Abstract:
:Propidium iodide (PI) uptake and salt tolerance assays were used to investigate the mechanism of antibacterial action of an extract of the leaves of Eremophila duttonii, a traditional Australian medicinal plant previously shown to have potent bactericidal activity against Gram positive bacteria. The extract compromised the integrity of the cytoplasmic membrane of Staphylococcus aureus , leading to increased membrane permeability (indicated by uptake of PI) and a decrease in ability to exclude NaCl. The bactericidal action of the E. duttonii extract was concluded to be due to its membrane-active properties. The effect of contaminants on the efficacy of this extract and other medicinal plant extracts was also investigated. Organic contaminants (bakers' yeast and skim milk powder) decreased the efficacy of all extracts investigated, while hard water had no effect. Greater understanding of the biocidal properties of the plant extracts investigated may determine if they have medical, industrial or environmental applications. ((c) 2005 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Tomlinson S,Palombo EAdoi
10.1002/jobm.200410534subject
Has Abstractpub_date
2005-01-01 00:00:00pages
363-70issue
5eissn
0233-111Xissn
1521-4028journal_volume
45pub_type
杂志文章abstract::The long residual action and toxicity of the chemical insecticides have brought about serious environmental problems such as the emergence and spread of insecticide resistance in many species of vectors, mammalian toxicity, and accumulation of pesticide residues in the food chain. All these problems have highlighted t...
journal_title:Journal of basic microbiology
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abstract::This work reports the production of a novel serine protease enzyme (P. dig-protease) from the fungus Penicillium digitatum. The protease was purified from the culture supernatant to homogeneity using ammonium sulfate precipitation, Sephadex G-150 gel filtration and carboxymethyl-sepharose ion exchange chromatography w...
journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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abstract::Andisol in farmland located in Hokkaido, Japan, is known to actively flux nitrous oxide (N(2)O) during the spring to summer seasons. Using a culturing system which mimics farm soils, nitrous oxide (N(2)O) emission potentials of the soils or soil microorganisms were investigated. A total of thirty-three soil samples fr...
journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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abstract::Intracellular proteolytic activity was detected in the haloalkaliphilic archaeon Natronococcus occultus during the stationary phase of cultures grown in complete medium and during carbon and nitrogen starvation. Puromycin prevented the occurrence of proteolytic activity in starved cells, suggesting that de novo synthe...
journal_title:Journal of basic microbiology
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abstract::The role of rock inhabiting bacteria in potassium (K) solubilization from feldspar and their application in crop nutrition through substitution of fertilizer K was explored through the isolation of 36 different bacteria from rocks of a major hill station at Ponmudi in Thiruvananthapuram, Kerala, India. A comprehensive...
journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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