Effect of temperature and medium composition on mycelial growth of Streptomyces tendae in submerged culture.

abstract：:This work reports the production of a novel serine protease enzyme (P. dig-protease) from the fungus Penicillium digitatum. The protease was purified from the culture supernatant to homogeneity using ammonium sulfate precipitation, Sephadex G-150 gel filtration and carboxymethyl-sepharose ion exchange chromatography w...

journal_title：Journal of basic microbiology

authors： Aissaoui N,Abidi F,Mahat S,Marzouki MN

更新日期：2014-07-01 00:00:00

abstract：:The gene mak1FN coding for maltokinase from Actinoplanes missouriensis is located in a cluster similar to glycogen metabolism clusters identified in Streptomyces coelicolor. Sequence comparisons demonstrate that mak1-related genes coding for homologous proteins are present in many bacterial genomes including taxonomic...

journal_title：Journal of basic microbiology

authors： Jarling M,Cauvet T,Grundmeier M,Kuhnert K,Pape H

更新日期：2004-01-01 00:00:00

abstract：:L-form strains of Proteus mirabilis and Escherichia coli lacking the cell wall represent an alternative prokaryotic cell system for the production of recombinant proteins (KLESSEN et al. 1988, LAPLACE et al. 1988a, 1989b). We could demonstrate that they are also able to synthesize the enzyme penicillin G acylase (PAC)...

journal_title：Journal of basic microbiology

authors： Gumpert J,Cron H,Plapp R,Niersbach H,Hoischen C

更新日期：1996-01-01 00:00:00

abstract：:A gene encoding an organic solvent-stable protease was amplified from Pseudomonas aeruginosa strain K by polymerase chain reaction using consensus primers based on multiple sequence alignment of alkaline and metalloprotease genes from Pseudomonas species. The gene, which consisted of 1440 bp nucleotides and deduced 47...

journal_title：Journal of basic microbiology

authors： Rahman RN,Geok LP,Wong CF,Basri M,Salleh AB

更新日期：2010-04-01 00:00:00

abstract：:Proteus mirabilis (P. mirabilis) is one of Gram-negative pathogens encountered in clinical specimens. A clinical isolate (TRP41) of P. mirabilis was isolated from a Turkish patient in Turkey. The isolate was identified using the API 32GN system and 16S rRNA gene sequencing and it was found resistant to ampicillin/sulb...

journal_title：Journal of basic microbiology

authors： Cicek AC,Duzgun AO,Saral A,Sandalli C

更新日期：2014-10-01 00:00:00

abstract：:The presence of the ica loci and adhesins genes in clinical Staphylococcus aureus strains were considered important factors of virulence. In this study, 46 strains of Staphylococcus aureus were isolated from auricular infection, and were investigated for slime production using Congo Red Agar method (CRA). In order to ...

journal_title：Journal of basic microbiology

authors： Zmantar T,Chaieb K,Makni H,Miladi H,Abdallah FB,Mahdouani K,Bakhrouf A

更新日期：2008-08-01 00:00:00

abstract：:The objective was to screen for and isolate a novel enzyme with the specific activity of a Δ6-fatty acid desaturase from Rhizopus oryzae. In this study, R. oryzae was identified as a novel fungal species that produces large amounts of γ-linolenic acid. A full-length cDNA, designated here as RoD6D, with high homology t...

journal_title：Journal of basic microbiology

authors： Zhu Y,Zhang BB

更新日期：2013-09-01 00:00:00

abstract：:A comparative study of the wild type A. nidulans and a mutant strain aco-T69 (lacking conidia and cleistothecia) revealed the better production of beta-1,4-glucosidase in the former. The relative distribution of the enzyme levels in various morphological structures viz. somatic hyphae, spores and cleistothecia also sh...

journal_title：Journal of basic microbiology

authors： Sharma S,Bagga PS,Sandhu DK

更新日期：1990-01-01 00:00:00

abstract：:Yeasts form a significant and diverse part of the phyllosphere microbiota. Some yeasts that inhabit plants have been found to exhibit extracellular enzymatic activities. The aim of the present study was to investigate the ability of yeasts isolated from leaves, fruits, and blossoms of fruit trees cultivated in Southwe...

journal_title：Journal of basic microbiology

authors： Molnárová J,Vadkertiová R,Stratilová E

更新日期：2014-07-01 00:00:00

abstract：:Phytochromes are photoreceptor proteins involved in the detection of the red and far-red regions of the visible light spectrum. Fungal phytochromes are hybrid histidine kinases with a conserved domain architecture composed of an N-terminal photosensory module and a C-terminal regulatory output module that includes the...

journal_title：Journal of basic microbiology

authors： Lavín JL,Ramírez L,Pisabarro AG,Oguiza JA

更新日期：2015-09-01 00:00:00

abstract：:The ability of four strains of Aspergillus parasiticus (IMI 301,001) and two strains of Aspergillus flavus (IMI 300,998) to produce aflatoxins on Nigerian groundnuts under varying conditions of temperature was studied. While all the A. parasiticus strains produced the four major aflatoxins (B1, B2, G1, G2), only aflat...

journal_title：Journal of basic microbiology

authors： Ogundero VW

更新日期：1987-01-01 00:00:00

abstract：:Candida albicans supernatants contain a mixture of autoregulatory alcohols. In vitro, when added individually or in combination, these alcohols inhibit the yeast to filamentous form conversion. Here we evaluate the in vivo effect of the exogenous administration of a Cocktail solution simulating the composition of alco...

journal_title：Journal of basic microbiology

authors： Martins M,Lazzell AL,Lopez-Ribot JL,Henriques M,Oliveira R

更新日期：2012-08-01 00:00:00

abstract：:Optimization of indigo production process from indole using a newly isolated phenol-degrading bacterial strain was performed by Plackett-Burman design and response surface methodology. The strain designated as QM was identified as Pseudomonas sp. according to 16S rDNA analysis. Spectrum analysis of indole biotransform...

journal_title：Journal of basic microbiology

authors： Qu Y,Ma Q,Zhang X,Zhou H,Li X,Zhou J

更新日期：2012-12-01 00:00:00

abstract：:Screening new organisms for antibacterial activity and searching for new antibacterial drugs is important due to the constant generation of new antibiotic-resistant strains of pathogenic bacteria. An E. coli broth microdilution test was used to evaluate the results of the Vibrio fischeri bioluminescence test in five o...

journal_title：Journal of basic microbiology

authors： Janes D,Umek A,Kreft S

更新日期：2006-01-01 00:00:00

abstract：:Glucocorticosteroids such as dexamethasone have polluted hospital wastewater, urban sewage, and river water in varying degrees. However, dexamethasone degradation by bioremediation technology is less understood. This study aims to isolate bacteria that could degrade dexamethasone and to identify their degradation char...

journal_title：Journal of basic microbiology

authors： Yi W,Zhibang Y,Lili Z,Zhongquan S,Lianju M,Ziwei T,Renju J

更新日期：2015-02-01 00:00:00

abstract：:Epiphytic microbes are common inhabitants of freshwater submerged macrophytes, which play an important role in aquatic ecosystems. An important precondition for studying the epiphytic microbes is having an effective method of separating the attached microbes from the host macrophytes. We developed an ultrasound-based ...

journal_title：Journal of basic microbiology

authors： Cai X,Gao G,Yang J,Tang X,Dai J,Chen D,Song Y

更新日期：2014-07-01 00:00:00

abstract：:An extracellular endoglucanase (EG) that catalyzes the hydrolysis of carboxy-methyl cellulose (CMC) as substrate was purified to homogeneity from the soft-rot causing oomycete P. myriotylum with maximum EG production observed in presence of 1% (w/v) sucrose. The enzyme designated PmEG was observed to be monomeric with...

journal_title：Journal of basic microbiology

authors： Geethu C,Nair RA

更新日期：2014-12-01 00:00:00

abstract：:Abscisic acid (ABA) plays important roles in many aspects of plant growth and development. Botrytis cinerea TB-3-H8, a high-yield strain of ABA, was used to elucidate the molecular mechanisms of ABA production in the present work. cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique was applied to isolate...

journal_title：Journal of basic microbiology

authors： Gong T,Shu D,Zhao M,Zhong J,Deng HY,Tan H

更新日期：2014-03-01 00:00:00

abstract：:The impact of increasing Cd2+ exposure on the quality and quantity of siderophores produced by a plant growth promoting Pseudomonas fulva strain was tested to gain insight into the degree of change. P. fulva was cultured in the increasing concentrations of Cd2+ (0, 0.5, 1.0, 2.0 mM). The secreted siderophores were sep...

journal_title：Journal of basic microbiology

authors： Thiem D,Złoch M,Gadzała-Kopciuch R,Szymańska S,Baum C,Hrynkiewicz K

更新日期：2018-07-01 00:00:00

abstract：:Intracellular aminopeptidase synthesized by a soil strain of Pseudomonas sp. was purified 323-fold using the following procedure: saturation with ammonium sulfate, separation by preparative electrophoresis, anion-exchange chromatography and gel filtration chromatography. Molecular weight of the enzyme determined accor...

journal_title：Journal of basic microbiology

authors： Jankiewicz U,Bielawski W

更新日期：2002-01-01 00:00:00

abstract：:Cold acclimation is an important process to increase freezing tolerance for over-winter survival in many organisms. The apoplastic area is very important in cold acclimation. Two-dimensional electrophoresis was used to identify apoplastic proteins involved in the cold acclimation process of the filamentous fungus Magn...

journal_title：Journal of basic microbiology

authors： Li J,Jia B,Liang X,Liu J,Wang Y,Liang X,Yan H,Wang Y,Zhang S

更新日期：2014-04-01 00:00:00

abstract：:The phylogeny of the latest recognized domain, Archaea, is still complicated and it is largely based on environmental sequences. A culture independent molecular phylogenetic analysis revealed high Archaea diversity in a terrestrial hot spring, village Varvara, Bulgaria. A total of 35 archaeal operational taxonomic uni...

journal_title：Journal of basic microbiology

authors： Ivanova I,Atanassov I,Lyutskanova D,Stoilova-Disheva M,Dimitrova D,Tomova I,Derekova A,Radeva G,Buchvarova V,Kambourova M

更新日期：2011-04-01 00:00:00

abstract：:Total cellular proteins and fatty acid composition profiles of mid-log phase cells of Salmonella enterica serovar Typhimurium grown at 8, 25, 37 or 42 °C were separated by 2D-PAGE and FAME analysis. Growth temperature associated protein expression can be grouped into 3 thermal classes which include proteins whose expr...

journal_title：Journal of basic microbiology

authors： Balamurugan S,Dugan ME

更新日期：2010-12-01 00:00:00

abstract：:A set of 50 randomly chosen fungal strains belonging to different basidiomycete species was tested for H2O2 and ligninolytic enzyme production and for decolorization of synthetic dyes Orange G and Remazol Brilliant Blue R. The decolorization capacity of individual strains was influenced by the level of H2O2 and laccas...

journal_title：Journal of basic microbiology

authors： Eichlerová I,Homolka L,Lisá L,Nerud F

更新日期：2006-01-01 00:00:00

abstract：:From seawater and the intestines of benthonic organisms collected from the Beagle Channel, Argentina, 230 marine bacteria were isolated. Cultivable bacteria were characterized and classified as psychrotolerant, whereas few isolates were psychrophiles. These isolates were capable of producing proteases at 4 and 15 °C u...

journal_title：Journal of basic microbiology

authors： Cristóbal HA,López MA,Kothe E,Abate CM

更新日期：2011-12-01 00:00:00

abstract：:Amino acid and protein biosynthesis requires a number of high energy phosphate bonds and includes a dual energy cost for the synthesis of chemical intermediates during the fueling reactions and the conversion of precursor molecules to final products. One popular hypothesis is that the proteins encoded by putative high...

journal_title：Journal of basic microbiology

authors： Sarkar I,Tisa LS,Gtari M,Sen A

更新日期：2018-02-01 00:00:00

abstract：:Intracellular proteolytic activity was detected in the haloalkaliphilic archaeon Natronococcus occultus during the stationary phase of cultures grown in complete medium and during carbon and nitrogen starvation. Puromycin prevented the occurrence of proteolytic activity in starved cells, suggesting that de novo synthe...

journal_title：Journal of basic microbiology

authors： Herrera Seitz K,Studder C,Sanchez J,De Castro R

更新日期：1997-01-01 00:00:00

abstract：:The yeast Cryptococcus sp. UFMG-Y28 can utilize benzonitrile as a nitrogen and possible additional carbon source. The kinetics of growth on Yeast Carbon Base (YCB) added of benzonitrile as sole nitrogen source showed that benzonitrile was metabolized to benzoic acid and ammonia. Liquid chromatography analysis indicate...

journal_title：Journal of basic microbiology

authors： Rezende RP,Dias JC,Ferraz V,Linardi VR

更新日期：2000-01-01 00:00:00

abstract：:The prokaryotic diversity in two brackish lakes (Sayram Lake and Chaiwopu Lake) was investigated by constructing bacterial and archaeal clone libraries of 16S rRNA genes. Bacterial clones from Sayram Lake were classified into six phyla (Proteobacteria, Verrucomicrobia, Bacteroidetes, Planctomycetes, Acidobacteria, Act...

journal_title：Journal of basic microbiology

authors： Zeng J,Deng LJ,Lou K,Zhang T,Yang HM,Shi YW,Lin Q

更新日期：2014-06-01 00:00:00

abstract：:This study evaluated ethanol fermentation and its correlation with glutathione (GSH) synthesis under various cadmium-conditions with different metal ions and nitrogen sources. We found that corn steep liquor (CSL) and yeast extract have differential roles to play in GSH accumulation in cell even though both of them co...

journal_title：Journal of basic microbiology

authors： Xu Q,Wu M,Hu J,Gao MT

更新日期：2016-01-01 00:00:00