New shuttle vectors for Rhodococcus sp. R312 (formerly Brevibacterium sp. R312), a nitrile hydratase producing strain.

Abstract:

:Two shuttle vectors named pRC52 (10.7 Kb) and pRK52 (12.2 Kb) carrying chloramphenicol (Cm) and chloramphenicol plus kanamycin (Km) resistance genes, respectively, were constructed by fusion of a cryptic plasmid pBL13869 (replicon pBL1, 5.8 Kb) from Brevibacterium lactofermentum ATCC13869 with pBR328 E. coli plasmid. Transformation of Rhodococcus sp. R312 (formerly Brevibacterium sp. R312) protoplasts was realised with an efficiency of 28 transformants per micrograms of DNA.

journal_name

J Basic Microbiol

authors

Duran R

subject

Has Abstract

pub_date

1998-01-01 00:00:00

pages

101-6

issue

2

eissn

0233-111X

issn

1521-4028

pii

10.1002/(SICI)1521-4028(199805)38:2<101::AID-JOBM1

journal_volume

38

pub_type

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