Abstract:
:A procedure utilizing co-chromatography and complementary antiserum comparisons was employed to assess the specificity of a cortisol radioimmunoassay for use in the chronically catheterized fetal sheep preparation. Complementary antiserum comparisons is a technique by which two different cortisol antisera, prepared from conjugates attached at opposite ends of the cortisol molecule, were used to determine cortisol concentrations in the same ovine fetal plasma specimens. Results were not significantly different between the two groups, each measured by a different antiserum. This procedure may be used to assess assay specificity in any species in which steroid radioimmunoassays are being adapted.
journal_name
Steroidsjournal_title
Steroidsauthors
Magyar DM,Buster JE,Elsner CW,Nathanielsz PW,Oliveros R,Abraham GEdoi
10.1016/s0039-128x(79)80005-7subject
Has Abstractpub_date
1979-01-01 00:00:00pages
45-53issue
1eissn
0039-128Xissn
1878-5867pii
S0039-128X(79)80005-7journal_volume
33pub_type
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