Purification and biochemical characterization of an extracellular endoglucanase from the necrotrophic oomycete, Pythium myriotylum Dreschler.

abstract：:An outbreak of serious mortality among the cultured cobia Rachycentron canadum (weighing 3 kg) characterized by the presence of whitish granulomatous deposits on the kidney, liver and spleen occurred in July of 2000 in Taiwan. A non-motile strain CP1 was isolated from kidney and/or liver on tryptic soy agar and/or bra...

journal_title：Journal of basic microbiology

authors： Liu PC,Lin JY,Lee KK

更新日期：2003-01-01 00:00:00

abstract：:Sixteen Bacillus thuringiensis (Bt) strains were screened for their anti-insect, antibacterial and antifungal determinants by phenotypic tests and PCR targeting major insecticidal proteins and complements, chitinases, lactonases, beta-1,3-glucanases and zwittermicinA. Six strains had genes of at least two major insect...

journal_title：Journal of basic microbiology

authors： Raddadi N,Belaouis A,Tamagnini I,Hansen BM,Hendriksen NB,Boudabous A,Cherif A,Daffonchio D

更新日期：2009-06-01 00:00:00

abstract：:A Sau3AI digest of total DNA from Saccharomycopsis fibuligera was cloned with the yeast-integrating plasmid YEp24 delta EcoRI and the capacity for autonomous replication (ARS) was assayed in yeast. From eighty clones, five mitoticaly unstable yeast transformants were picked up, recombinant plasmids from these clones w...

journal_title：Journal of basic microbiology

authors： Jurský F,Hostinová E

更新日期：1990-01-01 00:00:00

abstract：:Five-hundreds and eighty bacterial strains, isolated from various Antarctic marine sources and locations, were screened for antimicrobial activity against terrestrial microorganisms. Twenty-two Antarctic isolates (3.8%), mainly retrieved from the water column at Terra Nova Bay (Ross Sea), expressed antagonistic activi...

journal_title：Journal of basic microbiology

authors： Lo Giudice A,Bruni V,Michaud L

更新日期：2007-12-01 00:00:00

abstract：:To discuss the microbial structure that develops during the course of compost burials of polyvinyl alcohol (PVA)-based material and analyze the effect of the degree of PVA alcoholysis on the PVA-degrading ability of each strain. Two bacterial strains exhibiting a high level of PVA-degrading ability and two mixed cultu...

journal_title：Journal of basic microbiology

authors： Liu Y,Deng Y,Chen P,Duan M,Lin X,Zhang Y

更新日期：2019-04-01 00:00:00

abstract：:A culture was isolated from an industrial mercuric salt-contaminated soil, which could tolerate Cd, Co, Zn, Cr, and Hg up to 190, 525, 350, 935, and 370 μM, respectively. The isolate was identified as Lysinibacillus sphaericus by 16S rRNA gene sequencing. It bioaccumulated Cd, Co, and Zn, and reductively detoxified Cr...

journal_title：Journal of basic microbiology

authors： Bafana A,Chakrabarti T,Krishnamurthi K

更新日期：2015-03-01 00:00:00

abstract：:A study was conducted to examine the prevalence of brucellosis (in animal farms) in the vicinity of Islamabad and Rawalpindi. A total of 170 milk samples were collected randomly from several farmhouses. The collected milk samples were initially screened by a Brucella selective medium. The bacterial isolates grown on t...

journal_title：Journal of basic microbiology

authors： Khan S,Akhtar MU,Khan S,Javed F,Khan AA

更新日期：2020-03-01 00:00:00

abstract：:This work reports the production of a novel serine protease enzyme (P. dig-protease) from the fungus Penicillium digitatum. The protease was purified from the culture supernatant to homogeneity using ammonium sulfate precipitation, Sephadex G-150 gel filtration and carboxymethyl-sepharose ion exchange chromatography w...

journal_title：Journal of basic microbiology

authors： Aissaoui N,Abidi F,Mahat S,Marzouki MN

更新日期：2014-07-01 00:00:00

abstract：:Supercoiling of bacterial DNA is regulated by topoisomerases and influences most of the metabolic processes involving DNA. The present review is devoted to a brief outline of the supercoiled state of DNA in bacteria and to all microbial topoisomerases hitherto described. Recent studies on topoisomerases of archaebacte...

journal_title：Journal of basic microbiology

authors： Zimmer C,Störl K,Störl J

更新日期：1990-01-01 00:00:00

abstract：:Spontaneously occurring thallium-resistant (Tl+ -R) mutant of diazotrophic, heterocystous cyanobacterium Nostoc muscorum is described in which Tl+ -R phenotype is the cause of defective salinity-inducible K+ uptake activity and proline uptake activity. The results indicate that mutant strain is more sensitive to salin...

journal_title：Journal of basic microbiology

authors： Bhargava S

更新日期：2005-01-01 00:00:00

abstract：:Microorganisms such as bacteria and molds produce an enormous variety of volatile metabolites. To determine whether typical microbial volatile metabolites can be used as indicator compounds for the detection of hidden mold in indoor environments, we examined 14 typical indoor fungal strains for their growth rates and ...

journal_title：Journal of basic microbiology

authors： Schuchardt S,Kruse H

更新日期：2009-08-01 00:00:00

abstract：:A total of 216 killer yeasts Saccharomyces cerevisiae, isolated from wine, were evaluated in controlling Colletotrichum gloeosporioides, a pre-harvest anthracnose agent of grape. Three of these yeast isolates were tested positive for antagonizing C. gloeosporioides and were further evaluated for their mechanisms as bi...

journal_title：Journal of basic microbiology

authors： Liu Z,Du S,Ren Y,Liu Y

更新日期：2018-01-01 00:00:00

abstract：:Fifteen actinomycete strains were evaluated for their potential use in removal of polycyclic aromatic hydrocarbons (PAH). Their capability to degrade of naphthalene, phenanthrene, and pyrene was tested in minimal medium (MM) and MM with glucose as another substrate. Degradation of naphthalene in MM was observed in all...

journal_title：Journal of basic microbiology

authors： Bourguignon N,Isaac P,Alvarez H,Amoroso MJ,Ferrero MA

更新日期：2014-12-01 00:00:00

abstract：:A nourseothricin-producing Streptomyces noursei strain was continuously cultivated in a chemostat equipped with a stirrer for mechanical fractionation of the mycelium. Different cultivation conditions allowed the selection of six types of differentiation mutants after the culture had reached a population genetically s...

journal_title：Journal of basic microbiology

authors： Noack D

更新日期：1986-01-01 00:00:00

abstract：:The objective of this study was to identify the most promising nutritional mode of growth for enhanced biomass and lipid productivity in a set of twenty microalgal strains, grown under photoautotrophic and mixotrophic/heterotrophic conditions using 2% glucose as carbon source. These included four cyanobacterial strain...

journal_title：Journal of basic microbiology

authors： Ratha SK,Babu S,Renuka N,Prasanna R,Prasad RB,Saxena AK

更新日期：2013-05-01 00:00:00

abstract：:A set of 50 randomly chosen fungal strains belonging to different basidiomycete species was tested for H2O2 and ligninolytic enzyme production and for decolorization of synthetic dyes Orange G and Remazol Brilliant Blue R. The decolorization capacity of individual strains was influenced by the level of H2O2 and laccas...

journal_title：Journal of basic microbiology

authors： Eichlerová I,Homolka L,Lisá L,Nerud F

更新日期：2006-01-01 00:00:00

abstract：:beta -Glucosidase is a highly desired glycosidase, especially for hydrolysis of glycoconjugated precursors in musts and wines for the release of active aromatic compounds. A Shewanella sp. G5 strain was isolated from the intestinal content of benthonic organism (Munida subrrugosa) from different coastal areas of the B...

journal_title：Journal of basic microbiology

authors： Cristóbal HA,Breccia JD,Abate CM

更新日期：2008-02-01 00:00:00

abstract：:A comparative electron microscopic analysis of weakly beta-haemolytic spirochaetes related to human and animal intestinal spirochaetosis was done in order to search for the presence of inducible bacteriophages associated with these spirochaetes. Bacteriophages were detected at the electron microscope after experimenta...

journal_title：Journal of basic microbiology

authors： Calderaro A,Dettori G,Collini L,Ragni P,Grillo R,Cattani P,Fadda G,Chezzi C

更新日期：1998-01-01 00:00:00

abstract：:Biofilms are means of protection to bacteria against antibiotics and antibodies. Catheters and others tube devices used by patients are prone to accumulation of thick layers of biofilms as hiding place for etiologic agents, resulting in substantial morbidity and mortality. Methicillin-resistant Staphylococcus aureus (...

journal_title：Journal of basic microbiology

authors： Mirani ZA,Jamil N

更新日期：2011-04-01 00:00:00

abstract：:Thermus strains are regarded as models to investigate the mechanism of thermostability of thermophiles, and phages from Thermus are particularly interesting because of their way to regulate gene expression. In this research, a Thermus bacteriophage named TSP4 (Thermus Siphoviridae phage) was isolated from Tengchong ho...

journal_title：Journal of basic microbiology

authors： Lin L,Hong W,Ji X,Han J,Huang L,Wei Y

更新日期：2010-10-01 00:00:00

abstract：:To understand the physiology of non-differentiating bacteria exposed to nutrient deprivation and stress, various approaches have been employed in combination with detailed analysis of protein synthesis pattern. In this study, separation of proteins from clarified cell extracts of Pseudomonas aeruginosa PAO1 grown unde...

journal_title：Journal of basic microbiology

authors： Madhusudhan KT,McLaughlin R,Komori N,Matsumoto H

更新日期：2003-01-01 00:00:00

abstract：:Helicobacter pylori exhibit morphology convertion in a spiral or coccoid form. This study aims to reveal the impact of coccoid H. pylori on the proliferation and apoptosis of gastric epithelial cells. The cagA and vacA genes of H. pylori were detected by semi-quantitative RT-PCR. Proliferation and apoptosis were analy...

journal_title：Journal of basic microbiology

authors： Li N,Han L,Chen J,Lin X,Chen H,She F

更新日期：2013-02-01 00:00:00

abstract：:Screening new organisms for antibacterial activity and searching for new antibacterial drugs is important due to the constant generation of new antibiotic-resistant strains of pathogenic bacteria. An E. coli broth microdilution test was used to evaluate the results of the Vibrio fischeri bioluminescence test in five o...

journal_title：Journal of basic microbiology

authors： Janes D,Umek A,Kreft S

更新日期：2006-01-01 00:00:00

abstract：:Swainsonine is a polyhydroxylated indolizidine alkaloid having anticancer, antimetastatic, antiproliferative and immunomodulatory activities and also potential therapeutic applications against AIDS. In the present study, ten isolates of M. anisopliae were screened and enzyme assayed for the production of swainsonine i...

journal_title：Journal of basic microbiology

authors： Singh D,Kaur G

更新日期：2012-10-01 00:00:00

abstract：:An extracellular lipase isolated from Bacillus megaterium AKG-1 had an optimum activity at 55 degrees C/pH 7.0. It retained 100% activity at 50 degrees C for 30 min with a half life of 30 min at 70 degrees C. A 20-70% increase in lipase activity was observed in presence of acetone (20% v/v), DMSO (20% v/v) and isoprop...

journal_title：Journal of basic microbiology

authors： Sekhon A,Dahiya N,Tiwari RP,Hoondal GS

更新日期：2005-01-01 00:00:00

abstract：:Spodoptera littoralis, one of the major pests of many important crop plants, is more susceptible to Bacillus thuringiensis aizawai delta-endotoxins than to those of Bacillus thuringiensis kurstaki. Within the framework of the development of efficient bioinsecticides and the prevention against insect resistance, we tes...

journal_title：Journal of basic microbiology

authors： Benfarhat-Touzri D,Ben Amira A,Ben khedher S,Givaudan A,Jaoua S,Tounsi S

更新日期：2014-11-01 00:00:00

abstract：:Two shuttle vectors named pRC52 (10.7 Kb) and pRK52 (12.2 Kb) carrying chloramphenicol (Cm) and chloramphenicol plus kanamycin (Km) resistance genes, respectively, were constructed by fusion of a cryptic plasmid pBL13869 (replicon pBL1, 5.8 Kb) from Brevibacterium lactofermentum ATCC13869 with pBR328 E. coli plasmid. ...

journal_title：Journal of basic microbiology

authors： Duran R

更新日期：1998-01-01 00:00:00

abstract：:Quorum sensing is a cell-cell signaling mechanism based on cell density and that involves the production of hormone-like molecules called autoinducers (AI). One of the most studied AIs has been termed AI-2, and its biosynthesis requires the enzyme encoded by luxS. We have previously described for the first time that B...

journal_title：Journal of basic microbiology

authors： Peixoto RJ,Miranda KR,Ferreira EO,de Paula GR,Rocha ER,Lobo LA,Domingues RM

更新日期：2014-07-01 00:00:00

abstract：:A comparative study of the wild type A. nidulans and a mutant strain aco-T69 (lacking conidia and cleistothecia) revealed the better production of beta-1,4-glucosidase in the former. The relative distribution of the enzyme levels in various morphological structures viz. somatic hyphae, spores and cleistothecia also sh...

journal_title：Journal of basic microbiology

authors： Sharma S,Bagga PS,Sandhu DK

更新日期：1990-01-01 00:00:00

abstract：:Leaf-cutting ants modify the properties of the soil adjacent to their nests. Here, we examined whether such an ant-altered environment impacts the belowground fungal communities. Fungal diversity and community structure of soil from the fungus garden chambers of Atta sexdens rubropilosa and Atta bisphaerica, two wides...

journal_title：Journal of basic microbiology

authors： Rodrigues A,Passarini MR,Ferro M,Nagamoto NS,Forti LC,Bacci M Jr,Sette LD,Pagnocca FC

更新日期：2014-11-01 00:00:00