Abstract:
:As many as 71 marine bacterial DNA extracts were PCR screened for L-asparaginase (ansA), a key gene in anti-cancer molecular-searches. Over 62% (44) of them were positive for ansA gene. The positive cultures were from genera Bacillus and Staphylococcus. The ansA gene cloned from isolate NIOS4 belonging to recently described Bacillus tequilensis is 1099 bp in length with a 990 bp ORF coding for 329 amino acids. BLASTx analysis revealed this sequence to be 98% similar to earlier reported ansA sequence from B. subtilis (Accession no. NP390239.1). By comparing its deduced amino acid sequence with other bacterial asparaginase sequences six substitutions at positions 305(Thr), 313(Lys), 314(Leu), 315(Asp), 318(Arg), and 320(Gln) are observed. Key residues like Thr(12), Thr(85), Asp(86), Lys(156), and Phe(165) taking part in active-site formation and imparting catalytic properties are conserved. The phylogenetic tree based of the ansA amino acid sequences revealed close relatedness of the NIOS4 ansA sequence with B. subtilis (Accession no. NP 390239.1). It's very close genetic resemblance to B. subtilis and conservation of certain key amino acid residues suggest it as a prospective candidate for evaluation and, production of L-asparaginases.
journal_name
J Basic Microbioljournal_title
Journal of basic microbiologyauthors
Nayak S,Porob S,Fernandes A,Meena RM,Ramaiah Ndoi
10.1002/jobm.201200355subject
Has Abstractpub_date
2014-02-01 00:00:00pages
162-8issue
2eissn
0233-111Xissn
1521-4028journal_volume
54pub_type
杂志文章abstract::Insecticidal proteins expressed by genetically modified Bt maize may alter the enzymatic and microbial communities associated with rhizosphere soil. This study investigated the structure and enzymatic activity of rhizosphere soil microbial communities associated with field grown Bt and non-Bt maize. Rhizosphere soil s...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201700043
更新日期:2017-09-01 00:00:00
abstract::This study focuses on analyzing the protein expression pattern of intracellular proteins when Pseudomonas mendocina SMSKVR-3 exposed to 300 mM of arsenate to find out the proteins that are overexpressed or exclusively expressed in response to arsenate. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis anal...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.202000671
更新日期:2021-01-14 00:00:00
abstract::The gene mak1FN coding for maltokinase from Actinoplanes missouriensis is located in a cluster similar to glycogen metabolism clusters identified in Streptomyces coelicolor. Sequence comparisons demonstrate that mak1-related genes coding for homologous proteins are present in many bacterial genomes including taxonomic...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200410403
更新日期:2004-01-01 00:00:00
abstract::Penicillium chrysogenum secretes a low molecular weight, cationic and cysteine-rich protein (PAF). It has growth inhibitory activity against the model organism Aspergillus nidulans and numerous zoo- and phytopathogenic fungi but shows only minimal conditional antifungal activity against the producing organism itself. ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201000321
更新日期:2011-06-01 00:00:00
abstract::An extracellular endoglucanase (EG) that catalyzes the hydrolysis of carboxy-methyl cellulose (CMC) as substrate was purified to homogeneity from the soft-rot causing oomycete P. myriotylum with maximum EG production observed in presence of 1% (w/v) sucrose. The enzyme designated PmEG was observed to be monomeric with...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201400323
更新日期:2014-12-01 00:00:00
abstract::Studies on Acidithiobacillus ferrooxidans accepting electrons from Fe(II) have previously focused on cytochrome c. However, we have discovered that, besides cytochrome c, type IV pili (Tfp) can transfer electrons. Here, we report conduction by Tfp of A. ferrooxidans analyzed with a conducting-probe atomic force micros...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201200300
更新日期:2014-03-01 00:00:00
abstract::Optimization of indigo production process from indole using a newly isolated phenol-degrading bacterial strain was performed by Plackett-Burman design and response surface methodology. The strain designated as QM was identified as Pseudomonas sp. according to 16S rDNA analysis. Spectrum analysis of indole biotransform...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201100516
更新日期:2012-12-01 00:00:00
abstract::Trichoderma spp. are versatile beneficial fungi which can stimulate growth and plant resistance to biotic and abiotic stresses. In this study, the potential of Trichoderma isolate in promoting the cucumber growth under salt stress and its possible mechanisms were investigated. Strain Q1 was isolated from the rhizosphe...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201200031
更新日期:2013-04-01 00:00:00
abstract::An extracellular lipase isolated from Bacillus megaterium AKG-1 had an optimum activity at 55 degrees C/pH 7.0. It retained 100% activity at 50 degrees C for 30 min with a half life of 30 min at 70 degrees C. A 20-70% increase in lipase activity was observed in presence of acetone (20% v/v), DMSO (20% v/v) and isoprop...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200410498
更新日期:2005-01-01 00:00:00
abstract::Studies were carried out to isolate chlorpyrifos degrading Bacillus thuringiensis (Bt) strains from chlorpyrifos-contaminated samples. Six Bt strains (isolation rate 2.7%) were isolated by modified sodium acetate antibiotic heat treatment, and one novel strain (BRC-HZM2) was selected for further analysis. Phenotype an...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201300501
更新日期:2015-03-01 00:00:00
abstract::This study was conducted to determine what effects nanoparticles (NPs) like TiO2 , ZnO, and Ag may pose on natural attenuation processes of petroleum hydrocarbons in contaminated soils. The solid NPs used were identified using x-ray diffraction technique and their average size was certified as 18.2, 16.9, and 18.3 nm ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201800186
更新日期:2019-02-01 00:00:00
abstract::Crude glucoamylase preparation from Rhizoctonia solani was used to saccharify raw and cooked starch. Various concentrations of potato starch and wheat flour from 10-40%, w/v were used for mashing but 30% was found to be the optimal and economical. The saccharified mash yielded 5.89%, v/v ethanol in a simultaneous sacc...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620350209
更新日期:1995-01-01 00:00:00
abstract::For mass cultivation of group B streptococci (B-III and B-variant streptococci), a fermenter dialysis culture technique is described and compared to conventional shaking culture and fermenter batch culture techniques. The influence of two kinds of cultivation media on the bacterial yield is demonstrated. The growth ra...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620290316
更新日期:1989-01-01 00:00:00
abstract::Yarrowia lipolytica yeast was grown batchwise on n-hexadecane as the carbon and energy source. Two cerebroside species were quantitatively isolated from sphingolipid fractions of total lipids by a combination of column chromatography and preparative high-performance thin-layer chromatography. The cerebroside content a...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200310337
更新日期:2004-01-01 00:00:00
abstract::A mosquitocidal soil isolate of Bacillus thuringiensis serovar fukuokaensis (H3ade) produced spherical parasporal inclusions measuring 0.6-0.7 microm in diameter. Inclusion matrix was homogeneous substance surrounded by a thick, highly electron-dense envelope 30-50 nm in thickness. The envelopes were associated with b...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200510010
更新日期:2006-01-01 00:00:00
abstract::Iron deficiency causes oversynthesis of riboflavin in several yeast species, known as flavinogenic yeasts. However, the mechanisms of such regulation are not known. We found that mutations causing riboflavin overproduction and iron hyperaccumulation (rib80, rib81 and hit1), as well as cobalt excess or iron deficiency ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.200610279
更新日期:2007-10-01 00:00:00
abstract::Shigella adhesion to host cells is a transitional stage from an extracellular to an intracellular environment. However, the dynamic adaptations of Shigella during adhesion are poorly understood. To address this, we performed the first transcriptome analysis of Shigella flexneri 2457T during adhesion. A total of 1,757 ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201400414
更新日期:2015-02-01 00:00:00
abstract::Enzymes of the glutathione-dependent detoxification pathway (glutathione S-transferase and gamma-glutamyl-transpeptidase) were induced, and the glutathione pool was completely depleted by phenoxyacetic acid in Penicillium chrysogenum mycelia incubated for 15 h in a culture medium containing lactose as a carbon source ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620370306
更新日期:1997-01-01 00:00:00
abstract::Aspergillus nidulans produces sterigmatocystin, a secondary metabolite mycotoxin, for the protection of its reproductive structures. Previous studies on grazing behavior of fungivore arthropods, regulation of sexual development, and secondary metabolite biosynthesis have revealed the association of sterigmatocystin bi...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201800020
更新日期:2018-07-01 00:00:00
abstract::Endophytes represent a hidden world within plants. Almost all plants that are studied harbor one or more endophytes, which help their host to survive against pathogens and changing adverse environmental conditions. Fungal and bacterial endophytes with distinct ecological niches show important biological activities and...
journal_title:Journal of basic microbiology
pub_type: 杂志文章,评审
doi:10.1002/jobm.201700275
更新日期:2017-10-01 00:00:00
abstract::The present study was carried out to examine the ability of four species of the genus Phlebia, viz. P. floridensis, P. brevispora, P. radiata and P. fascicularia, to produce the ligninolytic enzyme laccase in liquid culture. P. floridensis was the most active species that even surpassed laccase production by Trametes ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/1521-4028(200210)42:5<295::AID-JOBM295>3.0
更新日期:2002-01-01 00:00:00
abstract::Abscisic acid (ABA) plays important roles in many aspects of plant growth and development. Botrytis cinerea TB-3-H8, a high-yield strain of ABA, was used to elucidate the molecular mechanisms of ABA production in the present work. cDNA-amplified fragment length polymorphism (cDNA-AFLP) technique was applied to isolate...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201200311
更新日期:2014-03-01 00:00:00
abstract::Two shuttle vectors named pRC52 (10.7 Kb) and pRK52 (12.2 Kb) carrying chloramphenicol (Cm) and chloramphenicol plus kanamycin (Km) resistance genes, respectively, were constructed by fusion of a cryptic plasmid pBL13869 (replicon pBL1, 5.8 Kb) from Brevibacterium lactofermentum ATCC13869 with pBR328 E. coli plasmid. ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::A soluble, NADP+-dependent alcohol dehydrogenase (ADH) with a pH optimum at 8.7 was found in A. calcoaceticus EB 104 after growth on different carbon sources. n-Alkanols with short and medium chain length were employed as test substrates. The Km values decreased with increasing chain length. The Vmax values remained n...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.3620260611
更新日期:1986-01-01 00:00:00
abstract::Epiphytic microbes are common inhabitants of freshwater submerged macrophytes, which play an important role in aquatic ecosystems. An important precondition for studying the epiphytic microbes is having an effective method of separating the attached microbes from the host macrophytes. We developed an ultrasound-based ...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201300041
更新日期:2014-07-01 00:00:00
abstract::The acetic acid bacteria have mainly relevance for bacterial cellulose production and fermented bio-products manufacture. The purpose of this study was to identify temperate bacteriophages in a cellulose-producing bacterial strain Komagataeibacter intermedius IMBG180. Prophages from K. intermedius IMBG180 were induced...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201400711
更新日期:2015-04-01 00:00:00
abstract::A comparative electron microscopic analysis of weakly beta-haemolytic spirochaetes related to human and animal intestinal spirochaetosis was done in order to search for the presence of inducible bacteriophages associated with these spirochaetes. Bacteriophages were detected at the electron microscope after experimenta...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Many ecosystems are currently co-contaminated with heavy metals such as cadmium (Cd(2+) ) and pesticides such as chlorpyrifos (CP) and γ-hexachlorocyclohexane (γ-HCH). A feasible approach to remediate the combined pollution of heavy metals and pesticides is the use of γ-HCH degrading bacteria endowed with CP hydrolysi...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201500559
更新日期:2016-07-01 00:00:00
abstract::The objective was to screen for and isolate a novel enzyme with the specific activity of a Δ6-fatty acid desaturase from Rhizopus oryzae. In this study, R. oryzae was identified as a novel fungal species that produces large amounts of γ-linolenic acid. A full-length cDNA, designated here as RoD6D, with high homology t...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.201200189
更新日期:2013-09-01 00:00:00
abstract::This study investigates the anticancer cytotoxic mechanism of action of benzoyloxy-ethyl-carbamic acid (BECA) produced by Streptomyces globosus VITLGK011. Flow cytometry analysis confirmed that BECA (at IC50 : 3.12 µg/ml) treatment for 24 h induced apoptosis in 60% of cells. Schrodinger Maestro tools such as QikProp a...
journal_title:Journal of basic microbiology
pub_type: 杂志文章
doi:10.1002/jobm.202000461
更新日期:2020-11-01 00:00:00