Qualitative and quantitative changes in beta-1,4-glucosidase accompanying growth of Aspergillus nidulans.

Abstract:

:A comparative study of the wild type A. nidulans and a mutant strain aco-T69 (lacking conidia and cleistothecia) revealed the better production of beta-1,4-glucosidase in the former. The relative distribution of the enzyme levels in various morphological structures viz. somatic hyphae, spores and cleistothecia also showed a variation. Highest specific activity was found in the cleistothecial extracts. The electrophoretic analysis of the wild type strain demonstrated the presence of three isoenzymes of beta-1,4-glucosidase i.e. beta-GLU I, beta-GLU II and beta-GLU III, the number of which varies from one to three during the growth of the organism. The parallel study of the mutant strain showed complete absence of beta-GLU II. All three forms were present in the somatic hyphae and cleistothecial extracts while spore extracts depicted the absence of beta-GLU III in the wild type.

journal_name

J Basic Microbiol

authors

Sharma S,Bagga PS,Sandhu DK

doi

10.1002/jobm.3620300513

subject

Has Abstract

pub_date

1990-01-01 00:00:00

pages

363-70

issue

5

eissn

0233-111X

issn

1521-4028

journal_volume

30

pub_type

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