Abstract:
:A subtraction library of group B streptococcus (GBS) strain O9OR with GAS chromosomal DNA (strain SF370) was constructed and more than 100 plasmid clones sequenced. DNA sequences of the plasmid inserts were analyzed using the BLAST gene search. Most inserts had little or no homology to GAS chromosomal DNA and 26 clones from the library had no gene homologues in the gene bank. The majority of genes discovered represented house keeping GBS genes, but several could be considered as possible virulence factors. Inserts from 21 clones were labeled and used as probes for hybridization with GBS DNA fragments separated by pulsed field electrophoresis. A genetic map of GBS strain O9OR was constructed.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Suvorov AN,Ferretti JJdoi
10.1002/jobm.200310307subject
Has Abstractpub_date
2004-01-01 00:00:00pages
66-74issue
1eissn
0233-111Xissn
1521-4028journal_volume
44pub_type
杂志文章abstract::Transposons are a group of mobile genetic elements that are defined as a DNA sequence. Transposons can jump into different places of the genome; for this reason, they are called jumping genes. However, some transposons are always kept at the insertion site in the genome. Most transposons are inactivated and as a resul...
journal_title:Journal of basic microbiology
pub_type: 杂志文章,评审
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abstract::Pichia pastoris expression system was widely used for producing heterologous proteins. Some researches revealed that the increase in the copy number could improve the expression of foreign genes in P. pastoris. Hundreds or thousands of antibiotic-resistance recombinants need to be screened because the frequency of mul...
journal_title:Journal of basic microbiology
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abstract::Trichoderma spp. are versatile beneficial fungi which can stimulate growth and plant resistance to biotic and abiotic stresses. In this study, the potential of Trichoderma isolate in promoting the cucumber growth under salt stress and its possible mechanisms were investigated. Strain Q1 was isolated from the rhizosphe...
journal_title:Journal of basic microbiology
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abstract::In the past years, research on the flagellate unicellular alga Chlamydomonas reinhardtii has entered a new era based on the availability of its complete genome. Since this green alga can be grown relatively easy in a short time-range, sufficient biological material is available to efficiently establish biochemical pur...
journal_title:Journal of basic microbiology
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abstract::In the course of a screening for extracellular inhibitors of 8 different proteinases 140 actinomycete and 150 mould strains were examined. 130 fungal and 50 actinomycete strains were similarly tested for the production of extracellular inhibitors of dihydrofolate reductase (DHFR) and alkaline phosphatase (APh). Most o...
journal_title:Journal of basic microbiology
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abstract::This work reports the production of a novel serine protease enzyme (P. dig-protease) from the fungus Penicillium digitatum. The protease was purified from the culture supernatant to homogeneity using ammonium sulfate precipitation, Sephadex G-150 gel filtration and carboxymethyl-sepharose ion exchange chromatography w...
journal_title:Journal of basic microbiology
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abstract::Removal of heavy metals from food material by growing micro-organisms is limited by the toxicity to cells. In this study, different preincubation treatments were investigated to analyze their effects on cadmium resistance and removal ability of Pichia kudriavzevii A16 and Saccharomyces cerevisiae CICC1211. Sucrose pre...
journal_title:Journal of basic microbiology
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abstract::The diversity of Actinobacteria associated with Paratrechina longicornis, an ant species that prefers a high protein diet, in a subtropical dry forest (Guánica, Puerto Rico) was determined by culture methods and by 16S rDNA clone libraries. The results of both methodologies were integrated to obtain a broader view of ...
journal_title:Journal of basic microbiology
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abstract::Considered are our own data and those found in literature on the properties of yeast mutants impaired in their ability to utilize methanol as sole carbon and energy source; hypotheses about the role of alcohol oxidase and citrate synthase in biogenesis of peroxisomes are proposed. It has been proved that formaldehyde ...
journal_title:Journal of basic microbiology
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abstract::Retraction: The following article from the Journal of Basic Microbiology, "A catechol type siderophore, bacillibactin: biosynthesis, regulation and transport in Bacillus subtilis" by Waseem Raza, Hongsheng Wu, Muhammad Ali Abdullah Shah and Qirong Shen, published online on 11 September 2008 in Wiley InterScience (www....
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abstract::The objective was to screen for and isolate a novel enzyme with the specific activity of a Δ6-fatty acid desaturase from Rhizopus oryzae. In this study, R. oryzae was identified as a novel fungal species that produces large amounts of γ-linolenic acid. A full-length cDNA, designated here as RoD6D, with high homology t...
journal_title:Journal of basic microbiology
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abstract::Acinetobacter spp. are ubiquitous obligate aerobic bacteria which occur mostly as commensals on the skin, and in soil, water and plants' rhizosphere. Though the species in this genus have been implicated as aetiologies in some nosocomial infections, their versatility covers biodegradation or dissolution leading to bio...
journal_title:Journal of basic microbiology
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abstract::The gene mak1FN coding for maltokinase from Actinoplanes missouriensis is located in a cluster similar to glycogen metabolism clusters identified in Streptomyces coelicolor. Sequence comparisons demonstrate that mak1-related genes coding for homologous proteins are present in many bacterial genomes including taxonomic...
journal_title:Journal of basic microbiology
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abstract::Lipases play an important role in the infection process of entomopathogenic fungi by hydrolyzing the ester bonds of lipoproteins, fats and waxes present on the insect surface and in the body. Here we report the purification and characterization of an extracellular lipase from Isaria fumosorosea. The enzyme was purifie...
journal_title:Journal of basic microbiology
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abstract::The depolymerization of humic acids (HAs) obtained from low-rank coal (lignite) to fulvic acids (FAs) was investigated in a cell-free system (in vitro) using manganese peroxidase (MnP) from the white-rot fungus Clitocybula dusenii b11. MnP was produced in surface cultures of C. dusenii which were induced with manganes...
journal_title:Journal of basic microbiology
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abstract::Proteus mirabilis (P. mirabilis) is one of Gram-negative pathogens encountered in clinical specimens. A clinical isolate (TRP41) of P. mirabilis was isolated from a Turkish patient in Turkey. The isolate was identified using the API 32GN system and 16S rRNA gene sequencing and it was found resistant to ampicillin/sulb...
journal_title:Journal of basic microbiology
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abstract::Cassava wastes--the peel and the root fibre were taken through various pretreatment procedures before being subjected to solid state fermentation with Trichoderma harzianum. Most of the pretreatment processes increased the cellulose and hemicellulose content of the cassava peel and fibre by as high as 155% while sulfu...
journal_title:Journal of basic microbiology
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abstract::Epiphytic microbes are common inhabitants of freshwater submerged macrophytes, which play an important role in aquatic ecosystems. An important precondition for studying the epiphytic microbes is having an effective method of separating the attached microbes from the host macrophytes. We developed an ultrasound-based ...
journal_title:Journal of basic microbiology
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abstract::The acetic acid bacteria have mainly relevance for bacterial cellulose production and fermented bio-products manufacture. The purpose of this study was to identify temperate bacteriophages in a cellulose-producing bacterial strain Komagataeibacter intermedius IMBG180. Prophages from K. intermedius IMBG180 were induced...
journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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abstract::Mn-oxidizing potential of two metal-tolerant bacterial strains - Halomonas meridiana and Marinobacter algicola isolated from the South West Indian Ridge waters were compared at varying concentrations of Mn (II), i.e., 1, 10, and 100 μmol and mmol L-1 . Accompanying changes in their morphology and metabolism were also ...
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abstract::The aim of this study was to identify genes involved in biofilm development in the probiotic lactic acid bacterium Lactobacillus plantarum. The ability of L. plantarum LM3 and of some derivative mutant strains to form biofilm has been investigated. Biofilm microtitre plate assays showed that L. plantarum LM3-2, carryi...
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abstract::Mixed bacterial cultures are increasingly being used in the production of polyhydroxyalkanoates (PHAs), as they have the potential to be more cost effective than axenic pure cultures. The purpose of this study was to use pure cultures in combination to identify their potential of PHA production. In this work we used v...
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abstract::Ammonia oxidation, mainly driven by ammonia-oxidizing archaea (AOA) and bacteria (AOB), plays an important role in determining the rate of nitrification in riparian zones. However, the underlying factors driving the distribution and activity of AOA and AOB in riparian zones, especially in the rhizosphere of Phragmites...
journal_title:Journal of basic microbiology
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abstract::Efflux-mediated resistance has been recognized as an important contributor of antibiotic resistance in bacteria, especially in methicillin-resistant Staphylococcus aureus (MRSA) isolates. This study was carried out to detect and analyze efflux genes (norA and mdeA) and active efflux activity in a collection of Malaysi...
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abstract::The ability of four strains of Aspergillus parasiticus (IMI 301,001) and two strains of Aspergillus flavus (IMI 300,998) to produce aflatoxins on Nigerian groundnuts under varying conditions of temperature was studied. While all the A. parasiticus strains produced the four major aflatoxins (B1, B2, G1, G2), only aflat...
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abstract::107 species and 8 species varieties belonging to 44 genera were collected from combine harvester wheat and sorghum dusts (35 genera and 91 species + 4 varieties) and from the atmosphere of their hay sites (26 genera and 69 species + 4 varieties) on glucose- and cellulose-Czapek's Dox agar at 28 degrees C and 45 degree...
journal_title:Journal of basic microbiology
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abstract::The steroid 1-dehydrogenase of Rhodococcus erythropolis IMET 7030, an active steroid-transforming strain, was localized by immunogold labelling both in cells induced with 17-alpha-methyl-testosterone and in noninduced cells. The labelling intensity was much higher in induced cells than in noninduced cells, indicating ...
journal_title:Journal of basic microbiology
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