Purification and biochemical characterization of a novel protease from Penicillium digitatum - Use in bioactive peptides production.

abstract：:The objectives of the present study were to purify and assess the killer toxin effect produced by Aureobasidium pullulans under casual agents of green mold (Penicillum digitatum) and sour rot (Geotrichum citri-aurantii). Initially, different methods of protein precipitation were tested. The proteolytic activity and th...

journal_title：Journal of basic microbiology

authors： Moura VS,Pollettini FL,Ferraz LP,Mazzi MV,Kupper KC

更新日期：2020-12-29 00:00:00

abstract：:Hyoscyamus muticus L. (Egyptian henbane) is one of the desert medicinal plants of family Solanaceae. The plant produces pharmaceutically important compounds (tropane alkaloids) as secondary metabolites. In the present study, we describe mycoflora of H. muticus grown in four different locations in Egyptian southern des...

journal_title：Journal of basic microbiology

authors： El-Zayat SA,Nassar MS,El-Hissy FT,Abdel-Motaal FF,Ito S

更新日期：2008-04-01 00:00:00

abstract：:The possibility of associating starch degradation with bacterial beta-glucuronidase expression was examined. We proved that starving, in starch medium, amylase-negative Escherichia coli (M94) which has constitutive beta-glucuronidase greatly reduces (p < 0.01) its background activity, but the addition of both cell-fre...

journal_title：Journal of basic microbiology

authors： Cenci G,Caldini G,Trotta F

更新日期：2000-01-01 00:00:00

abstract：:Spore (akinete) formation in the heterocystous and branched filamentous cyanobacterium Fischerella muscicola involves a significant increase in cell size and formation of several endospores in each of the cells. In present study, the physico-chemical factors (pH, light sources, nutrient deficiency, nitrogen sources, c...

journal_title：Journal of basic microbiology

authors： Mishra BN,Kaushik MS,Abraham G,Singh PK

更新日期：2018-08-01 00:00:00

abstract：:Studies were carried out to screen twelve strains of Lentinus edodes for their efficiency to grow on toxic agro-industrial residues of coffee industry in solid state cultivation (SSC). Based on best mycelial growth (7.57 mm/day) and biomass production (48.78 mg/plate in 12 days at 24 degrees C) in coffee husk extract ...

journal_title：Journal of basic microbiology

authors： Leifa F,Pandey A,Soccol CR

更新日期：2000-01-01 00:00:00

abstract：:The diversity of cellulolytic bacteria from the rice-pulse system can be sourced for identification of efficient cellulose decomposing microbial strains. In the present study, the abundance, structural diversity, and cellulolytic potential of the culturable bacterial community were studied in 5-year old rice-pulse sys...

journal_title：Journal of basic microbiology

authors： Dash PK,Bhattacharyya P,Shahid M,Roy PS,Padhy SR,Swain CK,Kumar U,Kumar A,Gautam P,Lal B,Panneerselvam P,Nayak AK

更新日期：2019-10-01 00:00:00

abstract：:A subtraction library of group B streptococcus (GBS) strain O9OR with GAS chromosomal DNA (strain SF370) was constructed and more than 100 plasmid clones sequenced. DNA sequences of the plasmid inserts were analyzed using the BLAST gene search. Most inserts had little or no homology to GAS chromosomal DNA and 26 clone...

journal_title：Journal of basic microbiology

authors： Suvorov AN,Ferretti JJ

更新日期：2004-01-01 00:00:00

abstract：:The presence of the ica loci and adhesins genes in clinical Staphylococcus aureus strains were considered important factors of virulence. In this study, 46 strains of Staphylococcus aureus were isolated from auricular infection, and were investigated for slime production using Congo Red Agar method (CRA). In order to ...

journal_title：Journal of basic microbiology

authors： Zmantar T,Chaieb K,Makni H,Miladi H,Abdallah FB,Mahdouani K,Bakhrouf A

更新日期：2008-08-01 00:00:00

abstract：:The effects of cold-shock on Neurospora crassa was here investigated. In cold-shock treated hyphae (6 h at 4 degrees C) the branching pattern was changed and dichotomy as well as a fan-like-pattern structure were temporarily detected at the tips. When exogenous Ca(2+) (500 mM) was added to the hyphae, these morphologi...

journal_title：Journal of basic microbiology

authors： Kawano CY,Said S

更新日期：2002-01-01 00:00:00

abstract：:An extracellular lipase isolated from Bacillus megaterium AKG-1 had an optimum activity at 55 degrees C/pH 7.0. It retained 100% activity at 50 degrees C for 30 min with a half life of 30 min at 70 degrees C. A 20-70% increase in lipase activity was observed in presence of acetone (20% v/v), DMSO (20% v/v) and isoprop...

journal_title：Journal of basic microbiology

authors： Sekhon A,Dahiya N,Tiwari RP,Hoondal GS

更新日期：2005-01-01 00:00:00

abstract：:Total cellular proteins and fatty acid composition profiles of mid-log phase cells of Salmonella enterica serovar Typhimurium grown at 8, 25, 37 or 42 °C were separated by 2D-PAGE and FAME analysis. Growth temperature associated protein expression can be grouped into 3 thermal classes which include proteins whose expr...

journal_title：Journal of basic microbiology

authors： Balamurugan S,Dugan ME

更新日期：2010-12-01 00:00:00

abstract：:Ribosomal efficiency (RE) estimates provide a quantitative descriptor of intrinsic growth rate of cell populations using readily-obtainable experimental data. In Aspergillus nidulans chemostat cultures, RE increased linearly with growth rate over the range 25-60% of maximum growth rate (mu max), consistent with increa...

journal_title：Journal of basic microbiology

authors： Bushell ME,Bull AT

更新日期：1999-01-01 00:00:00

abstract：:Protein turnover affects protein abundance and phenotypes. Comprehensive investigation of protein turnover dynamics has the potential to provide substantial information about gene expression. Here we report a large-scale protein turnover study in Salmonella Typhimurium during infection by quantitative proteomics. Muri...

journal_title：Journal of basic microbiology

authors： Wang Z,Han QQ,Zhou MT,Chen X,Guo L

更新日期：2016-07-01 00:00:00

abstract：:Rock phosphate effect on English mint (Mentha piperita L.) grown on steamed perlite:vermiculite (1:1, v:v) substrate, with and without rock phosphate, was evaluated in greenhouse experiments. Five treatments were carried out by inoculation with an arbuscular mycorrhizal fungus Glomus mosseae and a phosphorus solubiliz...

journal_title：Journal of basic microbiology

authors： Cabello M,Irrazabal G,Bucsinszky AM,Saparrat M,Schalamuk S

更新日期：2005-01-01 00:00:00

abstract：:The present study demonstrates cloning, expression, and characterization of hyperthermostable L-asparaginase from Thermococcus kodakarensis KOD1 in Escherichia coli BLR(DE3). The recombinant 6× His-tagged protein L-asparaginase from T. kodakarensis (TkAsn), was purified to homogeneity by heat treatment followed by aff...

journal_title：Journal of basic microbiology

authors： Hong SJ,Lee YH,Khan AR,Ullah I,Lee C,Park CK,Shin JH

更新日期：2014-06-01 00:00:00

abstract：:In the past years, research on the flagellate unicellular alga Chlamydomonas reinhardtii has entered a new era based on the availability of its complete genome. Since this green alga can be grown relatively easy in a short time-range, sufficient biological material is available to efficiently establish biochemical pur...

journal_title：Journal of basic microbiology

authors： Wagner V,Boesger J,Mittag M

更新日期：2009-02-01 00:00:00

abstract：:Cold acclimation is an important process to increase freezing tolerance for over-winter survival in many organisms. The apoplastic area is very important in cold acclimation. Two-dimensional electrophoresis was used to identify apoplastic proteins involved in the cold acclimation process of the filamentous fungus Magn...

journal_title：Journal of basic microbiology

authors： Li J,Jia B,Liang X,Liu J,Wang Y,Liang X,Yan H,Wang Y,Zhang S

更新日期：2014-04-01 00:00:00

abstract：:The activity to metabolize the polycyclic aromatic hydrocarbons (PAH) phenanthrene, anthracene, pyrene, fluorene and fluoranthene by Trametes versicolor, Pleurotus ostreatus (white rot fungi), Laetiporus sulphureus, Daedaela quercina, Flamulina velutipes (brown rot fungi), Marasmiellus sp. (litter decaying fungus) and...

journal_title：Journal of basic microbiology

authors： Sack U,Günther T

更新日期：1993-01-01 00:00:00

abstract：:The API 50CH and API ZYM systems fulfil an important role in the polyphasic taxonomic identification of lactobacilli. When the API 50CH fermentation profile of the quality control Lactobacillus casei var. alactosus (Lb. paracasei subsp. paracasei) strain NCFB 206 was determined at 37 degrees C, it was found to differ ...

journal_title：Journal of basic microbiology

authors： Charteris WP,Kelly PM,Morelli L,Collins JK

更新日期：2001-01-01 00:00:00

abstract：:A 2-haloacid dehalogenase-producing bacterium, designated DEH99, was isolated from the marine sponge Hymeniacidon perlevis using a modified enrichment medium and a pH indicator method. DEH99 could degrade only half of the racemic mixture 2-chloropropionic acid (2-CPA) in the medium. The dehalogenase of DEH99 was furth...

journal_title：Journal of basic microbiology

authors： Huang J,Xin Y,Zhang W

更新日期：2011-06-01 00:00:00

abstract：:In vitro effect of salivary gland extract from fed Ixodes ricinus, the competent vector of Lyme borreliosis in Europe, on the growth of Borrelia burgdorferi sensu lato (B. garinii, B. afzelii and B. burgdorferi sensu stricto) was examined in BSK-H medium. Motility rate, concentration of motile spirochetes and their mo...

journal_title：Journal of basic microbiology

authors： Rudolf I,Sikutová S,Kopecký J,Hubálek Z

更新日期：2010-06-01 00:00:00

abstract：:Bacteria capable of methylating Hg2+ have been isolated from sediment, water, soil and the gastrointestinal tract of humans. However, very little is known about the physiology and genetics of the mechanisms controlling Hg2+ methylation. Mercury methylation can be either chromosomal or plasmid-encoded in bacteria. In a...

journal_title：Journal of basic microbiology

authors： Trevors JT

更新日期：1986-01-01 00:00:00

abstract：:Studies on Acidithiobacillus ferrooxidans accepting electrons from Fe(II) have previously focused on cytochrome c. However, we have discovered that, besides cytochrome c, type IV pili (Tfp) can transfer electrons. Here, we report conduction by Tfp of A. ferrooxidans analyzed with a conducting-probe atomic force micros...

journal_title：Journal of basic microbiology

authors： Li Y,Li H

更新日期：2014-03-01 00:00:00

abstract：:Trichoderma atroviride (T-15603.1) is a promising fungal agent for biological control of wood decay fungi in urban tree wounds. The aim of this work was to determine the combined effects of water activity (a(w), 0.998-0.892), temperature (10-30 °C) and pH (3-7) on the development of conidia on low-nutrient agar (LNA)....

journal_title：Journal of basic microbiology

authors： Schubert M,Mourad S,Schwarze FW

更新日期：2010-12-01 00:00:00

abstract：:This study focuses on analyzing the protein expression pattern of intracellular proteins when Pseudomonas mendocina SMSKVR-3 exposed to 300 mM of arsenate to find out the proteins that are overexpressed or exclusively expressed in response to arsenate. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis anal...

journal_title：Journal of basic microbiology

authors： Mishra S,Verma SK

更新日期：2021-01-14 00:00:00

abstract：:Transposons are a group of mobile genetic elements that are defined as a DNA sequence. Transposons can jump into different places of the genome; for this reason, they are called jumping genes. However, some transposons are always kept at the insertion site in the genome. Most transposons are inactivated and as a resul...

journal_title：Journal of basic microbiology

authors： Babakhani S,Oloomi M

更新日期：2018-11-01 00:00:00

abstract：:Spontaneously occurring thallium-resistant (Tl+ -R) mutant of diazotrophic, heterocystous cyanobacterium Nostoc muscorum is described in which Tl+ -R phenotype is the cause of defective salinity-inducible K+ uptake activity and proline uptake activity. The results indicate that mutant strain is more sensitive to salin...

journal_title：Journal of basic microbiology

authors： Bhargava S

更新日期：2005-01-01 00:00:00

abstract：:Trichoderma spp. are versatile beneficial fungi which can stimulate growth and plant resistance to biotic and abiotic stresses. In this study, the potential of Trichoderma isolate in promoting the cucumber growth under salt stress and its possible mechanisms were investigated. Strain Q1 was isolated from the rhizosphe...

journal_title：Journal of basic microbiology

authors： Qi W,Zhao L

更新日期：2013-04-01 00:00:00

abstract：:Leaf-cutting ants modify the properties of the soil adjacent to their nests. Here, we examined whether such an ant-altered environment impacts the belowground fungal communities. Fungal diversity and community structure of soil from the fungus garden chambers of Atta sexdens rubropilosa and Atta bisphaerica, two wides...

journal_title：Journal of basic microbiology

authors： Rodrigues A,Passarini MR,Ferro M,Nagamoto NS,Forti LC,Bacci M Jr,Sette LD,Pagnocca FC

更新日期：2014-11-01 00:00:00

abstract：:Lyme disease, caused by Borrelia burgdorferi, is a focally endemic tick-transmitted zoonotic infection. In this study, the major factors underlying synonymous codon-related amino acid usage in the B. burgdorferi genome and bias in synonymous codon usage of the translation initiation region of coding sequences were ana...

journal_title：Journal of basic microbiology

authors： Ma XX,Ma P,Chang QY,Liu ZB,Zhang D,Zhou XK,Ma ZR,Cao X

更新日期：2018-05-01 00:00:00