Abstract:
:Cotyledons of dark-grown watermelon seedlings contain during the first period of germination, 4 and later 5 MDH isoenzymes. Whereas isoenzymes I, II, and IV belong to the cytosol, isoenzyme III is confined to the mitochondria (mMDH) and isoenzyme V to the glyoxysomes (gMDH). The organelle-bound MDHs were purified to homogeneity and characterized. They are remarkably similar with respect to their kinetic properties, but differ widely in other characteristics, e.g. MW, IEP, thermostability, and serological properties. Both isoenzymes are synthesized de novo during germination by cytoplasmic ribosomes. In a reticulocyte cell-free system programmed by watermelon poly (A+) RNA, gMDH and mMDH are synthesized as larger precursors as compared to the authentic polypeptide chains, with an extra sequence of ca. 8.0 kilodaltons in the case of gMDH and ca. 3.3 kilodaltons with mMDH. The processing of the gMDH precursor by an enriched organelle fraction in not complete. If the post-translational incorporation of the in vitro product into the organelles is followed by proteinase treatment that completely hydrolyzes noncompartmentalized gMDH, the authentic product is obtained. A model for the transport mechanism is given.
journal_name
Ann N Y Acad Scijournal_title
Annals of the New York Academy of Sciencesauthors
Hock B,Gietl Cdoi
10.1111/j.1749-6632.1982.tb21427.xsubject
Has Abstractpub_date
1982-01-01 00:00:00pages
350-76eissn
0077-8923issn
1749-6632journal_volume
386pub_type
杂志文章abstract::Myasthenia gravis (MG) in individuals over 40 years of age comprises three groups: early-onset MG with prolonged disease duration, late-onset MG with thymoma, and late-onset of nonthymomatous MG. The clinical features do not differ between the three groups, except that early-onset patients with prolonged disease durat...
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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journal_title:Annals of the New York Academy of Sciences
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