A simple strategy for the generation of multi-copy Pichia pastoris with the efficient expression of mannanase.

abstract：:Removal of heavy metals from food material by growing micro-organisms is limited by the toxicity to cells. In this study, different preincubation treatments were investigated to analyze their effects on cadmium resistance and removal ability of Pichia kudriavzevii A16 and Saccharomyces cerevisiae CICC1211. Sucrose pre...

journal_title：Journal of basic microbiology

authors： Zhang D,Ma N,Guo Z,Chen P,Ma R,Sun X,Wang D,Wang J,Xu Y

更新日期：2019-09-01 00:00:00

abstract：:Considered are our own data and those found in literature on the properties of yeast mutants impaired in their ability to utilize methanol as sole carbon and energy source; hypotheses about the role of alcohol oxidase and citrate synthase in biogenesis of peroxisomes are proposed. It has been proved that formaldehyde ...

journal_title：Journal of basic microbiology

authors： Sibirny AA,Titorenko VI,Gonchar MV,Ubiyvovk VM,Ksheminskaya GP,Vitvitskaya OP

更新日期：1988-01-01 00:00:00

abstract：:Bacteria capable of methylating Hg2+ have been isolated from sediment, water, soil and the gastrointestinal tract of humans. However, very little is known about the physiology and genetics of the mechanisms controlling Hg2+ methylation. Mercury methylation can be either chromosomal or plasmid-encoded in bacteria. In a...

journal_title：Journal of basic microbiology

authors： Trevors JT

更新日期：1986-01-01 00:00:00

abstract：:A comparative study of the wild type A. nidulans and a mutant strain aco-T69 (lacking conidia and cleistothecia) revealed the better production of beta-1,4-glucosidase in the former. The relative distribution of the enzyme levels in various morphological structures viz. somatic hyphae, spores and cleistothecia also sh...

journal_title：Journal of basic microbiology

authors： Sharma S,Bagga PS,Sandhu DK

更新日期：1990-01-01 00:00:00

abstract：:Aspergillus nidulans produces sterigmatocystin, a secondary metabolite mycotoxin, for the protection of its reproductive structures. Previous studies on grazing behavior of fungivore arthropods, regulation of sexual development, and secondary metabolite biosynthesis have revealed the association of sterigmatocystin bi...

journal_title：Journal of basic microbiology

authors： Ámon J,Keisham K,Bokor E,Kelemen E,Vágvölgyi C,Hamari Z

更新日期：2018-07-01 00:00:00

abstract：:An extracellular cold-adapted alkaline lipase from the psychrotrophic Yersinia enterocolitica strain KM1 was purified 26-fold to homogeneity. The enzyme was active over a broad range spanning 0-60 °C with an optimum activity at 37 °C, and it was found to be alkaline-preferring with an optimum activity at pH 9.0. The m...

journal_title：Journal of basic microbiology

authors： Ji X,Chen G,Zhang Q,Lin L,Wei Y

更新日期：2015-06-01 00:00:00

abstract：:Heterotrophic bacteria (11 genera, 14 species, 25 putative strains) were isolated from drinking water, identified either biochemically or by partial 16s rDNA gene sequencing and their adherence characteristics were determined by two methods: i. thermodynamic prediction of adhesion potential by measuring hydrophobicity...

journal_title：Journal of basic microbiology

authors： Simões LC,Simões M,Oliveira R,Vieira MJ

更新日期：2007-04-01 00:00:00

abstract：:This study investigates the anticancer cytotoxic mechanism of action of benzoyloxy-ethyl-carbamic acid (BECA) produced by Streptomyces globosus VITLGK011. Flow cytometry analysis confirmed that BECA (at IC50 : 3.12 µg/ml) treatment for 24 h induced apoptosis in 60% of cells. Schrodinger Maestro tools such as QikProp a...

journal_title：Journal of basic microbiology

authors： Lokesh R,Jeyakanthan J,Kannabiran K

更新日期：2020-11-01 00:00:00

abstract：:Helicobacter pylori exhibit morphology convertion in a spiral or coccoid form. This study aims to reveal the impact of coccoid H. pylori on the proliferation and apoptosis of gastric epithelial cells. The cagA and vacA genes of H. pylori were detected by semi-quantitative RT-PCR. Proliferation and apoptosis were analy...

journal_title：Journal of basic microbiology

authors： Li N,Han L,Chen J,Lin X,Chen H,She F

更新日期：2013-02-01 00:00:00

abstract：:Studies were carried out to screen twelve strains of Lentinus edodes for their efficiency to grow on toxic agro-industrial residues of coffee industry in solid state cultivation (SSC). Based on best mycelial growth (7.57 mm/day) and biomass production (48.78 mg/plate in 12 days at 24 degrees C) in coffee husk extract ...

journal_title：Journal of basic microbiology

authors： Leifa F,Pandey A,Soccol CR

更新日期：2000-01-01 00:00:00

abstract：:The biological treatment of spent wash from molasses distilleries was investigated. Analysis of raw spent wash showed it to be a recalcitrant waste, with a high COD of 85,170 mg/l and containing inhibitory phenolic compounds. Reverse phase thin layer chromatography identified gallic and vanillic acid present in spent ...

journal_title：Journal of basic microbiology

authors： Fitzgibbon FJ,Nigam P,Singh D,Marchant R

更新日期：1995-01-01 00:00:00

abstract：:The high-level expression of the xylanase GH11 gene from Aspergillus niger IA-001 called xynB was successfully completed in Pichia pastoris. The xynB gene encoding a mature xylanase of 225 amino acid was subcloned into the pPICZαA vector and was transformed into P. pastoris X-33 under the control of the alcohol oxidas...

journal_title：Journal of basic microbiology

authors： Fang W,Gao H,Cao Y,Shan A

更新日期：2014-07-01 00:00:00

abstract：:Biofilms are means of protection to bacteria against antibiotics and antibodies. Catheters and others tube devices used by patients are prone to accumulation of thick layers of biofilms as hiding place for etiologic agents, resulting in substantial morbidity and mortality. Methicillin-resistant Staphylococcus aureus (...

journal_title：Journal of basic microbiology

authors： Mirani ZA,Jamil N

更新日期：2011-04-01 00:00:00

abstract：:This paper focuses on isolation and identification of airborne and waterborne fungi from different parts of Terkos Lake located in Istanbul (Turkey). The quantitative and qualitative fungal composition of the air and water of the Lake was surveyed monthly for a year (August 2000-July 2001). Water samples were taken at...

journal_title：Journal of basic microbiology

authors： Asan A,Kirgiz T,Sen B,Camur-Elipek B,Guner U,Guher H

更新日期：2003-01-01 00:00:00

abstract：:The nourseothricin-producing S. noursei strain JA 3890b possessed a high degree of resistance to its own antibiotic when grown in submerged cultures started from mycelium samples as inocula. In contrast, both the outgrowth of spores and the development of surface colonies from mycelium samples were severely inhibited ...

journal_title：Journal of basic microbiology

authors： Röder B,Plonka G,Bormann EJ,Gräfe U

更新日期：1985-01-01 00:00:00

abstract：:In the past years, research on the flagellate unicellular alga Chlamydomonas reinhardtii has entered a new era based on the availability of its complete genome. Since this green alga can be grown relatively easy in a short time-range, sufficient biological material is available to efficiently establish biochemical pur...

journal_title：Journal of basic microbiology

authors： Wagner V,Boesger J,Mittag M

更新日期：2009-02-01 00:00:00

abstract：:Mercury tolerant bacteria Pseudarthrobacter oxydans strain MM20 and Pseudomonas frederiksbergensis strain SS18 were isolated from the tundra ecosystem of Ny-Ålesund, Svalbard, where commercial exploitation of the coal existed till 1960s. Minimum inhibitory concentration (MIC), mercury removal, mercury biosorption, and...

journal_title：Journal of basic microbiology

authors： Mechirackal Balan B,Shini S,Krishnan KP,Mohan M

更新日期：2018-04-01 00:00:00

abstract：:Microorganisms such as bacteria and molds produce an enormous variety of volatile metabolites. To determine whether typical microbial volatile metabolites can be used as indicator compounds for the detection of hidden mold in indoor environments, we examined 14 typical indoor fungal strains for their growth rates and ...

journal_title：Journal of basic microbiology

authors： Schuchardt S,Kruse H

更新日期：2009-08-01 00:00:00

abstract：:The phylogeny of the latest recognized domain, Archaea, is still complicated and it is largely based on environmental sequences. A culture independent molecular phylogenetic analysis revealed high Archaea diversity in a terrestrial hot spring, village Varvara, Bulgaria. A total of 35 archaeal operational taxonomic uni...

journal_title：Journal of basic microbiology

authors： Ivanova I,Atanassov I,Lyutskanova D,Stoilova-Disheva M,Dimitrova D,Tomova I,Derekova A,Radeva G,Buchvarova V,Kambourova M

更新日期：2011-04-01 00:00:00

abstract：:Calcium signaling is one of the most important signaling-pathways in all eukaryotes. One important target activated by an increased intracellular calcium concentration via calmodulin is the protein phosphatase calcineurin, which is composed of a catalytic subunit (calcineurin A) and a regulatory subunit (calcineurin B...

journal_title：Journal of basic microbiology

authors： Thewes S,Schubert SK,Park K,Mutzel R

更新日期：2014-06-01 00:00:00

abstract：:alpha-Aminodipate, an intermediate of the lysine biosynthetic pathway of fungi, or lysine when used as the sole nitrogen source in the medium was growth inhibitory and toxic to Saccharomyces cerevisiae. The fission yeast Schizosaccharomyces pombe and pathogenic fungi Candida albicans, Filobasidiella neoformans and Asp...

journal_title：Journal of basic microbiology

authors： Ye ZH,Garrad RC,Winston MK,Bhattacharjee JK

更新日期：1991-01-01 00:00:00

abstract：:A simple photometric method for the determination of the agglutination rate of cells by lectins in a continuously stirred suspension is presented. Besides the agglutination rate the method allows the estimation of an average degree of agglutination, i.e. the number of cells per aggregate. The influence of the Con A-co...

journal_title：Journal of basic microbiology

authors： Flemming C,Gabert A,Flemming I

更新日期：1985-01-01 00:00:00

abstract：:107 species and 8 species varieties belonging to 44 genera were collected from combine harvester wheat and sorghum dusts (35 genera and 91 species + 4 varieties) and from the atmosphere of their hay sites (26 genera and 69 species + 4 varieties) on glucose- and cellulose-Czapek's Dox agar at 28 degrees C and 45 degree...

journal_title：Journal of basic microbiology

authors： Abdel-Hafez SI,Moubasher AH,Shoreit AA,Ismail MA

更新日期：1990-01-01 00:00:00

abstract：:The effect of high Cu(II) concentrations on superoxide dismutase (SOD) and catalase (CAT) activity in Candida fukuyamaensis RCL-3 and Rhodotorula mucilaginosa RCL-11, previously isolated from a copper filter at a mine plant in Argentina, was studied. Addition of 0.1, 0.2 and 0.5 mM Cu(II) to the culture medium increas...

journal_title：Journal of basic microbiology

authors： Villegas LB,Amoroso MJ,de Figueroa LI

更新日期：2009-08-01 00:00:00

abstract：:Chiral intermediates have wide application and high demand in pharmaceutical, agricultural, and other biotechnological industries for the preparation of bulk drug substances or fine chemicals. (S)-1-(1-napthyl) ethanol is an important synthetic intermediate of mevinic acid analog and a potential inhibitor of 3-hydroxy...

journal_title：Journal of basic microbiology

authors： Ranjan P,Pandey A,Binod P

更新日期：2017-09-01 00:00:00

abstract：:Indigenous thermophilic hydrocarbon degraders are of special significance for the bioremediation of oil-contaminated desert soils with ambient temperature of 45-50 degrees C. The first objective of this study was to demonstrate the hydrocarbon-degrading capability of Nocardia otitidiscaviarum TSH1 (DSM 45,036) which g...

journal_title：Journal of basic microbiology

authors： Zeinali M,Vossoughi M,Ardestani SK,Babanezhad E,Masoumian M

更新日期：2007-12-01 00:00:00

abstract：:In the course of a screening for extracellular inhibitors of 8 different proteinases 140 actinomycete and 150 mould strains were examined. 130 fungal and 50 actinomycete strains were similarly tested for the production of extracellular inhibitors of dihydrofolate reductase (DHFR) and alkaline phosphatase (APh). Most o...

journal_title：Journal of basic microbiology

authors： Angelov T,Angelova B,Kourteva Y,Stephanova L,Ayramova T,Peretz V,Konova O

更新日期：1989-01-01 00:00:00

abstract：:Amino acid and protein biosynthesis requires a number of high energy phosphate bonds and includes a dual energy cost for the synthesis of chemical intermediates during the fueling reactions and the conversion of precursor molecules to final products. One popular hypothesis is that the proteins encoded by putative high...

journal_title：Journal of basic microbiology

authors： Sarkar I,Tisa LS,Gtari M,Sen A

更新日期：2018-02-01 00:00:00

abstract：:Intracellular aminopeptidase synthesized by a soil strain of Pseudomonas sp. was purified 323-fold using the following procedure: saturation with ammonium sulfate, separation by preparative electrophoresis, anion-exchange chromatography and gel filtration chromatography. Molecular weight of the enzyme determined accor...

journal_title：Journal of basic microbiology

authors： Jankiewicz U,Bielawski W

更新日期：2002-01-01 00:00:00

abstract：:Propidium iodide (PI) uptake and salt tolerance assays were used to investigate the mechanism of antibacterial action of an extract of the leaves of Eremophila duttonii, a traditional Australian medicinal plant previously shown to have potent bactericidal activity against Gram positive bacteria. The extract compromise...

journal_title：Journal of basic microbiology

authors： Tomlinson S,Palombo EA

更新日期：2005-01-01 00:00:00