Isolation and molecular characterization of Shewanella sp. G5, a producer of cold-active beta-D-glucosidases.

Abstract:

:beta -Glucosidase is a highly desired glycosidase, especially for hydrolysis of glycoconjugated precursors in musts and wines for the release of active aromatic compounds. A Shewanella sp. G5 strain was isolated from the intestinal content of benthonic organism (Munida subrrugosa) from different coastal areas of the Beagle Channel, Tierra del Fuego (Argentina). This marine bacterium was able to grow at a temperature range between 4 to 20 degrees C using different beta-glycoside substrates, such as cellobiose, as carbon source. In this work, the Shewanella sp. G5 strain exhibited high beta-glucosidase activity on plate at low temperature (4 and 20 degrees C). Two genes encoding different cold-active beta-glucosidases were amplified and sequenced and the nucleotide sequences were submitted to the GenBank. 16S rDNA and gyrB gene sequences were used for the molecular characterization of Shewanella sp. G5.

journal_name

J Basic Microbiol

authors

Cristóbal HA,Breccia JD,Abate CM

doi

10.1002/jobm.200700146

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

16-24

issue

1

eissn

0233-111X

issn

1521-4028

journal_volume

48

pub_type

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