Abstract:
:This study was conducted to determine what effects nanoparticles (NPs) like TiO2 , ZnO, and Ag may pose on natural attenuation processes of petroleum hydrocarbons in contaminated soils. The solid NPs used were identified using x-ray diffraction technique and their average size was certified as 18.2, 16.9, and 18.3 nm for Ag-NPs, ZnO-NPs, and TiO2 -NPs, respectively. NPs in soil microcosms behave differently where it was dissolved as in case of Ag-NPs, partially dissolved as in ZnO-NPs or changed into other crystalline phase as in TiO2 -NPs. In this investigation, catabolic gene encoding catechol 2,3 dioxygenase (C23DO) was selected specifically as biomarker for monitoring hydrocarbon biodegradation potential by measuring its transcripts by RT-qPCR. TiO2 -NPs amended microcosms showed almost no change in C23DO expression profile or bacterial community which were dominated by Bacillus sp., Mycobacterium sp., Microbacterium sp., Clostridium sp., beside uncultured bacteria, including uncultured proteobacteria, Thauera sp. and Clostridia. XRD pattern suggested that TiO2 -NPs in microcosms were changed into other non-inhibitory crystalline phase, consequently, showing the maximum degradation profile for most low molecular weight oil fractions and partially for the high molecular weight ones. Increasing ZnO-NPs concentration in microcosms resulted in a reduction in the expression of C23DO with a concomitant slight deteriorative effect on bacterial populations ending up with elimination of Clostridium sp., Thauera sp., and uncultured proteobacteria. The oil-degradation efficiency was reduced compared to TiO2 -NPs amended microcosms. In microcosms, Ag-NPs were not detected in the crystalline form but were available in the ionic form that inhibited most bacterial populations and resulted in a limited degradation profile of oil, specifically the low molecular weight fractions. Ag-NPs amended microcosms showed a significant reduction (80%) in C23DO gene expression and a detrimental effect on bacterial populations including key players like Mycobacterium sp., Microbacterium sp., and Thauera sp. involved in the biodegradation of petroleum hydrocarbons.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
El-Sayed WS,Elbahloul Y,Saad ME,Hanafy AM,Hegazi AH,ElShafei GMS,Elbadry Mdoi
10.1002/jobm.201800186subject
Has Abstractpub_date
2019-02-01 00:00:00pages
166-180issue
2eissn
0233-111Xissn
1521-4028journal_volume
59pub_type
杂志文章abstract::A study was performed to develop a fermented milk beverage with the aim to increase the potential application of buffalo cheese whey and soymilk. A mixed substrate was prepared by selective combination, which contained buffalo cheese whey 35%, soymilk 30% and cow milk 35%. The substrate mixture was fermented by a mixe...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/(sici)1521-4028(199909)39:4<243::aid-jobm2
更新日期:1999-01-01 00:00:00
abstract::The role of rock inhabiting bacteria in potassium (K) solubilization from feldspar and their application in crop nutrition through substitution of fertilizer K was explored through the isolation of 36 different bacteria from rocks of a major hill station at Ponmudi in Thiruvananthapuram, Kerala, India. A comprehensive...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201500139
更新日期:2016-01-01 00:00:00
abstract::Mixed bacterial cultures are increasingly being used in the production of polyhydroxyalkanoates (PHAs), as they have the potential to be more cost effective than axenic pure cultures. The purpose of this study was to use pure cultures in combination to identify their potential of PHA production. In this work we used v...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201700276
更新日期:2018-03-01 00:00:00
abstract::Many ecosystems are currently co-contaminated with heavy metals such as cadmium (Cd(2+) ) and pesticides such as chlorpyrifos (CP) and γ-hexachlorocyclohexane (γ-HCH). A feasible approach to remediate the combined pollution of heavy metals and pesticides is the use of γ-HCH degrading bacteria endowed with CP hydrolysi...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201500559
更新日期:2016-07-01 00:00:00
abstract::Endophytes represent a hidden world within plants. Almost all plants that are studied harbor one or more endophytes, which help their host to survive against pathogens and changing adverse environmental conditions. Fungal and bacterial endophytes with distinct ecological niches show important biological activities and...
journal_title:Journal of basic microbiology
pub_type: 杂志文章,评审
doi:10.1002/jobm.201700275
更新日期:2017-10-01 00:00:00
abstract::In this study, diisodecyl phthalate (DIDP) was efficiently degraded by Bacillus sp. SB-007. The optimal conditions for DIDP (100 mg l(-1)) degradation by Bacillus sp. SB-007 in a mineral salts medium were found to be pH 7.0 at 30 degrees C, stirring at 200 rpm. The specific rate of DIDP degradation was found to be con...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200800297
更新日期:2009-09-01 00:00:00
abstract::The recA gene of Proteus mirabilis (recApm) has been cloned into the PstI site of the Bacillus promoter-probe plasmid pPL603. When present on this plasmid, the recApm1) gene is expressed in B. subtilis under the control of its own transcriptional and translational signals. It is concluded that the high AT-content of t...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620260202
更新日期:1986-01-01 00:00:00
abstract::The prokaryotic diversity in two brackish lakes (Sayram Lake and Chaiwopu Lake) was investigated by constructing bacterial and archaeal clone libraries of 16S rRNA genes. Bacterial clones from Sayram Lake were classified into six phyla (Proteobacteria, Verrucomicrobia, Bacteroidetes, Planctomycetes, Acidobacteria, Act...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201300187
更新日期:2014-06-01 00:00:00
abstract::In most filamentous basidiomycetes, clamp cells are found at the septa of dikaryotic mycelia. Clamp cell formation starts at hyphal tip cells with the development of a lateral bulge at a position slightly apical to the future septum. Relative to the growth direction of the hypha, the protrusion expands backwards into ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200310361
更新日期:2004-01-01 00:00:00
abstract::The effects of soil microbial properties and physiographical factors on safflower distributions in the main safflower plantations of Xinjiang province in China were studied. This study may help determine the basis of the environmental factors for evaluating the geoherbalism of this medicinal plant. The soil microbial ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201900626
更新日期:2020-06-01 00:00:00
abstract::Sixteen Bacillus thuringiensis (Bt) strains were screened for their anti-insect, antibacterial and antifungal determinants by phenotypic tests and PCR targeting major insecticidal proteins and complements, chitinases, lactonases, beta-1,3-glucanases and zwittermicinA. Six strains had genes of at least two major insect...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200800182
更新日期:2009-06-01 00:00:00
abstract::The objective was to screen for and isolate a novel enzyme with the specific activity of a Δ6-fatty acid desaturase from Rhizopus oryzae. In this study, R. oryzae was identified as a novel fungal species that produces large amounts of γ-linolenic acid. A full-length cDNA, designated here as RoD6D, with high homology t...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201200189
更新日期:2013-09-01 00:00:00
abstract::Penicillium chrysogenum secretes a low molecular weight, cationic and cysteine-rich protein (PAF). It has growth inhibitory activity against the model organism Aspergillus nidulans and numerous zoo- and phytopathogenic fungi but shows only minimal conditional antifungal activity against the producing organism itself. ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201000321
更新日期:2011-06-01 00:00:00
abstract::The outer membrane protein (OMP) profiles of 23 blood isolates of Acinetobacter baumannii representing all the different antimicrobial susceptibility patterns observed during a 3-year period in a Spanish hospital were studied. OMPs extracted from envelopes of sonicated cells after solubilisation with 2% of N-lauryl-sa...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200390022
更新日期:2003-01-01 00:00:00
abstract::Spore (akinete) formation in the heterocystous and branched filamentous cyanobacterium Fischerella muscicola involves a significant increase in cell size and formation of several endospores in each of the cells. In present study, the physico-chemical factors (pH, light sources, nutrient deficiency, nitrogen sources, c...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201800161
更新日期:2018-08-01 00:00:00
abstract::Tricholoma matsutake (S. Ito et Imai) is an ectomycorrhizal basidiomycete associated with Pinaceae and Fagaceae trees in the Northern Hemisphere. It is still unknown whether the symbiotic relationship with this ectomycorrhiza could affect the host plant's endophytic microbial community. In this study, we used high thr...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201700506
更新日期:2018-03-01 00:00:00
abstract::Optimization of indigo production process from indole using a newly isolated phenol-degrading bacterial strain was performed by Plackett-Burman design and response surface methodology. The strain designated as QM was identified as Pseudomonas sp. according to 16S rDNA analysis. Spectrum analysis of indole biotransform...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201100516
更新日期:2012-12-01 00:00:00
abstract::The key to improving plant productivity is successful bacterial-plant interaction in the rhizosphere that can be maintained in the environment. The results presented here confirm Azospirillum brasilense strain SM as a competent plant growth promoting bacterium over mid- and long-term associations with sorghum. This st...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201100038
更新日期:2012-04-01 00:00:00
abstract::Chiral intermediates have wide application and high demand in pharmaceutical, agricultural, and other biotechnological industries for the preparation of bulk drug substances or fine chemicals. (S)-1-(1-napthyl) ethanol is an important synthetic intermediate of mevinic acid analog and a potential inhibitor of 3-hydroxy...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201700207
更新日期:2017-09-01 00:00:00
abstract::Hydrophobins are a family of small, moderately hydrophobic proteins with eight cysteine residues arranged in a conserved pattern. A full-length cDNA, designated Po.hyd, corresponding to a hydrophobin gene of Pleurotus ostreatus was obtained in our previous work. The Po.hyd gene contains a 333 bp open reading frame (OR...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200710317
更新日期:2007-08-01 00:00:00
abstract::A gene encoding an organic solvent-stable protease was amplified from Pseudomonas aeruginosa strain K by polymerase chain reaction using consensus primers based on multiple sequence alignment of alkaline and metalloprotease genes from Pseudomonas species. The gene, which consisted of 1440 bp nucleotides and deduced 47...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200900133
更新日期:2010-04-01 00:00:00
abstract::Yarrowia lipolytica yeast was grown batchwise on n-hexadecane as the carbon and energy source. Two cerebroside species were quantitatively isolated from sphingolipid fractions of total lipids by a combination of column chromatography and preparative high-performance thin-layer chromatography. The cerebroside content a...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200310337
更新日期:2004-01-01 00:00:00
abstract::In the present study, we attempted to elucidate if the harmful phytopathogenic bacteria of Pectobacterium genus (P. atrosepticum) possess the enzymes for oxidation of phenolic compounds. Polyphenol oxidase (laccase) activity was revealed in P. atrosepticum cell lysates. Using bioinformatic analysis, an ORF encoding a ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201700413
更新日期:2017-12-01 00:00:00
abstract::Cassava wastes--the peel and the root fibre were taken through various pretreatment procedures before being subjected to solid state fermentation with Trichoderma harzianum. Most of the pretreatment processes increased the cellulose and hemicellulose content of the cassava peel and fibre by as high as 155% while sulfu...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620360607
更新日期:1996-01-01 00:00:00
abstract::A culture was isolated from an industrial mercuric salt-contaminated soil, which could tolerate Cd, Co, Zn, Cr, and Hg up to 190, 525, 350, 935, and 370 μM, respectively. The isolate was identified as Lysinibacillus sphaericus by 16S rRNA gene sequencing. It bioaccumulated Cd, Co, and Zn, and reductively detoxified Cr...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201300308
更新日期:2015-03-01 00:00:00
abstract::A comparative electron microscopic analysis of weakly beta-haemolytic spirochaetes related to human and animal intestinal spirochaetosis was done in order to search for the presence of inducible bacteriophages associated with these spirochaetes. Bacteriophages were detected at the electron microscope after experimenta...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::This work reports the production of a novel serine protease enzyme (P. dig-protease) from the fungus Penicillium digitatum. The protease was purified from the culture supernatant to homogeneity using ammonium sulfate precipitation, Sephadex G-150 gel filtration and carboxymethyl-sepharose ion exchange chromatography w...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201400179
更新日期:2014-07-01 00:00:00
abstract::An Indian indigenous, Loktak Lake soil isolate Streptomyces violascens MT7 was assessed for its biocontrol potential both in vitro and in vivo against toxigenic fruit-rotting fungi. Strain MT7 exhibited broad-spectrum antifungal activity against various pathogenic postharvest fungi of citrus and papaya. In shake-flask...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201500323
更新日期:2015-12-01 00:00:00
abstract::A comparative study of the wild type A. nidulans and a mutant strain aco-T69 (lacking conidia and cleistothecia) revealed the better production of beta-1,4-glucosidase in the former. The relative distribution of the enzyme levels in various morphological structures viz. somatic hyphae, spores and cleistothecia also sh...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620300513
更新日期:1990-01-01 00:00:00
abstract::Histone-like nucleoid-structuring protein (H-NS) and integration host factor (IHF) are major nucleoid-associated proteins, and DnaA, a replication initiator, may also be related with nucleoid compaction. It has been shown that protein-dependent DNA compaction is related with many aspects of bacterial physiology, inclu...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201900497
更新日期:2020-02-01 00:00:00