Characterization of a Pleurotus ostreatus fruiting body-specific hydrophobin gene, Po.hyd.

abstract：:Two shuttle vectors named pRC52 (10.7 Kb) and pRK52 (12.2 Kb) carrying chloramphenicol (Cm) and chloramphenicol plus kanamycin (Km) resistance genes, respectively, were constructed by fusion of a cryptic plasmid pBL13869 (replicon pBL1, 5.8 Kb) from Brevibacterium lactofermentum ATCC13869 with pBR328 E. coli plasmid. ...

journal_title：Journal of basic microbiology

authors： Duran R

更新日期：1998-01-01 00:00:00

abstract：:Crude glucoamylase preparation from Rhizoctonia solani was used to saccharify raw and cooked starch. Various concentrations of potato starch and wheat flour from 10-40%, w/v were used for mashing but 30% was found to be the optimal and economical. The saccharified mash yielded 5.89%, v/v ethanol in a simultaneous sacc...

journal_title：Journal of basic microbiology

authors： Singh D,Dahiya JS,Nigam P

更新日期：1995-01-01 00:00:00

abstract：:This study was conducted to determine what effects nanoparticles (NPs) like TiO2 , ZnO, and Ag may pose on natural attenuation processes of petroleum hydrocarbons in contaminated soils. The solid NPs used were identified using x-ray diffraction technique and their average size was certified as 18.2, 16.9, and 18.3 nm ...

journal_title：Journal of basic microbiology

authors： El-Sayed WS,Elbahloul Y,Saad ME,Hanafy AM,Hegazi AH,ElShafei GMS,Elbadry M

更新日期：2019-02-01 00:00:00

abstract：:A total of 216 killer yeasts Saccharomyces cerevisiae, isolated from wine, were evaluated in controlling Colletotrichum gloeosporioides, a pre-harvest anthracnose agent of grape. Three of these yeast isolates were tested positive for antagonizing C. gloeosporioides and were further evaluated for their mechanisms as bi...

journal_title：Journal of basic microbiology

authors： Liu Z,Du S,Ren Y,Liu Y

更新日期：2018-01-01 00:00:00

abstract：:The fungal population of Aswan High Dam Lake showed marked vertical variations during the period of study which extended from July 1985 to December 1986. High fungal counts were observed at the surface water which were mainly due to the high counts of Aspergillus fumigatus and A. terreus. Going deeper the fungal popul...

journal_title：Journal of basic microbiology

authors： Moharram AM,el-Hissy FT,el-Zayat SA

更新日期：1990-01-01 00:00:00

abstract：:This paper deals with the interactions of chromium (Cr) with biological systems, focusing in particular on yeasts and fungi. These interactions are analysed with primarily regard to biochemical functions, but higher levels of organization are also considered. Thus, the morphological and cytological characteristics of ...

journal_title：Journal of basic microbiology

authors： Poljsak B,Pócsi I,Raspor P,Pesti M

更新日期：2010-02-01 00:00:00

abstract：:This study investigates the anticancer cytotoxic mechanism of action of benzoyloxy-ethyl-carbamic acid (BECA) produced by Streptomyces globosus VITLGK011. Flow cytometry analysis confirmed that BECA (at IC50 : 3.12 µg/ml) treatment for 24 h induced apoptosis in 60% of cells. Schrodinger Maestro tools such as QikProp a...

journal_title：Journal of basic microbiology

authors： Lokesh R,Jeyakanthan J,Kannabiran K

更新日期：2020-11-01 00:00:00

abstract：:This paper focuses on isolation and identification of airborne and waterborne fungi from different parts of Terkos Lake located in Istanbul (Turkey). The quantitative and qualitative fungal composition of the air and water of the Lake was surveyed monthly for a year (August 2000-July 2001). Water samples were taken at...

journal_title：Journal of basic microbiology

authors： Asan A,Kirgiz T,Sen B,Camur-Elipek B,Guner U,Guher H

更新日期：2003-01-01 00:00:00

abstract：:A gene encoding an organic solvent-stable protease was amplified from Pseudomonas aeruginosa strain K by polymerase chain reaction using consensus primers based on multiple sequence alignment of alkaline and metalloprotease genes from Pseudomonas species. The gene, which consisted of 1440 bp nucleotides and deduced 47...

journal_title：Journal of basic microbiology

authors： Rahman RN,Geok LP,Wong CF,Basri M,Salleh AB

更新日期：2010-04-01 00:00:00

abstract：:Gamma-hexachlorocyclohexane (gamma-HCH or lindane), one of the most commonly used pesticides, has been mainly used in agriculture; this pesticide is known to be highly toxic and persistent, causing serious water and soil contamination. The objective of the present work is to study the effect of low glucose concentrati...

journal_title：Journal of basic microbiology

authors： Benimeli CS,Castro GR,Chaile AP,Amoroso MJ

更新日期：2006-01-01 00:00:00

abstract：:The present study describes the biosynthesis of proteases by a locally isolated mould culture of Rhizopus oligosporus IHS13 in a low-cost medium by solid-state fermentation technique. The fermentation was carried out in a low-cost medium such as sunflower meal, wheat bran and rice bran. Sunflower meal and wheat bran i...

journal_title：Journal of basic microbiology

authors： Haq IU,Mukhtar H

更新日期：2004-01-01 00:00:00

abstract：:Open reading frame17 (Bm17) of Bombyx mori nucleopolyhedrovirus is a highly conserved gene in lepidopteran nucleopolyhedroviruses, suggesting that it performs an important role in the virus life cycle whose function is unknown. In this report, we describe the characterization of Bm17. Reversed transcriptive-PCR (RT-PC...

journal_title：Journal of basic microbiology

authors： Shen H,Wang R,Han Q,Zhang W,Nin B,Zhou Y,Shao S,Yao Q,Chen K,Liu X

更新日期：2013-10-01 00:00:00

abstract：:Propidium iodide (PI) uptake and salt tolerance assays were used to investigate the mechanism of antibacterial action of an extract of the leaves of Eremophila duttonii, a traditional Australian medicinal plant previously shown to have potent bactericidal activity against Gram positive bacteria. The extract compromise...

journal_title：Journal of basic microbiology

authors： Tomlinson S,Palombo EA

更新日期：2005-01-01 00:00:00

abstract：:The objectives of the present study were to purify and assess the killer toxin effect produced by Aureobasidium pullulans under casual agents of green mold (Penicillum digitatum) and sour rot (Geotrichum citri-aurantii). Initially, different methods of protein precipitation were tested. The proteolytic activity and th...

journal_title：Journal of basic microbiology

authors： Moura VS,Pollettini FL,Ferraz LP,Mazzi MV,Kupper KC

更新日期：2020-12-29 00:00:00

abstract：:The gene mak1FN coding for maltokinase from Actinoplanes missouriensis is located in a cluster similar to glycogen metabolism clusters identified in Streptomyces coelicolor. Sequence comparisons demonstrate that mak1-related genes coding for homologous proteins are present in many bacterial genomes including taxonomic...

journal_title：Journal of basic microbiology

authors： Jarling M,Cauvet T,Grundmeier M,Kuhnert K,Pape H

更新日期：2004-01-01 00:00:00

abstract：:The mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (FAD-GPDH), recently reported in plants, has been detailed in yeast and animal systems. It oxidizes glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) on the outer surface of mitochondrial inner membrane. A cDNA encoding the Dunaliella s...

journal_title：Journal of basic microbiology

authors： Yang W,Cao Y,Sun X,Huang F,He Q,Qiao D,Bai L

更新日期：2007-06-01 00:00:00

abstract：:The high-level expression of the xylanase GH11 gene from Aspergillus niger IA-001 called xynB was successfully completed in Pichia pastoris. The xynB gene encoding a mature xylanase of 225 amino acid was subcloned into the pPICZαA vector and was transformed into P. pastoris X-33 under the control of the alcohol oxidas...

journal_title：Journal of basic microbiology

authors： Fang W,Gao H,Cao Y,Shan A

更新日期：2014-07-01 00:00:00

abstract：:L-form strains of Proteus mirabilis and Escherichia coli lacking the cell wall represent an alternative prokaryotic cell system for the production of recombinant proteins (KLESSEN et al. 1988, LAPLACE et al. 1988a, 1989b). We could demonstrate that they are also able to synthesize the enzyme penicillin G acylase (PAC)...

journal_title：Journal of basic microbiology

authors： Gumpert J,Cron H,Plapp R,Niersbach H,Hoischen C

更新日期：1996-01-01 00:00:00

abstract：:The aim of this study was to identify genes involved in biofilm development in the probiotic lactic acid bacterium Lactobacillus plantarum. The ability of L. plantarum LM3 and of some derivative mutant strains to form biofilm has been investigated. Biofilm microtitre plate assays showed that L. plantarum LM3-2, carryi...

journal_title：Journal of basic microbiology

authors： Muscariello L,Marino C,Capri U,Vastano V,Marasco R,Sacco M

更新日期：2013-01-01 00:00:00

abstract：:In the course of a screening for extracellular inhibitors of 8 different proteinases 140 actinomycete and 150 mould strains were examined. 130 fungal and 50 actinomycete strains were similarly tested for the production of extracellular inhibitors of dihydrofolate reductase (DHFR) and alkaline phosphatase (APh). Most o...

journal_title：Journal of basic microbiology

authors： Angelov T,Angelova B,Kourteva Y,Stephanova L,Ayramova T,Peretz V,Konova O

更新日期：1989-01-01 00:00:00

abstract：:Epiphytic microbes are common inhabitants of freshwater submerged macrophytes, which play an important role in aquatic ecosystems. An important precondition for studying the epiphytic microbes is having an effective method of separating the attached microbes from the host macrophytes. We developed an ultrasound-based ...

journal_title：Journal of basic microbiology

authors： Cai X,Gao G,Yang J,Tang X,Dai J,Chen D,Song Y

更新日期：2014-07-01 00:00:00

abstract：:The recA gene of Proteus mirabilis (recApm) has been cloned into the PstI site of the Bacillus promoter-probe plasmid pPL603. When present on this plasmid, the recApm1) gene is expressed in B. subtilis under the control of its own transcriptional and translational signals. It is concluded that the high AT-content of t...

journal_title：Journal of basic microbiology

authors： Eitner-Mönke G,Manteuffel R

更新日期：1986-01-01 00:00:00

abstract：:Some bacteria have a higher tendency to produce biofilm than others. Especially, Pseudomonas and Aeromonas strains are acknowledged to be pioneer colonizers and are predominant in biofilm formation. We examined biofilm formation and first attachment maintance of biofilms of Pseudomonas spp., Pseudomonas aeruginosa, Ae...

journal_title：Journal of basic microbiology

authors： Doğruöz N,Göksay D,Ilhan-Sungur E,Cotuk A

更新日期：2009-09-01 00:00:00

abstract：:To understand the physiology of non-differentiating bacteria exposed to nutrient deprivation and stress, various approaches have been employed in combination with detailed analysis of protein synthesis pattern. In this study, separation of proteins from clarified cell extracts of Pseudomonas aeruginosa PAO1 grown unde...

journal_title：Journal of basic microbiology

authors： Madhusudhan KT,McLaughlin R,Komori N,Matsumoto H

更新日期：2003-01-01 00:00:00

abstract：:Clinical isolates of Proteus penneri were tested for the presence of genes encoding hemolytic activity. Strains possessing DNA sequences similar to the hlyCABD genes in Escherichia coli were found. Each secreted a 110 kDa protein which reacted with a specific anti-HlyA antiserum. Southern blotting analysis revealed th...

journal_title：Journal of basic microbiology

authors： Serwecinska L,Pytlos M,Lukomski S,Jaworski A,Rózalski A

更新日期：1997-01-01 00:00:00

abstract：:Ammonia oxidation, mainly driven by ammonia-oxidizing archaea (AOA) and bacteria (AOB), plays an important role in determining the rate of nitrification in riparian zones. However, the underlying factors driving the distribution and activity of AOA and AOB in riparian zones, especially in the rhizosphere of Phragmites...

journal_title：Journal of basic microbiology

authors： Wang X,Wang S,Shi G,Wang W,Zhu G

更新日期：2019-04-01 00:00:00

abstract：:A 2-haloacid dehalogenase-producing bacterium, designated DEH99, was isolated from the marine sponge Hymeniacidon perlevis using a modified enrichment medium and a pH indicator method. DEH99 could degrade only half of the racemic mixture 2-chloropropionic acid (2-CPA) in the medium. The dehalogenase of DEH99 was furth...

journal_title：Journal of basic microbiology

authors： Huang J,Xin Y,Zhang W

更新日期：2011-06-01 00:00:00

abstract：:In vitro effect of salivary gland extract from fed Ixodes ricinus, the competent vector of Lyme borreliosis in Europe, on the growth of Borrelia burgdorferi sensu lato (B. garinii, B. afzelii and B. burgdorferi sensu stricto) was examined in BSK-H medium. Motility rate, concentration of motile spirochetes and their mo...

journal_title：Journal of basic microbiology

authors： Rudolf I,Sikutová S,Kopecký J,Hubálek Z

更新日期：2010-06-01 00:00:00

abstract：:An outbreak of serious mortality among the cultured cobia Rachycentron canadum (weighing 3 kg) characterized by the presence of whitish granulomatous deposits on the kidney, liver and spleen occurred in July of 2000 in Taiwan. A non-motile strain CP1 was isolated from kidney and/or liver on tryptic soy agar and/or bra...

journal_title：Journal of basic microbiology

authors： Liu PC,Lin JY,Lee KK

更新日期：2003-01-01 00:00:00

abstract：:Heterotrophic bacteria (11 genera, 14 species, 25 putative strains) were isolated from drinking water, identified either biochemically or by partial 16s rDNA gene sequencing and their adherence characteristics were determined by two methods: i. thermodynamic prediction of adhesion potential by measuring hydrophobicity...

journal_title：Journal of basic microbiology

authors： Simões LC,Simões M,Oliveira R,Vieira MJ

更新日期：2007-04-01 00:00:00