Localization of the steroid 1-dehydrogenase in Rhodococcus erythropolis IMET 7030 by immunoelectron microscopy.

Abstract:

:The steroid 1-dehydrogenase of Rhodococcus erythropolis IMET 7030, an active steroid-transforming strain, was localized by immunogold labelling both in cells induced with 17-alpha-methyl-testosterone and in noninduced cells. The labelling intensity was much higher in induced cells than in noninduced cells, indicating increased enzyme production in the case of induction. Using the postembedding procedure, the main portion of the enzyme was found in the peripheral region of the cytoplasm. A considerable amount was bound in clusters to the inner side of the cytoplasmic membrane. The enzyme was also detected in channels connecting the cytoplasm with the cell surface. By means of the preembedding labelling, a few gold clusters could be detected on the cell surface. The significance of this observation was discussed.

journal_name

J Basic Microbiol

authors

Wagner B,Atrat PG,Clark-Curtiss JE,Wagner M

doi

10.1002/jobm.3620320119

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

65-71

issue

1

eissn

0233-111X

issn

1521-4028

journal_volume

32

pub_type

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