Abstract:
:Quorum sensing is a cell-cell signaling mechanism based on cell density and that involves the production of hormone-like molecules called autoinducers (AI). One of the most studied AIs has been termed AI-2, and its biosynthesis requires the enzyme encoded by luxS. We have previously described for the first time that Bacteroides species can produce molecules with AI-2 activity. In this study, we focus on the detection of luxS and its activity as the AI-2 synthase in Bacteroides species. The strains Bacteroides fragilis B3b and Bacteroides vulgatus ATCC 8482 were selected based on a positive phenotype for AI-2 production and the presence of a putative luxS in the genome, respectively. In order to identify the luxS gene, cloning and heterologous expression strategies were utilized. We demonstrate that both strains contain functional luxS orthologs that can complement AI-2 production in Escherichia coli.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Peixoto RJ,Miranda KR,Ferreira EO,de Paula GR,Rocha ER,Lobo LA,Domingues RMdoi
10.1002/jobm.201300311subject
Has Abstractpub_date
2014-07-01 00:00:00pages
644-9issue
7eissn
0233-111Xissn
1521-4028journal_volume
54pub_type
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