Abstract:
:A simple photometric method for the determination of the agglutination rate of cells by lectins in a continuously stirred suspension is presented. Besides the agglutination rate the method allows the estimation of an average degree of agglutination, i.e. the number of cells per aggregate. The influence of the Con A-concentration, cell number, temperature, and pH on the agglutination rate of Saccharomyces cerevisiae H155 have been studied. The results are of good reproducibility and, therefore, the methods is suitable to describe interactions between cell-bound receptors and receptor-specific proteins, e.g. lectins and antibodies.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Flemming C,Gabert A,Flemming Idoi
10.1002/jobm.3620250806subject
Has Abstractpub_date
1985-01-01 00:00:00pages
493-501issue
8eissn
0233-111Xissn
1521-4028journal_volume
25pub_type
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