Synthesis and secretion of recombinant penicillin G acylase in bacterial L-forms.

abstract：:The objective was to screen for and isolate a novel enzyme with the specific activity of a Δ6-fatty acid desaturase from Rhizopus oryzae. In this study, R. oryzae was identified as a novel fungal species that produces large amounts of γ-linolenic acid. A full-length cDNA, designated here as RoD6D, with high homology t...

journal_title：Journal of basic microbiology

authors： Zhu Y,Zhang BB

更新日期：2013-09-01 00:00:00

abstract：:This paper focuses on isolation and identification of airborne and waterborne fungi from different parts of Terkos Lake located in Istanbul (Turkey). The quantitative and qualitative fungal composition of the air and water of the Lake was surveyed monthly for a year (August 2000-July 2001). Water samples were taken at...

journal_title：Journal of basic microbiology

authors： Asan A,Kirgiz T,Sen B,Camur-Elipek B,Guner U,Guher H

更新日期：2003-01-01 00:00:00

abstract：:This study evaluated ethanol fermentation and its correlation with glutathione (GSH) synthesis under various cadmium-conditions with different metal ions and nitrogen sources. We found that corn steep liquor (CSL) and yeast extract have differential roles to play in GSH accumulation in cell even though both of them co...

journal_title：Journal of basic microbiology

authors： Xu Q,Wu M,Hu J,Gao MT

更新日期：2016-01-01 00:00:00

abstract：:Total cellular proteins and fatty acid composition profiles of mid-log phase cells of Salmonella enterica serovar Typhimurium grown at 8, 25, 37 or 42 °C were separated by 2D-PAGE and FAME analysis. Growth temperature associated protein expression can be grouped into 3 thermal classes which include proteins whose expr...

journal_title：Journal of basic microbiology

authors： Balamurugan S,Dugan ME

更新日期：2010-12-01 00:00:00

abstract：:Studies on Acidithiobacillus ferrooxidans accepting electrons from Fe(II) have previously focused on cytochrome c. However, we have discovered that, besides cytochrome c, type IV pili (Tfp) can transfer electrons. Here, we report conduction by Tfp of A. ferrooxidans analyzed with a conducting-probe atomic force micros...

journal_title：Journal of basic microbiology

authors： Li Y,Li H

更新日期：2014-03-01 00:00:00

abstract：:Mn-oxidizing potential of two metal-tolerant bacterial strains - Halomonas meridiana and Marinobacter algicola isolated from the South West Indian Ridge waters were compared at varying concentrations of Mn (II), i.e., 1, 10, and 100 μmol and mmol L-1 . Accompanying changes in their morphology and metabolism were also ...

journal_title：Journal of basic microbiology

authors： Fernandes SO,Surya Prakash L,Balan Binish M,Padinchati Krishnan K,John Kurian P

更新日期：2018-05-01 00:00:00

abstract：:Sphingobium sp. SYK-6 can utilize lignin biphenyl compounds, such as 2,2'-dihydroxy-3,3'-dimethoxy-5,5'-dicarboxybiphenyl (DDVA). In the metabolism of DDVA, this microorganism exploits two O -demethylation systems with different substrate specificities, namely, a DDVA-specific oxygenative O -demethylation system and a...

journal_title：Journal of basic microbiology

authors： Sonoki T,Masai E,Sato K,Kajita S,Katayama Y

更新日期：2009-09-01 00:00:00

abstract：:alpha-Aminodipate, an intermediate of the lysine biosynthetic pathway of fungi, or lysine when used as the sole nitrogen source in the medium was growth inhibitory and toxic to Saccharomyces cerevisiae. The fission yeast Schizosaccharomyces pombe and pathogenic fungi Candida albicans, Filobasidiella neoformans and Asp...

journal_title：Journal of basic microbiology

authors： Ye ZH,Garrad RC,Winston MK,Bhattacharjee JK

更新日期：1991-01-01 00:00:00

abstract：:Cassava wastes--the peel and the root fibre were taken through various pretreatment procedures before being subjected to solid state fermentation with Trichoderma harzianum. Most of the pretreatment processes increased the cellulose and hemicellulose content of the cassava peel and fibre by as high as 155% while sulfu...

journal_title：Journal of basic microbiology

authors： Onilude AA

更新日期：1996-01-01 00:00:00

abstract：:Spodoptera littoralis, one of the major pests of many important crop plants, is more susceptible to Bacillus thuringiensis aizawai delta-endotoxins than to those of Bacillus thuringiensis kurstaki. Within the framework of the development of efficient bioinsecticides and the prevention against insect resistance, we tes...

journal_title：Journal of basic microbiology

authors： Benfarhat-Touzri D,Ben Amira A,Ben khedher S,Givaudan A,Jaoua S,Tounsi S

更新日期：2014-11-01 00:00:00

abstract：:To explore the influence of seasonal and regional environmental factors on the bacterial community composition (BCC) and diversity in the sediments of Lake Bosten, the 16S rRNA gene of sediment bacteria in four samples from the lake center area and the macrophyte-dominated area were sequenced using 454 pyrosequencing....

journal_title：Journal of basic microbiology

authors： Zhang L,Zhao T,Shen T,Gao G

更新日期：2019-02-01 00:00:00

abstract：:Protein turnover affects protein abundance and phenotypes. Comprehensive investigation of protein turnover dynamics has the potential to provide substantial information about gene expression. Here we report a large-scale protein turnover study in Salmonella Typhimurium during infection by quantitative proteomics. Muri...

journal_title：Journal of basic microbiology

authors： Wang Z,Han QQ,Zhou MT,Chen X,Guo L

更新日期：2016-07-01 00:00:00

abstract：:Mixed bacterial cultures are increasingly being used in the production of polyhydroxyalkanoates (PHAs), as they have the potential to be more cost effective than axenic pure cultures. The purpose of this study was to use pure cultures in combination to identify their potential of PHA production. In this work we used v...

journal_title：Journal of basic microbiology

authors： Munir S,Jamil N

更新日期：2018-03-01 00:00:00

abstract：:Supercoiling of bacterial DNA is regulated by topoisomerases and influences most of the metabolic processes involving DNA. The present review is devoted to a brief outline of the supercoiled state of DNA in bacteria and to all microbial topoisomerases hitherto described. Recent studies on topoisomerases of archaebacte...

journal_title：Journal of basic microbiology

authors： Zimmer C,Störl K,Störl J

更新日期：1990-01-01 00:00:00

abstract：:For mass cultivation of group B streptococci (B-III and B-variant streptococci), a fermenter dialysis culture technique is described and compared to conventional shaking culture and fermenter batch culture techniques. The influence of two kinds of cultivation media on the bacterial yield is demonstrated. The growth ra...

journal_title：Journal of basic microbiology

authors： Starke R,Mehl M,Möckel C,Grunow R,Montag T,Von Baehr R,Schmidt G

更新日期：1989-01-01 00:00:00

abstract：:The Anoxybacillus sp. SK 3-4, previously isolated from a hot spring, was screened for its heavy metals resistance (Al(3+), Mn(2+), Cu(2+), Co(2+), Zn(2+), and Ni(2+)) and the strain was found to be most resistant to aluminum. Significant growth of the strain was observed when it was grown in medium containing aluminum...

journal_title：Journal of basic microbiology

authors： Lim JC,Goh KM,Shamsir MS,Ibrahim Z,Chong CS

更新日期：2015-04-01 00:00:00

abstract：:Epiphytic microbes are common inhabitants of freshwater submerged macrophytes, which play an important role in aquatic ecosystems. An important precondition for studying the epiphytic microbes is having an effective method of separating the attached microbes from the host macrophytes. We developed an ultrasound-based ...

journal_title：Journal of basic microbiology

authors： Cai X,Gao G,Yang J,Tang X,Dai J,Chen D,Song Y

更新日期：2014-07-01 00:00:00

abstract：:Pichia pastoris expression system was widely used for producing heterologous proteins. Some researches revealed that the increase in the copy number could improve the expression of foreign genes in P. pastoris. Hundreds or thousands of antibiotic-resistance recombinants need to be screened because the frequency of mul...

journal_title：Journal of basic microbiology

authors： Zheng J,Guo N,Zhou HB

更新日期：2014-12-01 00:00:00

abstract：:Two strains of 64 endophytic bacteria, Bacillus cereus Si-Ps1 and Pseudomonas azotoformans La-Pot3-3, isolated from Citrus sinensis and C. sinensis var. Thomson's leaves, respectively, reduced N-acyl homoserine-based quorum sensing in bioindicator strain Pseudomonas syringae pv. syringae (Pss) B728a and the biofilm pr...

journal_title：Journal of basic microbiology

authors： Akbari Kiarood SL,Rahnama K,Golmohammadi M,Nasrollanejad S

更新日期：2020-09-01 00:00:00

abstract：:Five-hundreds and eighty bacterial strains, isolated from various Antarctic marine sources and locations, were screened for antimicrobial activity against terrestrial microorganisms. Twenty-two Antarctic isolates (3.8%), mainly retrieved from the water column at Terra Nova Bay (Ross Sea), expressed antagonistic activi...

journal_title：Journal of basic microbiology

authors： Lo Giudice A,Bruni V,Michaud L

更新日期：2007-12-01 00:00:00

abstract：:Yarrowia lipolytica yeast was grown batchwise on n-hexadecane as the carbon and energy source. Two cerebroside species were quantitatively isolated from sphingolipid fractions of total lipids by a combination of column chromatography and preparative high-performance thin-layer chromatography. The cerebroside content a...

journal_title：Journal of basic microbiology

authors： Rupcić J,Georgiù K,Marić V

更新日期：2004-01-01 00:00:00

abstract：:The analysis of RubisCO genes is a highly useful instrument to explore the diversity of chemoautotrophic bacteria using the Calvin-Benson-Bassham cycle for CO2 fixation. However, because of the wide taxonomic distribution of phylogenetically related RubisCO forms, environmental studies targeting chemoautotrophs are ha...

journal_title：Journal of basic microbiology

authors： Alfreider A,Bogensperger T

更新日期：2018-08-01 00:00:00

abstract：:Thermus strains are regarded as models to investigate the mechanism of thermostability of thermophiles, and phages from Thermus are particularly interesting because of their way to regulate gene expression. In this research, a Thermus bacteriophage named TSP4 (Thermus Siphoviridae phage) was isolated from Tengchong ho...

journal_title：Journal of basic microbiology

authors： Lin L,Hong W,Ji X,Han J,Huang L,Wei Y

更新日期：2010-10-01 00:00:00

abstract：:The present study demonstrates cloning, expression, and characterization of hyperthermostable L-asparaginase from Thermococcus kodakarensis KOD1 in Escherichia coli BLR(DE3). The recombinant 6× His-tagged protein L-asparaginase from T. kodakarensis (TkAsn), was purified to homogeneity by heat treatment followed by aff...

journal_title：Journal of basic microbiology

authors： Hong SJ,Lee YH,Khan AR,Ullah I,Lee C,Park CK,Shin JH

更新日期：2014-06-01 00:00:00

abstract：:A simple photometric method for the determination of the agglutination rate of cells by lectins in a continuously stirred suspension is presented. Besides the agglutination rate the method allows the estimation of an average degree of agglutination, i.e. the number of cells per aggregate. The influence of the Con A-co...

journal_title：Journal of basic microbiology

authors： Flemming C,Gabert A,Flemming I

更新日期：1985-01-01 00:00:00

abstract：:The effects of cold-shock on Neurospora crassa was here investigated. In cold-shock treated hyphae (6 h at 4 degrees C) the branching pattern was changed and dichotomy as well as a fan-like-pattern structure were temporarily detected at the tips. When exogenous Ca(2+) (500 mM) was added to the hyphae, these morphologi...

journal_title：Journal of basic microbiology

authors： Kawano CY,Said S

更新日期：2002-01-01 00:00:00

abstract：:Members of Bacillaceae, Rhizobiaceae, actinomycetes and others were isolated from cultivated and non-cultivated saline soils. The high population of bacteria and actinomycetes were almost coincided with the relatively high levels of organic matter whatever the degree of soil salinity. Bacillus stearothermophilus and B...

journal_title：Journal of basic microbiology

authors： Zahran HH,Moharram AM,Mohammad HA

更新日期：1992-01-01 00:00:00

abstract：:Dietary carbohydrates and polyols affect the microbial colonization of oral surfaces by modulating adhesion and biofilm formation. The aim of this study was to evaluate the influence of a select group of l-carbohydrates and polyols on either Streptococcus mutans or Candida albicans adhesion and biofilm formation in vi...

journal_title：Journal of basic microbiology

authors： Brambilla E,Ionescu AC,Cazzaniga G,Ottobelli M,Samaranayake LP

更新日期：2016-05-01 00:00:00

abstract：:Microorganisms such as bacteria and molds produce an enormous variety of volatile metabolites. To determine whether typical microbial volatile metabolites can be used as indicator compounds for the detection of hidden mold in indoor environments, we examined 14 typical indoor fungal strains for their growth rates and ...

journal_title：Journal of basic microbiology

authors： Schuchardt S,Kruse H

更新日期：2009-08-01 00:00:00

abstract：:To investigate the influence of culture conditions on growth of filamentous bacteria in submerged culture, S. tendae was cultivated at various temperatures and in various media. For this purpose, a temperature-controlled growth chamber was constructed, which allows the cultivation of filamentous bacteria and fungi oxy...

journal_title：Journal of basic microbiology

authors： Reichl U,King R,Gilles ED

更新日期：1992-01-01 00:00:00