Alcohol oxidation by Acinetobacter calcoaceticus EB 104--a n-alkane-utilizing and cytochrome P-450-producing strain.

Abstract:

:A soluble, NADP+-dependent alcohol dehydrogenase (ADH) with a pH optimum at 8.7 was found in A. calcoaceticus EB 104 after growth on different carbon sources. n-Alkanols with short and medium chain length were employed as test substrates. The Km values decreased with increasing chain length. The Vmax values remained nearly unchanged. The activities determined were independent of the carbon source. Furthermore, a n-alkanol-dependent reduction of DCPIP was measured in membrane fractions of cells grown on different carbon sources. The optimum pH for this reaction was at 7.5. Further proof for the presence of a pyridine nucleotide-independent ADH was derived from the oxidation of 14C-decanol in the absence of NADP+ or NAD+.

journal_name

J Basic Microbiol

authors

Jirausch M,Asperger O,Kleber HP

doi

10.1002/jobm.3620260611

subject

Has Abstract

pub_date

1986-01-01 00:00:00

pages

351-7

issue

6

eissn

0233-111X

issn

1521-4028

journal_volume

26

pub_type

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