Sporulation at minimum specific growth rate in Aspergillus nidulans chemostat culture predicted using protein synthesis efficiency estimations.

Abstract:

:Ribosomal efficiency (RE) estimates provide a quantitative descriptor of intrinsic growth rate of cell populations using readily-obtainable experimental data. In Aspergillus nidulans chemostat cultures, RE increased linearly with growth rate over the range 25-60% of maximum growth rate (mu max), consistent with increasing ribosomal usage with increased growth rate. Above 60%, RE did not increase significantly, suggesting that all ribosomes were functional at 60% of mu max, further increases in growth rate, presumably resulting from increased polypeptide chain elongation rate. Extrapolating the linear part of the RE/growth rate curve predicted zero RE at a growth rate of 0.04 h-1. Chemostat steady state cultures at 0.04 h-1 contained spores (conidia), apparently undergoing a continuous sporulation/germination cycle. We propose that the RE estimates provide a means of predicting the value of minimum specific growth rate (mu min) below which net growth cannot take place.

journal_name

J Basic Microbiol

authors

Bushell ME,Bull AT

doi

10.1002/(sici)1521-4028(199912)39:5/6<293::aid-job

subject

Has Abstract

pub_date

1999-01-01 00:00:00

pages

293-8

issue

5-6

eissn

0233-111X

issn

1521-4028

pii

10.1002/(SICI)1521-4028(199912)39:5/6<293::AID-JOB

journal_volume

39

pub_type

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