Stress and development in Dictyostelium discoideum: the involvement of the catalytic calcineurin A subunit.

Abstract:

:Calcium signaling is one of the most important signaling-pathways in all eukaryotes. One important target activated by an increased intracellular calcium concentration via calmodulin is the protein phosphatase calcineurin, which is composed of a catalytic subunit (calcineurin A) and a regulatory subunit (calcineurin B). The importance of calcium and calcineurin for the differentiation and development of the social amoeba Dictyostelium discoideum has already been shown by pharmacological approaches. However, so far only a RNAi-silenced calcineurin B mutant has been investigated on a molecular level. Here, we describe the construction and phenotypic investigation of a RNAi-silenced calcineurin A mutant. Phenotypic aberrations during development resemble those produced by silencing of calcineurin B with ectopic tip formation of the fruiting bodies. Additionally, we tested the response of the mutants under various stress conditions in liquid culture as well as during development. Both, calcineurin A and B RNAi-mutants, are hypersensitive during development towards cation stress. Besides its role in development, calcineurin is thus also involved in the stress response in D. discoideum. Further, our data imply that many functions of calcineurin are conserved among the eukaryotes.

journal_name

J Basic Microbiol

authors

Thewes S,Schubert SK,Park K,Mutzel R

doi

10.1002/jobm.201200574

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

607-13

issue

6

eissn

0233-111X

issn

1521-4028

journal_volume

54

pub_type

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