Abstract:
:The present study describes the biosynthesis of proteases by a locally isolated mould culture of Rhizopus oligosporus IHS13 in a low-cost medium by solid-state fermentation technique. The fermentation was carried out in a low-cost medium such as sunflower meal, wheat bran and rice bran. Sunflower meal and wheat bran in a ratio of 1:1 and moistened with distilled water was found to be the best substrate for protease synthesis. All the three substrates are very cheap agricultural by-products found in Pakistan. The production of proteases in sunflower meal and rice bran was also investigated but the results were unsatisfactory. Different cultural conditions such as rate of fermentation, effect of incubation temperature, effect of pH and depth of the fermentation medium were also optimized. The maximum enzyme synthesis was found after 72 h of fermentation at a temperature of 30 degrees C. The optimum pH and depth of the medium for protease synthesis were found to be 5.0 and 20 mm respectively. The maximum enzyme biosynthesis found during the course of present studies was 7.0 U ml(-1).
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Haq IU,Mukhtar Hdoi
10.1002/jobm.200410393subject
Has Abstractpub_date
2004-01-01 00:00:00pages
280-7issue
4eissn
0233-111Xissn
1521-4028journal_volume
44pub_type
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