Phenoxyacetic acid induces glutathione-dependent detoxification and depletes the glutathione pool in Penicillium chrysogenum.

Abstract:

:Enzymes of the glutathione-dependent detoxification pathway (glutathione S-transferase and gamma-glutamyl-transpeptidase) were induced, and the glutathione pool was completely depleted by phenoxyacetic acid in Penicillium chrysogenum mycelia incubated for 15 h in a culture medium containing lactose as a carbon source and sodium glutamate as a nitrogen source. A significant increase in both the oxidised glutathione concentrations and the glutathione reductase activities were also observed. 1-Chloro-2,4-dinitrobenzene--a potent substrate and inducer of glutathione S-transferase-initiated very similar physiological changes but no beta-lactam production could be detected in this case. When (NH4)2HPO4 was used as a nitrogen source the penicillin biosynthesis was repressed and the induction of gamma-glutamyltranspeptidase by phenoxyacetic acid was hindered considerably.

journal_name

J Basic Microbiol

authors

Emri T,Pócsi I,Szentirmai A

doi

10.1002/jobm.3620370306

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

181-6

issue

3

eissn

0233-111X

issn

1521-4028

journal_volume

37

pub_type

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