Polyphenol oxidase from Pectobacterium atrosepticum: identification and cloning of gene and characteristics of the enzyme.

abstract：:A 2-haloacid dehalogenase-producing bacterium, designated DEH99, was isolated from the marine sponge Hymeniacidon perlevis using a modified enrichment medium and a pH indicator method. DEH99 could degrade only half of the racemic mixture 2-chloropropionic acid (2-CPA) in the medium. The dehalogenase of DEH99 was furth...

journal_title：Journal of basic microbiology

authors： Huang J,Xin Y,Zhang W

更新日期：2011-06-01 00:00:00

abstract：:Microorganisms such as bacteria and molds produce an enormous variety of volatile metabolites. To determine whether typical microbial volatile metabolites can be used as indicator compounds for the detection of hidden mold in indoor environments, we examined 14 typical indoor fungal strains for their growth rates and ...

journal_title：Journal of basic microbiology

authors： Schuchardt S,Kruse H

更新日期：2009-08-01 00:00:00

abstract：:Membrane-attached nucleoids were isolated from E. coli and separated from proteins by 2 M NaCl. Disintegration of such nucleoids by ultrasound and subsequent centrifugation resulted in the formation of two fractions: a sediment (fraction I) and a supernatant (fraction II). The protein:DNA ratio of fraction I was equal...

journal_title：Journal of basic microbiology

authors： Gaziev AI,Fomenko LA,Zakrzhevskaya DT,Petrov SI

更新日期：1986-01-01 00:00:00

abstract：:Cassava wastes--the peel and the root fibre were taken through various pretreatment procedures before being subjected to solid state fermentation with Trichoderma harzianum. Most of the pretreatment processes increased the cellulose and hemicellulose content of the cassava peel and fibre by as high as 155% while sulfu...

journal_title：Journal of basic microbiology

authors： Onilude AA

更新日期：1996-01-01 00:00:00

abstract：:L-form strains of Proteus mirabilis and Escherichia coli lacking the cell wall represent an alternative prokaryotic cell system for the production of recombinant proteins (KLESSEN et al. 1988, LAPLACE et al. 1988a, 1989b). We could demonstrate that they are also able to synthesize the enzyme penicillin G acylase (PAC)...

journal_title：Journal of basic microbiology

authors： Gumpert J,Cron H,Plapp R,Niersbach H,Hoischen C

更新日期：1996-01-01 00:00:00

abstract：:The gene mak1FN coding for maltokinase from Actinoplanes missouriensis is located in a cluster similar to glycogen metabolism clusters identified in Streptomyces coelicolor. Sequence comparisons demonstrate that mak1-related genes coding for homologous proteins are present in many bacterial genomes including taxonomic...

journal_title：Journal of basic microbiology

authors： Jarling M,Cauvet T,Grundmeier M,Kuhnert K,Pape H

更新日期：2004-01-01 00:00:00

abstract：:Tricholoma matsutake (S. Ito et Imai) is an ectomycorrhizal basidiomycete associated with Pinaceae and Fagaceae trees in the Northern Hemisphere. It is still unknown whether the symbiotic relationship with this ectomycorrhiza could affect the host plant's endophytic microbial community. In this study, we used high thr...

journal_title：Journal of basic microbiology

authors： Li Q,Xiong C,Li X,Jin X,Huang W

更新日期：2018-03-01 00:00:00

abstract：:The high-level expression of the xylanase GH11 gene from Aspergillus niger IA-001 called xynB was successfully completed in Pichia pastoris. The xynB gene encoding a mature xylanase of 225 amino acid was subcloned into the pPICZαA vector and was transformed into P. pastoris X-33 under the control of the alcohol oxidas...

journal_title：Journal of basic microbiology

authors： Fang W,Gao H,Cao Y,Shan A

更新日期：2014-07-01 00:00:00

abstract：:Crude glucoamylase preparation from Rhizoctonia solani was used to saccharify raw and cooked starch. Various concentrations of potato starch and wheat flour from 10-40%, w/v were used for mashing but 30% was found to be the optimal and economical. The saccharified mash yielded 5.89%, v/v ethanol in a simultaneous sacc...

journal_title：Journal of basic microbiology

authors： Singh D,Dahiya JS,Nigam P

更新日期：1995-01-01 00:00:00

abstract：:A soluble, NADP+-dependent alcohol dehydrogenase (ADH) with a pH optimum at 8.7 was found in A. calcoaceticus EB 104 after growth on different carbon sources. n-Alkanols with short and medium chain length were employed as test substrates. The Km values decreased with increasing chain length. The Vmax values remained n...

journal_title：Journal of basic microbiology

authors： Jirausch M,Asperger O,Kleber HP

更新日期：1986-01-01 00:00:00

abstract：:Industrial effluents contaminated sites may serve as repositories of ecologically adapted efficient pyrene degrading bacteria. In the present study, six bacterial isolates from industrial effluents were purified using serial enrichment technique and their pyrene degrading potential on pyrene supplemented mineral salt ...

journal_title：Journal of basic microbiology

authors： Meena SS,Sharma RS,Gupta P,Karmakar S,Aggarwal KK

更新日期：2016-04-01 00:00:00

abstract：:The impact of increasing Cd2+ exposure on the quality and quantity of siderophores produced by a plant growth promoting Pseudomonas fulva strain was tested to gain insight into the degree of change. P. fulva was cultured in the increasing concentrations of Cd2+ (0, 0.5, 1.0, 2.0 mM). The secreted siderophores were sep...

journal_title：Journal of basic microbiology

authors： Thiem D,Złoch M,Gadzała-Kopciuch R,Szymańska S,Baum C,Hrynkiewicz K

更新日期：2018-07-01 00:00:00

abstract：:A comparative study of the wild type A. nidulans and a mutant strain aco-T69 (lacking conidia and cleistothecia) revealed the better production of beta-1,4-glucosidase in the former. The relative distribution of the enzyme levels in various morphological structures viz. somatic hyphae, spores and cleistothecia also sh...

journal_title：Journal of basic microbiology

authors： Sharma S,Bagga PS,Sandhu DK

更新日期：1990-01-01 00:00:00

abstract：:Leaf-cutting ants modify the properties of the soil adjacent to their nests. Here, we examined whether such an ant-altered environment impacts the belowground fungal communities. Fungal diversity and community structure of soil from the fungus garden chambers of Atta sexdens rubropilosa and Atta bisphaerica, two wides...

journal_title：Journal of basic microbiology

authors： Rodrigues A,Passarini MR,Ferro M,Nagamoto NS,Forti LC,Bacci M Jr,Sette LD,Pagnocca FC

更新日期：2014-11-01 00:00:00

abstract：:The steroid 1-dehydrogenase of Rhodococcus erythropolis IMET 7030, an active steroid-transforming strain, was localized by immunogold labelling both in cells induced with 17-alpha-methyl-testosterone and in noninduced cells. The labelling intensity was much higher in induced cells than in noninduced cells, indicating ...

journal_title：Journal of basic microbiology

authors： Wagner B,Atrat PG,Clark-Curtiss JE,Wagner M

更新日期：1992-01-01 00:00:00

abstract：:An extracellular endoglucanase (EG) that catalyzes the hydrolysis of carboxy-methyl cellulose (CMC) as substrate was purified to homogeneity from the soft-rot causing oomycete P. myriotylum with maximum EG production observed in presence of 1% (w/v) sucrose. The enzyme designated PmEG was observed to be monomeric with...

journal_title：Journal of basic microbiology

authors： Geethu C,Nair RA

更新日期：2014-12-01 00:00:00

abstract：:Acinetobacter spp. are ubiquitous obligate aerobic bacteria which occur mostly as commensals on the skin, and in soil, water and plants' rhizosphere. Though the species in this genus have been implicated as aetiologies in some nosocomial infections, their versatility covers biodegradation or dissolution leading to bio...

journal_title：Journal of basic microbiology

authors： Adegoke AA,Mvuyo T,Okoh AI

更新日期：2012-12-01 00:00:00

abstract：:Hyoscyamus muticus L. (Egyptian henbane) is one of the desert medicinal plants of family Solanaceae. The plant produces pharmaceutically important compounds (tropane alkaloids) as secondary metabolites. In the present study, we describe mycoflora of H. muticus grown in four different locations in Egyptian southern des...

journal_title：Journal of basic microbiology

authors： El-Zayat SA,Nassar MS,El-Hissy FT,Abdel-Motaal FF,Ito S

更新日期：2008-04-01 00:00:00

abstract：:Screening new organisms for antibacterial activity and searching for new antibacterial drugs is important due to the constant generation of new antibiotic-resistant strains of pathogenic bacteria. An E. coli broth microdilution test was used to evaluate the results of the Vibrio fischeri bioluminescence test in five o...

journal_title：Journal of basic microbiology

authors： Janes D,Umek A,Kreft S

更新日期：2006-01-01 00:00:00

abstract：:Using arbitrary primers for a PCR reaction on cDNA from different mating interactions, genes differentially expressed in a mating-type dependent manner have been identified. The cDNAs were prepared from mRNA extracted from liquid grown cultures of the fungus in order to prevent further steps in development and increas...

journal_title：Journal of basic microbiology

authors： Schubert D,Lengeler KB,Kothe E

更新日期：2000-01-01 00:00:00

abstract：:An Indian indigenous, Loktak Lake soil isolate Streptomyces violascens MT7 was assessed for its biocontrol potential both in vitro and in vivo against toxigenic fruit-rotting fungi. Strain MT7 exhibited broad-spectrum antifungal activity against various pathogenic postharvest fungi of citrus and papaya. In shake-flask...

journal_title：Journal of basic microbiology

authors： Choudhary B,Nagpure A,Gupta RK

更新日期：2015-12-01 00:00:00

abstract：:A gene encoding an organic solvent-stable protease was amplified from Pseudomonas aeruginosa strain K by polymerase chain reaction using consensus primers based on multiple sequence alignment of alkaline and metalloprotease genes from Pseudomonas species. The gene, which consisted of 1440 bp nucleotides and deduced 47...

journal_title：Journal of basic microbiology

authors： Rahman RN,Geok LP,Wong CF,Basri M,Salleh AB

更新日期：2010-04-01 00:00:00

abstract：:Many ecosystems are currently co-contaminated with heavy metals such as cadmium (Cd(2+) ) and pesticides such as chlorpyrifos (CP) and γ-hexachlorocyclohexane (γ-HCH). A feasible approach to remediate the combined pollution of heavy metals and pesticides is the use of γ-HCH degrading bacteria endowed with CP hydrolysi...

journal_title：Journal of basic microbiology

authors： Yang C,Yu H,Jiang H,Qiao C,Liu R

更新日期：2016-07-01 00:00:00

abstract：:An alkaline serine protease produced by Bacillus megaterium RRM2 isolated from the red alga, Kappaphycus alvarezii (Doty) Doty ex Silva was studied for the first time and the same analyzed for the production of protease in the present study. Identification of the bacterium was done on the basis of both biochemical ana...

journal_title：Journal of basic microbiology

authors： Rajkumar R,Jayappriyan KR,Rengasamy R

更新日期：2011-12-01 00:00:00

abstract：:The present study describes the biosynthesis of proteases by a locally isolated mould culture of Rhizopus oligosporus IHS13 in a low-cost medium by solid-state fermentation technique. The fermentation was carried out in a low-cost medium such as sunflower meal, wheat bran and rice bran. Sunflower meal and wheat bran i...

journal_title：Journal of basic microbiology

authors： Haq IU,Mukhtar H

更新日期：2004-01-01 00:00:00

abstract：:The yeast Cryptococcus sp. UFMG-Y28 can utilize benzonitrile as a nitrogen and possible additional carbon source. The kinetics of growth on Yeast Carbon Base (YCB) added of benzonitrile as sole nitrogen source showed that benzonitrile was metabolized to benzoic acid and ammonia. Liquid chromatography analysis indicate...

journal_title：Journal of basic microbiology

authors： Rezende RP,Dias JC,Ferraz V,Linardi VR

更新日期：2000-01-01 00:00:00

abstract：:The analysis of RubisCO genes is a highly useful instrument to explore the diversity of chemoautotrophic bacteria using the Calvin-Benson-Bassham cycle for CO2 fixation. However, because of the wide taxonomic distribution of phylogenetically related RubisCO forms, environmental studies targeting chemoautotrophs are ha...

journal_title：Journal of basic microbiology

authors： Alfreider A,Bogensperger T

更新日期：2018-08-01 00:00:00

abstract：:In the past years, research on the flagellate unicellular alga Chlamydomonas reinhardtii has entered a new era based on the availability of its complete genome. Since this green alga can be grown relatively easy in a short time-range, sufficient biological material is available to efficiently establish biochemical pur...

journal_title：Journal of basic microbiology

authors： Wagner V,Boesger J,Mittag M

更新日期：2009-02-01 00:00:00

abstract：:Rock phosphate effect on English mint (Mentha piperita L.) grown on steamed perlite:vermiculite (1:1, v:v) substrate, with and without rock phosphate, was evaluated in greenhouse experiments. Five treatments were carried out by inoculation with an arbuscular mycorrhizal fungus Glomus mosseae and a phosphorus solubiliz...

journal_title：Journal of basic microbiology

authors： Cabello M,Irrazabal G,Bucsinszky AM,Saparrat M,Schalamuk S

更新日期：2005-01-01 00:00:00

abstract：:Lipases play an important role in the infection process of entomopathogenic fungi by hydrolyzing the ester bonds of lipoproteins, fats and waxes present on the insect surface and in the body. Here we report the purification and characterization of an extracellular lipase from Isaria fumosorosea. The enzyme was purifie...

journal_title：Journal of basic microbiology

authors： Ali S,Ren S,Huang Z

更新日期：2014-11-01 00:00:00