Improved cadmium resistance and removal capacity in Pichia kudriavzevii A16 by sucrose preincubation.

Abstract:

:Removal of heavy metals from food material by growing micro-organisms is limited by the toxicity to cells. In this study, different preincubation treatments were investigated to analyze their effects on cadmium resistance and removal ability of Pichia kudriavzevii A16 and Saccharomyces cerevisiae CICC1211. Sucrose preincubation improved the cadmium resistance of both yeast cells and increased the cadmium-removal rate of P. kudriavzevii A16. An evident decrease of intracellular and cell-surface cadmium accumulation was observed after sucrose preincubation, which may be the primary reason responsible for the improved cadmium resistance. Flow cytometry assay showed that sucrose significantly reduced the production of reactive oxygen species (ROS) and cell death rate of both yeasts under cadmium compared with those normally cultured cells. Under cadmium stress, the content of both protein carbonyls and malonyldialdehyde were also reduced by the addition of sucrose, the results were in accordance with the tendency of ROS, exhibiting a defending function of sucrose. Osmotic regulators as proline and trehalose were increased by sucrose preincubation in P. kudriavzevii A16 in the presence of cadmium. The results suggested that sucrose preincubation could be applied to improve cadmium resistance and removal rate of yeasts.

journal_name

J Basic Microbiol

authors

Zhang D,Ma N,Guo Z,Chen P,Ma R,Sun X,Wang D,Wang J,Xu Y

doi

10.1002/jobm.201900272

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

867-878

issue

9

eissn

0233-111X

issn

1521-4028

journal_volume

59

pub_type

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