Mutations and environmental factors affecting regulation of riboflavin synthesis and iron assimilation also cause oxidative stress in the yeast Pichia guilliermondii.

Abstract:

:Iron deficiency causes oversynthesis of riboflavin in several yeast species, known as flavinogenic yeasts. However, the mechanisms of such regulation are not known. We found that mutations causing riboflavin overproduction and iron hyperaccumulation (rib80, rib81 and hit1), as well as cobalt excess or iron deficiency all provoke oxidative stress in the Pichia guilliermondii yeast. Iron content in the cells, production both of riboflavin and malondialdehyde by P. guilliermondii wild type and hit1 mutant strains depend on a type of carbon source used in cultivation media. The data suggest that the regulation of riboflavin biosynthesis and iron assimilation in P. guilliermondii are linked with cellular oxidative state.

journal_name

J Basic Microbiol

authors

Boretsky YR,Protchenko OV,Prokopiv TM,Mukalov IO,Fedorovych DV,Sibirny AA

doi

10.1002/jobm.200610279

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

371-7

issue

5

eissn

0233-111X

issn

1521-4028

journal_volume

47

pub_type

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