Abstract:
:Studies were carried out to isolate chlorpyrifos degrading Bacillus thuringiensis (Bt) strains from chlorpyrifos-contaminated samples. Six Bt strains (isolation rate 2.7%) were isolated by modified sodium acetate antibiotic heat treatment, and one novel strain (BRC-HZM2) was selected for further analysis. Phenotype and phylogeny analysis of this strain was conducted on the basis of biochemical reactions, antibiotic sensitivity, 16s rRNA genes, plasmid profile, insecticidal crystal protein profiles, and PCR-RFLP for cry and cyt genes. The degradation rate of chlorpyrifos in liquid culture was estimated during 48 h of incubation for the isolate BRC-HZM2. More than 50% of the initial chlorpyrifos concentration degraded within 12 h, 88.9% after 48 h. These results highlight the potential of the Bt strain for biological control and the bioremediation of environments contaminated with chlorpyrifos.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Wu S,Peng Y,Huang Z,Huang Z,Xu L,Ivan G,Guan X,Zhang L,Zou Sdoi
10.1002/jobm.201300501subject
Has Abstractpub_date
2015-03-01 00:00:00pages
389-97issue
3eissn
0233-111Xissn
1521-4028journal_volume
55pub_type
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