Abstract:
:A soluble, NADP+-dependent alcohol dehydrogenase (ADH) with a pH optimum at 8.7 was found in A. calcoaceticus EB 104 after growth on different carbon sources. n-Alkanols with short and medium chain length were employed as test substrates. The Km values decreased with increasing chain length. The Vmax values remained nearly unchanged. The activities determined were independent of the carbon source. Furthermore, a n-alkanol-dependent reduction of DCPIP was measured in membrane fractions of cells grown on different carbon sources. The optimum pH for this reaction was at 7.5. Further proof for the presence of a pyridine nucleotide-independent ADH was derived from the oxidation of 14C-decanol in the absence of NADP+ or NAD+.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Jirausch M,Asperger O,Kleber HPdoi
10.1002/jobm.3620260611subject
Has Abstractpub_date
1986-01-01 00:00:00pages
351-7issue
6eissn
0233-111Xissn
1521-4028journal_volume
26pub_type
杂志文章abstract::The fungal population of Aswan High Dam Lake showed marked vertical variations during the period of study which extended from July 1985 to December 1986. High fungal counts were observed at the surface water which were mainly due to the high counts of Aspergillus fumigatus and A. terreus. Going deeper the fungal popul...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620300311
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abstract::This study was conducted to determine what effects nanoparticles (NPs) like TiO2 , ZnO, and Ag may pose on natural attenuation processes of petroleum hydrocarbons in contaminated soils. The solid NPs used were identified using x-ray diffraction technique and their average size was certified as 18.2, 16.9, and 18.3 nm ...
journal_title:Journal of basic microbiology
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abstract::Sphingobium sp. SYK-6 can utilize lignin biphenyl compounds, such as 2,2'-dihydroxy-3,3'-dimethoxy-5,5'-dicarboxybiphenyl (DDVA). In the metabolism of DDVA, this microorganism exploits two O -demethylation systems with different substrate specificities, namely, a DDVA-specific oxygenative O -demethylation system and a...
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abstract::A comparative electron microscopic analysis of weakly beta-haemolytic spirochaetes related to human and animal intestinal spirochaetosis was done in order to search for the presence of inducible bacteriophages associated with these spirochaetes. Bacteriophages were detected at the electron microscope after experimenta...
journal_title:Journal of basic microbiology
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abstract::Removal of heavy metals from food material by growing micro-organisms is limited by the toxicity to cells. In this study, different preincubation treatments were investigated to analyze their effects on cadmium resistance and removal ability of Pichia kudriavzevii A16 and Saccharomyces cerevisiae CICC1211. Sucrose pre...
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abstract::Gordoniae are one of the most promising hydrocarbon-oxidizing actinobacteria. Here we present the genome sequence analysis of thermotolerant strain Gordonia sp. 1D isolated from oil-refinery soil. It is capable of alkane consumption and biosurfactant production at temperatures of up to 50°C. Gordonia sp. 1D demonstrat...
journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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abstract::The effect of continuous-wave ultrasound on the viability of Escherichia coli HB101 was assessed using a 20 kHz ultrasonic processor. A standardised cell suspension of fixed concentration was used to investigate the influence of different physical and environmental conditions on ultrasound susceptibility. Cell viabili...
journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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abstract::Gamma-hexachlorocyclohexane (gamma-HCH or lindane), one of the most commonly used pesticides, has been mainly used in agriculture; this pesticide is known to be highly toxic and persistent, causing serious water and soil contamination. The objective of the present work is to study the effect of low glucose concentrati...
journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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abstract::Statistically-based experimental designs were applied to optimize the fermentation for the production of laccase by Pleurotus florida NCIM 1243. Eleven components were screened for their significant effect on laccase production using Plackett-Burman factorial design. Glucose (carbon source), asparagine (nitrogen sourc...
journal_title:Journal of basic microbiology
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abstract::An alkaline serine protease produced by Bacillus megaterium RRM2 isolated from the red alga, Kappaphycus alvarezii (Doty) Doty ex Silva was studied for the first time and the same analyzed for the production of protease in the present study. Identification of the bacterium was done on the basis of both biochemical ana...
journal_title:Journal of basic microbiology
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abstract::This work reports the production of a novel serine protease enzyme (P. dig-protease) from the fungus Penicillium digitatum. The protease was purified from the culture supernatant to homogeneity using ammonium sulfate precipitation, Sephadex G-150 gel filtration and carboxymethyl-sepharose ion exchange chromatography w...
journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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abstract::Crude glucoamylase preparation from Rhizoctonia solani was used to saccharify raw and cooked starch. Various concentrations of potato starch and wheat flour from 10-40%, w/v were used for mashing but 30% was found to be the optimal and economical. The saccharified mash yielded 5.89%, v/v ethanol in a simultaneous sacc...
journal_title:Journal of basic microbiology
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abstract::A mosquitocidal soil isolate of Bacillus thuringiensis serovar fukuokaensis (H3ade) produced spherical parasporal inclusions measuring 0.6-0.7 microm in diameter. Inclusion matrix was homogeneous substance surrounded by a thick, highly electron-dense envelope 30-50 nm in thickness. The envelopes were associated with b...
journal_title:Journal of basic microbiology
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journal_title:Journal of basic microbiology
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abstract::There was a considerable change in the structural parameters of both the lignocellulosic substrates. This change was confirmed by infrared (IR) spectroscopy and X-ray diffraction data, which revealed the presence of phenolic groups in the peaks range 1310-1410 and 1200 cm-1 with large crystalline substrates. These wer...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620330104
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abstract::An extracellular cold-adapted alkaline lipase from the psychrotrophic Yersinia enterocolitica strain KM1 was purified 26-fold to homogeneity. The enzyme was active over a broad range spanning 0-60 °C with an optimum activity at 37 °C, and it was found to be alkaline-preferring with an optimum activity at pH 9.0. The m...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
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abstract::Pichia pastoris expression system was widely used for producing heterologous proteins. Some researches revealed that the increase in the copy number could improve the expression of foreign genes in P. pastoris. Hundreds or thousands of antibiotic-resistance recombinants need to be screened because the frequency of mul...
journal_title:Journal of basic microbiology
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abstract::Microorganisms such as bacteria and molds produce an enormous variety of volatile metabolites. To determine whether typical microbial volatile metabolites can be used as indicator compounds for the detection of hidden mold in indoor environments, we examined 14 typical indoor fungal strains for their growth rates and ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
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abstract::Intracellular aminopeptidase synthesized by a soil strain of Pseudomonas sp. was purified 323-fold using the following procedure: saturation with ammonium sulfate, separation by preparative electrophoresis, anion-exchange chromatography and gel filtration chromatography. Molecular weight of the enzyme determined accor...
journal_title:Journal of basic microbiology
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abstract::Surface cultures of Fusarium culmorum JP15 were found to respond to extracts of other fungi by enhanced production of orange-red fusarubin pigments and formation of aerial mycelium. Two inducers from strain Ulocladium sp. HKI 0226, the new (-)-terpestacin (1) and L-tenuazonic acid (2), were isolated. 1 inhibited syncy...
journal_title:Journal of basic microbiology
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abstract::The effects of cold-shock on Neurospora crassa was here investigated. In cold-shock treated hyphae (6 h at 4 degrees C) the branching pattern was changed and dichotomy as well as a fan-like-pattern structure were temporarily detected at the tips. When exogenous Ca(2+) (500 mM) was added to the hyphae, these morphologi...
journal_title:Journal of basic microbiology
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abstract::The close linkage between hisI and mutations of the cysII group in Pseudomonas aeruginosa was confirmed by mapping experiments with new mutants. All mutants of the cysII group were blocked in the cysteine biosynthesis before sulfite (this include the mutant cys-5605 which had previously been mapped by Mee and Lee, 196...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620260120
更新日期:1986-01-01 00:00:00