Abstract:
:The phylogeny of the latest recognized domain, Archaea, is still complicated and it is largely based on environmental sequences. A culture independent molecular phylogenetic analysis revealed high Archaea diversity in a terrestrial hot spring, village Varvara, Bulgaria. A total of 35 archaeal operational taxonomic units (OTUs) belonging to three of the classified five Archaea phyla were identified. Most of the sequences were affiliated with the phylum Crenarchaeota (23), grouped in four branches. The rest of the sequences showed highest similarity to the unidentified archaeal clones (9), Euryarchaeota (2), and "Korarchaeota " (1). Eight (23%) of the sequenced 16S rDNAs didn't have known close relatives and represented new and diverse OTUs, four of them forming a new archaeal subgroup without close described sequences or culturable relatives. A sequence affiliated with "Korarchaeota " showed low similarity (90%) to the closest neighbor and both sequences formed unique branch in this phylum. Consequently, the constructed archaeal libraries are characterized by (1) high proportion of OTUs representing uncultivated archaeal phylogroups, (2) the abundance of novel phylotype sequences, (3) the presence of high proportions of Crenarchaeota phylotypes unrelated to cultivated organisms and (4) the presence of a sequence only distantly related to "Korarchaeota " phylum.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Ivanova I,Atanassov I,Lyutskanova D,Stoilova-Disheva M,Dimitrova D,Tomova I,Derekova A,Radeva G,Buchvarova V,Kambourova Mdoi
10.1002/jobm.201000160subject
Has Abstractpub_date
2011-04-01 00:00:00pages
163-72issue
2eissn
0233-111Xissn
1521-4028journal_volume
51pub_type
杂志文章abstract::Studies on Acidithiobacillus ferrooxidans accepting electrons from Fe(II) have previously focused on cytochrome c. However, we have discovered that, besides cytochrome c, type IV pili (Tfp) can transfer electrons. Here, we report conduction by Tfp of A. ferrooxidans analyzed with a conducting-probe atomic force micros...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201200300
更新日期:2014-03-01 00:00:00
abstract::The biological treatment of spent wash from molasses distilleries was investigated. Analysis of raw spent wash showed it to be a recalcitrant waste, with a high COD of 85,170 mg/l and containing inhibitory phenolic compounds. Reverse phase thin layer chromatography identified gallic and vanillic acid present in spent ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620350504
更新日期:1995-01-01 00:00:00
abstract::Open reading frame17 (Bm17) of Bombyx mori nucleopolyhedrovirus is a highly conserved gene in lepidopteran nucleopolyhedroviruses, suggesting that it performs an important role in the virus life cycle whose function is unknown. In this report, we describe the characterization of Bm17. Reversed transcriptive-PCR (RT-PC...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201200165
更新日期:2013-10-01 00:00:00
abstract::Citrobacter freundii is a worldwide emerging nosocomial pathogen with escalating incidence of multidrug resistance. Citrobacter freundii exists in natural environment, especially in health care settings and is difficult to eradicate. Phage therapy is considered as an alternative way of controlling bacterial infections...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201200710
更新日期:2014-06-01 00:00:00
abstract::A set of 50 randomly chosen fungal strains belonging to different basidiomycete species was tested for H2O2 and ligninolytic enzyme production and for decolorization of synthetic dyes Orange G and Remazol Brilliant Blue R. The decolorization capacity of individual strains was influenced by the level of H2O2 and laccas...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200610064
更新日期:2006-01-01 00:00:00
abstract::Yeast<-->mycelium morphological transitions of Aureobasidium pullulans are influenced by numerous environmental factors. In general, changes in the glutathione (GSH) metabolism of dimorphic fungi may lead to alterations in the reduced thiol status of the cells that are hypothesised to initialise morphological transiti...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/1521-4028(200105)41:2<131::AID-JOBM131>3.0
更新日期:2001-01-01 00:00:00
abstract::The presence of the ica loci and adhesins genes in clinical Staphylococcus aureus strains were considered important factors of virulence. In this study, 46 strains of Staphylococcus aureus were isolated from auricular infection, and were investigated for slime production using Congo Red Agar method (CRA). In order to ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200700289
更新日期:2008-08-01 00:00:00
abstract::The objective was to screen for and isolate a novel enzyme with the specific activity of a Δ6-fatty acid desaturase from Rhizopus oryzae. In this study, R. oryzae was identified as a novel fungal species that produces large amounts of γ-linolenic acid. A full-length cDNA, designated here as RoD6D, with high homology t...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201200189
更新日期:2013-09-01 00:00:00
abstract::The microbial diversity of production waters of five Tunisian oilfields was investigated using Single Strand Conformation Polymorphism (SSCP) technique followed by cloning-sequencing. Dynamics of bacterial populations in production waters collected from four wellheads were also evaluated. For all production water samp...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201100528
更新日期:2013-01-01 00:00:00
abstract::Pichia pastoris expression system was widely used for producing heterologous proteins. Some researches revealed that the increase in the copy number could improve the expression of foreign genes in P. pastoris. Hundreds or thousands of antibiotic-resistance recombinants need to be screened because the frequency of mul...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201400208
更新日期:2014-12-01 00:00:00
abstract::Spore (akinete) formation in the heterocystous and branched filamentous cyanobacterium Fischerella muscicola involves a significant increase in cell size and formation of several endospores in each of the cells. In present study, the physico-chemical factors (pH, light sources, nutrient deficiency, nitrogen sources, c...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201800161
更新日期:2018-08-01 00:00:00
abstract::Heavy metal tolerant and resistant strains of streptomycetes isolated from a former uranium mining site were screened for their superoxide dismutase expression. From the strains tolerating high concentrations of different heavy metals, one was selected for its tolerance of concentrations of heavy metals (Ni, Cu, Cd, C...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200610213
更新日期:2007-02-01 00:00:00
abstract::A novel moderately thermophilic, Gram-positive, endospore-forming, rod-shaped, motile bacteria and alkaline active xylanase producing strain D1021(T) , was isolated from Kaynarca hot spring in the province of Izmir, Turkey and was characterized in order to determine its phylogenetic position. Growth was observed at 35...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201100638
更新日期:2013-05-01 00:00:00
abstract::An outbreak of serious mortality among the cultured cobia Rachycentron canadum (weighing 3 kg) characterized by the presence of whitish granulomatous deposits on the kidney, liver and spleen occurred in July of 2000 in Taiwan. A non-motile strain CP1 was isolated from kidney and/or liver on tryptic soy agar and/or bra...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200310301
更新日期:2003-01-01 00:00:00
abstract::The possibility of associating starch degradation with bacterial beta-glucuronidase expression was examined. We proved that starving, in starch medium, amylase-negative Escherichia coli (M94) which has constitutive beta-glucuronidase greatly reduces (p < 0.01) its background activity, but the addition of both cell-fre...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Shigella adhesion to host cells is a transitional stage from an extracellular to an intracellular environment. However, the dynamic adaptations of Shigella during adhesion are poorly understood. To address this, we performed the first transcriptome analysis of Shigella flexneri 2457T during adhesion. A total of 1,757 ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201400414
更新日期:2015-02-01 00:00:00
abstract::Rice cultivation is the main method for mudflat reclamation. However, changes in the community structure of microbes involved in nitrogen (N) cycling in response to mudflat reclamation via rice cultivation remain poorly understood. This study used quantitative polymerase chain reaction to characterize the distribution...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201800660
更新日期:2019-05-01 00:00:00
abstract::Efficiency of solid and liquid inocula and their use for spawn production were compared so that improved cultivation conditions for the edible mushroom Pleurotus ostreatoroseus could be tested. Solid and liquid inocula were prepared respectively with Potato Dextrose Agar (PDA) and Liquid Potato Dextrose (LPD). Wheat g...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/1521-4028(200203)42:1<37::AID-JOBM37>3.0.C
更新日期:2002-01-01 00:00:00
abstract::The prokaryotic diversity in two brackish lakes (Sayram Lake and Chaiwopu Lake) was investigated by constructing bacterial and archaeal clone libraries of 16S rRNA genes. Bacterial clones from Sayram Lake were classified into six phyla (Proteobacteria, Verrucomicrobia, Bacteroidetes, Planctomycetes, Acidobacteria, Act...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201300187
更新日期:2014-06-01 00:00:00
abstract::The metabolization of L-carnitine was studied using whole cells of Escherichia coli 044 K74. It showed features of an epigenetical control. L-carnitine and crotonobetaine were able to induce the carnitine-reducing system. Oxygen and nitrate as electron acceptors, gamma-butyrobetaine as final product of carnitine trans...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620270303
更新日期:1987-01-01 00:00:00
abstract::Rock phosphate effect on English mint (Mentha piperita L.) grown on steamed perlite:vermiculite (1:1, v:v) substrate, with and without rock phosphate, was evaluated in greenhouse experiments. Five treatments were carried out by inoculation with an arbuscular mycorrhizal fungus Glomus mosseae and a phosphorus solubiliz...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200410409
更新日期:2005-01-01 00:00:00
abstract::Proteus mirabilis (P. mirabilis) is one of Gram-negative pathogens encountered in clinical specimens. A clinical isolate (TRP41) of P. mirabilis was isolated from a Turkish patient in Turkey. The isolate was identified using the API 32GN system and 16S rRNA gene sequencing and it was found resistant to ampicillin/sulb...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201300264
更新日期:2014-10-01 00:00:00
abstract::Two shuttle vectors named pRC52 (10.7 Kb) and pRK52 (12.2 Kb) carrying chloramphenicol (Cm) and chloramphenicol plus kanamycin (Km) resistance genes, respectively, were constructed by fusion of a cryptic plasmid pBL13869 (replicon pBL1, 5.8 Kb) from Brevibacterium lactofermentum ATCC13869 with pBR328 E. coli plasmid. ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Plant endophytes are rich in secondary metabolites and are widely used in medicine, chemical, food, agriculture, and other fields. Here, an endophytic fungus is isolated from Ginkgo biloba L. leaves and identified as Alternaria brassicae GL07 through genotypic characterizations. It can produce fruity scented volatiles...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201800729
更新日期:2019-07-01 00:00:00
abstract::Endophytes represent a hidden world within plants. Almost all plants that are studied harbor one or more endophytes, which help their host to survive against pathogens and changing adverse environmental conditions. Fungal and bacterial endophytes with distinct ecological niches show important biological activities and...
journal_title:Journal of basic microbiology
pub_type: 杂志文章,评审
doi:10.1002/jobm.201700275
更新日期:2017-10-01 00:00:00
abstract::To understand the physiology of non-differentiating bacteria exposed to nutrient deprivation and stress, various approaches have been employed in combination with detailed analysis of protein synthesis pattern. In this study, separation of proteins from clarified cell extracts of Pseudomonas aeruginosa PAO1 grown unde...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200390002
更新日期:2003-01-01 00:00:00
abstract::The depolymerization of humic acids (HAs) obtained from low-rank coal (lignite) to fulvic acids (FAs) was investigated in a cell-free system (in vitro) using manganese peroxidase (MnP) from the white-rot fungus Clitocybula dusenii b11. MnP was produced in surface cultures of C. dusenii which were induced with manganes...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::An extracellular endoglucanase (EG) that catalyzes the hydrolysis of carboxy-methyl cellulose (CMC) as substrate was purified to homogeneity from the soft-rot causing oomycete P. myriotylum with maximum EG production observed in presence of 1% (w/v) sucrose. The enzyme designated PmEG was observed to be monomeric with...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201400323
更新日期:2014-12-01 00:00:00
abstract::Swainsonine is a polyhydroxylated indolizidine alkaloid having anticancer, antimetastatic, antiproliferative and immunomodulatory activities and also potential therapeutic applications against AIDS. In the present study, ten isolates of M. anisopliae were screened and enzyme assayed for the production of swainsonine i...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201100255
更新日期:2012-10-01 00:00:00
abstract::The infective capacities of the nitrogen fixing Actinomycete Frankia and arbuscular mycorrhizal fungi from soils near watercourses, along a vegetation gradient, were studied using plant bioassays. Frankia and arbuscular mycorrhizas capable of infecting Discaria trinervis were found at seventeen sites sampled. More spe...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200510095
更新日期:2006-01-01 00:00:00