Hepatitis E antibody profiles in serum and urine.

Abstract:

:The aim of this study was to evaluate anti-HEV antibody profiles in urine specimens in comparison to corresponding serum samples to assess the utility of urine as a clinical specimen. Paired serum and urine specimens from 71 hepatitis E patients, 33 non-E hepatitis patients, 63 patients with nonhepatic diseases, and 26 healthy individuals were tested by recombinant HEV protein (55 kD)-based indirect enzyme-linked immunosorbent assay (ELISA). Uronegativity for anti-HEV IgM was noted in 71 (100%) serologically confirmed patients with hepatitis E. Hepatitis E patients (10/10) showed urinary absence or very low levels of total IgM by capture ELISA, suggesting absence or low levels of filtration, and/or local synthesis, and/or transudation of IgM in urine during infection. When these patients were tested for total IgG and IgA, microquantities of immunoglobulins were noted in all urine samples (10/10 for each). However, the proportions of uropositivity for anti-HEV IgG and IgA in hepatitis E patients were low and indicated only 21.42% and 49.33% concordance with seropositivity, respectively. Control groups also showed low and variable uropositivity for anti-HEV IgG and IgA. Overall, HEV-specific antibodies exhibited by serum in recent and past infections were not found in urine. The study demonstrated the inadequacy of urine specimens for detection of hepatitis E antibodies.

journal_name

J Clin Lab Anal

authors

Joshi MS,Walimbe AM,Arankalle VA,Chadha MS,Chitambar SD

doi

10.1002/jcla.10027

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

137-42

issue

3

eissn

0887-8013

issn

1098-2825

journal_volume

16

pub_type

杂志文章
  • A landscape of circulating long non-coding RNA (lncRNA) expression profile and the predictive value of candidate lncRNAs for disease risk of knee osteoarthritis.

    abstract:BACKGROUND:This study aimed to investigate the plasma long non-coding RNA (lncRNA) expression profile in knee osteoarthritis (KOA) patients and the value of candidate lncRNAs for predicting KOA risk. METHODS:Plasma was obtained for RNA sequencing (RNA-seq) in eight KOA patients and eight healthy controls (Ctrls). Ten ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23423

    authors: Liu X,Li M,Zhang B,Zhang N,Feng Q

    更新日期:2020-09-01 00:00:00

  • High level of D-dimer predicts ischemic stroke in patients with infective endocarditis.

    abstract:BACKGROUND:Ischemic stroke is one of the most prominent and serious neurological complications of infective endocarditis (IE). Our study was designed to evaluate the predictive value of higher level of plasma D-dimer on admission for the development of ischemic stroke in patients with IE. METHODS:In this prospective s...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23206

    authors: Xu N,Fu Y,Wang S,Li S,Cai D

    更新日期:2020-05-01 00:00:00

  • A new blood collection device minimizes cellular DNA release during sample storage and shipping when compared to a standard device.

    abstract:BACKGROUND:Cell-free DNA (cfDNA) circulating in blood is currently used for noninvasive diagnostic and prognostic tests. Minimizing background DNA is vital for detection of low abundance cfDNA. We investigated whether a new blood collection device could reduce background levels of genomic DNA (gDNA) in plasma compared ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21603

    authors: Norton SE,Luna KK,Lechner JM,Qin J,Fernando MR

    更新日期:2013-07-01 00:00:00

  • Rapid and highly sensitive colloidal silver staining on cellulose acetate membrane for analysis of urinary proteins.

    abstract::In order to establish a rapid staining method sensitive enough to stain the protein bands on cellulose acetate membrane and suitable for the analysis of urinary proteins in healthy subjects, we conducted a detailed examination of Kovarik's method designed for the analysis of proteins in cerebrospinal fluid in detail. ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2825(1996)10:6<403::AID-JCLA15>

    authors: Hiratsuka N,Shiba K,Shinomura K,Hosaki S

    更新日期:1996-01-01 00:00:00

  • Validation of a free phenytoin assay on Cobas c501 analyzer using calibrators from Cobas Integra free phenytoin assay by comparing its performance with fluorescence polarization immunoassay for free phenytoin on the TDx analyzer.

    abstract::For many years, fluorescence polarization immunoassay (FPIA) on the TDx analyzer has been used for determination of free phenytoin concentration. Recently Abbott Laboratories decided to discontinue the TDx analyzer and related assays on this analyzer. Free phenytoin assay is also available from Roche Diagnostics for a...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21536

    authors: Dasgupta A,Davis B,Chow L

    更新日期:2013-01-01 00:00:00

  • fbl gene as a species-specific target for Staphylococcus lugdunensis identification.

    abstract::Staphylococcus lugdunensis is an unusually virulent coagulase-negative species, associated with severe infections. The present report describes the development of a single-step, species-specific PCR protocol for S. lugdunensis identification. fbl gene, encoding a fibrinogen-binding adhesin, was exploited and assessed ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20352

    authors: Chatzigeorgiou KS,Siafakas N,Petinaki E,Zerva L

    更新日期:2010-01-01 00:00:00

  • Continuous blood purification ameliorates clinical signs and corrects the plasma phospholipid levels of patients with multiple organ dysfunction syndromes.

    abstract:BACKGROUND:Multiple organ dysfunction syndromes (MODS) is reported as a leading cause of mortality in intensive care units. Recently, continuous blood purification (CBP) has been mostly applied for MODS treatment. Thus, the purpose of this study was to investigate the effects of CBP on plasma phospholipid level in pati...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22411

    authors: Wen W,Wang K,Jiang ZM,Zhang ZH,Zhou L

    更新日期:2018-07-01 00:00:00

  • Reference Intervals of and Relationships among Essential Trace Elements in Whole Blood of Children Aged 0-14 years.

    abstract:BACKGROUND:Essential trace elements are vital to human health. In this study, our aim was to establish reference intervals of and to evaluate relationships among Ca, Fe, Cu, Mg, and Zn for children. METHODS:We collected blood samples of 3210 children aged 0-14 years from Lu'an, China, and concentrations of the above e...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22043

    authors: Zhai R,Zhang M,Liu J,Guang H,Li B,Chen D,Zhang S

    更新日期:2017-03-01 00:00:00

  • A proposal for standardizing urine collections for bone resorption markers measurement.

    abstract::Diurnal variations in the excretion of bone resorption markers were assessed in order to identify the type of urine collection which provides the most information on bone resorption rate and its relation to measuring bone dynamics in a postmenopausal population. Sixty women, ages 43-67 and without disease or treatment...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/(sici)1098-2825(1998)12:3<145::aid-jcla3>3

    authors: Zaninotto M,Bernardi D,Ujka F,Bonato P,Plebani M

    更新日期:1998-01-01 00:00:00

  • Preliminary study of the relationship between promoter methylation of the ANGPTL2 gene and coronary heart disease.

    abstract:BACKGROUND:Coronary heart disease (CHD) is primarily caused by atherosclerosis of coronary arteries. It is largely an inflammatory disease of the vascular wall. The inflammation is related to DNA methylation. Angiopoietin-like protein 2 (ANGPTL2) has various functions in several chronic inflammatory diseases. Macrophag...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.22702

    authors: Zhou J,Chen L,Yang X,Huang X,Wang Z,Peng P,Lian J

    更新日期:2019-03-01 00:00:00

  • Multivariable index for assessing the activity and predicting all-cause mortality in antineutrophil cytoplasmic antibody-associated vasculitis.

    abstract:BACKGROUND:So far, there has been no tool to estimate activity at diagnosis and predict all-cause mortality in patients with ANCA-associated vasculitis (AAV). Hence, we determined the initial predictors of them in patients with AAV. METHODS:We retrospectively reviewed the medical records of 182 patients with AAV. Seve...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23022

    authors: Kim MK,Choi H,Kim JY,Song JJ,Park YB,Lee SW

    更新日期:2020-01-01 00:00:00

  • An improved microalbumin method (microALB_2) with extended analytical measurement range evaluated on the ADVIA chemistry systems.

    abstract::Quantitative determination of albumin (ALB) in human urine is important to assess kidney functions in a variety of diseases. Recently, Siemens released an improved Microalbumin assay (microALB_2) to measure urinary ALB on the automated, random access ADVIA 1650/1800, ADVIA 2400, and ADVIA 1200 Chemistry Systems. We ev...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20341

    authors: Datta P,Dasgupta A

    更新日期:2009-01-01 00:00:00

  • Racial differences in serum immunoglobulin levels: relationship to cigarette smoking, T-cell subsets, and soluble interleukin-2 receptors.

    abstract::To investigate the influence of race, cigarette smoking, and immunologic parameters on serum immunoglobulins, we analyzed serum IgG, IgA, and IgM levels in 455 healthy adults. The study population ranged in age from 20 to 69 years, including 282 whites and 173 blacks, 181 never-smokers, 93 ex-smokers, and 181 current ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860090107

    authors: Tollerud DJ,Brown LM,Blattner WA,Weiss ST,Maloney EM,Kurman CC,Nelson DL,Hoover RN

    更新日期:1995-01-01 00:00:00

  • Multiplex assay for genetic testing of thrombophilia: a method for routine clinical care.

    abstract::There is an increasing demand for genetic testing of patients with hypercoagulability. We have developed a multiplex-polymerase chain reaction (PCR) technique that incorporates the state-of-the art of genetic testing for thrombosis mutations (Factor V Leiden [FVL] and G20210A F2 and C46T F12). The sequences are detect...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20183

    authors: López M,Giraldo P,Alvarez P,Cornudella R,Pocoví M,Martínez A,Fontcuberta J,Soria JM

    更新日期:2007-01-01 00:00:00

  • Rapid and sensitive headspace gas chromatography-mass spectrometry method for the analysis of ethanol in the whole blood.

    abstract:BACKGROUND:For the forensic aim, a sensitive and specific method using headspace gas chromatography coupled with mass spectrometry (GC/MS) has been developed for the quantitative determination of ethanol in blood using n-propanol as internal standard. GC was performed in isothermal mode with a GC run-time of 5.0 min. ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21698

    authors: Xiao HT,He L,Tong RS,Yu JY,Chen L,Zou J,Li JQ,Bian Y,Zhang Y

    更新日期:2014-09-01 00:00:00

  • Flow cytometric analysis of lymphocyte subpopulations in infants with congenital heart disease.

    abstract::Premortem diagnosis of the DiGeorge syndrome and its partial variants relies on the demonstration of a primary defect in cell-mediated immunity, generally in the setting of an infant with congenital heart disease, hypocalcemia, absence of a thymic shadow, and typical dysmorphic features. Although T-cell enumeration is...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860030105

    authors: Slade HB,Greenwood JH,Beekman RH 3rd,McCoy JP Jr,Hudson JL,Pahwa S,Schwartz SA

    更新日期:1989-01-01 00:00:00

  • Function and regulation annotation of up-regulated long non-coding RNA LINC01234 in gastric cancer.

    abstract:BACKGROUND:Accumulated evidences indicate that long non-coding RNAs (lncRNAs) participate in many biological mechanisms. Moreover, it acts as an essential regulator in various human diseases such as gastric cancer (GC). Nevertheless, the comprehensive regulatory roles and clinical significance of most lncRNAs in GC are...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23210

    authors: Zhu Y,Luo C,Korakkandan AA,Fatma YHA,Tao Y,Yi T,Hu S,Liao Q

    更新日期:2020-05-01 00:00:00

  • Evaluation of conductivity-based osmolality measurement in urine using the Sysmex UF5000.

    abstract:BACKGROUND:Automated flow cytometry-based urine analyzer is increasingly being used to identify and enumerate cells and particles in urine specimens. It measures electrical conductivity which could be transformed to osmolality. Using this machine, all urine specimens could be screened for osmolality without requiring a...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23586

    authors: Yoo DW,Lee SM,Moon SY,Kim IS,Chang CL

    更新日期:2020-09-24 00:00:00

  • Association of SOX11 Polymorphisms in distal 3'UTR with Susceptibility for Schizophrenia.

    abstract:BACKGROUND:Diverse and circumstantial evidence suggests that schizophrenia is a neurodevelopmental disorder. Genes contributing to neurodevelopment may be potential candidates for schizophrenia. The human SOX11 gene is a member of the developmentally essential SOX (Sry-related HMG box) transcription factor gene family ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23306

    authors: Sun CP,Sun D,Luan ZL,Dai X,Bie X,Ming WH,Sun XW,Huo XX,Lu TL,Zhang D

    更新日期:2020-08-01 00:00:00

  • Evaluation of the poly(ADP-ribose) polymerase-1 gene variants in Alzheimer's disease.

    abstract::Amyloid peptide is thought to play a critical role in neuronal death in Alzheimer's disease (AD), most likely through oxidative stress. Free radical-related injury leads to DNA breaks, which subsequently activates the repair enzyme poly(ADP-ribose) polymerase-1 (PARP-1). In this study, the relationship between genetic...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20379

    authors: Liu HP,Lin WY,Wu BT,Liu SH,Wang WF,Tsai CH,Lee CC,Tsai FJ

    更新日期:2010-01-01 00:00:00

  • Problems in determining levels of free digoxin in patients treated with digoxin immune FAb.

    abstract::Determination of free digoxin levels in patients treated with digoxin immune FAb has long been a problematic area in clinical laboratory testing. The older radioimmunoassays resulted in inaccurate and variable results due to the competition of the administered drug with the radioactively labelled forms. The 1995 Physi...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860090612

    authors: Honda SA,Rios CN,Murakami L,Morita T,Scottolini AG,Bhagavan NV

    更新日期:1995-01-01 00:00:00

  • Evaluation of the QMS® Teicoplanin Immunoassay (ThermoFisher Scientific) on Cobas® 8000 System (Roche Diagnostics) and comparison to fluorescence polarization immunoassay for the determination of teicoplanin concentrations in human plasma.

    abstract:BACKGROUND:The performances of the QMS(®) Teicoplanin immunoassay recently developed on Cobas(®) 6000/8000 systems were evaluated and compared to a fluorescence polarization immunoassay (FPIA) [Teicoplanin Innofluor(®) Assay (Thermo Fisher Scientific, Indianapolis, IN)] on FLX analyzer (Abbott Laboratories, Abbott Park...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21567

    authors: Dailly E,Fraissinet F,Deslandes G,Bouquié R,Jolliet P

    更新日期:2013-03-01 00:00:00

  • Study of serum argininosuccinate lyase determination for diagnosis of liver diseases.

    abstract::The objectives of this research were to establish an automatic analysis method for the determination of serum argininosuccinate lyase (ASL) and to investigate the value of serum ASL test in the diagnosis of various liver disorders. According to the chemical reaction catalyzed by ASL, an enzyme-coupled reaction system ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20245

    authors: Feng JF,Chen TM,Wen YA,Wang J,Tu ZG

    更新日期:2008-01-01 00:00:00

  • Detection of Human Epididymis Protein 4 (HE4) in Human Serum Samples Using a Specific Monoclonal Antibody-Based Sandwich Enzyme-Linked Immunosorbent Assay (ELISA).

    abstract:BACKGROUND:The human epididymis protein 4 (HE4) may have high specificity in the detection of malignant diseases, making the development of an immunoassay for HE4 essential. METHODS:In our study, a fusion gene was constructed encoded with the HE4 protein. This protein was then produced in the bacterial cells (Escheric...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21906

    authors: Zhou L,Lv Z,Shao J,Xu Y,Luo X,Zhang Y,Hu Y,Zhang W,Luo S,Fang J,Wang Y,Duan C,Huang R

    更新日期:2016-09-01 00:00:00

  • MicroRNA-146b correlates with decreased acute respiratory distress syndrome risk, reduced disease severity, and lower 28-day mortality in sepsis patients.

    abstract:OBJECTIVE:This study aimed to investigate the predictive value of microRNA-146b (miR-146b) on acute respiratory distress syndrome (ARDS) risk, and the correlation of miR-146b with disease severity and 28-day mortality in sepsis patients. METHODS:A total of 104 sepsis patients and 100 healthy controls (HCs) were consec...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.23510

    authors: Chen W,Liu L,Yang J,Wang Y

    更新日期:2020-08-26 00:00:00

  • N-terminal pro-Brain natriuretic peptide as a useful biomarker for monitoring prognosis in patients with cardiac valve replacement.

    abstract:BACKGROUND:N-terminal pro-Brain natriuretic peptide (NT-proBNP) is an important biomarker to indicate cardiac function, but its significance in cardiac surgery is still unclear. OBJECTIVE:To explore clinical significance of serum NT-proBNP and their dynamic characteristics in patients with cardiac valve replacement (C...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.20435

    authors: Cai B,Wang L,Liu J,Shi Y,Guo Y

    更新日期:2011-01-01 00:00:00

  • Increased serum adiponectin predicts improved coronary flow and clinical outcomes in patients with ST-segment elevation myocardial infarction treated by primary percutaneous coronary intervention.

    abstract:BACKGROUND:Previous studies suggested that adiponectin (APN) could ameliorate ischemia/reperfusion injury and endothelial dysfunction in patients with acute myocardial infarction. However, the relationship between serum APN level and coronary flow after primary percutaneous coronary intervention (PPCI) in patients with...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 临床试验,杂志文章

    doi:10.1002/jcla.22864

    authors: Liang S,Li H,Shen X,Liu R

    更新日期:2019-06-01 00:00:00

  • Comparative evaluation of a new chemiluminiscent assay and an ELISA for the detection of IgM against measles.

    abstract:BACKGROUND:The primary test for the laboratory confirmation of measles is immunoglobulin M (IgM) serology. It is therefore important to evaluate new commercial measles IgM immunoassays to ensure high-quality measles diagnostic testing. The purpose of this study was to evaluate the diagnostic performance of LIAISON IgM ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.21630

    authors: Sampedro A,Rodríguez-Granger J,Gómez C,Lara A,Gutierrez J,Otero A

    更新日期:2013-11-01 00:00:00

  • Measurement of serum IgA and C3 may predict the diagnosis of patients with IgA nephropathy prior to renal biopsy.

    abstract::The levels of serum IgA and C3 in patients with IgA nephropathy were determined using international standard serum (IFCC/CRM470) in a multicenter trial in Japan. The ratio of serum IgA to C3 (serum IgA/C3 ratio) without any information from renal biopsy was used for the diagnosis of IgA nephropathy. Three hundred and ...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:

    authors: Tomino Y,Suzuki S,Imai H,Saito T,Kawamura T,Yorioka N,Harada T,Yasumoto Y,Kida H,Kobayashi Y,Endoh M,Sato H,Saito K

    更新日期:2000-01-01 00:00:00

  • Rhabdomyosarcoma cell line can be used for the isolation of soluble acetylcholine receptor and for assaying blocking and modulating autoantibodies.

    abstract::We found that the Rhabdomyosarcoma (RD) cell line expresses human acetylcholine receptor (AChR) based on the following evidences: 1. Soluble AChR can be isolated from RD cells following the isolation procedure for AChR from human muscle; 2. Intact RD cells bind to alpha-bungarotoxin (alpha Butx) in a time-dependent an...

    journal_title:Journal of clinical laboratory analysis

    pub_type: 杂志文章

    doi:10.1002/jcla.1860070104

    authors: Wu JT,Astill M,Lloyd C,Salmon VC

    更新日期:1993-01-01 00:00:00