5 (circ-SMARCA5) with clinicopathological features and overall survival (OS), and the effect of circ-SMARCA5 on cell proliferation and chemotherapy sensitivity to cisplatin/gemcitabine in intrahepatic cholangiocarcinoma (ICC). METH...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23138
更新日期：2020-04-01 00:00:00
abstract：BACKGROUND:Recent studies have shown that neutrophil lymphocyte ratio (NLR) is a strong indicator in determining inflammation in cardiac and non-cardiac diseases. We aimed to evaluate the relationship between proteinuria and NLR in chronic kidney disease (CKD) patients without diabetes mellitus (DM). METHODS:Between 2...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21715
更新日期：2014-11-01 00:00:00
abstract：:Urinary proteins from six patients with esophageal cancer and two with stomach cancer were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Analyses were performed on days-1 to 3, 5, 7, 10, 14, and 21 (or 22) after surgery. The protein patterns were scanned by densitometry and divided ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20087
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abstract：:Hematological parameters are affected by different factors that include age, sex, smoking, ethnicity, and environmental altitude. It has been justified that each population must establish its own normal reference intervals to be used in clinical assessments and interpretations. Hematological reference intervals for ad...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20265
更新日期：2008-01-01 00:00:00
abstract：:Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), ...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860050208
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abstract：:A radioactive polymerase chain reaction (PCR) method has been developed for the relative quantification of the human alpha-2 chain of type I collagen [hu alpha-2(I)] in cells. cDNAs generated by reverse transcription from the total pool of cytoplasmic RNA serve as a template for polymerase chain reaction amplification...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.1860050407
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND:Aberrant levels of circulating microRNAs (miRNAs) are potential biomarkers in papillary thyroid carcinoma (PTC) diagnosis and therapy. The aim of this study was to evaluate serum exosomal miR-29a expression as a non-invasive biomarker for PTC diagnosis and prognosis. METHODS:Quantitative reverse transcripti...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23560
更新日期：2020-12-23 00:00:00
abstract：:The Venereal Disease Research Laboratory (VDRL) test has long been considered the best serological test for the diagnosis of neurosyphilis. The goal of this study was to find out if the Rapid Plasma Reagin (RPR) could be an alternative to the VDRL. Cerebrospinal fluid (CSF) and sera samples from patients in the follow...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.20254
更新日期：2008-01-01 00:00:00
abstract：:The technique that allows for in situ detection of PCR-amplified DNA and cDNA has improved remarkably quickly over the past 5 years. As of this writing, one can expect good results after a relatively quick "learning curve." The technique demands some skill in working with glass slides and cells/tissues. Thus, I recomm...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章,评审
doi：10.1002/(SICI)1098-2825(1996)10:6<335::AID-JCLA5>3
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND:Previous studies have demonstrated that dysfunctional regulatory T cells (Tregs) may be associated with Graves' disease (GD). In this study, we evaluated four serum Treg-associated miRNAs (miR-210, miR-182, miR-155, and miR-146a) expressions and assessed the potential of serum miRNAs as biomarkers of GD. ME...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.22266
更新日期：2018-02-01 00:00:00
abstract：:The aim of this study was to evaluate anti-HEV antibody profiles in urine specimens in comparison to corresponding serum samples to assess the utility of urine as a clinical specimen. Paired serum and urine specimens from 71 hepatitis E patients, 33 non-E hepatitis patients, 63 patients with nonhepatic diseases, and 2...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.10027
更新日期：2002-01-01 00:00:00
abstract：OBJECTIVE:Cell-free circular RNAs (circRNAs) are stable and abundantly exist in body fluids. In this study, we aimed to investigate plasma cell-free circRNAs as a novel class of biomarkers for the diagnosis of breast cancer (BC). METHODS:Differentially expressed circRNAs from 6 normal and 6 BC plasma samples were dete...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.23393
更新日期：2020-09-01 00:00:00
abstract：BACKGROUND:Platelet-reactive antibodies lead to thrombocytopenia and bleeding disorders, and diverse assays are used for their detection. In this retrospective analysis, the applicability of three different test systems was compared and antibody specificities were assessed. METHODS:Sera of 1,234 patients were tested w...
journal_title：Journal of clinical laboratory analysis
pub_type： 杂志文章
doi：10.1002/jcla.21750
更新日期：2015-05-01 00:00:00
